54results about How to "Helps to filter" patented technology

The invention relates to the technical field of molecular breeding, in particular to molecular markers from sea island cotton Hai 1 and related to cotton fiber strength and application of the molecular markers. The molecular markers includes HAU1980240, NAU3177160, CGR5149280, NAU3405220 and NAU1325210. By the molecular markers, defects of current breeding techniques in fiber quality identification are overcome, fiber strength selection efficiency can be increased, and breeding process of new varieties of high-quality fibers can be accelerated.

The invention provides an efficient cooperative positioning method applied to a mobile ad hoc network. The method includes the following steps that: step A, a corresponding reference node set is determined according to a current target node, and distance measurement communication with corresponding reference nodes is carried out; step B, a certain number of position estimation samples for the target node are generated randomly according to distance measurement values and a distance measurement error model; step C, the position estimation samples generated by each reference node are screened separately according to weighting values, a screened-out sample is adopted as the confidence of the target node in current iteration, the estimated position of the target node in the current iteration is calculated; and step D, whether the iteration process of the current target node converges or reaches a maximum number is judged, if the iteration process of the current target node converges, the current target node is adopted as a reference node of another target node to be positioned. With the efficient cooperative positioning method adopted, the convergence speed of the iteration process of the target node is high, and computation complexity is low, high positioning accuracy can be ensured.

The invention relates to the field of medical instruments and relates to a spasticity evaluation system and device based on the myotatic reflex threshold value and the resistance variable. The device comprises a detection head, a surface electromyogram, a computer and a display screen. The detection head integrates pressure, angle, speed and acceleration sensors and is connected with the computer through a data line or Bluetooth. The surface electromyogram records the electromyographic signals of measured muscles through surface electrodes. The computer analyzes the limb resistance variable, the stretching activity speed and the acceleration generated during a stretching activity and records the joint angle of triggering of myotatic reflex. A linear regression equation of the stretching speed-joint angle of triggering of myotatic reflex is established and the joint angle-myotatic reflex threshold value on the condition of the theoretical stretching speed 0 is obtained. The display screen can display the stretching speed animation preview, the myoelectric activity and the stretching resistance change. The evaluation system and device can solve the problem in accurate evaluation of the upper motor neuron syndrome (UMNS) in rehabilitation medicine, can better perform quantitative evaluation of spasticity therein, and facilitate further selection of reasonable intervention projects.

The invention relates to the technical field of molecular breeding, in particular to molecular markers derived from sea island cotton Hai 1 and associated with the fiber length and applications of the molecular markers. The molecular markers are PGML01861(330), DPL0209(200) and CGR5565(150). According to the molecular markers and the applications, the defects of conventional breeding technology for fiber quality identification are overcome, the fiber length selection efficiency can be improved, and the cultivation process of a new high-quality variety is accelerated.

The invention provides a rhesus monkey temporal lobe epilepsy kindling model. In the model, coriamyrtin with the subthreshold seizure dose of 0.5 ml/kg is applied in rhesus monkey, and the effect of 1ml of coriamyrtin injection is equivalent to that of 6.7g of raw material medicine Chinese coriaria leaf. The invention also provides a method for screening drugs for curing temporal lobe epilepsy. The method of the invention has the following advantages: the method is simple to establish and easy to operate and repeat, does not cause mechanical damage on the brain, has short seizure time, etc. The model of the invention resists the classical antiepileptic drug carbamazepine, thus the model can be used in the research on the pathogenesis of the temporal lobe epilepsy and the research on the development of new antiepileptic drugs.

The invention belongs to the field of biotechnology, and particularly relates to a SNP molecular marker for detecting lobed-leaf trait of brassica campestris vegetables and applications thereof. The SNP molecular marker is selected from: (a) the 358th locus of a template strand of Bra009510 gene, wherein the rna polymorphism is G or A; and (b) a homologous gene homologous to the Bra009510 in a brassica campestris vegetable variety with an integrifolious trait maintained, wherein the rna polymorphism corresponding to the 358th locus of SEQ ID NO.1 is G, and/or a homologous gene homologous to the Bra009510 in a brassica campestris variety with a lobed-leaf trait, wherein the rna polymorphism corresponding to the 358th locus of the SEQ ID NO.1 is A. The brassica campestris vegetable variety with an integrifolious trait maintained and/or the brassica campestris variety with a lobed-leaf trait are selected from: natural varieties, hybrid varieties and artificial mutagen varieties. The sequence of the template strand of the Bra009510 gene is as shown in the SEQ ID NO.1. The molecular marker provided by the invention and corresponding primers have a selection accuracy of 100% whether in lobed-leaf or integrifolious materials, and can be used for molecular marker-assisted selection breeding.

The invention belongs to the fields of environmental microbiology, microbiological detection and quantity monitoring, and relates to a culture medium formula ingredient for effectively meeting hydrogen sulfide producing bacterium growth and promoting metabolism and reproduction and application thereof. The culture medium is prepared from basic culture medium solution, microelement liquid, vitaminliquid and the like, and also contains nutritional ingredients such as compound organic carbon sources and sulfur sources. Under the anaerobic condition, a proper volume of anaerobic collected oil field environment water samples (such as produced water) are taken and are inoculated into the culture medium; anaerobic culture is performed under the condition with the same temperature as the environment sample in-situ temperature; the growth and the metabolism of various microorganism physiological populations with hydrogen sulfide producing capability in the samples can be effectively promoted.When the culture medium is used, and meanwhile, other anaerobic microbiological methods are combined, the strain enrichment, the separation and screening and the microbiological quantity detection andmonitoring of the hydrogen sulfide producing bacterium in the samples from oil field environment or other environment can be effectively realized.

The invention discloses an SNP molecular marker for identifying and controlling formation of low-temperature yellowing property of Chinese cabbage leaves and an application thereof. An SNP site provided by the invention is the 300th nucleotide from terminal 5' of a sequence 4 in a sequence table of a Chinese cabbage genome, which is T/G polymorphic. By means of QTL positioning of a Chinese cabbageF2 group, a primary effect QTL tightly linked to low-temperature yellowing of the Chinese cabbage is obtained, the SNP marker tightly linked to low-temperature yellowing of the Chinese cabbage is developed in the region, and the accuracy of the marker can reach 95.9% by verifying 1881 single plants of the F2 group. The marker can be completely used for low-temperature-resistant molecular marker assistant breeding of Chinese cabbage. The molecular marker provided by the invention is low in cost, high in throughput and high in specificity in actual applications, thereby providing an efficient assistant breeding method and technology for accelerating low-temperature-resistant breeding of Chinese cabbage.

This invention discloses a preparation method for the chip of testing polymorphism of PPAR-alpha and PPAR-gamma genes and its usage, in which, probes are set on the carrier. Advantage: realizing the test to the polymorphism of PPAR-alpha gene leu 162val and val 227ala and PPAR-gamma gene pro12 ala to be helpful to the selection of patients of the fatty or diabetes disease.

The invention discloses a novel genome editing tool (Lb2Cas12a-RVR) and a construction method and application method thereof. The novel genome editing tool (Lb2Cas12a-RVR) is characterized by comprising coding plasmids pcDNA3.1-hLb2Cas12a-RVR for coding and expressing an Lb2Cas12a-RVR mutein, wherein the DNA sequence of a coding protein is shown as SEQ ID NO.1. The novel reformed genome editing tool and the construction method and application method thereof aim to solve the problems of low activity and low fidelity of specific gene editing at precise sites, contribute to deep research on screening clinical oncotherapy targets, developing medicines, correcting pathogenic mutation and the like, have genome editing activity in human cells and have better PAM flexibility, high fidelity and special cutting manners.

The invention discloses a method for preparing and applying a QUEFTS model based regionalized facility eggplant special fertilizer, and provides a light, simple, quantitative and reasonable method for preparing and applying the QUEFTS model based regionalized facility eggplant special fertilizer. According to the method, the growth environment and soil fertility condition of eggplant crops are evaluated through key parameters such as yield, yield response and relative yield of eggplants in a complex eggplant fertilization system, and the nutrient demand proportion of nitrogen (N), phosphorus (P2O5) and potassium (K2O) of the special fertilizer for the facility eggplants and the corresponding recommended fertilization method are rapidly and accurately provided. The method is a chemical fertilizer production and application reduction technology which effectively improves the yield of eggplant crops and the utilization rate of the fertilizer, reduces environmental pollution, improves soil fertility, is efficient and easy to implement, and is widely applicable to environmental protection; technical guarantee is provided for industrialized production of the BB fertilizer special for the facility eggplant and/or the compound fertilizer special for the facility eggplant and/or the compound fertilizer with regional characteristics.

The invention discloses optical tweezers for capturing magnetic particles and a preparation method of the optical tweezers. The preparation method comprises the following steps: grinding a magnetic metal into powder, cleaning with ethanol, water and acetone in sequence, and carrying out vacuum drying for 2-8 hours; dispersing the metal powder and tetraethoxysilane into a mixed solvent of ethanol and water in a volume ratio ranging from (1:10) (10:1); after 2-30 minutes of dispersion, putting an ethanol solution of ammonia water into the system, wherein the ethanol solution of the ammonia watercomprises ammonia water and ethanol of 3-15% by mass; carrying out a reaction for 1-12 hours at the room temperature, separating the product by using a magnet, washing with ethanol, water and acetonein sequence, and carrying out vacuum drying for 1-12 hours at 36-100 DEG C so as to obtain a product; carrying out ultrasonic and mechanical stirring and dispersion on the obtained product into methylbenzene. The nanoparticles prepared by using the preparation method have excellent properties of magnetism, bioactivity and fluorescence, and have wide application prospects in biological medicine fields of life sciences, genetics, pharmacology and cell imaging.

The invention discloses a method for preparing and applying a special fertilizer for regional facility tomatoes based on a QUEFTS model. The method for preparing and applying a special fertilizer for facility tomatoes is light, simple, quantitative and reasonable and has a regionalization characteristic. In a complex tomato fertilization system, growth environment and soil fertility of tomatoes are evaluated through key parameters such as available yield, yield response and relative yield of tomatoes. A nutrient demand proportion of nitrogen (N), phosphorus (P2O5) and potassium (K2O) of the special fertilizer for facility tomatoes and a corresponding recommended fertilization method are rapidly and accurately provided. The method is a chemical fertilizer production and application reduction technology, can effectively improve yield of tomato crops and a utilization rate of a fertilizer, reduces environmental pollution, improves soil fertility, is efficient, easy to implement and widely applicable, can protect the environment, and provides a technical guarantee for industrialized production of a specific bulk blend (BB) fertilizer and/or a compound fertilizer and/or a compound blend fertilizer special for facility tomatoes with regional characteristics.

The invention relates to the technical field of breeding, and discloses verticillium wilt resistance-related molecular markers coming from sea island cotton sea 1, and application of the molecular markers. The molecular markers are respectively CER0086, NAU3405 and NAU2026. The molecular markers provided by the invention can help to overcome the defects of the existing breeding technique in the identification of verticillium wilt resistance, and can improve the selection efficiency of the verticillium wilt resistance, thus accelerating the cultivation process of new varieties with resistance to verticillium wilt.

The invention discloses to a method of screening mutants being insensitive to strigolactone. The method includes the steps of: 1) processing of wile-type arabidopsis thaliana seeds: A) weighing seeds and washing the seeds in distilled water; B) adding ethylmethane sulfonate and uniformly mixing the components in a fume cupboard; C) pouring processed ethylmethane sulfonate in NaOH and cleaning the seeds; D) placing the cleaned seeds in an agar solution and moving the seeds into soil by means of a pipette gun for growth; 2) screening of the mutants being insensitive to strigolactone: A) when the seeds are matured, harvesting the seeds and placing the seeds in a centrifugal tube, and disinfecting the seeds with a bleaching agent solution and placing the seeds in a refrigerator; B) sowing the seeds on an AT culture medium containing the strigolactone by means of the pipette gun; C) irradiating the culture dish, and placing the culture dish in a dark place so that the seeds are germinated; and D) selecting a long-root-system arabidopsis thaliana mutant single plant and transplanting the single plant in soil. The method has simple operations and short screening period, and can quickly and high-effectively screen the arabidopsis thaliana mutants being insensitive to the strigolactone.

The invention provides a polyclonal antibody capable of specifically recognizing AAV9 capsid protein and a preparation method thereof, eight AAV9 capsid variable region amino acid sequences are sequentially connected to obtain an amino acid sequence SEQ ID NO.1 of AAV9 variable region VR, a DNA sequence such as SEQ ID NO.2 for coding the AAV9 capsid variable region is obtained from the amino acid sequence, the DNA sequence is inserted into a prokaryotic expression vector pET30a to obtain a plasmid pET30a-AAV-VR, and the plasmid pET30a-AAV-VR is subjected to expression by virtue of a prokaryotic expression vector pET30a to obtain the polyclonal antibody capable of specifically recognizing AAV9 capsid protein. The invention relates to application of an AAV9 variable region protein immune Japanese white rabbit in preparing a polyclonal antibody, detecting antibody titer by enzyme-linked immunosorbent assay (ELISA), Western-blot and cell immunofluorescence detection antibody, and particularly relates to application of the AAV9 variable region protein immune Japanese white rabbit in preparing the polyclonal antibody, detecting antibody titer by enzyme-linked immunosorbent assay (ELISA), and detecting antibody titer by using a cell immunofluorescence method. The titer of the antibody is 1: 10240000, and the antibody can specifically recognize an AAV9 capsid protein sequence. And a foundation is laid for subsequent AAV vector development and AAV biological function research.

The invention relates to the technical field of molecular breeding, particularly Gossypium-barbadense-Hai-1-derived molecular markers related to fiber Micronaire value and application thereof. The molecular markers are BNL3145330 and HAU0764220. The molecular markers are beneficial to overcoming the defects in the fiber quality identification in the existing breeding technique, and can enhance the fiber Micronaire value selection efficiency and accelerate the culturing progress of high-quality new species.

The invention discloses an air conditioner test monitoring device, method and equipment. The device comprises a connecting unit and a control unit; the connecting unit is used for connecting with an air conditioner to be tested, so that the air conditioner to be tested is controlled through the control unit; the control unit is used for controlling the air conditioner to be tested to operate according to preset test conditions; acquiring parameters, to be tested, of the air conditioner to be tested when the air conditioner to be tested runs under the test conditions; judging whether the numerical value of the measured parameter is within a preset range or not; and if not, outputting alarm information. In the above test process, manual operation is reduced, so that personal errors are reduced; and the test process is performed according to the preset conditions, so that the influence of different test environments and operation conditions on the parameters is reduced. Since adverse effects in the test process are reduced, the preset parameter range can be set more accurately, and it is not necessary to set a wide parameter range for containing errors caused by the adverse effects; and the problems of the air conditioners can be found out more easily, and the testing accuracy and efficiency are improved.

The invention discloses a corrosion inhibitor evaluation method for a deepwater natural gas pipeline under a high gas phase flow rate working condition. The method comprises the following steps: investigating a service environment of the deepwater natural gas pipeline; calculating the shearing force of the fluid on the inner wall surface of the submarine pipeline; selecting a corrosion inhibition efficiency evaluation test method; calculating test parameters of the corrosion inhibition efficiency evaluation test method; performing multiple groups of corrosion inhibitor evaluation experiments under different wall surface shear forces and different corrosion inhibitor filling concentrations; drawing a corrosion inhibitor critical filling concentration map; and determining the filling concentration of the corrosion inhibitor under different working conditions. The method for evaluating the corrosion inhibitor of the deepwater natural gas pipeline under the working condition of the high gas phase flow velocity is accurate and effective, scientific and accurate in formulation, beneficial to screening of the efficient corrosion inhibitor and determination of the accurate, economical and safe corrosion inhibitor filling concentration, has important significance, and is one of important links for deepwater oil and gas development and safe conveying guarantee.

The invention relates to a method for quickly detecting koumine in animals. The method includes steps of (1), acquiring body fluid of the poisoned animals and preparing to-be-detected sample fluid; (2), carrying out impurity removal and purification on the to-be-detected sample fluid, obtained at the step (1), by deproteinizing agents and then carrying out determination by the aid of high-performance liquid chromatography-mass spectrometry. The method for quickly detecting the koumine in the animals has the advantages that the method is simple and feasible, the koumine can be detected by the aid of the high-performance liquid chromatography-mass spectrometry, and operation can be facilitated; the method and HRMS (high-resolution mass spectrometer) analysis are combined with each other, accordingly, the content of the koumine in the animals can be quickly and accurately detected, and the method is beneficial to screening and evaluating therapeutic medicines in later periods.

The invention relates to a method for improving activity of a gene knockout and base editing system by using a small molecule compound Rociinostat or Nexturastat A. When a gene editing system is used for gene editing, the small molecule compound Rociinostat or Nexturastat A is added to treat cells, and the Rociinostat or Nexturastat A improves activity of the gene knockout and base editing system in the gene editing system. The method of the invention obtains higher gene editing efficiency, solves a problem of low activity of specific gene editing at precise sites, and is helpful for clinical tumor treatment target screening, drug development, disease-causing mutation correction, animal model preparation, and the like.

The invention relates to a rapid detection method for belladonna alkaloids in an animal. The method comprises the steps that (1) the body fluid of a poisoned animal is taken to prepare a sample liquidto be tested; and (2) after the sample liquid to be tested in the step (1) is impurity-removed and purified with a deproteinizing agent, high-performance liquid chromatography-mass spectrometry is used for determination. The method is simple and practicable. Through the detection of high-performance liquid chromatography-mass spectrometry, the operation is convenient. Combined with HRMS analysis,the content of belladonna alkaloids in the animal can be quickly and accurately detected, which is helpful for screening and evaluation of therapeutic drugs in the later stage.

The invention provides a method for screening a virus broad-spectrum neutral antibody and application thereof. The method comprises the following steps: preparing or providing a virus antigen protein; marking the virus antigen protein by using a fluorescence marker; preparing a cDNA (complementary deoxyribonucleic acid) template of a virus positive patient, designing primers, and amplifying the antibody gene; establishing a yeast antibody display library by using the obtained antibody gene; carrying out antigen protein mixed incubation on yeast cells in the obtained antibody display library and the obtained virus with the fluorescence marker, setting a cell separation door, and separating fluorescence positive yeast cells by a flow cytometry; and carrying out sequencing identification on the fluorescence positive yeast cells to obtain the virus broad-spectrum neutral antibody. The invention also provides a method for establishing an HIV (human immunodeficiency virus) virus antibody yeast display library and application thereof.

The invention provides a device for rapidly separating monospora strains of rice blast germs. The device comprises an operation platform, a driving tank, a teasing needle and a strain bearing platform, wherein a light hole is formed in the operation platform, a driving system is arranged in the driving tank, the strain bearing platform is arranged along the circumferential direction of the light hole and is used for bearing a to-be-operated rice straw, the teasing needle is driven by the driving system to move along the horizontal and vertical directions, the tip of the teasing needle is in contact with the rice straw.