Molecular markers from sea island cotton Hai 1 and related to cotton fiber strength and application of molecular markers

A technology of molecular markers and cotton fibers, which is applied in application, microbiological measurement/inspection, DNA/RNA fragments, etc., can solve the problems of high cost, long cycle, low efficiency, etc., and achieve cost saving, fiber quality improvement, and target selection clear effect

Inactive Publication Date: 2014-01-08
河南永安纺织有限公司
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AI Technical Summary

Problems solved by technology

[0009] In order to overcome many shortcomings such as poor accuracy of phenotypic selection, low efficiency, long cycle and high cost in traditional breeding, and solve the problem of slow progress in fiber quality breeding

Method used

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  • Molecular markers from sea island cotton Hai 1 and related to cotton fiber strength and application of molecular markers
  • Molecular markers from sea island cotton Hai 1 and related to cotton fiber strength and application of molecular markers
  • Molecular markers from sea island cotton Hai 1 and related to cotton fiber strength and application of molecular markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1 Screening Molecular Markers

[0066] (1) Construction of chromosome segment substitution lines and acquisition of phenotypic data

[0067] Using Haidao Mianhai 1 as the donor parent and Zhongmiansuo 45 and Zhongmiansuo 36 as the recurrent parents, two sets of chromosome segment substitution line populations of advanced backcross land-sea hybrids were constructed. The reincarnated parent China Cotton Research Institute 36 (CCRI36) is an early-maturing and excellent upland cotton promotion variety (National Approval Cotton 990007) cultivated by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences. A medium-maturing variety with high yield, lodging resistance and Verticillium wilt resistance was bred, and the donor parent Hai1 (Hai1) was a sea-island cotton cultivar with a dominant aglandular gene and high resistance to Verticillium wilt.

[0068] In the summer of 2003, in Anyang, Henan Province, F 1 , in the winter of the same year in ...

Embodiment 2

[0110] Example 2 Molecular marker selection method for improving fiber strength traits of upland cotton

[0111] Using the molecular marker HAU1980 obtained in Example 1 240 、NAU3177 160 、CGR5149 280 、NAU3405 220 and NAU1325 210 Molecular marker selection is carried out in the breeding population related to Sea Island Cotton Sea 1, etc., including the following steps:

[0112] (1) DNA extraction: take Haidao Mianhai 1 as the donor parent, and upland cotton varieties or strains (such as China Cotton Institute 60, Lumianyan 28) as the recipient parent, carry out hybridization and backcrossing to obtain segregated populations, or use Haidao Mianhai 1 is the donor parent, and the upland cotton varieties (such as Zhongmiansuo 60 and Lumianyan 28) are the introgression lines and their derivatives obtained by the high-generation backcrossing of the recipient parents, or the introgression lines and the land cotton varieties. For the offspring populations of hybridization and back...

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Abstract

The invention relates to the technical field of molecular breeding, in particular to molecular markers from sea island cotton Hai 1 and related to cotton fiber strength and application of the molecular markers. The molecular markers includes HAU1980240, NAU3177160, CGR5149280, NAU3405220 and NAU1325210. By the molecular markers, defects of current breeding techniques in fiber quality identification are overcome, fiber strength selection efficiency can be increased, and breeding process of new varieties of high-quality fibers can be accelerated.

Description

technical field [0001] The invention relates to the technical field of cotton molecular breeding, in particular to a molecular marker related to cotton fiber strength from Sea Island Cotton Sea 1 and its application. Background technique [0002] Cotton is an important economic crop in the world, and cotton fiber is an important raw material for textile industry. Cotton occupies an important position in my country's national economy. With the rapid development of the textile industry and the continuous improvement of people's living standards, the requirements for the quality of cotton fibers are also getting higher and higher. Upland cotton varieties are planted in a large area in my country. Before the 1980s, cotton breeders mainly focused on improving the yield, but did not pay enough attention to the improvement of fiber quality. As a result, most of the cotton varieties in my country showed high yield and high quality. Difference. In the middle and late 1990s, fiber qu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68A01H1/04
Inventor 袁友禄石玉真马留军兰孟焦杨泽茂张金凤张保才李俊文刘爱英王涛龚举武商海红巩万奎
Owner 河南永安纺织有限公司
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