Rapid detection method for belladonna alkaloids in animal

A belladonna alkaloid and detection method technology, applied in the field of food toxin detection, to achieve rapid and accurate detection, convenient operation, simple and easy method

Inactive Publication Date: 2019-04-16
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there are few reports on the detec

Method used

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  • Rapid detection method for belladonna alkaloids in animal
  • Rapid detection method for belladonna alkaloids in animal
  • Rapid detection method for belladonna alkaloids in animal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 2 mL of poisoned mouse urine to prepare the test sample solution, then take 1 mL of the test sample solution and add 4 mL of 1% formic acid acetonitrile solution to remove impurities, purify, and then use high performance liquid chromatography-mass spectrometry for determination. Among them, the high-performance liquid chromatography adopts 3×100mm, 1.9μm C18 chromatographic column; the injection volume is 1μL; the column temperature is 35°C; Gradient elution, wherein, 0-2min, the volume ratio of mobile phase A to mobile phase B is 90:10, 2-5min, the volume ratio of mobile phase A to mobile phase B is 10:90, 5-5.1min, the mobile phase The volume ratio of phase A to mobile phase B is 10:90, 5.1-7min, the volume ratio of mobile phase A to mobile phase B is 90:10, at the 7th minute, the volume ratio of mobile phase A to mobile phase B is 90:10 ; The mass spectrometry conditions: the resolution is 70000, the capillary temperature is 320°C, the auxiliary gas temperature...

Embodiment 2

[0023] Take 3mL of blood from poisoned mice, put the blood in a centrifuge tube rinsed with sodium heparin and mix evenly, and prepare the sample solution to be tested, then take 2mL of the sample solution to be tested and add 5mL of 1% formic acid acetonitrile solution to remove impurities and purify. Then determined by high performance liquid chromatography-mass spectrometry. Among them, the high-performance liquid chromatography adopts 3×100mm, 1.9μm C18 chromatographic column; the injection volume is 1μL; the column temperature is 35°C; Gradient elution, wherein, 0-2min, the volume ratio of mobile phase A to mobile phase B is 90:10, 2-5min, the volume ratio of mobile phase A to mobile phase B is 10:90, 5-5.1min, the mobile phase The volume ratio of phase A to mobile phase B is 10:90, 5.1-7min, the volume ratio of mobile phase A to mobile phase B is 90:10, at the 7th minute, the volume ratio of mobile phase A to mobile phase B is 90:10 ; The mass spectrometry conditions: t...

Embodiment 3

[0025] Take 6mL of the gastric contents of poisoned mice, cut them into pieces in 2mL of 1% formic acid aqueous solution and soak for 30min at room temperature to prepare the sample solution to be tested, then take 3mL of the sample solution to be tested and add 6mL of 1% formic acid in acetonitrile solution to remove impurities and purify, Then determined by high performance liquid chromatography-mass spectrometry. Among them, the high-performance liquid chromatography adopts 3×100mm, 1.9μm C18 chromatographic column; the injection volume is 1μL; the column temperature is 35°C; Gradient elution, wherein, 0-2min, the volume ratio of mobile phase A to mobile phase B is 90:10, 2-5min, the volume ratio of mobile phase A to mobile phase B is 10:90, 5-5.1min, the mobile phase The volume ratio of phase A to mobile phase B is 10:90, 5.1-7min, the volume ratio of mobile phase A to mobile phase B is 90:10, at the 7th minute, the volume ratio of mobile phase A to mobile phase B is 90:10...

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Abstract

The invention relates to a rapid detection method for belladonna alkaloids in an animal. The method comprises the steps that (1) the body fluid of a poisoned animal is taken to prepare a sample liquidto be tested; and (2) after the sample liquid to be tested in the step (1) is impurity-removed and purified with a deproteinizing agent, high-performance liquid chromatography-mass spectrometry is used for determination. The method is simple and practicable. Through the detection of high-performance liquid chromatography-mass spectrometry, the operation is convenient. Combined with HRMS analysis,the content of belladonna alkaloids in the animal can be quickly and accurately detected, which is helpful for screening and evaluation of therapeutic drugs in the later stage.

Description

technical field [0001] The invention relates to a detection method of food toxins, in particular to a rapid detection method of belladonna alkaloids in animals. Background technique [0002] Belladonna is a perennial herbaceous plant with a light brown grayish-brown surface and longitudinal wrinkles. The roots are easy to break, the leaves are shrunken and broken, and the complete leaves are ovate and oval. The surface is yellow-green, the stem is thick and erect, and the upper part is branched. Crown bell-shaped, lavender-brown. Berry spherical, dark purple when ripe. It is black when mature, with long stalks and many seeds. [0003] There are many places where this plant can be cultivated artificially, and it is generally used for sedation, anesthesia, and pain relief. But belladonna is very toxic, even a little bit can cause poisoning, especially to the human nerve center. The main toxic components of belladonna are alkaloids such as hyoscyamine, atropine and scopol...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 罗达龙刘慧妍黄琳李夷君覃蓝
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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