Method for quickly detecting koumine in animals
A technology of keloid and detection method, which is applied in the field of detection of food toxins, to achieve fast and accurate detection and convenient operation
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Embodiment 1
[0024] Take 2 mL of poisoned mouse urine to prepare the test sample solution, then take 1 mL of the test sample solution and add 4 mL of 1% formic acid acetonitrile solution to remove impurities, purify, and then use high performance liquid chromatography-mass spectrometry for determination. Among them, the high-performance liquid chromatography adopts 3×100mm, 1.9μm C18 chromatographic column; the injection volume is 1μL; the column temperature is 35°C; Gradient elution, wherein, 0-2min, the volume ratio of mobile phase A to mobile phase B is 90:10, 2-5min, the volume ratio of mobile phase A to mobile phase B is 10:90, 5-5.1min, the mobile phase The volume ratio of phase A to mobile phase B is 10:90, 5.1-7min, the volume ratio of mobile phase A to mobile phase B is 90:10, at the 7th minute, the volume ratio of mobile phase A to mobile phase B is 90:10 ; The mass spectrometry conditions: the resolution is 70000, the capillary temperature is 320°C, the auxiliary gas temperature...
Embodiment 2
[0026] Take 3mL of blood from poisoned mice, put the blood in a centrifuge tube rinsed with sodium heparin and mix evenly, and prepare the sample solution to be tested, then take 2mL of the sample solution to be tested and add 5mL of 1% formic acid acetonitrile solution to remove impurities and purify. Then determined by high performance liquid chromatography-mass spectrometry. Among them, the high-performance liquid chromatography adopts 3×100mm, 1.9μm C18 chromatographic column; the injection volume is 1μL; the column temperature is 35°C; Gradient elution, wherein, 0-2min, the volume ratio of mobile phase A to mobile phase B is 90:10, 2-5min, the volume ratio of mobile phase A to mobile phase B is 10:90, 5-5.1min, the mobile phase The volume ratio of phase A to mobile phase B is 10:90, 5.1-7min, the volume ratio of mobile phase A to mobile phase B is 90:10, at the 7th minute, the volume ratio of mobile phase A to mobile phase B is 90:10 ; The mass spectrometry conditions: t...
Embodiment 3
[0028] Take 6mL of the gastric contents of poisoned mice, cut them into pieces in 2mL of 1% formic acid aqueous solution and soak for 30min at room temperature to prepare the sample solution to be tested, then take 3mL of the sample solution to be tested and add 6mL of 1% formic acid in acetonitrile solution to remove impurities and purify, Then determined by high performance liquid chromatography-mass spectrometry. Among them, the high-performance liquid chromatography adopts 3×100mm, 1.9μm C18 chromatographic column; the injection volume is 1μL; the column temperature is 35°C; Gradient elution, wherein, 0-2min, the volume ratio of mobile phase A to mobile phase B is 90:10, 2-5min, the volume ratio of mobile phase A to mobile phase B is 10:90, 5-5.1min, the mobile phase The volume ratio of phase A to mobile phase B is 10:90, 5.1-7min, the volume ratio of mobile phase A to mobile phase B is 90:10, at the 7th minute, the volume ratio of mobile phase A to mobile phase B is 90:10...
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