Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for quickly detecting koumine in animals

A technology of keloid and detection method, which is applied in the field of detection of food toxins, to achieve fast and accurate detection and convenient operation

Inactive Publication Date: 2019-04-19
WUZHOU INST FOR FOOD & DRUG CONTROL
View PDF7 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few reports on the detection method of the drug content of hook kisses in animals.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for quickly detecting koumine in animals
  • Method for quickly detecting koumine in animals
  • Method for quickly detecting koumine in animals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take 2 mL of poisoned mouse urine to prepare the test sample solution, then take 1 mL of the test sample solution and add 4 mL of 1% formic acid acetonitrile solution to remove impurities, purify, and then use high performance liquid chromatography-mass spectrometry for determination. Among them, the high-performance liquid chromatography adopts 3×100mm, 1.9μm C18 chromatographic column; the injection volume is 1μL; the column temperature is 35°C; Gradient elution, wherein, 0-2min, the volume ratio of mobile phase A to mobile phase B is 90:10, 2-5min, the volume ratio of mobile phase A to mobile phase B is 10:90, 5-5.1min, the mobile phase The volume ratio of phase A to mobile phase B is 10:90, 5.1-7min, the volume ratio of mobile phase A to mobile phase B is 90:10, at the 7th minute, the volume ratio of mobile phase A to mobile phase B is 90:10 ; The mass spectrometry conditions: the resolution is 70000, the capillary temperature is 320°C, the auxiliary gas temperature...

Embodiment 2

[0026] Take 3mL of blood from poisoned mice, put the blood in a centrifuge tube rinsed with sodium heparin and mix evenly, and prepare the sample solution to be tested, then take 2mL of the sample solution to be tested and add 5mL of 1% formic acid acetonitrile solution to remove impurities and purify. Then determined by high performance liquid chromatography-mass spectrometry. Among them, the high-performance liquid chromatography adopts 3×100mm, 1.9μm C18 chromatographic column; the injection volume is 1μL; the column temperature is 35°C; Gradient elution, wherein, 0-2min, the volume ratio of mobile phase A to mobile phase B is 90:10, 2-5min, the volume ratio of mobile phase A to mobile phase B is 10:90, 5-5.1min, the mobile phase The volume ratio of phase A to mobile phase B is 10:90, 5.1-7min, the volume ratio of mobile phase A to mobile phase B is 90:10, at the 7th minute, the volume ratio of mobile phase A to mobile phase B is 90:10 ; The mass spectrometry conditions: t...

Embodiment 3

[0028] Take 6mL of the gastric contents of poisoned mice, cut them into pieces in 2mL of 1% formic acid aqueous solution and soak for 30min at room temperature to prepare the sample solution to be tested, then take 3mL of the sample solution to be tested and add 6mL of 1% formic acid in acetonitrile solution to remove impurities and purify, Then determined by high performance liquid chromatography-mass spectrometry. Among them, the high-performance liquid chromatography adopts 3×100mm, 1.9μm C18 chromatographic column; the injection volume is 1μL; the column temperature is 35°C; Gradient elution, wherein, 0-2min, the volume ratio of mobile phase A to mobile phase B is 90:10, 2-5min, the volume ratio of mobile phase A to mobile phase B is 10:90, 5-5.1min, the mobile phase The volume ratio of phase A to mobile phase B is 10:90, 5.1-7min, the volume ratio of mobile phase A to mobile phase B is 90:10, at the 7th minute, the volume ratio of mobile phase A to mobile phase B is 90:10...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for quickly detecting koumine in animals. The method includes steps of (1), acquiring body fluid of the poisoned animals and preparing to-be-detected sample fluid; (2), carrying out impurity removal and purification on the to-be-detected sample fluid, obtained at the step (1), by deproteinizing agents and then carrying out determination by the aid of high-performance liquid chromatography-mass spectrometry. The method for quickly detecting the koumine in the animals has the advantages that the method is simple and feasible, the koumine can be detected by the aid of the high-performance liquid chromatography-mass spectrometry, and operation can be facilitated; the method and HRMS (high-resolution mass spectrometer) analysis are combined with each other, accordingly, the content of the koumine in the animals can be quickly and accurately detected, and the method is beneficial to screening and evaluating therapeutic medicines in later periods.

Description

technical field [0001] The invention relates to a method for detecting food toxins, in particular to a method for rapidly detecting kelezin in animals. Background technique [0002] Gelsemium elegans (scientific name: Gelsemium elegans (Gardn. & Champ.) Benth.), is an evergreen woody vine of the genus Gelsemium and Gelsemium, with cylindrical branchlets, distributed in Jiangxi, Fujian, Taiwan, Hunan, Guangdong, Hainan, Guangxi, China , Guizhou, Yunnan and other provinces. India, Myanmar, Thailand, Laos, Vietnam, Malaysia and Indonesia are also distributed. It grows in mountainous roadside bushes at an altitude of 500-2000 meters or under sparse forests on moist and fertile hills and hills. Its main functions are to dislodge accumulated toxins, remove blood stasis and relieve pain, kill insects and relieve itching; analgesic, sedative, anti-inflammatory, mydriasis, and anti-tumor; it is mainly used to treat scabies, eczema, scrofula, carbuncle, boils, and traumatic injuries...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 罗达龙刘慧妍黄林杰李夷君覃蓝
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products