The invention discloses a behavioral experimental device and an experimental method for animal auditory association matching. The behavioral experimental device includes a housing, a light shield, a detection component, a flip plate, a sounding component and a control component. There are multiple partitions inside the shell, which divide the space inside the shell into multiple cavities, and each cavity is provided with a partition plate, which divides the cavity into an experimental cavity and a food cavity. The shell is provided with a plurality of hand openings, each hand opening is connected to an experimental cavity, and the partition plate is provided with a feeding port to communicate with the experimental cavity and the food cavity. The plate is used to block the side wall of the housing with multiple hand openings, there are multiple flaps, each flap is set corresponding to a feeding port, and the sounding component is used to emit sound stimulation toward the experimental animals. The behavioral experimental device It can better quantify and analyze the association matching relationship between animal hearing and behavior, so as to more accurately analyze the function of the temporal lobe of the brain region.