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Establishment method of cerebral-palsy-based temporal lobe epilepsy animal model

An epilepsy animal and a technology for establishing a method, applied in the research field of nervous system diseases, can solve problems such as unfavorable prevention and treatment methods, and achieve the effect of simple establishment method and high survival rate

Active Publication Date: 2019-09-24
FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no animal model of cerebral palsy combined with epilepsy for use, so it is very unfavorable to further understand the occurrence and development of cerebral palsy with epilepsy and the prevention and treatment methods

Method used

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  • Establishment method of cerebral-palsy-based temporal lobe epilepsy animal model
  • Establishment method of cerebral-palsy-based temporal lobe epilepsy animal model
  • Establishment method of cerebral-palsy-based temporal lobe epilepsy animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Design and manufacture of embodiment 1 ischemic hypoxic cerebral palsy model

[0035] 1) 7-day-old SD rat young mice (body weight 18-24g) were anesthetized with 10% chloral hydrate (about 350mg / kg) for unilateral common carotid artery ligation, cut off the neck hair, and cut it open with ophthalmic scissors Make an incision of 0.5 cm, and then use ophthalmic forceps to perform blunt dissection to expand the incision to 1.0-1.5 cm and fully expose the trachea and sternocleidomastoid muscle. Use a glass minute needle to separate the right common carotid artery and make its free segment about 5 mm In the experimental group, the upper and lower ends of the free segment were ligated with 4-0 suture and the centrifugal end was cut off. In the sham operation group, only the right common carotid artery was stretched with glass points, and the right common carotid artery was not ligated, and finally sutured. Make an incision and sterilize the incision.

[0036] 2) Hypoxia treat...

Embodiment 2

[0039] Design and manufacture of embodiment 2 temporal lobe epilepsy model

[0040] 1. Construction process of temporal lobe epilepsy model

[0041] (1) Take the prepared SD rats, inject lithium chloride 127mg / kg intraperitoneally, put them back into the mouse cage for feeding, and the survival rate is 100%; take the SD rats that have been raised for 18 hours, inject scopolamine 10mg / kg intraperitoneally, and inject 30min Afterwards, 10 mg / kg of pilocarpine was injected intraperitoneally, and 10 mg / kg of pilocarpine was given to the rats that did not induce epilepsy 30 minutes after the injection of pilocarpine and induced Racine standard level 4 and above, and so on until successful Epilepsy of grade 4 or above was induced, repeated 4 times (that is, the total injection amount of pilocarpine was 40 mg / kg), and the induction was not successful when the total injection amount of pilocarpine was 60 mg, no more pilocarpine was added dosage and excluded from the group.

[0042] ...

Embodiment 3

[0056] Example 3 The impact of epileptic seizure time on the mortality of temporal lobe epilepsy animal models based on cerebral palsy

[0057] (1) Get 7-day-old SD rat pups (body weight 18-24g), obtain ischemic-hypoxic cerebral palsy rats according to the method in Example 1, send them back to pregnant mice, and raise them for 20 days;

[0058] (2) For the ischemia-hypoxic cerebral palsy rats (i.e. 27-day-old SD rats) fed for 19 days, the temporal lobe epilepsy animal model on the basis of cerebral palsy was induced according to the method of Example 2, wherein, in step (4) Among them, the rats whose epilepsy was successfully induced were divided into 3 groups (5 rats in each group) under strict monitoring for 60 min, 70 min and 80 min respectively, and the mortality rate at each stage was detected, as shown in Table 1.

[0059] Table 1

[0060]

[0061] From the results in Table 1, it can be seen that when the seizure time of the experimental rats in the post-cerebral pa...

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Abstract

The invention provides a cerebral-palsy-based temporal lobe epilepsy animal model and a preparation method thereof. The method includes the steps that after a single-side-neck total arterial ligation operation is carried out on a young animal and hypoxia is conducted for 2-3.5 hours, the animal returns to a female mouse and bred for 3-5 w, pilocarpine is injected into the abdominal cavity of the survival animal to induce a Racine standard epilepsy of the fourth stage or above, and the cerebral-palsy-based temporal lobe epilepsy animal model is obtained. The animal model is simple in establishment method and high in survival rate, the survival rate is 70% or above, and the model animal is damaged in learning and long-term memory function and is free of damage of short-term memory. Electroencephalograms can be used for clinic attack epilepsies besides finding abnormal situations of the electric attack, namely the brain waves, without clinical attack, can be widely applied to treatment of curative effects of different treatment methods on cerebral palsy child patients with the temporal lobe epilepsy, and especially can be used for evaluating the curative effects of temporal lobe epilepsy child patients with cerebral palsy which causes cognitive impairment.

Description

technical field [0001] The invention belongs to the research field of nervous system diseases, in particular to a modeling method for a cerebral palsy disease model combined with temporal lobe epilepsy. Background technique [0002] Cerebral palsy refers to a group of diseases of the central nervous system caused by congenital or perinatal periods caused by a variety of different reasons, mainly non-progressive movement disorders, mainly manifested as central movement disorders, abnormal muscle tone, posture And reflex abnormalities, and may be accompanied by epilepsy, mental retardation, language barriers, visual and hearing impairments and other complications. The incidence of this disease is relatively high. The incidence rate of cerebral palsy in the world is 1‰-5‰, and the incidence rate of children with cerebral palsy in my country is 1.8‰-4‰. The clinical manifestations of cerebral palsy are various. The nature can be divided into spasticity, athetosis type, tetanic t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 刘晶张宏云邹伟马静云
Owner FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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