62 results about "Perinatal periods" patented technology

Porcine circovirus-2 (PCV-2) is a recently identified agent wherein the potential spectrum of PCV-2-associated disease has been expanded by evidence of vertical and sexual transmission and associated reproductive failure in swine populations. PCV-2 was isolated from a litter of aborted piglets from a farm experiencing late term abortions and stillbirths. Severe, diffuse myocarditis was present in one piglet associated with extensive immunohistochemical staining for PCV-2 antigen. Variable amounts of PCV-2 antigen were also present in liver, lung and kidney of multiple fetuses. Inoculation of female pigs with a composition including an immunogen from PCV-2 or an epitope of interest from such an immunogen or with a vector expressing such an immunogen or epitope of interest prior to breeding, such as within the first five weeks of life, or prior to the perinatal period, or repeatedly over a lifetime, or during pregnancy, such as between the 6th and 8th and/or the 10th and 13th weeks of gestation, can prevent myocarditis, abortion and intrauterine infection associated with porcine circovirus-2. In addition, innoculation of male and/or female pigs with the aforementioned compositions can be carried out to prevent transmission of PCV-2 from male to female (or vice versa) during mating. Thus, the invention involves methods and compositions for preventing myocarditis, abortion and intrauterine infection associated with porcine circovirus-2.

Porcine circovirus-2 (PCV-2) is a recently identified agent wherein the potential spectrum of PCV-2-associated disease has been expanded by evidence of vertical and sexual transmission and associated reproductive failure in swine populations. PCV-2 was isolated from a litter of aborted piglets from a farm experiencing late term abortions and stillbirths. Severe, diffuse myocarditis was present in one piglet associated with extensive immunohistochemical staining for PCV-2 antigen. Variable amounts of PCV-2 antigen were also present in liver, lung and kidney of multiple fetuses. Inoculation of female pigs with a composition including an immunogen from PCV-2 or an epitope of interest from such an immunogen or with a vector expressing such an immunogen or epitope of interest prior to breeding, such as within the first five weeks of life, or prior to the perinatal period, or repeatedly over a lifetime, or during pregnancy, such as between the 6and 8and/or the 10and 13weeks of gestation, can lower viral titer, prevent myocarditis, abortion and intrauterine infection associated with porcine circovirus-2. In addition, innoculation of male and/or female pigs with the aforementioned compositions can be carried out to prevent transmission of PCV-2 from male to female (or vice versa) during mating. Thus, the invention involves methods and compositions for preventing myocarditis, abortion and intrauterine infection associated with porcine circovirus-2.

The invention relates to a diagnosing reagent for hepatitis B, disclosing detection grains for e antigens of the hepatitis B virus, which are of the luminous grains coated by anti-HBE antibodies. The invention also discloses preparation and application for the detection grains for e antigens of the hepatitis B virus; moreover, the invention further discloses an outside-body diagnosis reagent box for detecting e antigens of the hepatitis B in a blood serum sample of human beings as well as a method for utilizing the light excitation chemiluminescence principle to quantitatively and qualitatively detect e antigens of the hepatitis B virus. The reagent box of the invention can be jointly used to diagnose the individual acute or chronic hepatitis B together with other blood serums and clinic information, and screen the hepatitis B for women in the perinatal period so as to judge the risk of newborn babies contaminating the hepatitis B.

The invention relates to hepatitis B diagnosis reagent, which discloses surface antibody testing fine particles for hepatitis B, which are luminous fine particles for coating surface antibody of hepatitis B. The invention also discloses a preparation and an application for the surface antibody testing fine particles for hepatitis B. In addition, the invention further discloses an in vitro diagnosis kit for measuring the surface antibody of hepatitis B and simultaneously discloses a method for using the kit. The kit can be used jointly with other blood serum and clinical information to diagnose the condition of acute or chronic hepatitis B infection of an individual, and can also be used for sieving hepatitis B of females in the perinatal period, so as to judge the danger of hepatitis B infection of newly born babies.

A pre-mixed forage for the milk cow in early perinatal period is prepared from glucose oxidase or yeast protein powder, ammonium chloride, magnesium sulfate, calcium sulfate, urea phosphate, distiller' s grains, and the mixture of copper sulfate, ferrous sulfate, manganese sulfate, Zinc sulfate, magnesium sulfate, cobalt chloride, potassium iodate, sodium selenite, VA, VD3, VE, calcium hydrogen phosphate and calcium carbonate. Its preparing process is also disclosed.

The invention discloses feed additive and a production method thereof. The invention belongs to the field of feed additive, especially relates to the product of cow feed additive. The feed comprises the following components by weight in percent: 30-50 wt% of extruded soybean, 4-9 wt% of urea, 3-8 wt% of yeast protein, 20-30 wt% of gloeostereum insarnatum, 15-25 wt% of ganoderma lucidum, 2-9 wt% of slow-releasing agent, and 3-6 wt% of aspergillus awamori culture. The feed production method provided by the invention provides fully nutrient storage for the cows at the perinatal period, improves the daily milk production and peak sustainable power, and improves the pregnancy rate of the cows.

The invention provides glycerol-producing saccharomyces cerevisiae NAU-ZH-GY1 and application thereof, belonging to the field of microbial fermentation. The fungus is separated from unprocessed honey and is identified as saccharomyces cerevisiae with a collection number of CGMCC (China General Microbiological Culture Collection Center) No. 4551. The strain is obtained through liquid fermentation; fermentative culture comprises abundant glycerol, a large amount of living saccharomyces and rich microbial metabolism substances such as amino acid, vitamins and the like; the saccharomyces cerevisiae is applied to producing a feedstuff additive at the perinatal period of a ruminant; the glycerol can be used as an energy supplement to relieve negative energy balance; by means of the saccharomyces cerevisiae and the microbial metabolism substances such as amino acid, vitamins and the like, animal gastrointestinal tract flora can be adjusted, pathogenic microorganisms can be antagonized, immunity of organisms can be improved, conversion and absorption of nutritive materials can be promoted, and production performance can be improved; and the glycerol-producing saccharomyces cerevisiae NAU-ZH-GY1 is a novel environmentally-friendly, nutritive and safe feedstuff additive with high cost performance.

The invention discloses a gelatinized anionic salt additive special for cows at early perinatal period and a preparation method thereof. The additive is composed of the following components by weight: 35-40 parts of corn flour, 0.5-1.0 part of sucrose, 15-20 parts of calcium chloride, 10-15 parts of calcium sulfate, 15-20 parts of ammonium chloride, 3-5 parts of ammonium sulfate, 0.5-1 part of magnesium oxide, and 8-10 parts of rumen-protected choline, wherein the calcium chloride, calcium sulfate, ammonium chloride, ammonium sulfate and magnesium oxide are gelatinized by corn flour and are then coated. Without affecting or lowering the dry matter intake of cows, the gelatinized anionic salt additive provided by the invention can effectively control postnatal hypocalcemia and other metabolic diseases caused by the content decrease of blood calcium in blood after cow labor onset and lactation, improve lipid metabolism, strengthen immunity, and relieve negative energy balance.

The invention discloses a kit for screening high-quality blastulas. According to the kit, birth safety is forecasted before a human assisted reproduction blastula is implanted, and the kit comprises products of expression quantities of 398 genes as shown in a table 2 in wall trophoblast cells and polar trophoblast cells. The human assisted reproduction blastula is normally developed, and a pregnant woman normally delivers if the expression quantity of each gene in the 398 genes of the wall trophoblast cells of the human assisted reproduction blastula (a blastula implanted before fifth days of in-vitro fertilization) is twice or more or half or less that of the same gene in the 398 genes of the polar trophoblast cells. The kit has important application values for scientific judgment of transplantation strategies and decisions and possibility reduction of perinatal period complications of pregnant women and sick children in assisted reproduction.

The invention discloses a feed additive for reducing cow body cells and treating recessive mastitis and a preparation method of the feed additive. The feed additive is prepared from the following raw materials in parts by weight: 10-15 parts of antibacterial peptide, 5-10 parts of geobacillus sp, 10-15 parts of yeast, 15-20 parts of cottonrose hibiscus leaf, 20-30 parts of andrographis paniculata, 10-20 parts of thesium chinense, 5-10 parts of cowherb seed, 15-20 parts of mongolian dandelion herb, 10-20 parts of epimedium, 20-30 parts of astragalus membranaceus, 5-10 parts of lindera aggregata leaf, 1-5 parts of sea buckthorn seed powder, 1-5 parts of corn pollen, 0.2-0.3 part of organic chelated zinc, 0.3-1 part of organic chromium and 8-15 parts of coated threonine. The additive can quickly reduce the body cell number, quickly cure the recessive mastitis, enable a cow to quickly restore to be the healthy production state, avoid generation of clinical mastitis, comprehensively improve the immunologic function of the body of the cow in the lactation period, effectively prevent generation of various diseases, highly improve the anti-stress capability of the cow in the perinatal period, effectively prevent generation of antenatal and puerperal diseases, highly improve the vaccine protection rate, strengthen the immune antibody level, and avoid immune clearance, immune inhibition and vaccine stress.

The invention provides a feed for Xiang pigs at a perinatal period and a preparation method of the feed. The feed comprises the following raw materials in parts by weight: 40-60 parts of corn flour, 30-50 parts of wheat bran, 20-40 parts of bean cakes, 10-25 parts of soybean flour, 5-15 parts of rice bran, 10-25 parts of distillers' grains, 5-20 parts of motherwort, 5-15 parts of shrimp powder, 5-15 parts of fish meal, 5-15 parts of red jujube powder, 5-10 parts of brown sugar, 10-30 parts of probiotics powder, 0.5-2.5 parts of sodium selenite, 1-3 parts of zinc sulfate, 0.5-1.5 parts of potassium iodide, 1-5 parts of tryptophan, 0.5-1.5 parts of dicalcium phosphate and 0.5-1.5 parts of manganese carbonate. Various raw materials are in a reasonable compounding ratio, so that the nutritionof the feed for Xiang pigs at the perinatal period provided by the invention can meet various nutrition requirements of the Xiang pigs within the perinatal period, prenatal milk secretion and postpartum milk secretion are facilitated, the health of intestinal tracts is strengthened, prenatal and pospartum constipation situations are eliminated, postpartum inflammation is eliminated, and the appetite of the Xiang pigs can be promoted.

The invention relates to hepatitis B diagnosis reagent, which discloses core antibody testing fine particles for hepatitis B, which are luminous fine particles for coating core antibody of hepatitis B. The invention also discloses a preparation and an application for the core antibody testing fine particles for hepatitis B. In addition, the invention further discloses an in vitro diagnosis kit for measuring the core antibody of hepatitis B and simultaneously discloses a method for using the kit. The kit can be used jointly with other blood serum and clinical information to diagnose the condition of acute or chronic hepatitis B infection of an individual, and can also be used for sieving hepatitis B of females in the perinatal period, so as to judge the danger of hepatitis B infection of newly born babies.

The invention provides saline and alkaline land golden wolfberry planting technology comprising cutting seedling technology, transplanting technology, water and fertilizer management technology, pest control technology and fresh fruit harvesting technology. By application of the planting technology provided by the invention, the thousand seed weight of the obtained golden wolfberry fresh fruits reaches 130 grams, 24.5% of soluble solids are contained, the leaf-fruit ratio is 4.8:1.0, the branching rate is 17.82% the fresh fruits of yield per mu is higher than 300kg yearly, 1200 kg of fresh fruits can be produced in perinatal period (two years), and 300 kg of dry fruits can be obtained. The saline and alkaline land golden wolfberry planting technology has a wide artificial cultivation prospect and has been applied to large-scale planting practice, is high in yield and is obvious in economic benefits; no pesticide is sprayed in the planting process, the obtained golden wolfberry is not polluted by any pesticide, thereby being green and healthy and satisfying the consumption concept and health consciousness of the current consumers.

The invention relates to the cow culturing field, and discloses a method for raising a cow's milking function, and the method comprises the steps of selecting species of a cow; limiting culturing density; guaranteeing scientific management of the species source and the culturing environment; cleaning the body surface and the inside of the cow; reducing the probability of a tuberculosis and a Brucella to 0.2-0.5%; and controlling the nutrition supply and activity volume of the cow preparing to get pregnant, which means a lot to milk quality. The physiological status of the cow in perinatal period influences not only the milking status of this birth but also has a tight connection to milking in later conceptions, therefore, according to the method, a nutrition and health soup is supplied to the cow after a concentrated feed is supplied, and the milking function of the cow can be raised obviously.

The invention discloses a milk cow energy extender, which is composed of the following components by weight percent: 3-20% of palm oil, 1.1-7.1% of Span 80, 5-10% of tween 80, 0.5-1% of ethanol and the balance of water. The milk cow energy extender is an oil-in-water type palm oil micro emulsion, which is stable and high in digestivity, can be drunk under the condition that the milk cow has poor appetite and the feed intake of the milk cow is decreased, can supplement energy for milk cow, slow milk cow heat stress and improve milk cow production performance by drinking water in summer high-temperature weathers, can also supplement energy and reduce the negative influence of the decrease of feed intake on the milk cow by water drinking in the milk cow perinatal period; the preparation method is simple and convenient for operation; and the used raw materials are easy to obtain and moderate in price.

The invention discloses a method for performing in-vitro culture to bovine preadipocytes, belonging to the technical field of cell and tissue engineering. The method is based on the traditional subculture method, a tissue homogenizer is utilized and combines with the collagenase I culture medium to treat the fatty tissue, a DMEM (Dulbecco's Modified Eagle Medium)/F12 culture solution containing fetal bovine serum is used to perform in-vitro culture to bovine preadipocytes, and a new model of the in-vitro culture of bovine preadipocytes is successfully built. The technology is advanced and simple, the preparation of the culture medium is scientific and reasonable, manpower and material resources are saved, and the treatment time of the test sample is greatly shortened; the obtained cell morphology is best, the RNA (Ribonucleic Acid) culture and extraction efficiency is maximum, the preadipocyte culture method is convenient and efficient in a long term and lays the foundation for the gene expression and protein expression and the signal pathway in the later intensive study of the proliferation and differentiation process of preadipocytes; and a new idea and a new method are provided to regulate body fatness deposition of beef cow and prevent diseases in the perinatal period and lactation peak period of milk cow, etc.

The invention discloses a preparation method of a master cell bank for umbilical cord derived mesenchymal stem cells in a perinatal period. The preparation method comprises the following steps: S1, collecting a newborn umbilical cord in a sterile way; S2, carrying out pathogenic microorganism screening on a lying-in woman, and carrying out sterile detection on an umbilical cord sample; S3, cleaning the umbilical cord sample, sterilizing, and rinsing with normal saline; S4, carrying out infusion on a blood vessel with the normal saline, squeezing out residual blood, cutting up the umbilical cord sample into tissue blocks, and rinsing; S5, dissolving gelatin with deionized water, carrying out moist heat sterilization, cooling, adding 2XDMEM(L), and uniformly mixing; S6, taking the tissue blocks, adding gelatin solution, transferring into a tissue culture flask, staying overnight in a refrigerator at the temperature of 4 DEG C, adding a complete medium, and putting in a CO2 incubator until cells grow all over a gel layer; and S7, harvesting cells by virtue of a trypsin digestion process, filtering by virtue of a cell strainer, and centrifuging to obtain PO generation MSCs, and cryopreserving the PO generation MSCs, namely an umbilical cord derived MSCs master cell bank is prepared.

The invention provides a molecular marker related to cow perinatal period metabolic disease resistance and application. The molecular marker is obtained on the basis of cow whole-genome association analysis development, 10 SNP molecular markers are involved, and upstream and downstream flanking sequences of SNP loci are shown in SEQ ID NO.1-2, SEQ ID NO.3-4,..., and SEQ ID NO.19-20. The molecularmarker can be used for identifying cow metabolism disease resistance genetically, cows with the poor metabolism resistance are eliminated, the cows with the high metabolism disease resistance are cultured, and the effective method is supplied to marker auxiliary selection for cow perinatal period energy metabolism disease genetic resistance.

The invention discloses a biological feed additive for improving the reproductive performance of a herbivorous female animal. The biological feed additive is prepared from (1) a yeast culture or an active dry yeast, (2) rumen protected choline, (3) a lactic acid bacteria preparation, (4) a complex enzyme, (5) glucose oxidase, (6) vitamin E powder, (7) a beta-carotene preparation or carrot residues and (8) a diluent. In fine feed in the perinatal period and the early-lactation period (from 21 days before the due date to 90 days after delivery), by adding the biological feed additive, the dry material feed intake in the later period of the perinatal period of the herbivorous female animal can be obviously improved, and the reproductive performance and health level of the herbivorous female animal are improved.

The invention relates to a complete feed for improving the sow feed intake in the summer perinatal period, and belongs to the technical field of animal feed. The complete feed comprises the following components in parts by weight: 50-70 parts of corn, 10-20 parts of soybean meal, 10-15 parts of extruded soybean, 1-2 parts of glucose, 1-3 parts of deep sea fish oil, 0.40-0.70 part of calcium powder, 0.50-1.50 parts of calcium hydrophosphate, 0.10-0.30 part of sodium chloride, 0.10-0.20 part of potassium chloride, 0.03-0.05 part of a yeast extract, 0.05-0.15 part of xylooligosaccharide and 1 part of a compound premix. The invention further relates to a preparation method for the complete feed. The raw materials in the formula are mixed and pelletized so as to obtain the complete feed. The various macroelements, the yeast extract, xylooligosaccharide and other nutritional components are used in a coordinated manner, nutrition needed by the sow breeding in the perinatal period can be met, and the sow feed intake under summer heat stress is increased, so that high protein and high fat needed by porkets can be supplied, and growth and development of the porkets can be promoted. The complete feed is convenient in raw material formula source and low in cost, and is beneficial to mass popularization.

The invention provides an eight percent premix used in the early perinatal period of dairy cows and a preparation method of the premix. Various organic trace elements are added into the premix, are scientific and reasonable in content and are suitable for the dairy cows in the early perinatal period, and not only is the defect of incomplete absorption of trace elements in the early perinatal period of the dairy cows overcome, but also poisoning of trace elements is eliminated.

The invention provides a nutrient supplement for first-delivery sows, and belongs to the technical field of breeding of first-delivery sows. The nutrient supplement comprises 100-200 parts of poly-isomaltose, 100-200 parts of baking soda, 50-100 parts of probiotics, 60-150 parts of chitosan oligosaccharides, 100-150 parts of L-Lysine monohydrochloride, 50-100 parts of methionine, 50-120 parts of calcium lactate, 0.1-0.3 part of zinc glycinate, 0.08 -0.12 part of manganese glycinate, 0.01-0.03 part of cupric glycinate, 50-100 parts of an acidulant, 150-200 parts of vitamin E, 10-15 parts of vitamin A, 2-3 parts of vitamin D3 and 10-500 parts of silicon dioxide. A preparation method of the nutrient supplement comprises the following steps of weighing the components in parts by weight, sequentially adding the components in parts by weight to a mixing machine in the sequence from the big components to the small components, performing uniform mixing, and performing subpacking with a non-toxic bag. According to the nutrient supplement, the physical development of the first-delivery sows can be promoted, the firstborn stress of the first-delivery sows can be reduced, the body storage of the first-delivery sows can be strengthened, the food consumption of the sows at the early period of a lactation period can be increased, the disease resistance of the first-delivery sows at a perinatal period can be strengthened, the syndromes of the sows for second pregnancy can be alleviated, the service life of the sows can be prolonged, and the rejection rate of the sows can be reduced.

The present invention relates to a lick brick for ruminants and particularly discloses a special compound premixed feed lick brick for dairy cows in a perinatal period. The special compound premixed feed lick brick for the dairy cows in the perinatal period comprises edible salt, cement, bentonite, calcium propionate, bacitracin zinc, baking soda, calcium formate, calcium carbonate, calcium hydrogen phosphate, a flavoring agent, glycine iron, copper glycinate, zinc glycinate, magnesium oxide, manganese glycinate, yeast selenium, cobalt chloride, calcium iodate, rumensin and compound multiple vitamins. A density of the lick brick is 1.7-1.9 g/cm<3>. According to nutritional requirements and licking characteristics of the dairy cows in the perinatal period, the compound premixed feed lick brick suitable for the dairy cows in the perinatal period is scientific in designs and formulas, rich in nutrition, adequate in feed intake and appropriate in hardness, and can effectively improve a production performance of the dairy cows in the perinatal period. Experiments show that a long-term licking of the lick brick can effectively reduce an incidence of trace element deficiency in the dairy cows in the perinatal period and can significantly improve milk production amount and quality.

The invention relates to health-care granules for relieving perinatal period dairy cow energy metabolism disorder and a preparation method thereof, and belongs to the technical field of livestock breeding. The preparation method comprises the following steps: mixing palm oil, corn germ oil and medical stomach-soluble polyacrylic resin No.4 at 90 DEG C and dissolving; and immediately adding soybeanlecithin, zesterol, soy isoflavone, myricetin, nicotinamide, choline, vitamin E, calcium aspartate and magnesium stearate, and performing stirring and mixing for 1 minute, and performing spray granulation on the mixture to obtain the health-care granules for relieving the energy metabolism disorder of the dairy cow in the perinatal period. The health-care granules can prevent postpartum energy metabolic diseases, and is safe, practical and convenient. The health-care granules prepared by the components and the method disclosed by the invention can be used for relieving liver function damage,reducing ketone body generation, reducing the incidence rate of ketosis, relieving diseases caused by negative energy balance and improving the production performance of dairy cows in a perinatal period.