Preparation method of bypass rumen protective glucose
A technology of glucose and rumen passing is applied in the field of preparation of rumen-protected glucose, which can solve the problems of full utilization, inability to ruminants, low rumen passing rate, etc., so as to prevent subclinical ketosis, clinical ketosis and fatty liver disease. , Improve the estrus conception rate, reduce the effect of postpartum weightlessness
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example 1
[0028] Example 1, this example prepares 30% rumen-protected glucose
[0029] Weigh 30.5 kg of glucose, crush it through 120 meshes, weigh 200 mg of high-temperature-resistant lipase with an enzyme activity of 10000U / g, add it to glucose and mix it, add 8 kg of water to make a wet material, extrude it with an extruder and shoot it, Dry at low temperature below 60°C to make glucose pellets;
[0030] Heat 65.5 kg of saturated fatty acid and 4 kg of chitosan to 120°C to melt;
[0031] Put the glucose pellets into the fluidized bed of the bottom spray coating machine for fluidization, and spray the molten material cooled below 100°C onto the surface of the glucose pellets to obtain rumen-passing protected glucose containing 30% glucose.
example 2
[0032] Example 2, this example prepares 50% rumen-protected glucose
[0033] Weigh 50.5 kg of glucose, crush it through 120 meshes, weigh 400 mg of high-temperature-resistant lipase with an enzyme activity of 10000U / g, add it to glucose and mix it, add 13 kg of water to make a wet material, extrude it with an extruder and shoot it, Dry at low temperature below 60°C to make glucose pellets;
[0034] 47.5 kilograms of saturated fatty acids and 2 kilograms of polyacrylic acid resin IV are heated to 120 ° C for melting;
[0035] Put the glucose pellets into the fluidized bed of the bottom spray coating machine for fluidization, and spray the molten material cooled below 100°C on the surface of the glucose pellets to obtain rumen-passing protected glucose containing 50% glucose.
example 3
[0038] Example 3 Effect embodiment
[0039] In order to verify the product performance of the rumen-passing glucose prepared by the present invention, use in vitro method (In Vitro) to simulate the digestive tract of ruminants to carry out stability test (simulated rumen environment: pH value 6.6 buffer solution and pH value 5.4 buffer solution, abomasum and ten Duodenum: pH 2.4 buffer solution) and evaluation.
[0040] Materials and methods
[0041]In this study, pH 6.6, pH 5.4, and pH 2.4 buffers were used to simulate the rumen, abomasum and duodenal digestive tract of ruminants, respectively.
[0042] Formulation of buffer solutions with different pH values
[0043] Table 1: Buffer solution formulations with different pH values (unit: gram)
[0044]
[0045] Dissolve the substances listed in the table in a small amount of distilled water and set the volume to 1000ml.
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