Glycerol-producing saccharomyces cerevisiae NAU-ZH-GY1 and application thereof
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A technology of NAU-ZH-GY1, Saccharomyces cerevisiae, applied in application, fermentation, microorganism and other directions, to achieve the effect of simple production process, correction of energy metabolism disorders, and improvement of production performance
Active Publication Date: 2011-09-07
NANJING AGRICULTURAL UNIVERSITY
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However, there is no report on the use of glycerol produced by fermentation as a fe
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Embodiment 1
[0029] Example 1: Isolation of glycerol-producing strains
[0030] Weigh 10g of the sample (unprocessed honey), put it into a Erlenmeyer flask filled with 90mL of sterile normal saline, shake it for 20min to make a bacterial suspension, draw 0.1mL of the bacterial suspension and inoculate it on the malt juice agar medium, at 28°C Cultivate in the incubator for 24h.
[0031] Yeasts were selected and fermented for glycerol production, respectively, with a shaker at 150 rpm and a temperature of 28° C. for 48 hours. Medium formula (g / L): glucose 200, urea 2, KH2 PO 4 0.4, do not adjust the pH, sterilize at 115°C for 20min.
[0032] A bacterium with high glycerol production was screened and named NAU-ZH-GY1, which was identified as Saccharomyces cerevisiae. It was preserved in the General Microbiology Center of China Committee for Microbial Culture Collection on January 13, 2011. Deposit number CGMCC No.4551.
[0033] Main biological characteristics: observed under a microscope...
Embodiment 2
[0037] Embodiment 2: Utilize glycerol producing strain NAU-ZH-GY1 to produce glycerol by fermentation
[0038] 1) Medium:
[0039] Seed medium (g / L): glucose 200, urea 2, yeast extract 4, without adjusting the pH, sterilized at 115°C for 20 minutes.
[0040] Fermentation medium (g / L): glucose 200, urea 4, corn steep liquor powder 5, sodium chloride 20, magnesium sulfate 0.5, calcium chloride 0.1, adjust pH to 5.0, and sterilize at 115°C for 20 minutes.
[0041] 2) Culture conditions:
[0042] Primary seed culture: For shake flask fermentation, use 500ml Erlenmeyer flask to fill 100mL of seed medium, insert glycerol-producing Saccharomyces cerevisiae NAU-ZH-GY1 into the seed medium, culture at 30°C, 150rpm shaker for 24 hours.
[0043] Secondary seed culture: 10L seed fermentation tank seed medium 6L, inoculum size 5%, 30°C, 150rpm shaker culture for 24 hours.
[0044] Fermentation tank culture: 60L of fermentation medium in 100L fully automatic stirring fermentation tank, f...
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Abstract
The invention provides glycerol-producing saccharomyces cerevisiae NAU-ZH-GY1 and application thereof, belonging to the field of microbial fermentation. The fungus is separated from unprocessed honey and is identified as saccharomyces cerevisiae with a collection number of CGMCC (China General Microbiological Culture Collection Center) No. 4551. The strain is obtained through liquid fermentation; fermentative culture comprises abundant glycerol, a large amount of living saccharomyces and rich microbial metabolism substances such as amino acid, vitamins and the like; the saccharomyces cerevisiae is applied to producing a feedstuff additive at the perinatal period of a ruminant; the glycerol can be used as an energy supplement to relieve negative energy balance; by means of the saccharomyces cerevisiae and the microbial metabolism substances such as amino acid, vitamins and the like, animal gastrointestinal tract flora can be adjusted, pathogenic microorganisms can be antagonized, immunity of organisms can be improved, conversion and absorption of nutritive materials can be promoted, and production performance can be improved; and the glycerol-producing saccharomyces cerevisiae NAU-ZH-GY1 is a novel environmentally-friendly, nutritive and safe feedstuff additive with high cost performance.
Description
technical field [0001] The invention relates to glycerol-producing Saccharomyces cerevisiae NAU-ZH-GY1 and application thereof, which belongs to the field of microbial fermentation and uses microbial fermentation to produce glycerin, and is suitable for the production of ruminant feed additives. Background technique [0002] The perinatal period of ruminants refers to the stage from late pregnancy (2 to 3 weeks before delivery) to early lactation (2 to 3 weeks after delivery), also known as the transition period. Due to the decline in feed intake in late pregnancy, dry matter intake Reduced, can not meet the needs of fetal growth and energy metabolism, and postpartum lactation peak (postpartum 4 ~ 6 weeks) before the peak of dry matter intake (postpartum 8 ~ 10 weeks), so that the body is in a nutritional balance of energy stress state. Studies have shown that the glucose consumed by the fetus in the late pregnancy can account for 46% of the glucose produced by the mother, ...
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