Molecular marker related to cow perinatal period metabolic disease resistance and application

A technology for metabolic diseases and molecular markers, applied in the fields of genetics, molecular biology, and animal breeding, can solve the problems of limited molecular markers and candidate genes

Active Publication Date: 2019-08-09
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have confirmed that there are genetic differences among dairy cows in susceptibility to metabolic diseases during the peripartum period, but the molecular markers and candidate genes related to metabolic diseases are still very limited.

Method used

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  • Molecular marker related to cow perinatal period metabolic disease resistance and application
  • Molecular marker related to cow perinatal period metabolic disease resistance and application
  • Molecular marker related to cow perinatal period metabolic disease resistance and application

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1 Acquisition of molecular markers related to perinatal metabolic diseases of dairy cows

[0027] 1. Data collection and sample collection

[0028] The phenotype data of abomasum displacement and ketosis were collected from 21 cattle farms of Sanyuan Lvhe Dairy Cow Breeding Center in Beijing. Blood samples (3,510 individuals) were selected from 608 diseased dairy cows and 2902 healthy control cows.

[0029] 2. DNA extraction and bovine 54K SNP chip genotype detection

[0030] Cattle blood samples were collected by tail root vein blood sampling method, and blood genome DNA extraction kit (DP318) from Tiangen Biochemical Technology (Beijing) Co., Ltd. was used to extract bovine blood DNA. The DNA sample quality requirement is that the concentration requirement is greater than 50ng / ul, the sample purity requirement is that OD260 / OD280 is between 1.6 and 2.0, and OD260 / OD230 is between 1.8 and 2.1. The DNA samples that passed the quality inspection were genotyped...

Embodiment 2

[0051] Embodiment 2 Verification of the association degree of 10 SNP sites of the present invention

[0052] In this embodiment, the significance of the 10 SNP molecular marker genotypes screened in Example 1 was tested using the chi-square test method. As can be seen from Table 2, there is a certain effect value in all 10 SNP sites, indicating that SNP loci associated with ketosis. According to the positive and negative of the effect value, combined with the phenotype data (0 means no disease, 1 means disease) genotype coding data (0, 1, 2, homozygous minimal allele code is 0), each SNP marker can be judged Relative disease resistance dominant alleles. For example, if the SNP effect value is positive, the smallest allele of the marker is the resistance allele, and if the SNP effect value is negative, the largest allele of the marker is the resistance allele. Further statistical analysis of genotype distributions in diseased individuals and healthy individuals found that the...

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Abstract

The invention provides a molecular marker related to cow perinatal period metabolic disease resistance and application. The molecular marker is obtained on the basis of cow whole-genome association analysis development, 10 SNP molecular markers are involved, and upstream and downstream flanking sequences of SNP loci are shown in SEQ ID NO.1-2, SEQ ID NO.3-4,..., and SEQ ID NO.19-20. The molecularmarker can be used for identifying cow metabolism disease resistance genetically, cows with the poor metabolism resistance are eliminated, the cows with the high metabolism disease resistance are cultured, and the effective method is supplied to marker auxiliary selection for cow perinatal period energy metabolism disease genetic resistance.

Description

technical field [0001] The invention belongs to the technical fields of animal breeding, genetics and molecular biology, and specifically relates to molecular markers and applications related to the resistance of dairy cows to perinatal metabolic diseases. Background technique [0002] The peripartum period is a critical stage in the production of dairy cows. Negative energy balance is the main metabolic feature of this period, leading to a high incidence of metabolic diseases. Ketosis and abomasum displacement are two common metabolic diseases in peripartum dairy cows, especially in high-yielding cows, which seriously affect the health performance and production life of dairy cows. Ketosis is a perinatal energy metabolism disorder caused by energy metabolism disorder, with hepatic fatty acid oxidation metabolism disorder and ketone body (BHBA, acetoacetate, acetone, etc.) increase as the main pathological features. Hyperlipidemia and other characteristics, the incidence ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 张毅黄河天张胜利
Owner CHINA AGRI UNIV
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