Kit for screening high-quality blastulas
A kit and blastocyst technology, applied in the field of biomedicine, can solve problems such as uncertainty of assisted reproductive technology, risk of pregnancy outcome of assisted reproductive technology, increased health risks of babies born, social economy and family burden, etc.
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[0020] The following examples facilitate a better understanding of the present invention, but do not limit the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent stores. Quantitative experiments in the following examples were all set up to repeat the experiments three times, and the results were averaged.
[0021] The research content and specimen collection were approved by the Scientific Research Ethics Committee of Beijing Jishuitan Hospital (Medical Ethics Review: 2014075).
[0022] The Illumina HiSeq2000 system is a product of Shanghai Renke Biotechnology Co., Ltd. 1×Expand High Fidelity PCR Buffer is a product of Roche, the product catalog number is 11732641001.
[0023] Whole cell lysis buffer: pH 7.5, 50 mmol / L Tris-HCl buffer solution containing 0.1% (0.1 mg / 100 mL...
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