Kit for screening high-quality blastulas

A kit and blastocyst technology, applied in the field of biomedicine, can solve problems such as uncertainty of assisted reproductive technology, risk of pregnancy outcome of assisted reproductive technology, increased health risks of babies born, social economy and family burden, etc.

Inactive Publication Date: 2017-12-01
BEIJING JISHUITAN HOSPITAL
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Problems solved by technology

However, compared with natural pregnancy, epidemiological and clinical studies have shown that, excluding the influence of infertility disease factors, assisted reproductive technology itself has uncertain hidden d

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  • Kit for screening high-quality blastulas
  • Kit for screening high-quality blastulas
  • Kit for screening high-quality blastulas

Examples

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Embodiment Construction

[0020] The following examples facilitate a better understanding of the present invention, but do not limit the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent stores. Quantitative experiments in the following examples were all set up to repeat the experiments three times, and the results were averaged.

[0021] The research content and specimen collection were approved by the Scientific Research Ethics Committee of Beijing Jishuitan Hospital (Medical Ethics Review: 2014075).

[0022] The Illumina HiSeq2000 system is a product of Shanghai Renke Biotechnology Co., Ltd. 1×Expand High Fidelity PCR Buffer is a product of Roche, the product catalog number is 11732641001.

[0023] Whole cell lysis buffer: pH 7.5, 50 mmol / L Tris-HCl buffer solution containing 0.1% (0.1 mg / 100 mL...

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Abstract

The invention discloses a kit for screening high-quality blastulas. According to the kit, birth safety is forecasted before a human assisted reproduction blastula is implanted, and the kit comprises products of expression quantities of 398 genes as shown in a table 2 in wall trophoblast cells and polar trophoblast cells. The human assisted reproduction blastula is normally developed, and a pregnant woman normally delivers if the expression quantity of each gene in the 398 genes of the wall trophoblast cells of the human assisted reproduction blastula (a blastula implanted before fifth days of in-vitro fertilization) is twice or more or half or less that of the same gene in the 398 genes of the polar trophoblast cells. The kit has important application values for scientific judgment of transplantation strategies and decisions and possibility reduction of perinatal period complications of pregnant women and sick children in assisted reproduction.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a kit for screening high-quality blastocysts. Background technique [0002] Embryo implantation has become an unresolved hot issue in the field of reproductive medicine research. Embryo implantation is a key link in the reproductive process of mammals. It refers to the process in which blastocysts travel to the uterus and interact with the endometrium. It mainly includes the positioning, adhesion and penetration of blastocysts. This process is quite complicated. It is completed by the joint action of both the embryo and the endometrium. The trophoblast cells located on the periphery of the embryo play a key role in the process of embryo invasion. It performs the pregnancy recognition function by secreting a series of cytokines and hormones, and also secretes these factors and hormones. The role of the embryo to the endometrial adhesion and invasion. [0003] Studies on embryo implant...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/158
Inventor 赵亮孙丽芳
Owner BEIJING JISHUITAN HOSPITAL
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