Preparation method and application of blastocystis hominis stained specimen

A technology for Blastocystis hominis and dyed specimens, which is applied in the preparation of test samples, measuring devices, individual particle analysis, etc., can solve the problems of low identification of Blastocystis hominis, achieve high positive detection accuracy, and detect Accurate, low-handling effects

Pending Publication Date: 2022-01-14
中山大学附属第八医院
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Problems solved by technology

For this reason, the present invention proposes a preparation method and application of a dyed specimen of Blastocystis hominis, which can overcome the defect of low recognition of Blastocystis hominis in dyed samples in conventional detection methods, thereby improving the detection rate of Blastocystis hominis. Detection accuracy, so that rapid on-site detection of Blastocystis hominis can be realized

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  • Preparation method and application of blastocystis hominis stained specimen
  • Preparation method and application of blastocystis hominis stained specimen
  • Preparation method and application of blastocystis hominis stained specimen

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Embodiment Construction

[0039] In order to make the invention, the technical solution, and its technical effects are more clear, the present invention will be described in further detail below. It should be understood that the specific embodiments described in this specification are merely intended to illustrate the invention.

[0040] The experimental materials and reagents used are all consumables and reagents that are conventionally available from the commercial pathway without specifically described.

[0041] Human bud vessel high temperature fast dry sheet detection

[0042] Specific steps are as follows:

[0043] (1) Use the collector to collect detection samples (in the art, conventionally serviced as a detection sample for detecting human bud vessels, therefore, conventional use of the feces collector collects feces (about 3 g). Add 5 ~ 8 ml of physiological saline or The commercially available feces dilution was mixed to fully dissolve or dilute to obtain a dilution of the feces.

[0044] (2) Ta...

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Abstract

The invention discloses a preparation method and application of blastocystis hominis stained specimen, and the method comprises the following steps: pushing a detection sample on the surface of a detection substrate to form a sheet, drying at 70 +/-5 DEG C, and carrying out Wright staining on the dried detection sample. The method is simple to operate, low in operation requirement on technicians, short in detection time and low in detection cost, and compared with a traditional detection method or conventional Wright staining, the positive detection accuracy is higher, the detection is more accurate, the method has excellent applicability to the conditions that the sample content is complicated, the requirement for detection accuracy is high, the sample size is large, and on-site rapid detection is needed.

Description

Technical field [0001] The present invention relates to the field of medical examination, and in particular to the preparation method of human bud vessel staining specimen and its application. Background technique [0002] Human bud vessel is a single-cell native organism that parasitized in human and animal intestines, which is worldwide. The human bud vessel can invade the intestinal mucosal epithelium. The clinical manifestations are different, and those who have infected or immunity impaired human bud vessel infection can have abdominal pain, diarrhea, nausea, etc. It may endanger life. [0003] In the relevant technique, the detection method of human bud vesicle is mainly physiological saline smear, iodine - liquid staining, in vitro culture and PCR product sequencing. When the physiological saline smear method and iodine liquid staining were detected by human bud vessels, the insufficient morphological testing personnel were confused with other contents such as human bud ve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N1/44G01N15/10
CPCG01N1/30G01N1/44G01N15/10G01N2015/1062
Inventor 刘小晴邹小红王慧婷李振华
Owner 中山大学附属第八医院
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