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104 results about "Trophoblastic cell" patented technology
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Trophoblasts are specialized cells of the placenta that play an important role in embryo implantation and interaction with the decidualised maternal uterus. The core of placental villi contain mesenchymal cells and placental blood vessels that are directly connected to the fetal circulation via the umbilical cord.
Methods and compositions for identifying a fetal cell
InactiveUS20100304978A1High expressionMicrobiological testing/measurementLibrary screeningCandidate Gene Association StudyTrophoblast
The present invention provides methods and compositions for specifically identifying a fetal cell. An initial screening of approximately 400 candidate genes by digital PCR in different fetal and adult tissues identified a subset of 24 gene markers specific for fetal nucleated RBC and trophoblasts. The specific expression of those genes was further evaluated and verified in more defined tissues and isolated cells through quantitative RT-PCR using custom Taqman probes specific for each gene. A subset of fetal cell specific markers (FCM) was tested and validated by RNA fluorescent in situ hybridization (FISH) in blood samples from non-pregnant women, and pre-termination and post-termination pregnant women. Applications of these gene markers include, but are not limited to, distinguishing a fetal cell from a maternal cell for fetal cell identification and genetic diagnosis, identifying circulating fetal cell types in maternal blood, purifying or enriching one or more fetal cells, and enumerating one or more fetal cells during fetal cell enrichment.
Owner:VERINATA HEALTH INC
Method of isolating cells and uses thereof
InactiveUS20050123914A1Rapid and non-invasive diagnosticHigh yieldMicrobiological testing/measurementArtificial cell constructsTrophoblastNon invasive
The present invention relates to a non-invasive method of retrieving and identifying cells particularly fetal cells and trophoblastic cells. The invention includes methods for use of the cells for identifying chromosomal abnormalities and mutations particularly for prenatal diagnosis by performing genetic diagnosis for chromosomal and single gene disorders. The invention also includes methods of confirming cells of fetal origin.
Owner:MONASH UNIV +1
Human trophoblast stem cells and use thereof
Existence of human trophoblast stem (hTS) cells has been suspected but unproved. The isolation of hTS cells is reported in the early stage of chorionic villi by expressions of FGF4, FGFR-2, Oct4, Thy-1, and stage-specific embryonic antigens distributed in different compartments of the cell. hTS cells are able to derive into specific cell phenotypes of the three primitive embryonic layers, produce chimeric reactions in mice, and retain a normal karyotype and telomere length. In hTS cells, Oct4 and fgfr-2 expressions can be knockdown by bFGF. These facts suggest that differentiation of the hTS cells play an important role in implantation and placentation. hTS cells could be apply to human cell differentiation and for gene and cell-based therapies.
Owner:ACCELERATED BIOSCI
Method for detecting cancers
The invention provides for the production of several humanized murine antibodies specific for the antigen LK26, which is recognized by the murine antibody LK26. This antigen is expressed in all choriocarcinoma, teratocarcinoma and renal cancer cell lines whereas it is not expressed on cell lines of leukaemias, lymphomas, neuroectodermally-derived and epithelial tumour cell lines (excepting a small subset of epithelial cell lines). Furthermore, whereas renal cancer cell lines express the LK26 antigen, normal renal epithelial cells do not. Similarly, with the exception of the trophoblast, all normal adult and fetal tissues tested are negative for the LK26 phenotype. The invention also provides for numerous polynucleotide encoding humanized LK26 specific antibodies, expression vectors for producing humanized LK26 specific antibodies, and host cells for the recombinant production of the humanized antibodies. The invention also provides methods for detecting cancerous cells (in vitro and in vivo) using humanized LK26 specific antibodies. Additionally, the invention provides methods of treating cancer using LK26 specific antibodies.
Owner:MEMORIAL SLOAN KETTERING CANCER CENT
Human trophoblast stem cells and use thereof
Existence of human trophoblast stem (hTS) cells has been suspected but unproved. The isolation of hTS cells is reported in the early stage of chorionic villi by expressions of FGF4, fgfr-2, Oct4, Thy-1, and stage-specific embryonic antigens distributed in different compartments of the cell. hTS cells are able to derive into specific cell phenotypes of the three primitive embryonic layers, produce chimeric reactions in mice, and retain a normal karyotype and telomere length. In hTS cells, Oct4 and fgfr-2 expressions can be knockdown by bFGF. These facts suggest that differentiation of the hTS cells play an important role in implantation and placentation. hTS cells could be apply to human cell differentiation and for gene and cell-based therapies.
Owner:ACCELERATED BIOSCI
Method for treating cancers
The invention provides for the production of several humanized murine antibodies specific for the antigen LK26, which is recognized by the murine antibody LK26. This antigen is expressed on all choriocarcinoma, teratocarcinoma and renal cancer cell lines whereas it is not expressed on cell lines of leukaemias, lymphomas, neuroectodermally-derived and epithelial tumor cell lines (excepting a small subset of epithelial cell lines). Furthermore, whereas renal cancer cell lines express the LK26 antigen, normal renal epithelial cells do not. Similarly, with the exception of the trophoblast, all normal adult and fetal tissues tested are negative for the LK26 phenotype. The invention also provides for numerous polynucleotide encoding humanized LK26 specific antibodies, expression vectors for producing humanized LK26 specific antibodies, and host cells for the recombinant production of the humanized antibodies. The invention also provides methods for detecting cancerous cells (in vitro and in vivo) using humanized LK26 specific antibodies. Additionally, the invention provides methods of treating cancer using LK26 specific antibodies.
Owner:MEMORIAL SLOAN KETTERING CANCER CENT
Human corneal limbal stem cell tissue engineering product nourished by fibroblast and preparing process thereof
InactiveCN1635115APromote differentiationTight adhesionArtificially induced pluripotent cellsNon-embryonic pluripotent stem cellsDiseaseTrophoblast
A human corneal limbal stem cell-amnion tissue engineering complex using human corneal limbal fibroblasts as the trophoblast is characterized in that: it is close to the human corneal epithelium form in histology form, and is analogous to the human corneal epithelium in immunology phenotype, its ultrastructure shows that the connection between the cells grows mature and the connection between the epithelia and the amnion carrier is a close conglutination. The culture method includes the following steps: (1) preparing the amnion for culturing the human corneal limbal stem cell in vitro; (2) preparing the human corneal limbal fibroblast trophoblast; (3) preparing the human corneal epithelia suspension; (4) culturing the human corneal limbal stem cell-amnion complex. The human corneal limbal stem cell-amnion complex has analogous phenotype to the human corneal epithelium, and provides a safe and effective biology tissue engineering product to treat corneal limbal stem cell defective eye diseases.
Owner:天津医科大学眼科中心
Method for generating primate trophoblasts
The first method to cause a culture of human and other primate stem cells to directly and uniformly differentiate into a committed cell lineage is disclosed. Treatment of primate stem cells with a single protein trophoblast induction factor causes the cells to transform into human trophoblast cells, the precursor cells of the placenta. Several protein factors including bone morphogenic protein 4 (BMP4), BMP2, BMP7, and growth and differentiation factor 5 can serve as trophoblast-inducting factors.
Owner:WICELL RES INST
Method for culturing induced pluripotent stem cells by using human mesenchymal stem cells as trophoblast
InactiveCN102161980ANo pollution in the processReduce pollutionSkeletal/connective tissue cellsArtificially induced pluripotent cellsHeterologousForeskin
The invention provides a method for culturing human induced pluripotent stem cells (iPSCs) by using human bone marrow mesenchymal stem cells as a trophoblast. The method comprises the following steps of: obtaining the human iPSCs from child foreskin fibroblasts by using third to fifth generation human bone marrow mesenchymal stem cells obtained through subculture and culture after thawing from low-temperature freezing, performing amplification culture, and inoculating into trophoblast cells to obtain the human iPSCs. In the method, the human iPSCs are cultured by using the human bone marrow mesenchymal stem cells as the trophoblast cells instead of mouse embryonic fibroblasts in the conventional method, so that the pollution of heterologous cells in a culture system is reduced, that the culture system can make the human iPSCs amplified in vitro is proved, biological characteristics and multipotency of the human iPSCs are maintained for a long time, and the possibility of clinical application of the human iPSCs is provided.
Owner:ZHEJIANG UNIV
Human umbilical cord blood hematopoietic stem cell high-efficiency in vitro amplification technology
InactiveCN102465112AThe proportion of differentiation is smallEfficient differentiationBlood/immune system cellsMitomycin CTrophoblast
The invention discloses a human umbilical cord blood hematopoietic stem cell (HSC) high-efficiency in vitro amplification technology. According to the invention, umbilical cord mesenchymal stem cells (MSCs) and umbilical cord blood plasma are used in combination for carrying out HSC in vitro amplification. A process comprises steps that: umbilical cord MSCs are cultured as a trophoblast; a special culture solution containing umbilical cord blood plasma is adopted as an amplification medium of the HSCs; and umbilical cord blood karyotes are adopted as amplification initiator cells. According to the invention, exogenous cell factors are not required to be added, treatments such as mitomycin C or gamma-ray 21Gy irradiation are not required by the trophoblast, no 3-dimensional technology is required, and the HSC high-efficiency amplification can be carried out. The technology is advantaged in low cost, convenient amplification, and low product immunogenicity. The technology is suitable for large-scaled productions. The practical application of the technology plays an important role in clinical treatments and researches.
Owner:ANHUI HUIEN BIOTECH
Preparation method used for high efficiency amplification of natural killer cells
InactiveCN108103020AFix security issuesEasy to operateBlood/immune system cellsCell culture active agentsSerum igeCD16
The invention relates to a preparation method used for high efficiency amplification of natural killer (NK) cells. The preparation method comprises following steps: peripheral blood is collected, andsingle karyocytes are separated; the karyocytes are introduced into a culture bottle treated via coating with CD16 monoclonal antibody and HER2 monoclonal antibody for culturing; IL-2, IL-15, IL-21, and inactivated autologous serum are introduced into a culture medium at the same time; the cells are transformed into a culture bottle free of coating treatment, liquid supply is carried out every 2 to 3 days; and at last the obtained NK cells are collected. According to the preparation method, no heterogenous serum or trophoblastic cell is adopted, operation process is simple, high NK cell yieldis achieved, and the clinical application value is high.
Owner:上海莱馥生命科学技术有限公司
Separation and culturing method of human epidermis stem cell
A process for separating and culturing human epidermal stem cells includes such steps as providing ectocytic matrix covering Petri dish, separating epidermal cells, preparing trophoblastic cells and screening and culturing epidermal stem cells.
Owner:陕西艾尔肤组织工程有限公司
Protein chip for female infertility detection and kit thereof
The present invention discloses a dedicated multi-index protein chip for female infertility detection and a kit thereof. The protein chip consists of a substrate, protein detection indexes arranged on the substrate in an array mode and a contrast coating for the detection indexes. The protein detection indexes cover ovary antigen (OAg), endometrium antigen (EAg), sperm antigen (SAg), zona pellucida antigen (ZAg), human chorionic gonadotropin antigen (HCG), trophoblast antigen (TAg) and cardiolipin antigen (CAg). The present invention overcomes the defects of the existing autoimmunological detection products on market, such as single detection function, lagged detection indexes and no integration. Besides the indexes of the product (patent application No: 200510102466.3), the protein chip also has the ACA index. The density of all indexes can be quantitatively detected, and the cross reaction of all indexes is eliminated. The present invention not only has high specificity and high sensitivity, but also has high accuracy and precision.
Owner:上海裕隆生物科技有限公司
Non-invasive prenatal genetic diagnosis using transcervical cells
InactiveUS20060040305A1Microbiological testing/measurementDisease diagnosisPrenatal diagnosisCervical cell
A non-invasive, risk-free method of prenatal diagnosis is provided. According to the method of the present invention cell specimens are subjected to molecular and morphological methods which allow trophoblast identification. Trophoblasts identified according to the teachings of the present invention can be further examined to thereby prenatally diagnosing a fetus. Also provided is a method of in situ chromosomal, DNA and / or RNA analysis of a prestained specimen by incubating the prestained specimen in ammonium hydroxide. Also provided is a method of identifying embryonic cells according to a nucleus / cytoplasm ratio of at least 0.3 and the presence of at least variably condensed chromatin.
Owner:MONALIZA MEDICAL
Sterility, infertility six-index integral investigating reaction plate and protein chip kit
The invention relates to an integrated detection reaction orifice plate and protein chip reagent box for detecting six sterility indexes. And the reaction orifice plate comprises a substrate and reaction orifices on the substrate and the reaction orifices comprise 3-384 sample orifices, 1-300 negative contrast orifices, and 1-300 positive quality control orifices, where at the bottom of each reaction hole is solid carrier, coated with micro lattice of Sperm antigen (SAg), Zona pellucida antigen (ZPAg), Endometrium antigen (EMAg), Ovary antigen (OAg), Trophoblast antigen (TAg), and Human Chorionic Gonadotropin antigen (HCGAg) antigens or more. And the reaction orifice plate and reagent can simply and conveniently, rapidly and accurately implement simultaneously detection of six sterility indexes for many persons.
Owner:汪宁梅
Conditional Cas9 expression induced swine trophoblastic cell line and establishment method and application thereof
InactiveCN105624194ATo achieve the purpose of conditional knockoutEasy to operateEmbryonic cellsFermentationPregnancyEmbryo
The invention discloses a conditional Cas9 expression induced swine trophoblastic cell line and an establishment method and application thereof. The method includes: subjecting a lentiviral vector, for controlling Cas9 gene expression, of a Tet-on system to virus packaging, concentrating and purifying; incubating lentivirus with a typical swine trophoblastic cell line, changing fluid, adding puromycin for drug screening, diluting cells to single ones, and culturing to obtain the single-cell-source swine trophoblastic cell line with Cas9 expression under Tet-on regulation. The swine trophoblastic cell line is simple in operation and convenient to use and has a potential utilization value in researching of key functional genes of some trophoblast cells and screening of swine gene target spots. The cell line is expected to be a significant cell material for researching swine placenta development and applicable to correlation researches on swine early-stage placenta development and uterine pregnancy mechanisms and also has a high reference value in research of human early embryonic development.
Owner:AGRO BIOLOGICAL GENE RES CENT GUANGDONG ACADEMY OF AGRI SCI
Method for separating and purifying human embryo trophoblast and placental mesenchymal stem cells
InactiveCN105087470AAvoid damageLow costSkeletal/connective tissue cellsEmbryonic cellsSerum free mediaRed blood cell
The invention discloses a method for separating and purifying human embryo trophoblast and placental mesenchymal stem cells. The method includes: processing a placenta sample to obtain processed placenta tissues; digesting the tissues to obtain cell suspension; performing discontinuous Percoll density gradient separation to obtain trophoblast and placental mesenchymal stem cells; purifying the trophoblast; further separating and culturing the placental mesenchymal stem cells. The method has the advantages that HyQTase and DNAse I are used to jointly digest the tissues, and a large amount of trophoblast and placental mesenchymal stem cells with ideal cell purity and activity is obtained through the density gradient separation; digestion fragments, fibroblast and red blood cells are removed, the trophoblast and the placental mesenchymal stem cells are distinguished from each other, the purity of the trophoblast can reach 90%, the non-adherence upper layers of the placental mesenchymal stem cells are removed through a differential adhesion method, serum-free medium is added into a culture flask to continue the culture of the placental mesenchymal stem cells, and high purity of the placental mesenchymal stem cells is achieved.
Owner:大连金玛健康产业发展有限公司
Isolating fetal trophoblasts
InactiveUS20070224597A1Fast and accurate analysisEfficient captureCell dissociation methodsMicrobiological testing/measurementTrophoblastHydrolysis
Methods for isolating and purifying fetal trophoblasts from a mucus sample obtained from the uterine cavity of a pregnant female. The mucus sample is transported from a clinical collection facility to a laboratory in a transportation medium so the cells remain viable. The mucus sample is then subjected to precise processing steps, including treatment with mucolytic agents or mucinases, sugar hydrolysis enzymes, nucleases, and proteases to provide fetal cells, the outer surfaces of which are so essentially completely devoid of attached mucosal biological material that they are then isolated in greater numbers than previously had been possible. The isolated cells are in appropriate condition to immediately be effectively subjected to FISH or to other molecular diagnostics.
Owner:NOVARTIS AG
Separation and culturing method of human epidermis stem cell
Owner:陕西艾尔肤组织工程有限公司
Umbilical cord blood Treg cell in-vitro amplification method based on trophoblastic cells and application
ActiveCN112458053APromote amplificationRaise the ratioAntipyreticDigestive systemAutoimmune diseaseTrophoblast
The invention discloses an umbilical cord blood Treg cell in-vitro amplification method based on trophoblastic cells and application. The specific technical method comprises the steps that firstly, umbilical cord Wharton's jelly mesenchymal stem cells are adopted as the trophoblastic cells to induce preliminary proliferation of Treg cells in umbilical cord blood mononuclear cells; then, pure Tregcells are obtained through magnetic bead sorting; and finally, the Treg cells are stimulated to be rapidly amplified by using optimized amplification factors. According to the amplification method, human AB plasma, IL-2, rapamycin, an RARA agonist and a DNA methyltransferase inhibitor are used as the optimized amplification factors, and a large number of umbilical cord blood Treg cells with high purity and high activity can be prepared within two weeks. Umbilical cord blood is used as a raw material for Treg cell amplification, batch preparation can be achieved, and Treg cell quality fluctuation caused by individual differences of samples can be reduced. The umbilical cord blood Treg cells have low immunogenicity and can be used as universal cells for clinical research, such as autoimmunediseases, graft-versus-host diseases and the like.
Owner:成都云测医学生物技术有限公司
Noninvasive detection method for screening healthily grown blastulas
ActiveCN105861658AHigh precisionRelieve painMicrobiological testing/measurementEmbryo transferPloidy
Owner:GUANGZHOU NVWA LIFE TECH CO LTD
Differential staining method for inner cell mass cells and trophoblastic cells of cattle blastulae
InactiveCN102426126APromote research progressPreparing sample for investigationFluorescent stainingMonoclonal antibody
The invention discloses a differential staining method for inner cell mass cells and trophoblastic cells of cattle blastulae. The method comprises the following steps: 1, carrying out immune combination on an anti-CDX2 monoclonal antibody which is adopted as a primary antibody and CDX2 molecules which are specifically expressed in the trophoblastic cells of the cattle blastulae through an immunostaining process; 2, the cattle blastulae which are cleaned and are combined with the primary antibody are immunostained with an antibody which is labeled by red fluorescence and can combine with the anti-CDX2 monoclonal antibody as a secondary antibody through the immunostaining process; and 3, whole nuclei of the cattle blastulae are subjected to fluorescent staining after completing the immunostaining to form the differential staining. On the basis of a case that CDX2 proteins specifically express in the trophoblastic cells and do not express in the inner cell mass cells, the method of the invention allows the differential staining to be carried out based on the red immunostaining of the CDX2 molecules and the blue staining of DAPI nuclei, so the accuracy is 100%, and obtained pictures are intelligible and beautiful.
Owner:NORTHWEST A & F UNIV
Serum-based, diagnostic, biological assay to predict pregnancy disorders
ActiveUS20110059904A1Simple, non-invasiveCost effectiveMicrobiological testing/measurementDisease diagnosisTrophoblastBiology
The invention provides serum-based, diagnostic, biological assays for predicting disorders of pregnancy resulting from poor trophoblast and / or placental ischemia, including preeclampsia. Serum samples from such subjects exhibit an ability to disrupt the architecture involving fetal trophoblasts and maternal endothelial cells in a three-dimensional, dual cell co-culture system provided herein, in contrast to normal pregnancy serum samples. Based on these distinctions, the assays are employed to predict pregnancy outcomes as early as first trimester.
Owner:WOMEN & INFANTS HOSPITAL OF RHODE ISLAND
Use of Activin A in human embryo stem cell trophoblast-free cell culture
The invention discloses the application of Activin A for cell culture in human blast stem cell panoistic layer, belonging to biomedical technology field. It is necessary and adequate for Activin A to maintain self- refresh and multi-function for human blast stem cell, it can induce expression of transcription factor Oct4 and Nanog. The stem cell still possess differentiation potence in mouse body for teratoma after 10- generation culture with panoistic cell culture medium containing 5 ng / ml Activin A, and it still maintains normal karyotype after 150- day culture and more than 20- generation.
Owner:浙江煦顼技术有限公司
Method for separating placental trophoblastic cells by using immunomagnetic bead process
InactiveCN107312747AAvoid the inconvenience of expanding cultivationShort-term rapid enrichmentCell dissociation methodsEmbryonic cellsMagnetic beadTrophoblastic cell
The invention discloses a method for separating placental trophoblastic cells by using an immunomagnetic bead process. The method comprises the following steps: (1) preparing an immunomagnetic bead-coupled specific antibody by adding a coupling agent and an antibody capable of specifically capturing placental trophoblastic cells into immunomagnetic beads and carrying out incubation; (2) washing the antibody-coupled immunomagnetic beads obtained in the step (1) twice, and then preserving the antibody-coupled immunomagnetic beads in a preservation solution for subsequent use; (3) collecting a placental trophoblast sample and preparing a sample cell suspension by using an enzymolysis process; (4) washing the sample cell suspension obtained in the step (3) twice; (5) adding the antibody-coupled immunomagnetic beads treated in the step (2) and carrying out incubation; and (6) washing the immunomagnetic beads having undergone incubation in the step (5) twice and discarding a supernatant so as to obtain purified placental trophoblast cells. According to the invention, immunomagnetic bead cell sorting technology is employed, and the antibody-coupled immunomagnetic beads are used for specific capture of placental trophoblast cells, so trophoblastic cells can be easily and rapidly separated via a few simple steps, and automation can be easily realized.
Owner:PILOT GENE TECH HANGZHOU CO LTD
Method for separating trophoblastic cells
PendingCN111304153AEarly collection timeReduce infectionMicrobiological testing/measurementEmbryonic cellsImmunofluorescenceTrophoblastic cell
The invention provides a method for separating trophoblastic cells. The method comprises the following steps: obtaining specific antigens expressed on surfaces of various types of trophoblastic cellsaccording to searched documents, and screening the obtained specific antigens; confirming expression of the antigens in the trophoblastic cells through immunohistochemistry, and determining an antibody combination system through immunofluorescence; adding a couplant and an antibody combination into immunizing magnetic beads, and carrying out coupling; collecting a trophoblast sample from a placenta, preparing a sample cell suspension from the sample, and adding the immunizing magnetic beads with a specific antibody for incubation; and washing the immunizing magnetic beads after the incubationstep is completed, thereby obtaining separated purified trophoblastic cells of the placenta. Compared with traditional amniocentesis and chorionic villus sampling, the method has a noninvasive advantage, the material drawing time is relatively early, the risk of infection and abortion is low, and detection results have similar reliability.
Owner:GUANGDONG HYBRIBIO BIOTECH CO LTD +2
Primate embryonic stem cells
InactiveUS20050158854A1High nucleus/cytoplasm ratioNervous disorderMetabolism disorderGerm layerSurface marker
A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (−); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a particularly advantageous embodiment, the cells of the preparation are human embryonic stem cells, have normal karyotypes, and continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). A method for isolating a primate embryonic stem cell line is also disclosed.
Owner:THOMSON
B lymphocyte in vitro culture system and applications thereof
ActiveCN111518765AImprove biological activityUniversalGenetically modified cellsImmunoglobulinsT lymphocyteTrophoblast
The invention belongs to the technical field of primary cell culture, and especially relates to a B lymphocyte in vitro culture system and applications thereof. The culture system utilizes a T lymphocyte line capable of secreting interleukin molecules such as IL-2 as trophoblastic cells so as to provide micro interleukin molecules required by the growing development of B lymphocyte; and trimer CD40L molecules expressed in vitro through a recombinant manner and having high biological activity are added as important molecules so as to further stimulate the growth, reproduction and development ofthe B lymphocyte. The system can also perform gamma ray irradiation treatment on the trophoblastic cells, so that the pre-treated trophoblastic cells can lose the ability of cell reproduction but still has the ability of protein synthesis and secretion within 7-14 days. The provided system can effectively culture the B lymphocyte derived from rabbits and alpacas in vitro, and can utilize the method to develop corresponding monoclonal antibodies.
Owner:优睿赛思(武汉)生物科技有限公司
Method for efficiently separating mouse spermatogonial stem cells
InactiveCN105969723AHigh separation purityIncrease the number ofGerm cellsEnzyme digestionMale infertility
The invention discloses a method for efficiently separating mouse spermatogonial stem cells. The method includes the following steps that firstly, mouse testis are collected, and a testicular cell suspension is prepared through a two-step enzyme digestion method; secondly, the testicular cell suspension is inoculated in a porous plate according to a certain density, and a spermatogonial stem cell culture solution is added for in-vitro culture; thirdly, the culture solution is removed, testis cells of different times are cultured through tryptic digestion, and spermatogonial stem cells are sorted from the testis cells through the magnetic activated cell sorting; fourthly, the sorted spermatogonial stem cells are inoculated to an STO trophoblast, and a spermatogonial stem cell culture solution is added for primary culture and subculture of the spermatogonial stem cells. A mouse spermatogonial stem cell system is built through the method including the steps of separation, culture, re-separation and re-culture. The method can achieve the aim of efficiently enriching the spermatogonial stem cells in vitro, and is economical and easy to implement, so that a direction is provided for protection of rare animal varieties and treatment of male infertility.
Owner:INNER MONGOLIA UNIVERSITY
Human placental nutritive layer cell line and its use
InactiveCN1935990AProliferativeActive growthVertebrate cellsMammal material medical ingredientsDiseaseEmbryo
The invention relates to human placenta trophoblastic cell series which is conserved in China Committee for Culture Collection of Microorganisms on September 22th in 2005 with CGMCC No.1461 conserving number. It includes the following steps: separating the trophoblastic cell form the placenta tissue which is pregnant for 6-7 weeks; filtering the cells with multiplication capacity; processing subunit gene transfection by telomere enzyme to gain four long life cell strains. The cell series has normal multiplication capacity and biological chemical function, active cell growth, and reaches the 210 generation. Thus it can be used as target cell model for filtering antifertility drug and curing pregnant correlative disease medicine, provide ideal vitro model for further studying trophoblastic cell, lay important foundation for studying embryo implantation and pregnant physiology and pathological mechanisms.
Owner:INST OF ZOOLOGY CHINESE ACAD OF SCI
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