4 results about "Trophoblastic cell" patented technology

The invention discloses a trophoblast-free one-step culture method and application of tumor infiltrating lymphocytes, and particularly discloses a one-step amplification method of tumor infiltrating lymphocytes, which comprises the following steps: treating a tumor sample obtained from a subject into tumor segments; the tumor segments are cultured in a culture medium containing a CD3 agonist, a 4-1BB agonist and IL-2, so that an amplified tumor infiltration lymphocyte population is obtained, and the culture medium does not contain trophoblastic cells. Compared with the prior art, the method has the advantages that the required period for amplifying the tumor infiltrating lymphocytes is shorter, the cost is lower, the cell effect function is stronger, and the clinical application range of the tumor infiltrating lymphocyte therapy is widened.

The invention provides an application of DCTPP1 in detection of an auditing abortion kit, relates to the technical field of target spot diagnosis, and particularly provides an application of DCTPP1 as a target spot in preparation of an auditing abortion detection product, and an experimental result shows that DCTPP1 is one of key genes for auditing abortion. Specifically, after the DCTPP1 is knocked down from a human embryo trophoblast cell line HTR8 / SVneo, compared with a control group, the reduction of the DCTPP1 can cause more cell oxidation stress increase, and cell viability is reduced 24 hours later, and finally cell apoptosis occurs; in the mechanism, the DCTPP1 interacts with the RNA binding protein AUF1, and the reduction of the DCTPP1 causes the increase of oxidative stress of human embryo trophoblastic cells through the AUF1, so that apoptosis is caused. Experimental results in the application show that DCTPP1 is one of key genes for auditing abortion, and cell apoptosis can be finally caused by destroying the function of DCTPP1 in embryonic trophoblast cells through technologies such as transfection of specific siRNA and the like. In addition, the DCTPP1 knocked-down embryo trophoblast cells can be subjected to apoptosis, and the DCTPP1 knocked-down embryo trophoblast cells are one of useful genes for detecting and auditing abortion.

The invention discloses an establishment method and application of a placenta trophoblast organoid model with correct physiological polarity, belongs to the technical field of organoid and stem cell model establishment, and aims to solve the problem of structural polarity error in trophoblast cells at present. The establishment method comprises the following specific steps: step 1, inducing nave embryonic stem cells to differentiate hTSC hollow spheres; step 2, transferring the hTSC hollow sphere into matrigel for culture to obtain a placenta trophoblast organ; and 3, transferring the placenta trophoblast-like organs into the 2D maintenance system to maintain the growth of the placenta trophoblast-like organs. According to the invention, the internal cavity of the placenta villus trophoblast and the physiological polar structure in which STB is outside and CTB is inside in trophoblast cells can be simulated, and the placenta trophoblast organ with three cell types of CTB, STB and EVT can be constructed at the same time.

Provided is a mammalian derived cell model which can be used in anti-aging drugs. The mammalian-derived extracembryonic trophoblast cell model is an early mammalian-derived extracembryonic stage. The change model of differentiation of cells into exoembryonic trophoblastic cells / organoids and the aging evaluation index system can be used various anti-aging research and target testing, anti-aging drug detection, screening, identification and innovative drugs / natural products Research and development of chemicals, and / or health foods and additives. It is helpful to screen and identify many resistances Candidate targets for aging can be further mechanized and functionally validated for future therapeutic use. The Screening of anti-aging drugs or natural products will produce huge economic and social benefits.