34results about How to "Efficient differentiation" patented technology

A system detects at least one of nuclear and fissile materials. The system includes a plurality of high speed scintillator detectors. Each high speed scintillator detector in the plurality of high speed scintillator detectors includes at least one photo sensor and a pre-amp circuit adapted to eliminate pulse stretching and distortion of detected light pulses emitted from scintillation material when interacting with neutron particles and/or gamma particles. An isotope database includes a plurality of spectral images corresponding to different known isotopes. An information processing system is adapted to compare spectral data received from each high speed scintillator detector to one or more of the spectral images and identify one or more isotopes present in an object or container being monitored.

The invention relates to the field of stem cell biology, specifically to a method for differentiating human pluripotent stem cells into natural killer cells and an application. The invention disclosesa pluripotent stem cell-derived natural killer cell. The pluripotent stem cell-derived natural killer cell expresses CD56, Nkp30, Nkp44 and Nkp46, and also expresses markers CD16 and CD94 of a maturenatural killer cell. The invention also discloses a method for preparing the natural killer cell. The method comprises the following steps: S1, formation of an embryoid body; S2, differentiation of the embryoid body into hematopoietic progenitor cells; S3, differentiation of the hematopoietic progenitor cells into NK cells; and S4, maturation and expansion of the NK cells. With the differentiation method provided by the invention, the pluripotent stem cells can be rapidly, efficiently, simply and conveniently induced to be differentiated into the natural killer cells with low cost on the basis of a culture medium with definite components and an optimized cell factor combination.

The present invention provides compositions comprising mesenchymal stem cells (MSCs), and methods for their novel use in the repair of cardiac damage and treatment of inflammatory diseases. The invention also provides methods for using TSG-6 protein that is secreted by MSCs under certain conditions, for repair of cardiac damage and inflammatory disease. The compositions of the invention may be particularly useful in restoring cardiac function following cardiac damage, including, but not limited to, myocardial infarction, as well as in reducing symptoms of inflammatory disease.

The invention discloses a human umbilical cord blood hematopoietic stem cell (HSC) high-efficiency in vitro amplification technology. According to the invention, umbilical cord mesenchymal stem cells (MSCs) and umbilical cord blood plasma are used in combination for carrying out HSC in vitro amplification. A process comprises steps that: umbilical cord MSCs are cultured as a trophoblast; a special culture solution containing umbilical cord blood plasma is adopted as an amplification medium of the HSCs; and umbilical cord blood karyotes are adopted as amplification initiator cells. According to the invention, exogenous cell factors are not required to be added, treatments such as mitomycin C or gamma-ray 21Gy irradiation are not required by the trophoblast, no 3-dimensional technology is required, and the HSC high-efficiency amplification can be carried out. The technology is advantaged in low cost, convenient amplification, and low product immunogenicity. The technology is suitable for large-scaled productions. The practical application of the technology plays an important role in clinical treatments and researches.

A purpose of the present invention is to provide a small chemical compound which especially promotes induction of the differentiation of ES cells into insulin-producing cells. Another purpose of the present invention is to provide: a method for inducing the differentiation of ES cells into insulin-producing cells using the compound; and an agent for promoting induction of the differentiation into insulin-producing cells. Another purpose of the present invention is to provide the thus-induced insulin-producing cells. Provided are: an agent for promoting induction of the differentiation of stem cells derived from a mammal into insulin-producing cells, which contains a compound that is selected from the group consisting of dopamine metabolism inhibitors, serotonin metabolism inhibitors, acetylchotine, acetylcholine degrading enzyme inhibitors and acetylcholine receptor activators; and a method for inducing differentiation using the agent for promoting induction of differentiation.

Disclosed herein is a cell culture apparatus that can achieve appropriate culture conditions. The cell culture apparatus (1) includes: a cylindrical culture vessel (10) that holds a culture liquid containing cells; a supporting column (20) that stands upright in a center of an inner surface of a bottom (12) in the culture vessel; and a stirring device (30) that includes an attaching portion (32) that is attached to an upper portion of the supporting column so as to be rotatable relative to the supporting column and a stirring blade (34) whose upper portion is fixed to the attaching portion so as to rotate around the supporting column.

The invention provides a screening method for IncRNAs which are in differential expression during chondrogenic differentiation of ADSCs, ADSCs with high cartilage differentiation capacity, and an induced differentiation method for cartilage cells. The screening method for IncRNAs comprises the following steps: gradient induced differentiation of ADSCs chondroblasts, IncRNAs sequencing of ADSCs, and analysis of IncRNAs sequencing results. The IncRNAs included in the ADSCs comprise at least one selected from the group consisting of H19, Scube3 and NONRATG00281. The ADSCs with high cartilage differentiation capacity are modified by the IncRNAs in differential expression.

A high accuracy information extracting device construction system includes: a feature quantity extraction expression list generating unit for generating a feature quantity extraction expression list; a feature quantity calculating unit for calculating feature quantities of teacher data by means of respective feature quantity extracting expressions; a teacher data supply unit for supplying teacher data; an evaluation value calculating unit for generating information extracting expressions by means of machine learning on the basis of the calculated feature quantities of teacher data and the teacher data, and calculating evaluation values for the respective feature quantity extracting expressions; and a synthesis unit for constructing a high accuracy information extracting device using T weak information extracting parts F(X)t output from the evaluation value calculating unit 15 and confidence levels Ct corresponding thereto.

The invention belongs to the technical field of medical instruments, and particularly relates to a double-sided heterogeneous degradable metal film and a preparation method thereof. The double-sided heterogeneous degradable metal film comprises a degradable metal matrix, a first film layer and a second film layer, the first film layer and the second film layer are arranged on the opposite surfaces of the metal matrix, the first film layer is a calcium phosphate coating and is arranged on the first surface of the metal matrix, and the second film layer is a calcium phosphate coating and is arranged on the second surface of the metal matrix, the second film layer is a polymeric film layer and is arranged on the second surface of the metal matrix, and the polymeric film layer has the functions of selectively promoting adhesion, migration and differentiation of bone cells and bone regeneration. The double-sided heterogeneous degradable metal film can simultaneously meet the two functional requirements of resisting bacteria and promoting bone cell regeneration, and the functional requirements of anisotropy of the two sides of the barrier film are met.

The invention belongs to the technical field of chemical synthesis, and particularly relates to a preparation method of an anti-tumor indole compound. The preparation method comprises the following steps: obtaining a compound shown in a formula II and a compound shown in a formula III, dissolving the compound shown in the formula II and the compound shown in the formula III in an organic solvent,and carrying out a synthetic reaction under a condition that the temperature is 0-40 DEG C and a catalyst is chiral phosphoric acid to obtain the anti-tumor indole compound shown in a formula I, wherein R<1> and R<2> are ones separately selected from alkyl, aryl, heteroaryl, substituted aryl and substituted heteroaryl; Ar<1>, Ar<2> and Ar<3> are ones separately selected from aryl, heteroaryl and substituted heteroaryl; and the Ar<1>, the Ar<2> and the Ar<3>differ from each other. The preparation method provided by the invention has the characteristics of simple and practical operation, high yield, greenness and economy, environmental friendliness and easy industrialization. The formula II the formula III and the formula I are represented as in the description.

The invention relates to the technical field of mesenchymal stem cells, in particular to an induction system capable of efficiently promoting chondrogenic differentiation of mesenchymal stem cells. The induction system comprises a first induction culture medium and a second induction culture medium, the first induction culture medium comprises a growth culture medium and KGN, the concentration ofthe KGN in the first induction culture medium is 0.5-3 micromole/L, the second induction culture medium comprises a cartilage-induced differentiation culture medium and an additive, the additive comprises the KGN, and the concentration of the KGN in the second induction culture medium is 10-500 nmol/L. The mesenchymal stem cells are firstly pretreated through the KGN, and then induction is conducted on the cells through a low concentration KGN induction system, and mesenchymal stem cells can perform efficient differentiation on cartilage cells, good cartilage cells can be formed only within eight days, the culture time is short, the cost is low, and the system takes effect significantly.

The invention provides pluripotent human stem cell technology, method and product of small molecule oriented tissue and organ regeneration. The technology provided in the invention can directly and high-effectively convert pluripotent human stem cells into high-purity human cells in specific pedigree in large scale, which are high in quality, are clinical-grade and have no foreign matters, through small molecule oriented differentiation induction. The invention provides an approach of producing or manufacturing the pluripotent human stem cells, which are high in quality, are clinical-grade and have no foreign matters, and a functional human cell therapy derivative thereof in large scale for application of cell therapy and commercialization. In particular, the invention creatively provides a new commercial source of high-purity functional human neurons and myocardial cells for medical treatment of repairing central nervous system or cardiac muscle.

The invention relates to a 3D pseudo-skin construction method based on organs-on-chips and directional differentiation of induced pluripotent stem cells. According to the method, a 3D spinning supportis placed in sterilized organs-on-chips, fibroblast is inoculated on one surface of the 3D spinning support, keratinocyte is inoculated on the other surface of the 3D spinning support, then two culture mediums (CnT-07 epidermal keratinocyte medium and DMEM with 10 vol.% of FBS and 1 vol.% of penicillin-streptomycin) are introduced into the organs-on-chips to culture fibroblast and keratinocyte respectively, and the co-culture of fibroblast and keratinocyte and cell proliferation are realized. The construction method utilizes a stem cell differentiation technology to obtain required cells, thecell inoculation is performed on a nano structure, fibroblast and keratinocyte are co-cultured in organs-on-chips, and the method is used for epidermal tissue repair, is used to establish a drug model, and is the hot spot of research at present.

The present invention belongs to the field of stem cell biology, relates to lineage-specific differentiation of human multipotential or pluripotent stem cells, and particularly relates to a mesenchymal stem cell, and a preparation method and an application thereof. The method for preparing the mesenchymal stem cell comprises the following steps: S1, human multipotential stem cells form embryoid bodies; S2, the embryoid bodies differentiate into mesoderm cells; and S3, the mesodermal cells differentiate into the mesenchymal stem cells. The preparation method has a clear cell differentiation path, high differentiation efficiency, and stable differentiation effect and does not use serum-containing culture system or trophoblast cells, and the obtained cell population has high purity and largenumber, and thus is suitable for subsequent production and application of clinical-grade cell preparations.

The invention relates to the technical field of plant tissue culture, in particular to a method for regenerating prunus mume plants in a somatic embryo way and a special culture medium of the method. The invention provides the method for regenerating the prunus mume plants in the somatic embryo way. The method comprises the following steps: culturing prunus mume explants on a somatic embryo induction culture medium to obtain somatic embryos, and proliferating the somatic embryos on a somatic embryo proliferation culture medium to obtain secondary somatic embryos, and culturing the secondary somatic embryos in a somatic embryo germination culture medium to form plants. According to the method, the efficiency of generating the somatic embryos through induction of explants is high, the somatic embryos can be continuously proliferated in a secondary somatic embryo form, the proliferation effect is good, long-term subculture preservation can be achieved, the ability of the somatic embryos to be differentiated into plants can be kept, and the somatic embryos can be efficiently differentiated and regenerated into prunus mume plants after being cultured through the germination culture medium.

The invention discloses a method for inducing and amplifying decidua-like natural killer (NK) cells from hematopoietic stem cells in vitro. The method comprises the following steps: culturing CD34+ hematopoietic stem cells in four stages in vitro, namely amplifying the CD34+ hematopoietic stem cells in stage I, inducing differentiation of the CD34+ hematopoietic stem cells into NK cell precursors in stage II, inducing differentiation of the NK cell precursors into NK cells in stage III, inducing amplification and maturation of the NK cells and collecting CD3-CD56+NK cells in stage IV, and thus obtaining decidua-like natural killer cells, wherein the culture is started from a fresh basic culture medium containing a cytokine combination I, fresh basic culture mediums containing the cytokine combination I, combination II, combination III and combination IV are used in culture stages I, II, III and IV respectively for half-quantity liquid change culture, the cytokine combination I is composed of Flt3L and SCF; the combination II is composed of Flt3L, SCF and IL-15; the combination III is composed of Flt3L, SCF and IL-15; and the combination IV is IL-15.

The invention relates to a method for inducing pluripotent stem cells to be differentiated into melanocytes in a serum-free culture solution, which comprises the following steps: preparing a serum-free matrix culture solution of differentiated cells, including a No.1 culture medium, a No.2 culture medium and a DiffMel culture medium; culturing the pluripotent stem cells in the cell culture plate until the pluripotent stem cells overgrow 80% of the cell culture plate; inducing the pluripotent stem cells to be differentiated into precursor cells of melanocytes in a matrix culture solution; a culture dish for treatment and passage is coated, and precursor cells of the melanocytes are continuously induced to be differentiated into the melanocytes in a DiffMel culture medium; and culturing the differentiated melanocytes. The differentiated melanocytes have cell characteristics similar to those of human primary melanocytes, and have great difference from malignant melanoma cells. Compared with the prior art, the melanocyte differentiation method provided by the invention is short in time consumption and high in differentiation efficiency, and serum or other animal-derived preparations are not needed, so that a basis is provided for future clinical application.

The invention belongs to the technical field of cell culture, and particularly relates to application of SAFit2 and a culture medium in promoting differentiation of human induced pluripotent stem cells to dopaminergic neurons. The invention provides an application of SAFit2 in promoting efficient differentiation of human induced pluripotent stem cells to dopaminergic neurons. The SAFit2 provided by the invention can promote efficient differentiation of human induced pluripotent stem cells into dopaminergic neurons.