34results about How to "Efficient differentiation" patented technology

A system detects at least one of nuclear and fissile materials. The system includes a plurality of high speed scintillator detectors. Each high speed scintillator detector in the plurality of high speed scintillator detectors includes at least one photo sensor and a pre-amp circuit adapted to eliminate pulse stretching and distortion of detected light pulses emitted from scintillation material when interacting with neutron particles and / or gamma particles. An isotope database includes a plurality of spectral images corresponding to different known isotopes. An information processing system is adapted to compare spectral data received from each high speed scintillator detector to one or more of the spectral images and identify one or more isotopes present in an object or container being monitored.

The invention relates to the field of stem cell biology, specifically to a method for differentiating human pluripotent stem cells into natural killer cells and an application. The invention disclosesa pluripotent stem cell-derived natural killer cell. The pluripotent stem cell-derived natural killer cell expresses CD56, Nkp30, Nkp44 and Nkp46, and also expresses markers CD16 and CD94 of a maturenatural killer cell. The invention also discloses a method for preparing the natural killer cell. The method comprises the following steps: S1, formation of an embryoid body; S2, differentiation of the embryoid body into hematopoietic progenitor cells; S3, differentiation of the hematopoietic progenitor cells into NK cells; and S4, maturation and expansion of the NK cells. With the differentiation method provided by the invention, the pluripotent stem cells can be rapidly, efficiently, simply and conveniently induced to be differentiated into the natural killer cells with low cost on the basis of a culture medium with definite components and an optimized cell factor combination.

The present invention provides compositions comprising mesenchymal stem cells (MSCs), and methods for their novel use in the repair of cardiac damage and treatment of inflammatory diseases. The invention also provides methods for using TSG-6 protein that is secreted by MSCs under certain conditions, for repair of cardiac damage and inflammatory disease. The compositions of the invention may be particularly useful in restoring cardiac function following cardiac damage, including, but not limited to, myocardial infarction, as well as in reducing symptoms of inflammatory disease.

The invention discloses a human umbilical cord blood hematopoietic stem cell (HSC) high-efficiency in vitro amplification technology. According to the invention, umbilical cord mesenchymal stem cells (MSCs) and umbilical cord blood plasma are used in combination for carrying out HSC in vitro amplification. A process comprises steps that: umbilical cord MSCs are cultured as a trophoblast; a special culture solution containing umbilical cord blood plasma is adopted as an amplification medium of the HSCs; and umbilical cord blood karyotes are adopted as amplification initiator cells. According to the invention, exogenous cell factors are not required to be added, treatments such as mitomycin C or gamma-ray 21Gy irradiation are not required by the trophoblast, no 3-dimensional technology is required, and the HSC high-efficiency amplification can be carried out. The technology is advantaged in low cost, convenient amplification, and low product immunogenicity. The technology is suitable for large-scaled productions. The practical application of the technology plays an important role in clinical treatments and researches.

A purpose of the present invention is to provide a small chemical compound which especially promotes induction of the differentiation of ES cells into insulin-producing cells. Another purpose of the present invention is to provide: a method for inducing the differentiation of ES cells into insulin-producing cells using the compound; and an agent for promoting induction of the differentiation into insulin-producing cells. Another purpose of the present invention is to provide the thus-induced insulin-producing cells. Provided are: an agent for promoting induction of the differentiation of stem cells derived from a mammal into insulin-producing cells, which contains a compound that is selected from the group consisting of dopamine metabolism inhibitors, serotonin metabolism inhibitors, acetylchotine, acetylcholine degrading enzyme inhibitors and acetylcholine receptor activators; and a method for inducing differentiation using the agent for promoting induction of differentiation.

The invention belongs to the field of biological medicines, and discloses a composition for promoting hair cell regeneration and hearing recovery and an application thereof. The composition comprisesa Wnt agonist and one or more of the following reagents: (a) a VEGFR inhibitor; (b) a Tgfbr inhibitor; and (c) an ERG inhibitor. The VEGFR inhibitor comprises regorafenib, apatinib mesylate, cabozantinib, pazopanib hydrochloride and medicinal salts or derivatives of the regorafenib, the apatinib mesylate, the cabozantinib and the pazopanib hydrochloride. An organoid platform verifies that after any inhibitor in a signal axis of EGFR-TGFB1-ERG is combined with the Wnt agonist to prepare the composition, efficient hair cell differentiation, maturation and survival can be realized, and the composition has important value for hearing recovery.

Disclosed herein is a cell culture apparatus that can achieve appropriate culture conditions. The cell culture apparatus (1) includes: a cylindrical culture vessel (10) that holds a culture liquid containing cells; a supporting column (20) that stands upright in a center of an inner surface of a bottom (12) in the culture vessel; and a stirring device (30) that includes an attaching portion (32) that is attached to an upper portion of the supporting column so as to be rotatable relative to the supporting column and a stirring blade (34) whose upper portion is fixed to the attaching portion so as to rotate around the supporting column.

The invention provides a screening method for IncRNAs which are in differential expression during chondrogenic differentiation of ADSCs, ADSCs with high cartilage differentiation capacity, and an induced differentiation method for cartilage cells. The screening method for IncRNAs comprises the following steps: gradient induced differentiation of ADSCs chondroblasts, IncRNAs sequencing of ADSCs, and analysis of IncRNAs sequencing results. The IncRNAs included in the ADSCs comprise at least one selected from the group consisting of H19, Scube3 and NONRATG00281. The ADSCs with high cartilage differentiation capacity are modified by the IncRNAs in differential expression.

A high accuracy information extracting device construction system includes: a feature quantity extraction expression list generating unit for generating a feature quantity extraction expression list; a feature quantity calculating unit for calculating feature quantities of teacher data by means of respective feature quantity extracting expressions; a teacher data supply unit for supplying teacher data; an evaluation value calculating unit for generating information extracting expressions by means of machine learning on the basis of the calculated feature quantities of teacher data and the teacher data, and calculating evaluation values for the respective feature quantity extracting expressions; and a synthesis unit for constructing a high accuracy information extracting device using T weak information extracting parts F(X)t output from the evaluation value calculating unit 15 and confidence levels Ct corresponding thereto.

The invention belongs to the technical field of medical instruments, and particularly relates to a double-sided heterogeneous degradable metal film and a preparation method thereof. The double-sided heterogeneous degradable metal film comprises a degradable metal matrix, a first film layer and a second film layer, the first film layer and the second film layer are arranged on the opposite surfaces of the metal matrix, the first film layer is a calcium phosphate coating and is arranged on the first surface of the metal matrix, and the second film layer is a calcium phosphate coating and is arranged on the second surface of the metal matrix, the second film layer is a polymeric film layer and is arranged on the second surface of the metal matrix, and the polymeric film layer has the functions of selectively promoting adhesion, migration and differentiation of bone cells and bone regeneration. The double-sided heterogeneous degradable metal film can simultaneously meet the two functional requirements of resisting bacteria and promoting bone cell regeneration, and the functional requirements of anisotropy of the two sides of the barrier film are met.

The invention belongs to the technical field of chemical synthesis, and particularly relates to a preparation method of an anti-tumor indole compound. The preparation method comprises the following steps: obtaining a compound shown in a formula II and a compound shown in a formula III, dissolving the compound shown in the formula II and the compound shown in the formula III in an organic solvent,and carrying out a synthetic reaction under a condition that the temperature is 0-40 DEG C and a catalyst is chiral phosphoric acid to obtain the anti-tumor indole compound shown in a formula I, wherein R<1> and R<2> are ones separately selected from alkyl, aryl, heteroaryl, substituted aryl and substituted heteroaryl; Ar<1>, Ar<2> and Ar<3> are ones separately selected from aryl, heteroaryl and substituted heteroaryl; and the Ar<1>, the Ar<2> and the Ar<3>differ from each other. The preparation method provided by the invention has the characteristics of simple and practical operation, high yield, greenness and economy, environmental friendliness and easy industrialization. The formula II the formula III and the formula I are represented as in the description.

The invention relates to the technical field of mesenchymal stem cells, in particular to an induction system capable of efficiently promoting chondrogenic differentiation of mesenchymal stem cells. The induction system comprises a first induction culture medium and a second induction culture medium, the first induction culture medium comprises a growth culture medium and KGN, the concentration ofthe KGN in the first induction culture medium is 0.5-3 micromole / L, the second induction culture medium comprises a cartilage-induced differentiation culture medium and an additive, the additive comprises the KGN, and the concentration of the KGN in the second induction culture medium is 10-500 nmol / L. The mesenchymal stem cells are firstly pretreated through the KGN, and then induction is conducted on the cells through a low concentration KGN induction system, and mesenchymal stem cells can perform efficient differentiation on cartilage cells, good cartilage cells can be formed only within eight days, the culture time is short, the cost is low, and the system takes effect significantly.

The invention relates to a preparing method of a biological engineering film material, particularly implantable poly(1,8-octanediol-citrate) film modified through click chemistry and a preparing method thereof. The poly(1,8-octanediol-citrate) film modified through click chemistry is prepared by subjecting azide-group-containing poly(1,8-octanediol-citrate) and alkynyl-containing poly(1,8-octanediol-citrate) to a coupling reaction on a polydimethylsiloxane mould. The poly(1,8-octanediol-citrate) film modified through click chemistry has excellent hydrophilicity so that stem cells have better adhering performance for the film, thus greatly increasing the number of stem cells used for induced differentiation. In addition, a microenvironment of muscle tendon tissues is constructed on the film, thus effectively inducing differentiation of the stem cells into muscle tendon cells.

The invention provides a tissue culture and rapid propagation method of Jiuhua Polygonatum sibiricum. The tissue culture and rapid propagation method comprises the following step of: allocating and optimizing culture medium formulas and culture conditions of all culture phases by taking a Polygonatum sibiricum root-like stem as an explant through adventitious buds induction phase, callus propagation phase, root-taking phase and acclimatization and transplanting phase, so as to form a tissue culture technology which has a propagation speed of 106 times each year. The tissue culture and rapid propagation method realizes the tissue culture and the rapid and stable propagation of the Jiuhua Polygonatum sibiricum and can effectively solve the problem of high-quality seedling supply of the Jiuhua Polygonatum sibiricum.

The invention provides pluripotent human stem cell technology, method and product of small molecule oriented tissue and organ regeneration. The technology provided in the invention can directly and high-effectively convert pluripotent human stem cells into high-purity human cells in specific pedigree in large scale, which are high in quality, are clinical-grade and have no foreign matters, through small molecule oriented differentiation induction. The invention provides an approach of producing or manufacturing the pluripotent human stem cells, which are high in quality, are clinical-grade and have no foreign matters, and a functional human cell therapy derivative thereof in large scale for application of cell therapy and commercialization. In particular, the invention creatively provides a new commercial source of high-purity functional human neurons and myocardial cells for medical treatment of repairing central nervous system or cardiac muscle.

The invention relates to a 3D pseudo-skin construction method based on organs-on-chips and directional differentiation of induced pluripotent stem cells. According to the method, a 3D spinning supportis placed in sterilized organs-on-chips, fibroblast is inoculated on one surface of the 3D spinning support, keratinocyte is inoculated on the other surface of the 3D spinning support, then two culture mediums (CnT-07 epidermal keratinocyte medium and DMEM with 10 vol.% of FBS and 1 vol.% of penicillin-streptomycin) are introduced into the organs-on-chips to culture fibroblast and keratinocyte respectively, and the co-culture of fibroblast and keratinocyte and cell proliferation are realized. The construction method utilizes a stem cell differentiation technology to obtain required cells, thecell inoculation is performed on a nano structure, fibroblast and keratinocyte are co-cultured in organs-on-chips, and the method is used for epidermal tissue repair, is used to establish a drug model, and is the hot spot of research at present.

The present invention belongs to the field of stem cell biology, relates to lineage-specific differentiation of human multipotential or pluripotent stem cells, and particularly relates to a mesenchymal stem cell, and a preparation method and an application thereof. The method for preparing the mesenchymal stem cell comprises the following steps: S1, human multipotential stem cells form embryoid bodies; S2, the embryoid bodies differentiate into mesoderm cells; and S3, the mesodermal cells differentiate into the mesenchymal stem cells. The preparation method has a clear cell differentiation path, high differentiation efficiency, and stable differentiation effect and does not use serum-containing culture system or trophoblast cells, and the obtained cell population has high purity and largenumber, and thus is suitable for subsequent production and application of clinical-grade cell preparations.

The invention provides a culture medium for inducing differentiation of dental pulp stem cells into adipoblasts, a kit containing the culture medium and application of the kit. The culture medium comprises a basic culture medium, insulin, a platelet lysis buffer, indometacin, dexamethasone and 3-isobutyl-1-methylxanthine. The components contained in the culture medium have a good synergistic effect, so induced differentiation from the dental pulp stem cells to the adipocytes can be achieved, the adipogenic directional differentiation specificity of the culture medium is high, the specificity of the culture medium is high, time needed for the adipogenic induced differentiation of the dental pulp stem cells can be greatly shortened, and induced differentiation efficiency is improved. Meanwhile, the culture medium is applied to the kit for inducing the differentiation of the dental pulp stem cells into the adipoblasts. The kit is used for inducing the dental pulp stem cells, and stable and efficient induced differentiation of the dental pulp stem cells into the adipoblasts can be achieved.

The invention provides a screening method for IncRNAs which are in differential expression during chondrogenic differentiation of ADSCs, ADSCs with high cartilage differentiation capacity, and an induced differentiation method for cartilage cells. The screening method for IncRNAs comprises the following steps: gradient induced differentiation of ADSCs chondroblasts, IncRNAs sequencing of ADSCs, and analysis of IncRNAs sequencing results. The IncRNAs included in the ADSCs comprise at least one selected from the group consisting of H19, Scube3 and NONRATG00281. The ADSCs with high cartilage differentiation capacity are modified by the IncRNAs in differential expression.

The invention relates to the technical field of plant tissue culture, in particular to a method for regenerating prunus mume plants in a somatic embryo way and a special culture medium of the method. The invention provides the method for regenerating the prunus mume plants in the somatic embryo way. The method comprises the following steps: culturing prunus mume explants on a somatic embryo induction culture medium to obtain somatic embryos, and proliferating the somatic embryos on a somatic embryo proliferation culture medium to obtain secondary somatic embryos, and culturing the secondary somatic embryos in a somatic embryo germination culture medium to form plants. According to the method, the efficiency of generating the somatic embryos through induction of explants is high, the somatic embryos can be continuously proliferated in a secondary somatic embryo form, the proliferation effect is good, long-term subculture preservation can be achieved, the ability of the somatic embryos to be differentiated into plants can be kept, and the somatic embryos can be efficiently differentiated and regenerated into prunus mume plants after being cultured through the germination culture medium.

The invention relates to the technical field of gene engineering, in particular to a genetically modified stem cell and an application thereof. The genetically modified stem cell can express fusion protein Pdx-1-Linker-IGF-1 and be efficiently differentiated into islet-like cell clusters. An experiment shows a conversion rate after induction for 5 days can reach 90%, and an insulin secretory volume can reach 109-141 pg / mL. Compared with other groups, the genetically modified stem cell has a stronger islet cell transformation effect.

The invention relates to the field of stem cell biology, in particular to a method and application for differentiating human pluripotent stem cells into natural killer cells. The invention discloses a natural killer cell derived from pluripotent stem cells, which expresses CD56, Nkp30, Nkp44 and Nkp46, and also expresses markers CD16 and CD94 of mature natural killer cells. The invention also discloses a method for preparing natural killer cells, comprising the following steps: S1: forming embryoid bodies; S2: differentiation of embryoid bodies to hematopoietic progenitor cells; S3: differentiation of hematopoietic progenitor cells to NK cells; S4: NK cells maturation and expansion. The differentiation method provided by the present invention is based on a culture medium with defined components and an optimized combination of cytokines, which can rapidly, efficiently, simply and cost-effectively differentiate induced pluripotent stem cells into natural killer cells.

The invention discloses a method for inducing and amplifying decidua-like natural killer (NK) cells from hematopoietic stem cells in vitro. The method comprises the following steps: culturing CD34+ hematopoietic stem cells in four stages in vitro, namely amplifying the CD34+ hematopoietic stem cells in stage I, inducing differentiation of the CD34+ hematopoietic stem cells into NK cell precursors in stage II, inducing differentiation of the NK cell precursors into NK cells in stage III, inducing amplification and maturation of the NK cells and collecting CD3-CD56+NK cells in stage IV, and thus obtaining decidua-like natural killer cells, wherein the culture is started from a fresh basic culture medium containing a cytokine combination I, fresh basic culture mediums containing the cytokine combination I, combination II, combination III and combination IV are used in culture stages I, II, III and IV respectively for half-quantity liquid change culture, the cytokine combination I is composed of Flt3L and SCF; the combination II is composed of Flt3L, SCF and IL-15; the combination III is composed of Flt3L, SCF and IL-15; and the combination IV is IL-15.

The invention relates to a chondrogenic differentiation culture medium and application thereof. The chondrogenic differentiation culture medium is composed of a basic culture medium and additive components, and comprises the components of an inorganic salt component, an amino acid component, a vitamin component, a microelement component, carbohydrate, TGF-[beta]3, BMP-6, BMP-2, human serum albumin, dexamethasone, magnesium ascorbyl phosphate, recombinant human insulin, recombinant human transferrin, selenic acid and linoleic acid. The invention further provides application of the chondrogenicserum-free differentiation culture medium for mesenchymal stem cells. The chondrogenic differentiation culture medium is definite in component, free of animal-derived components and serum and safer, and the components can efficiently induce differentiation of the mesenchymal stem cells to chondrocytes under the synergistic effect.

The invention relates to a method for inducing pluripotent stem cells to be differentiated into melanocytes in a serum-free culture solution, which comprises the following steps: preparing a serum-free matrix culture solution of differentiated cells, including a No.1 culture medium, a No.2 culture medium and a DiffMel culture medium; culturing the pluripotent stem cells in the cell culture plate until the pluripotent stem cells overgrow 80% of the cell culture plate; inducing the pluripotent stem cells to be differentiated into precursor cells of melanocytes in a matrix culture solution; a culture dish for treatment and passage is coated, and precursor cells of the melanocytes are continuously induced to be differentiated into the melanocytes in a DiffMel culture medium; and culturing the differentiated melanocytes. The differentiated melanocytes have cell characteristics similar to those of human primary melanocytes, and have great difference from malignant melanoma cells. Compared with the prior art, the melanocyte differentiation method provided by the invention is short in time consumption and high in differentiation efficiency, and serum or other animal-derived preparations are not needed, so that a basis is provided for future clinical application.

The invention belongs to the technical field of cell culture, and particularly relates to application of SAFit2 and a culture medium in promoting differentiation of human induced pluripotent stem cells to dopaminergic neurons. The invention provides an application of SAFit2 in promoting efficient differentiation of human induced pluripotent stem cells to dopaminergic neurons. The SAFit2 provided by the invention can promote efficient differentiation of human induced pluripotent stem cells into dopaminergic neurons.

The invention relates to a method for constructing a 3D pseudo-epidermis based on organ chips and directed differentiation of induced pluripotent stem cells, comprising the following steps: placing a 3D spinning scaffold inoculated with fibroblasts on one side and keratinocytes on the other side in an inert environment. Into the organ chip after bacteria, two kinds of media, CnT-07 epidermal keratinocyte medium and DMEM (containing 10% volume ratio FBS, 1% volume ratio penicillin-streptomycin), were passed through to culture keratinocytes and fibroblasts respectively , to achieve co-culture and cell proliferation of keratinocytes and fibroblasts. In the construction method of the present invention, the required cells are obtained by using the stem cell differentiation technology, the cells are inoculated on the nanostructure, and the co-cultivation of keratinocytes and fibroblasts is realized in the organ chip, so that the repair of epidermal tissue has become a current research hotspot. and new methods for drug modeling.

The invention provides a method for obtaining pancreatic precursor cells and islet beta cells by differentiating human pluripotent stem cells. During the specialization process of endoderm cells derived from human pluripotent stem cells to pancreatic lineage cells, the differentiation efficiency of human pluripotent stem cells to pancreatic precursor cells can be improved by adding WNT signaling pathway inhibitors in stages. After improving the differentiation efficiency of pancreatic precursor cells, the invention further improves the differentiation efficiency of mature pancreatic beta cells. This method is suitable for pancreatic differentiation of different cell lines to obtain a large number of islet β cells, which provides strong support for diabetes cell therapy, drug screening, disease models, etc., and has a good application prospect.

The invention discloses a human early placenta development model establishing method based on organs-on-chips, which mainly comprises the following steps of pore plate modification and human pluripotent stem cell (hPSC) inoculation culture, cell digestion and inoculation into a three-dimensional matrix, human early placenta development model establishment and the like. A near-physiological three-dimensional cavity structure is formed in a three-dimensional matrix material by utilizing the self-assembly property of hPSC, and the cavities also show the characteristic of efficient differentiationof trophoblasts. The method has the characteristics of simplicity, easiness in operation, high repeatability and the like. The establishment of the model can provide a platform for exploring human placentogenesis and placentogenesis in the field of cell level and molecular mechanism research, and also has great application value in epigenetics research in the placental development process.

The invention discloses a new induction system and induction method for promoting chondrogenic differentiation of mesenchymal stem cells. The present invention optimizes the chondrogenic induction system of mesenchymal stem cells, and innovatively combines KGN pretreatment with traditional induction factor TGF-β3 to form a new chondrogenic differentiation induction system: the seed cells are first cultured in vitro After KGN pretreatment and TGF-β3 induction, mesenchymal stem cells can efficiently differentiate into chondrocytes, and the formed chondrocytes have a stable phenotype and no obvious ossification tendency. The invention significantly improves the chondrogenic differentiation efficiency of the mesenchymal stem cells, and can better play its role as seed cells for cartilage tissue engineering.