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A new induction system and induction method for promoting chondrogenic differentiation of mesenchymal stem cells

A technology of mesenchymal stem cells and differentiation induction, applied in the field of biomedicine, to achieve the effect of overcoming shortcomings, improving efficiency, and stabilizing chondrocyte phenotype

Active Publication Date: 2021-10-29
SHANGHAI CHILDRENS MEDICAL CENT AFFILIATED TO SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the prior art, there is no report about the new induction system and induction method of the present invention to promote the chondrogenic differentiation of mesenchymal stem cells.

Method used

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  • A new induction system and induction method for promoting chondrogenic differentiation of mesenchymal stem cells
  • A new induction system and induction method for promoting chondrogenic differentiation of mesenchymal stem cells
  • A new induction system and induction method for promoting chondrogenic differentiation of mesenchymal stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Novel Mesenchymal Stem Cell Chondrogenic Induction System (1)

[0029] A new type of mesenchymal stem cell chondrogenic induction system, comprising a first induction medium and a second induction medium, the first induction medium includes a common growth medium and a small molecule compound KGN, the concentration of the small molecule compound KGN is 1 μM, the common growth medium formula: α-MEM added with 5% serum replacement + 1% double antibody (penicillin / streptomycin); the second induction medium includes chondrogenic induction medium and TGF-β3 , the TGF-β3 concentration is 10ng / mL; the composition of the chondrogenic induction medium includes DMEM high glucose medium, 100nM dexamethasone, 50mg / mL ascorbic acid, 100mg / mL sodium pyruvate, 1% ITS.

Embodiment 2

[0030] Example 2 Novel Mesenchymal Stem Cell Chondrogenic Induction System (2)

[0031] A new type of mesenchymal stem cell chondrogenic induction system, comprising a first induction medium and a second induction medium, the first induction medium includes a common growth medium and a small molecule compound KGN, the concentration of the small molecule compound KGN is 3 μ M, the common growth medium formula: α-MEM added with 5% serum replacement + 1% double antibody (penicillin / streptomycin); the second induction medium includes chondrogenic induction medium and TGF-β3 , the TGF-β3 concentration is 1ng / mL; the composition of the chondrogenic induction medium includes DMEM high glucose medium, 100nM dexamethasone, 50mg / mL ascorbic acid, 100mg / mL sodium pyruvate, 1% ITS.

Embodiment 3

[0032] Example 3 Novel Mesenchymal Stem Cell Chondrogenic Induction System (3)

[0033] A new type of mesenchymal stem cell chondrogenic induction system, comprising a first induction medium and a second induction medium, the first induction medium includes a common growth medium and a small molecule compound KGN, the concentration of the small molecule compound KGN is 5 μ M, the common growth medium formula: α-MEM added 5% serum replacement + 1% double antibody (penicillin / streptomycin); the second induction medium includes chondrogenic induction medium and TGF-β3 , the TGF-β3 concentration is 5ng / mL; the composition of the chondrogenic induction medium includes DMEM high glucose medium, 100nM dexamethasone, 50mg / mL ascorbic acid, 100mg / mL sodium pyruvate, 1% ITS.

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Abstract

The invention discloses a new induction system and induction method for promoting chondrogenic differentiation of mesenchymal stem cells. The present invention optimizes the chondrogenic induction system of mesenchymal stem cells, and innovatively combines KGN pretreatment with traditional induction factor TGF-β3 to form a new chondrogenic differentiation induction system: the seed cells are first cultured in vitro After KGN pretreatment and TGF-β3 induction, mesenchymal stem cells can efficiently differentiate into chondrocytes, and the formed chondrocytes have a stable phenotype and no obvious ossification tendency. The invention significantly improves the chondrogenic differentiation efficiency of the mesenchymal stem cells, and can better play its role as seed cells for cartilage tissue engineering.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a new induction system and induction method for promoting chondrogenic differentiation of mesenchymal stem cells. Background technique [0002] Trauma, disease, or congenital deformity often cause cartilage tissues such as tracheal cartilage and articular cartilage to be damaged or forced to be removed, but cartilage lacks regenerative ability, and it is difficult to repair itself after damage, so other tissues or substitute materials must be used for repair and reconstruction . However, the sources of autologous cartilage tissue are limited, and allogeneic cartilage tissue or artificial materials have problems such as immune rejection or lack of growth potential, which seriously affect the therapeutic effect and the quality of life of patients. With the emergence and continuous development of tissue engineering technology, cartilage tissue engineering provides a new opportu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077
CPCC12N5/0655C12N2500/30C12N2501/15C12N2506/1369
Inventor 何晓敏郑景浩荆辉张晓阳罗凯祝忠群
Owner SHANGHAI CHILDRENS MEDICAL CENT AFFILIATED TO SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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