3 results about "Cartilaginous Differentiation" patented technology

The application discloses a kind of biomimetic composite scaffold with periosteum-cortical bone-cancellous bone structure and its preparation method and application.The scaffold is assembled by independent periosteum biomimetic scaffold module and independent bone trunk biomimetic module.The periosteum biomimetic scaffold module is cylindrical tube structure hydrogel, loaded with zinc silicate particles and periosteum related cells, with the biological functions of promoting cartilage differentiation, secreting nerve and vascular factors and synthesizing periosteum matrix proteins.The bone trunk biomimetic scaffold module is cylindrical bioceramic, with multiple vertical pipes embedded in the dense layer of outer wall to simulate cortical bone, and with staggered structure inside to simulate cancellous bone.Animal experiments show that the biomimetic composite scaffold can effectively promote the integrated regeneration of bone trunk and periosteum in rabbit radial critical size defect model.Therefore, the application provides a new solution for the biomimetic design and regeneration repair of bone tissue engineering.

Use of a milk fruit extract for the preparation of a composition for improving bone quality and promoting chondrocyte differentiation. The milk fruit extract is extracted from early-harvested fruits of Chrysophyllum cainito using water as a solvent.

The application relates to the technical field of mesenchymal stromal cells, in particular to a method for detecting the chondrogenic differentiation capacity of mesenchymal stromal cells, a method for screening mesenchymal stromal cells and application. The method comprises the following steps: culturing mesenchymal stromal cells of any generation from the third to the seventh generation in three dimensions, detecting the contents of COMP and TSP2 in the supernatant of the cells after the culture, and detecting the chondrogenic differentiation capacity of the mesenchymal stromal cells or screening the mesenchymal stromal cells according to the contents of COMP and TSP2. The application solves the technical problems that the mesenchymal stromal cells are highly heterogeneous, the chondrogenic differentiation detection method is unstable, and there is a lack of objective and quantifiable judgment standard in the prior art, and provides a standardized, scaled and high-accuracy cell quality control method and high-quality cell screening scheme for the field of cartilage regeneration.