4 results about "Mesenchymal stem cell proliferation" patented technology

Therefore, this disclosure provides a genetically engineered mesenchymal stem cell (MSC) population, including an expression vector containing the Akt or HGF gene and the PD-L1 gene. It also provides a method for synergistically improving the survival status and immunomodulatory capacity of mesenchymal stem cells or enhancing their proliferation, including transfecting mesenchymal stem cells with the Akt or HGF gene and the PD-L1 gene; and a method for preventing, improving, and / or treating ischemic conditions, enhancing neurogenesis, or reducing neuronal death, including administering an effective amount of the genetically engineered mesenchymal stem cell population of this disclosure to individuals in need.

The invention discloses application of an extract in proliferation and differentiation of mesenchymal stem cells, and relates to the technical field of biological medicines. The invention specifically discloses perilla frutescens, black sesame, lophatherum gracile, lotus leaves, mulberry leaves, houttuynia cordata, angelica dahurica, smoked plum, fresh lalang grass rhizome, coix seeds, endive, fructus alpiniae oxyphyllae, astragalus membranaceus, dandelion fruits, chrysanthemum, dandelion, citron, gardenia, fresh rhizoma phragmitis, orange peel, semen raphani, mint, perilla seeds, fermented soybean, fructus cannabis, sealwort, agkistrodon halys, brassica juncea, red beans, red dates, hawthorn, bunge cherry seeds, raspberry, Chinese torreya, ginseng and the root of kudzu vine. The extract of at least one of semen ziziphi spinosae, exocarpium citri rubrum, black pepper, platycodon grandiflorum, emblic leafflower fruit, longan, hovenia dulcis thunb, agastache rugosus, Chinese olive, momordica grosvenori, semen cassiae, fingered citron, eucommia ulmoides, galangal, medlar and white hyacinth bean can regulate and control proliferation of the mesenchymal stem cells, and meanwhile, the multidirectional differentiation potential of the mesenchymal stem cells can be effectively maintained.

The invention belongs to the technical field of exosome application, discloses a preparation method and application of an exosome for promoting hair regeneration, and finds a traditional Chinese medicine composition which can be used for promoting proliferation of mesenchymal stem cells and promoting improvement of dryness of the mesenchymal stem cells. Besides, after the traditional Chinese medicine composition is used for intragastric administration of animals, serum (hereinafter referred to as drug-containing serum) containing the traditional Chinese medicine composition is generated, the process of taking traditional Chinese medicine by a human body is simulated, and after the generated drug-containing serum and the mesenchymal stem cells are co-incubated, the mesenchymal stem cell exosome obtained through separation can be used for preparing the mesenchymal stem cell exosome. Results show that the inductivity of the exosome to mesenchymal stem cells is enhanced, and compared with normal exosomes, the separated exosome has a better effect of promoting hair regeneration.

The application discloses application of Phascolosoma esculenta exosome in preparation of a preparation for promoting osteogenic differentiation of bone marrow mesenchymal stem cells, and belongs to the technical field of marine biological medical materials. Fresh and live Phascolosoma esculenta is used as raw material, body cavity fluid is collected, and Phascolosoma esculenta exosome is obtained through separation and purification by means of fractional filtration, differential centrifugation and ultracentrifugation. The exosome has uniform particle size, high biocompatibility and no cytotoxicity, can significantly promote proliferation of human bone marrow mesenchymal stem cells in a low concentration range of 5-10 μg / mL, up-regulate expression levels of ALP, RUNX2 and OPN osteogenic marker proteins, improve alkaline phosphatase activity, promote calcium nodule deposition, accelerate osteogenic differentiation of stem cells, and has good biological medicine development prospect and industrial application value.