9 results about "Mesenchymal stem cell proliferation" patented technology

Therefore, this disclosure provides a genetically engineered mesenchymal stem cell (MSC) population, including an expression vector containing the Akt or HGF gene and the PD-L1 gene. It also provides a method for synergistically improving the survival status and immunomodulatory capacity of mesenchymal stem cells or enhancing their proliferation, including transfecting mesenchymal stem cells with the Akt or HGF gene and the PD-L1 gene; and a method for preventing, improving, and / or treating ischemic conditions, enhancing neurogenesis, or reducing neuronal death, including administering an effective amount of the genetically engineered mesenchymal stem cell population of this disclosure to individuals in need.

The invention provides a serum-free medium for maintaining dryness of mesenchymal stem cells as well as a preparation method and application of the serum-free medium, and belongs to the technical field of biological medicines. The serum-free culture medium comprises a basic culture medium and an exogenous additive component, the basic culture medium is a DMEM / F12 culture medium, and the exogenous additive components comprise bFGF, EGF, PDGF-BB, recombinant human fibronectin, laminin, insulin, transferrin, sodium selenate, beta-mercaptoethanol and ascorbic acid. The serum-free culture medium provided by the invention is a special culture medium for the mesenchymal stem cells, which is completely free of animal-derived components, is suitable for large-scale amplification of clinical-grade cell treatment products, is relatively low in preparation cost and high in stem differentiation capability, and can remarkably improve the proliferation efficiency of the cultured mesenchymal stem cells.

The invention discloses a culture solution for inducing proliferation and differentiation of mesenchymal stem cells and a preparation method of the culture solution, the culture solution comprises the following raw materials in parts by weight: 40-60 parts of a basal culture medium, 5-10 parts of a serum substitute, 3-5 parts of growth factors, 1-3 parts of hormones, 1-3 parts of vitamins, 0.5-1 part of a small molecule compound and 1-3 parts of other additives, and the invention relates to the technical field of stem cell culture. According to the culture solution for inducing proliferation and differentiation of the mesenchymal stem cells and the preparation method of the culture solution, by optimizing basic components and adding specific growth factors and small molecule compounds, the proliferation capacity and directional differentiation efficiency of the MSCs are remarkably improved, and the culture solution is suitable for in-vitro induction of various tissues such as bones, cartilages and nerves. The culture solution adopts an animal-origin-free formula, has the advantages of low cost, high safety, strong applicability and the like, and provides an important technical tool for the field of regenerative medicine.

The invention discloses a method for large-scale acquisition of mesenchymal stem cell exosomes with high expression of IL-10 and application of the mesenchymal stem cell exosomes, and relates to the technical field of umbilical cord mesenchymal stem cell exosomes. The method comprises the following steps: carrying out cross culture on umbilical cord mesenchymal stem cells by adopting complete culture and hunger culture, and collecting a solution discharged from a cell factory when the hunger culture is finished each time, so as to obtain an exosome stock solution; and filtering the exosome stock solution to remove impurities, so as to obtain the mesenchymal stem cell exosome with high expression of IL-10. According to the method, proliferation of the umbilical cord mesenchymal stem cells can be effectively inhibited through alternate culture of the starvation culture medium and the complete culture medium, exosome secretion is stimulated, and high-yield, high-efficiency and stable exosome production is achieved. The mesenchymal stem cell exosome with high expression of IL-10 is applied to clinical treatment of HFpEF, has an anti-apoptosis protection effect on myocardial cells, and effectively promotes angiogenesis.

The invention discloses application of an extract in proliferation and differentiation of mesenchymal stem cells, and relates to the technical field of biological medicines. The invention specifically discloses perilla frutescens, black sesame, lophatherum gracile, lotus leaves, mulberry leaves, houttuynia cordata, angelica dahurica, smoked plum, fresh lalang grass rhizome, coix seeds, endive, fructus alpiniae oxyphyllae, astragalus membranaceus, dandelion fruits, chrysanthemum, dandelion, citron, gardenia, fresh rhizoma phragmitis, orange peel, semen raphani, mint, perilla seeds, fermented soybean, fructus cannabis, sealwort, agkistrodon halys, brassica juncea, red beans, red dates, hawthorn, bunge cherry seeds, raspberry, Chinese torreya, ginseng and the root of kudzu vine. The extract of at least one of semen ziziphi spinosae, exocarpium citri rubrum, black pepper, platycodon grandiflorum, emblic leafflower fruit, longan, hovenia dulcis thunb, agastache rugosus, Chinese olive, momordica grosvenori, semen cassiae, fingered citron, eucommia ulmoides, galangal, medlar and white hyacinth bean can regulate and control proliferation of the mesenchymal stem cells, and meanwhile, the multidirectional differentiation potential of the mesenchymal stem cells can be effectively maintained.

The purpose of the present invention is to provide a culture medium for culturing having excellent proliferation of mesenchymal stem cells. The present invention is a mesenchymal stem cell proliferation promoter containing 16: 0 lysophosphatidic acid, a derivative of 16: 0 lysophosphatidic acid, and / or a salt of the 16: 0 lysophosphatidic acid and / or the derivative of 16: 0 lysophosphatidic acid. It is preferable that the mesenchymal stem cell proliferation promoter additionally contains 18: 1 lysophosphatidic acid, a derivative of 18: 1 lysophosphatidic acid, and / or a salt of the 18: 1 lysophosphatidic acid and / or the derivative of 18: 1 lysophosphatidic acid. The present invention also includes a culture medium for mesenchymal stem cells containing 16: 0 lysophosphatidic acid, a derivative of 16: 0 lysophosphatidic acid, and / or salts of the 16: 0 lysophosphatidic acid and the derivative of 16: 0 lysophosphatidic acid.

The invention belongs to the technical field of cell culture, and particularly relates to application of isorhamnetin in preparation of a culture medium for promoting proliferation of mesenchymal stem cells. The concentration of isorhamnetin in the culture medium is 10-20 [mu] M. The culture medium for promoting proliferation of the mesenchymal stem cells, provided by the invention, is a growth culture medium added with a natural flavonoid compound isorhamnetin, proliferation of the adipose-derived mesenchymal stem cells can be remarkably promoted by adopting the improved culture medium for culture, and the requirement of cultured meat production on the number of seed cells is well met.

The invention belongs to the technical field of exosome application, discloses a preparation method and application of an exosome for promoting hair regeneration, and finds a traditional Chinese medicine composition which can be used for promoting proliferation of mesenchymal stem cells and promoting improvement of dryness of the mesenchymal stem cells. Besides, after the traditional Chinese medicine composition is used for intragastric administration of animals, serum (hereinafter referred to as drug-containing serum) containing the traditional Chinese medicine composition is generated, the process of taking traditional Chinese medicine by a human body is simulated, and after the generated drug-containing serum and the mesenchymal stem cells are co-incubated, the mesenchymal stem cell exosome obtained through separation can be used for preparing the mesenchymal stem cell exosome. Results show that the inductivity of the exosome to mesenchymal stem cells is enhanced, and compared with normal exosomes, the separated exosome has a better effect of promoting hair regeneration.

The application discloses application of Phascolosoma esculenta exosome in preparation of a preparation for promoting osteogenic differentiation of bone marrow mesenchymal stem cells, and belongs to the technical field of marine biological medical materials. Fresh and live Phascolosoma esculenta is used as raw material, body cavity fluid is collected, and Phascolosoma esculenta exosome is obtained through separation and purification by means of fractional filtration, differential centrifugation and ultracentrifugation. The exosome has uniform particle size, high biocompatibility and no cytotoxicity, can significantly promote proliferation of human bone marrow mesenchymal stem cells in a low concentration range of 5-10 μg / mL, up-regulate expression levels of ALP, RUNX2 and OPN osteogenic marker proteins, improve alkaline phosphatase activity, promote calcium nodule deposition, accelerate osteogenic differentiation of stem cells, and has good biological medicine development prospect and industrial application value.