18 results about "Mesenchymal stem cell proliferation" patented technology

The invention belongs to the technical field of biology, and particularly relates to polypeptide for promoting proliferation of mesenchymal stem cells and application of the polypeptide. The amino acid sequence of the polypeptide for promoting proliferation of the mesenchymal stem cells is shown as SEQ ID NO: 1. The active polypeptide for promoting proliferation of the umbilical cord mesenchymal stem cells is obtained by adopting a solid-phase synthesis method. Umbilical cord mesenchymal stem cells obtained by culturing the polypeptide have good adherence and multiplication capacities. In addition, the polypeptide can promote Nrf2 gene expression, so that proliferation of the umbilical cord mesenchymal stem cells is effectively promoted.

Therefore, this disclosure provides a genetically engineered mesenchymal stem cell (MSC) population, including an expression vector containing the Akt or HGF gene and the PD-L1 gene. It also provides a method for synergistically improving the survival status and immunomodulatory capacity of mesenchymal stem cells or enhancing their proliferation, including transfecting mesenchymal stem cells with the Akt or HGF gene and the PD-L1 gene; and a method for preventing, improving, and / or treating ischemic conditions, enhancing neurogenesis, or reducing neuronal death, including administering an effective amount of the genetically engineered mesenchymal stem cell population of this disclosure to individuals in need.

The invention discloses an effect of deuterium-depleted water on continuous growth of human mesenchymal stem cells, and belongs to the field of biomedicine. The invention relates to a medicine for promoting the growth of human mesenchymal stem cells. The medicine comprises deuterium-depleted water with the concentration of 25-50 ppm. On the other hand, the invention further relates to application of the deuterium-depleted water in preparation of drugs for promoting growth of human mesenchymal stem cells. Compared with the prior art, the invention discloses the effect of the deuterium-depleted water on the human mesenchymal stem cells, specifically, the deuterium-depleted water is used for improving the proliferation capacity or proliferation activity of the human mesenchymal stem cells, and the deuterium-depleted water is used for preparing the deuterium-depleted water for prolonging the life of the human mesenchymal stem cells and promoting the single cell clone formation capacity of the human mesenchymal stem cells. And a new research and development direction is provided for repairing and culturing human mesenchymal stem cells.

The invention provides a composition for promoting proliferation of mesenchymal stem cells and / or relieving spermatogonial cell injury as well as a preparation method and application of the composition, and belongs to the technical field of traditional Chinese medicine compositions. The composition is prepared from the following raw materials in parts by weight: 10-20 parts of ginseng, 5-15 parts of maca, 5-10 parts of oyster peptide, 5-15 parts of sika deer penis, 3-8 parts of sika deer blood, 3-8 parts of fresh ginger, 5-8 parts of herba cistanche, 4-10 parts of sea cucumber peptide, 4-8 parts of rhizoma polygonati, 5-10 parts of burdock root, 2-5 parts of fructus lycii powder and 1-3 parts of folium cortex eucommiae. The composition is prepared into a traditional Chinese medicine preparation by adopting a superfine grinding process, and the traditional Chinese medicine preparation has the effects of promoting proliferation of mesenchymal stem cells (MSCs), repairing spermatogonial cell injury caused by oxidative stress (such as chemotherapy and radiation) or aging (such as age-related), reducing the ROS level, improving spermatogenic dysfunction, assisting in treating male infertility and delaying senescence of a reproductive system and a whole body.

An induction medium for increasing the IL-10 secretion of mesenchymal stem cells and its application, belonging to the technical field of stem cell culture. In order to provide a method that can both increase the IL-10 secretion of mesenchymal stem cells and not affect the proliferation of mesenchymal stem cells, the present invention uses a basal medium containing human IFN-γ protein and TNF-α mimetic peptide to induce the amplified human mesenchymal cells, and then after harvesting and culturing, mesenchymal stem cells with increased IL-10 secretion are obtained. The induction medium provided by the present invention can increase the IL-10 secretion of mesenchymal stem cells while not affecting the proliferation of mesenchymal stem cells, and the induced mesenchymal stem cells are expected to be applied to the preparation of cell drugs for treating inflammatory diseases.

The invention provides a serum-free medium for maintaining dryness of mesenchymal stem cells as well as a preparation method and application of the serum-free medium, and belongs to the technical field of biological medicines. The serum-free culture medium comprises a basic culture medium and an exogenous additive component, the basic culture medium is a DMEM / F12 culture medium, and the exogenous additive components comprise bFGF, EGF, PDGF-BB, recombinant human fibronectin, laminin, insulin, transferrin, sodium selenate, beta-mercaptoethanol and ascorbic acid. The serum-free culture medium provided by the invention is a special culture medium for the mesenchymal stem cells, which is completely free of animal-derived components, is suitable for large-scale amplification of clinical-grade cell treatment products, is relatively low in preparation cost and high in stem differentiation capability, and can remarkably improve the proliferation efficiency of the cultured mesenchymal stem cells.

The invention discloses a culture solution for inducing proliferation and differentiation of mesenchymal stem cells and a preparation method of the culture solution, the culture solution comprises the following raw materials in parts by weight: 40-60 parts of a basal culture medium, 5-10 parts of a serum substitute, 3-5 parts of growth factors, 1-3 parts of hormones, 1-3 parts of vitamins, 0.5-1 part of a small molecule compound and 1-3 parts of other additives, and the invention relates to the technical field of stem cell culture. According to the culture solution for inducing proliferation and differentiation of the mesenchymal stem cells and the preparation method of the culture solution, by optimizing basic components and adding specific growth factors and small molecule compounds, the proliferation capacity and directional differentiation efficiency of the MSCs are remarkably improved, and the culture solution is suitable for in-vitro induction of various tissues such as bones, cartilages and nerves. The culture solution adopts an animal-origin-free formula, has the advantages of low cost, high safety, strong applicability and the like, and provides an important technical tool for the field of regenerative medicine.

The invention discloses a method for large-scale acquisition of mesenchymal stem cell exosomes with high expression of IL-10 and application of the mesenchymal stem cell exosomes, and relates to the technical field of umbilical cord mesenchymal stem cell exosomes. The method comprises the following steps: carrying out cross culture on umbilical cord mesenchymal stem cells by adopting complete culture and hunger culture, and collecting a solution discharged from a cell factory when the hunger culture is finished each time, so as to obtain an exosome stock solution; and filtering the exosome stock solution to remove impurities, so as to obtain the mesenchymal stem cell exosome with high expression of IL-10. According to the method, proliferation of the umbilical cord mesenchymal stem cells can be effectively inhibited through alternate culture of the starvation culture medium and the complete culture medium, exosome secretion is stimulated, and high-yield, high-efficiency and stable exosome production is achieved. The mesenchymal stem cell exosome with high expression of IL-10 is applied to clinical treatment of HFpEF, has an anti-apoptosis protection effect on myocardial cells, and effectively promotes angiogenesis.

The application discloses a modified polyether ketone ketone composite material and a preparation method and application thereof, and relates to the technical field of composite materials, in particular to a modified polyether ketone ketone composite material, which comprises a sulfonated porous composite support, a chemotactic factor and a methacrylamide crystal glue; the preparation raw material of the sulfonated porous composite support comprises polyether ketone ketone and strontium-cerium doped bioactive glass; the chemotactic factor and the methacrylamide crystal glue are loaded in the pores of the sulfonated porous composite support. The three-dimensional porous composite support prepared by adopting PEKK and BG is used as the main body of the composite material, the chemotactic factor and the GelMA crystal glue are loaded in the pores, so that the immune regulation characteristics and the bone induction property of the PEKK-based support are enhanced; the prepared modified polyether ketone ketone composite material can significantly promote the proliferation and osteogenic differentiation of mesenchymal stem cells, is beneficial to the recruitment and polarization of macrophages, has a good bone formation effect in the immune-bone cascade reaction, and can accelerate the osteogenic differentiation process of the mesenchymal stem cells after being co-cultured with the macrophages.

The invention discloses application of an extract in proliferation and differentiation of mesenchymal stem cells, and relates to the technical field of biological medicines. The invention specifically discloses perilla frutescens, black sesame, lophatherum gracile, lotus leaves, mulberry leaves, houttuynia cordata, angelica dahurica, smoked plum, fresh lalang grass rhizome, coix seeds, endive, fructus alpiniae oxyphyllae, astragalus membranaceus, dandelion fruits, chrysanthemum, dandelion, citron, gardenia, fresh rhizoma phragmitis, orange peel, semen raphani, mint, perilla seeds, fermented soybean, fructus cannabis, sealwort, agkistrodon halys, brassica juncea, red beans, red dates, hawthorn, bunge cherry seeds, raspberry, Chinese torreya, ginseng and the root of kudzu vine. The extract of at least one of semen ziziphi spinosae, exocarpium citri rubrum, black pepper, platycodon grandiflorum, emblic leafflower fruit, longan, hovenia dulcis thunb, agastache rugosus, Chinese olive, momordica grosvenori, semen cassiae, fingered citron, eucommia ulmoides, galangal, medlar and white hyacinth bean can regulate and control proliferation of the mesenchymal stem cells, and meanwhile, the multidirectional differentiation potential of the mesenchymal stem cells can be effectively maintained.

The invention discloses preparation and new application of growth hormone releasing hormone similar peptide monomers, dimers and tetramers, which can regulate the synergistic expression of human organ autocrine GHRH receptor down and GHSR up and down, directly promote the proliferation and / or activation of organ mesenchymal stem cells, and can be used for preparing medicines for treating organ injury, protecting diseases, and preventing and treating diseases. The compounds can be used as anti-aging, organ injury protection, cancer chemotherapy protection, anti-cancer drugs and the like.

The purpose of the present invention is to provide a culture medium for culturing having excellent proliferation of mesenchymal stem cells. The present invention is a mesenchymal stem cell proliferation promoter containing 16: 0 lysophosphatidic acid, a derivative of 16: 0 lysophosphatidic acid, and / or a salt of the 16: 0 lysophosphatidic acid and / or the derivative of 16: 0 lysophosphatidic acid. It is preferable that the mesenchymal stem cell proliferation promoter additionally contains 18: 1 lysophosphatidic acid, a derivative of 18: 1 lysophosphatidic acid, and / or a salt of the 18: 1 lysophosphatidic acid and / or the derivative of 18: 1 lysophosphatidic acid. The present invention also includes a culture medium for mesenchymal stem cells containing 16: 0 lysophosphatidic acid, a derivative of 16: 0 lysophosphatidic acid, and / or salts of the 16: 0 lysophosphatidic acid and the derivative of 16: 0 lysophosphatidic acid.

The invention belongs to the technical field of culture of mesenchymal stem cells, and particularly relates to a serum-free mesenchymal stem cell culture medium and a preparation method thereof. The culture medium comprises a basic culture medium and exogenous supplementary factors, wherein the exogenous supplementary factors comprise oligosaccharide peptide, highland barley fermentation liquor and multiflora rose glycoside; wherein the concentration of the oligosaccharide peptide in the basal culture medium is 10-50 [mu] g / mL, the concentration of the highland barley fermentation liquor in the basal culture medium is 30-80 [mu] g / mL, and the concentration of the multiflora rose glycoside in the basal culture medium is 1-5 [mu] g / mL. According to the culture medium disclosed by the invention, the oligosaccharide peptide, the highland barley fermentation liquor and the multiflora rose glycoside are added on the basis of the original basic culture medium, and the components are reasonably matched, so that the mesenchymal stem cells, particularly the proliferation and activity of the adipose-derived mesenchymal stem cells can be favorably maintained and stimulated; better proliferative activity and survival rate are shown.

The invention belongs to the technical field of cell culture, and particularly relates to application of isorhamnetin in preparation of a culture medium for promoting proliferation of mesenchymal stem cells. The concentration of isorhamnetin in the culture medium is 10-20 [mu] M. The culture medium for promoting proliferation of the mesenchymal stem cells, provided by the invention, is a growth culture medium added with a natural flavonoid compound isorhamnetin, proliferation of the adipose-derived mesenchymal stem cells can be remarkably promoted by adopting the improved culture medium for culture, and the requirement of cultured meat production on the number of seed cells is well met.

The invention belongs to the technical field of exosome application, discloses a preparation method and application of an exosome for promoting hair regeneration, and finds a traditional Chinese medicine composition which can be used for promoting proliferation of mesenchymal stem cells and promoting improvement of dryness of the mesenchymal stem cells. Besides, after the traditional Chinese medicine composition is used for intragastric administration of animals, serum (hereinafter referred to as drug-containing serum) containing the traditional Chinese medicine composition is generated, the process of taking traditional Chinese medicine by a human body is simulated, and after the generated drug-containing serum and the mesenchymal stem cells are co-incubated, the mesenchymal stem cell exosome obtained through separation can be used for preparing the mesenchymal stem cell exosome. Results show that the inductivity of the exosome to mesenchymal stem cells is enhanced, and compared with normal exosomes, the separated exosome has a better effect of promoting hair regeneration.

The application discloses application of Phascolosoma esculenta exosome in preparation of a preparation for promoting osteogenic differentiation of bone marrow mesenchymal stem cells, and belongs to the technical field of marine biological medical materials. Fresh and live Phascolosoma esculenta is used as raw material, body cavity fluid is collected, and Phascolosoma esculenta exosome is obtained through separation and purification by means of fractional filtration, differential centrifugation and ultracentrifugation. The exosome has uniform particle size, high biocompatibility and no cytotoxicity, can significantly promote proliferation of human bone marrow mesenchymal stem cells in a low concentration range of 5-10 μg / mL, up-regulate expression levels of ALP, RUNX2 and OPN osteogenic marker proteins, improve alkaline phosphatase activity, promote calcium nodule deposition, accelerate osteogenic differentiation of stem cells, and has good biological medicine development prospect and industrial application value.

The application belongs to the technical field of biology and particularly relates to a polypeptide for promoting mesenchymal stem cell proliferation and application thereof. The amino acid sequence of the polypeptide for promoting mesenchymal stem cell proliferation is shown as SEQ ID NO:1. The polypeptide for promoting umbilical cord mesenchymal stem cell proliferation is obtained by using a solid-phase synthesis method. The umbilical cord mesenchymal stem cell obtained by using the polypeptide for culture has good adhesion and proliferation capacity. In addition, the polypeptide can also promote Nrf2 gene expression, thereby effectively promoting the proliferation of umbilical cord mesenchymal stem cells.

The present invention relates to the technical field of stem cell detection, and particularly to a detection method based on human stem cells. The method includes preprocessing the acquired image data of the stem cell culture process to obtain analyzable image data; determining a prediction method for the number of stem cells based on the fluctuation degree of the proliferation rate of mesenchymal stem cells in a plurality of analyzable image data; determining to replace the culture medium or divide the mesenchymal stem cells into a new culture dish based on the proportion of the increased volume of mesenchymal stem cells and whether the mesenchymal stem cells show a multi-layer growth phenomenon under the condition that the deviation value is greater than a preset deviation value; determining to adjust the preset fluctuation degree or adjust the preset uniform distribution degree based on the occurrence frequency of the deviation value between the actual number and the predicted number of mesenchymal stem cells being greater than the preset deviation value under each prediction method after corresponding adjustment within a preset time period, and whether the image data corresponding to the occurrence time point has been preprocessed.