26 results about "Mesenchymal stem cell proliferation" patented technology

The invention belongs to the technical field of biology, and particularly relates to polypeptide for promoting proliferation of mesenchymal stem cells and application of the polypeptide. The amino acid sequence of the polypeptide for promoting proliferation of the mesenchymal stem cells is shown as SEQ ID NO: 1. The active polypeptide for promoting proliferation of the umbilical cord mesenchymal stem cells is obtained by adopting a solid-phase synthesis method. Umbilical cord mesenchymal stem cells obtained by culturing the polypeptide have good adherence and multiplication capacities. In addition, the polypeptide can promote Nrf2 gene expression, so that proliferation of the umbilical cord mesenchymal stem cells is effectively promoted.

The invention provides a culture method for improving proliferation activity and factor secretion of mesenchymal stem cells, and belongs to the technical field of cell culture, the mesenchymal stem cells are cultured in a low-oxygen environment, and the continuous proliferation, division and clone formation capabilities of the mesenchymal stem cells are improved through low-oxygen induction culture, so that the proliferation activity and factor secretion of the mesenchymal stem cells are improved. Compared with the prior art, the method has the advantages that the expression of the mesenchymal stem cells on pluripotent related genes, angiogenesis related genes and immunoregulation related genes is improved, the secretion amount of related factors is increased, the specific surface marker genes of the mesenchymal stem cells are not influenced, and the application prospect is wide. And the heterogeneity and the homogeneity of the cell population are not influenced.

Therefore, this disclosure provides a genetically engineered mesenchymal stem cell (MSC) population, including an expression vector containing the Akt or HGF gene and the PD-L1 gene. It also provides a method for synergistically improving the survival status and immunomodulatory capacity of mesenchymal stem cells or enhancing their proliferation, including transfecting mesenchymal stem cells with the Akt or HGF gene and the PD-L1 gene; and a method for preventing, improving, and / or treating ischemic conditions, enhancing neurogenesis, or reducing neuronal death, including administering an effective amount of the genetically engineered mesenchymal stem cell population of this disclosure to individuals in need.

The invention discloses an effect of deuterium-depleted water on continuous growth of human mesenchymal stem cells, and belongs to the field of biomedicine. The invention relates to a medicine for promoting the growth of human mesenchymal stem cells. The medicine comprises deuterium-depleted water with the concentration of 25-50 ppm. On the other hand, the invention further relates to application of the deuterium-depleted water in preparation of drugs for promoting growth of human mesenchymal stem cells. Compared with the prior art, the invention discloses the effect of the deuterium-depleted water on the human mesenchymal stem cells, specifically, the deuterium-depleted water is used for improving the proliferation capacity or proliferation activity of the human mesenchymal stem cells, and the deuterium-depleted water is used for preparing the deuterium-depleted water for prolonging the life of the human mesenchymal stem cells and promoting the single cell clone formation capacity of the human mesenchymal stem cells. And a new research and development direction is provided for repairing and culturing human mesenchymal stem cells.

The invention provides a composition for promoting proliferation of mesenchymal stem cells and / or relieving spermatogonial cell injury as well as a preparation method and application of the composition, and belongs to the technical field of traditional Chinese medicine compositions. The composition is prepared from the following raw materials in parts by weight: 10-20 parts of ginseng, 5-15 parts of maca, 5-10 parts of oyster peptide, 5-15 parts of sika deer penis, 3-8 parts of sika deer blood, 3-8 parts of fresh ginger, 5-8 parts of herba cistanche, 4-10 parts of sea cucumber peptide, 4-8 parts of rhizoma polygonati, 5-10 parts of burdock root, 2-5 parts of fructus lycii powder and 1-3 parts of folium cortex eucommiae. The composition is prepared into a traditional Chinese medicine preparation by adopting a superfine grinding process, and the traditional Chinese medicine preparation has the effects of promoting proliferation of mesenchymal stem cells (MSCs), repairing spermatogonial cell injury caused by oxidative stress (such as chemotherapy and radiation) or aging (such as age-related), reducing the ROS level, improving spermatogenic dysfunction, assisting in treating male infertility and delaying senescence of a reproductive system and a whole body.

An induction medium for increasing the IL-10 secretion of mesenchymal stem cells and its application, belonging to the technical field of stem cell culture. In order to provide a method that can both increase the IL-10 secretion of mesenchymal stem cells and not affect the proliferation of mesenchymal stem cells, the present invention uses a basal medium containing human IFN-γ protein and TNF-α mimetic peptide to induce the amplified human mesenchymal cells, and then after harvesting and culturing, mesenchymal stem cells with increased IL-10 secretion are obtained. The induction medium provided by the present invention can increase the IL-10 secretion of mesenchymal stem cells while not affecting the proliferation of mesenchymal stem cells, and the induced mesenchymal stem cells are expected to be applied to the preparation of cell drugs for treating inflammatory diseases.

The invention provides a serum-free medium for maintaining dryness of mesenchymal stem cells as well as a preparation method and application of the serum-free medium, and belongs to the technical field of biological medicines. The serum-free culture medium comprises a basic culture medium and an exogenous additive component, the basic culture medium is a DMEM / F12 culture medium, and the exogenous additive components comprise bFGF, EGF, PDGF-BB, recombinant human fibronectin, laminin, insulin, transferrin, sodium selenate, beta-mercaptoethanol and ascorbic acid. The serum-free culture medium provided by the invention is a special culture medium for the mesenchymal stem cells, which is completely free of animal-derived components, is suitable for large-scale amplification of clinical-grade cell treatment products, is relatively low in preparation cost and high in stem differentiation capability, and can remarkably improve the proliferation efficiency of the cultured mesenchymal stem cells.

The invention relates to the technical field of cell culture, and particularly provides a serum-free polypeptide culture medium for promoting proliferation of mesenchymal stem cells and a preparation method thereof, and the serum-free polypeptide culture medium for promoting proliferation of mesenchymal stem cells is prepared from dendrobium officinale, semen cuscutae, pseudo-ginseng, astragalus membranaceus, polygonum multiflorum, ginseng, artificial musk, oyster polypeptide, radix cyathulae polypeptide and the like. The serum-free polypeptide culture medium for promoting proliferation of the mesenchymal stem cells and the preparation method of the serum-free polypeptide culture medium are researched by further aiming at the culture environment of the adipose-derived mesenchymal stem cells and combining polypeptide and traditional Chinese medicine extracts.

The invention discloses a culture solution for inducing proliferation and differentiation of mesenchymal stem cells and a preparation method of the culture solution, the culture solution comprises the following raw materials in parts by weight: 40-60 parts of a basal culture medium, 5-10 parts of a serum substitute, 3-5 parts of growth factors, 1-3 parts of hormones, 1-3 parts of vitamins, 0.5-1 part of a small molecule compound and 1-3 parts of other additives, and the invention relates to the technical field of stem cell culture. According to the culture solution for inducing proliferation and differentiation of the mesenchymal stem cells and the preparation method of the culture solution, by optimizing basic components and adding specific growth factors and small molecule compounds, the proliferation capacity and directional differentiation efficiency of the MSCs are remarkably improved, and the culture solution is suitable for in-vitro induction of various tissues such as bones, cartilages and nerves. The culture solution adopts an animal-origin-free formula, has the advantages of low cost, high safety, strong applicability and the like, and provides an important technical tool for the field of regenerative medicine.

The invention discloses a method for large-scale acquisition of mesenchymal stem cell exosomes with high expression of IL-10 and application of the mesenchymal stem cell exosomes, and relates to the technical field of umbilical cord mesenchymal stem cell exosomes. The method comprises the following steps: carrying out cross culture on umbilical cord mesenchymal stem cells by adopting complete culture and hunger culture, and collecting a solution discharged from a cell factory when the hunger culture is finished each time, so as to obtain an exosome stock solution; and filtering the exosome stock solution to remove impurities, so as to obtain the mesenchymal stem cell exosome with high expression of IL-10. According to the method, proliferation of the umbilical cord mesenchymal stem cells can be effectively inhibited through alternate culture of the starvation culture medium and the complete culture medium, exosome secretion is stimulated, and high-yield, high-efficiency and stable exosome production is achieved. The mesenchymal stem cell exosome with high expression of IL-10 is applied to clinical treatment of HFpEF, has an anti-apoptosis protection effect on myocardial cells, and effectively promotes angiogenesis.

The invention discloses an induction culture medium for increasing the secretion amount of mesenchymal stem cells IL-10 and application of the induction culture medium, and belongs to the technical field of stem cell culture. In order to provide a method capable of improving the secretion amount of IL-10 of mesenchymal stem cells without influencing the proliferation of the mesenchymal stem cells, the method comprises the following steps: inducing amplified human mesenchymal cells by using a basic culture medium containing human IFN-gamma protein and TNF-alpha mimic peptide; and harvesting and culturing to obtain the human mesenchymal stem cells with increased IL-10 secretion amount. The induction culture medium provided by the invention can increase the secretion amount of mesenchymal stem cell IL-10 without influencing the proliferation of mesenchymal stem cells, and the mesenchymal stem cells obtained by induction are expected to be applied to the preparation of cell drugs for treating inflammatory diseases.

The invention discloses a preparation method of adipose-derived mesenchymal stem cells. The method comprises the following steps: (1) collecting adipose tissues; (2) carrying out enzymolysis on adipose tissues; (3) separating and purifying adipose tissues; and (4) proliferating the adipose-derived mesenchymal stem cells. According to the method disclosed by the invention, a mode of combining low-concentration enzymolysis and mechanical shearing is adopted, and meanwhile, a magnetic bead negative selection method is added, so that the cell separation efficiency is improved, the damage to cells is also remarkably reduced, and relatively high cell activity is maintained. Growth factors such as bFGF, insulin and HGF and small molecules are added in the cell proliferation culture process, the proliferation capacity of the cells is remarkably improved, and the pluripotency of the ADMSCs is guaranteed. The method provided by the invention can effectively promote the differentiation of ADMSCs to fat, bone and cartilage, and has stronger clinical application potential.

The application discloses a modified polyether ketone ketone composite material and a preparation method and application thereof, and relates to the technical field of composite materials, in particular to a modified polyether ketone ketone composite material, which comprises a sulfonated porous composite support, a chemotactic factor and a methacrylamide crystal glue; the preparation raw material of the sulfonated porous composite support comprises polyether ketone ketone and strontium-cerium doped bioactive glass; the chemotactic factor and the methacrylamide crystal glue are loaded in the pores of the sulfonated porous composite support. The three-dimensional porous composite support prepared by adopting PEKK and BG is used as the main body of the composite material, the chemotactic factor and the GelMA crystal glue are loaded in the pores, so that the immune regulation characteristics and the bone induction property of the PEKK-based support are enhanced; the prepared modified polyether ketone ketone composite material can significantly promote the proliferation and osteogenic differentiation of mesenchymal stem cells, is beneficial to the recruitment and polarization of macrophages, has a good bone formation effect in the immune-bone cascade reaction, and can accelerate the osteogenic differentiation process of the mesenchymal stem cells after being co-cultured with the macrophages.

The invention discloses a culture method for improving proliferation and migration potential of mesenchymal stem cells by using a traditional Chinese medicine composition, which adopts the traditional Chinese medicine composition taking red dates and cubilose as main components, and comprises 25 g of red dates, 25 g of cubilose, 10 g of tremella, 10 g of cranberries, 5 g of acerola cherries, 5 g of hawthorn, 5 g of astragalus membranaceus, 5 g of rehmannia, 5 g of donkey-hide gelatin and 5 g of II-type collagen cartilage powder. The preparation product is added with water, ground, filtered and then added into a mesenchymal stem cell culture medium to be cultured, and it is found that the water extract of the traditional Chinese medicine preparation can enhance the proliferation and migration potential of mesenchymal stem cells and increase the expression of anti-inflammatory factors in exosomes. The culture method of the mesenchymal stem cells is improved, so that the cell proliferation and migration potential of the mesenchymal stem cells is enhanced, the anti-inflammatory capacity of the mesenchymal stem cell exosome is enhanced, and the effect of the mesenchymal stem cells on treating the ovarian hypofunction is improved.

The invention discloses application of an extract in proliferation and differentiation of mesenchymal stem cells, and relates to the technical field of biological medicines. The invention specifically discloses perilla frutescens, black sesame, lophatherum gracile, lotus leaves, mulberry leaves, houttuynia cordata, angelica dahurica, smoked plum, fresh lalang grass rhizome, coix seeds, endive, fructus alpiniae oxyphyllae, astragalus membranaceus, dandelion fruits, chrysanthemum, dandelion, citron, gardenia, fresh rhizoma phragmitis, orange peel, semen raphani, mint, perilla seeds, fermented soybean, fructus cannabis, sealwort, agkistrodon halys, brassica juncea, red beans, red dates, hawthorn, bunge cherry seeds, raspberry, Chinese torreya, ginseng and the root of kudzu vine. The extract of at least one of semen ziziphi spinosae, exocarpium citri rubrum, black pepper, platycodon grandiflorum, emblic leafflower fruit, longan, hovenia dulcis thunb, agastache rugosus, Chinese olive, momordica grosvenori, semen cassiae, fingered citron, eucommia ulmoides, galangal, medlar and white hyacinth bean can regulate and control proliferation of the mesenchymal stem cells, and meanwhile, the multidirectional differentiation potential of the mesenchymal stem cells can be effectively maintained.

The invention discloses preparation and new application of growth hormone releasing hormone similar peptide monomers, dimers and tetramers, which can regulate the synergistic expression of human organ autocrine GHRH receptor down and GHSR up and down, directly promote the proliferation and / or activation of organ mesenchymal stem cells, and can be used for preparing medicines for treating organ injury, protecting diseases, and preventing and treating diseases. The compounds can be used as anti-aging, organ injury protection, cancer chemotherapy protection, anti-cancer drugs and the like.

The present invention belongs to the field of biomedicine and provides an application of epimedium vesicles in promoting the proliferation or osteogenic ability of mesenchymal stem cells. The present invention also provides a method for preparing mesenchymal stem cells loaded with epimedium vesicles: culturing mesenchymal stem cells until the confluence reaches 80%-95%; adding epimedium vesicles and co-culturing at 4°C and pH 6.8-7.4. The above-mentioned mesenchymal stem cells loaded with epimedium vesicles can be used to treat bone defects. The present invention solves the problem of maintaining the proliferation characteristics and osteogenic differentiation ability of UC-MSCs during long-term culture and passage.

The purpose of the present invention is to provide a culture medium for culturing having excellent proliferation of mesenchymal stem cells. The present invention is a mesenchymal stem cell proliferation promoter containing 16: 0 lysophosphatidic acid, a derivative of 16: 0 lysophosphatidic acid, and / or a salt of the 16: 0 lysophosphatidic acid and / or the derivative of 16: 0 lysophosphatidic acid. It is preferable that the mesenchymal stem cell proliferation promoter additionally contains 18: 1 lysophosphatidic acid, a derivative of 18: 1 lysophosphatidic acid, and / or a salt of the 18: 1 lysophosphatidic acid and / or the derivative of 18: 1 lysophosphatidic acid. The present invention also includes a culture medium for mesenchymal stem cells containing 16: 0 lysophosphatidic acid, a derivative of 16: 0 lysophosphatidic acid, and / or salts of the 16: 0 lysophosphatidic acid and the derivative of 16: 0 lysophosphatidic acid.

The invention belongs to the technical field of culture of mesenchymal stem cells, and particularly relates to a serum-free mesenchymal stem cell culture medium and a preparation method thereof. The culture medium comprises a basic culture medium and exogenous supplementary factors, wherein the exogenous supplementary factors comprise oligosaccharide peptide, highland barley fermentation liquor and multiflora rose glycoside; wherein the concentration of the oligosaccharide peptide in the basal culture medium is 10-50 [mu] g / mL, the concentration of the highland barley fermentation liquor in the basal culture medium is 30-80 [mu] g / mL, and the concentration of the multiflora rose glycoside in the basal culture medium is 1-5 [mu] g / mL. According to the culture medium disclosed by the invention, the oligosaccharide peptide, the highland barley fermentation liquor and the multiflora rose glycoside are added on the basis of the original basic culture medium, and the components are reasonably matched, so that the mesenchymal stem cells, particularly the proliferation and activity of the adipose-derived mesenchymal stem cells can be favorably maintained and stimulated; better proliferative activity and survival rate are shown.

The invention discloses a serum-free culture medium for hypoxia culture of mesenchymal stem cells as well as a preparation method and application of the serum-free culture medium. The serum-free culture medium comprises a basic culture medium, ginsenoside Rg1, asiaticoside, sodium pyruvate, recombinant human albumin and recombinant transferrin. The invention further discloses a preparation method of the corresponding serum-free culture medium. The preparation method comprises the steps of weighing and dissolving the components, filtering and sterilizing and the like. The invention also correspondingly discloses application of the serum-free culture medium. The sodium pyruvate, the recombinant human albumin and the recombinant transferrin are added to guarantee nutrition supply and function maintenance of the mesenchymal stem cells in a low-oxygen environment, and the ginsenoside Rg1 and the asiaticoside are added to synergistically promote proliferation, oxidation resistance and apoptosis resistance of the mesenchymal stem cells. The proliferation rate of the cultured mesenchymal stem cells and the expression and differentiation capacity of specific surface markers of the stem cells are improved, the apoptosis rate of the cells is remarkably reduced, and the method has wide application prospects in the fields of cell culture, regenerative medicine and the like.

The invention belongs to the technical field of cell culture, and particularly relates to application of isorhamnetin in preparation of a culture medium for promoting proliferation of mesenchymal stem cells. The concentration of isorhamnetin in the culture medium is 10-20 [mu] M. The culture medium for promoting proliferation of the mesenchymal stem cells, provided by the invention, is a growth culture medium added with a natural flavonoid compound isorhamnetin, proliferation of the adipose-derived mesenchymal stem cells can be remarkably promoted by adopting the improved culture medium for culture, and the requirement of cultured meat production on the number of seed cells is well met.

The invention discloses an aged mesenchymal stem cell as well as a preparation method and application thereof. The human mesenchymal stem cells containing eight amino acids are found to serve as an aging cell model and can show comprehensive aging characteristics, including increase of cell aging markers beta-galactosidase, p53, p21, p16 and gammaH2Ax, reduction of expression of Lamin B1 and pRb, increase of secretion of aging-related secretory phenotypes (SASP), accumulation of active oxygen, reduction of mitochondrial membrane potential and the like. Besides, the mesenchymal stem cells containing eight amino acids have higher proliferation activity than common mesenchymal stem cells under the same culture generations, and more iteratively cultured generations can be achieved, so that the culture cost of the mesenchymal stem cells serving as the senescence cell model is greatly reduced; the inventor puts forward that the mesenchymal stem cells containing eight amino acids can be used as a novel senescence cell model.

The invention belongs to the technical field of exosome application, discloses a preparation method and application of an exosome for promoting hair regeneration, and finds a traditional Chinese medicine composition which can be used for promoting proliferation of mesenchymal stem cells and promoting improvement of dryness of the mesenchymal stem cells. Besides, after the traditional Chinese medicine composition is used for intragastric administration of animals, serum (hereinafter referred to as drug-containing serum) containing the traditional Chinese medicine composition is generated, the process of taking traditional Chinese medicine by a human body is simulated, and after the generated drug-containing serum and the mesenchymal stem cells are co-incubated, the mesenchymal stem cell exosome obtained through separation can be used for preparing the mesenchymal stem cell exosome. Results show that the inductivity of the exosome to mesenchymal stem cells is enhanced, and compared with normal exosomes, the separated exosome has a better effect of promoting hair regeneration.

The application discloses application of Phascolosoma esculenta exosome in preparation of a preparation for promoting osteogenic differentiation of bone marrow mesenchymal stem cells, and belongs to the technical field of marine biological medical materials. Fresh and live Phascolosoma esculenta is used as raw material, body cavity fluid is collected, and Phascolosoma esculenta exosome is obtained through separation and purification by means of fractional filtration, differential centrifugation and ultracentrifugation. The exosome has uniform particle size, high biocompatibility and no cytotoxicity, can significantly promote proliferation of human bone marrow mesenchymal stem cells in a low concentration range of 5-10 μg / mL, up-regulate expression levels of ALP, RUNX2 and OPN osteogenic marker proteins, improve alkaline phosphatase activity, promote calcium nodule deposition, accelerate osteogenic differentiation of stem cells, and has good biological medicine development prospect and industrial application value.

The application belongs to the technical field of biology and particularly relates to a polypeptide for promoting mesenchymal stem cell proliferation and application thereof. The amino acid sequence of the polypeptide for promoting mesenchymal stem cell proliferation is shown as SEQ ID NO:1. The polypeptide for promoting umbilical cord mesenchymal stem cell proliferation is obtained by using a solid-phase synthesis method. The umbilical cord mesenchymal stem cell obtained by using the polypeptide for culture has good adhesion and proliferation capacity. In addition, the polypeptide can also promote Nrf2 gene expression, thereby effectively promoting the proliferation of umbilical cord mesenchymal stem cells.

The present invention relates to the technical field of stem cell detection, and particularly to a detection method based on human stem cells. The method includes preprocessing the acquired image data of the stem cell culture process to obtain analyzable image data; determining a prediction method for the number of stem cells based on the fluctuation degree of the proliferation rate of mesenchymal stem cells in a plurality of analyzable image data; determining to replace the culture medium or divide the mesenchymal stem cells into a new culture dish based on the proportion of the increased volume of mesenchymal stem cells and whether the mesenchymal stem cells show a multi-layer growth phenomenon under the condition that the deviation value is greater than a preset deviation value; determining to adjust the preset fluctuation degree or adjust the preset uniform distribution degree based on the occurrence frequency of the deviation value between the actual number and the predicted number of mesenchymal stem cells being greater than the preset deviation value under each prediction method after corresponding adjustment within a preset time period, and whether the image data corresponding to the occurrence time point has been preprocessed.