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Vitamin C-containing culture medium for promoting umbilical cord mesenchymal stem cell proliferation

A kind of stem cell and vitamin technology, applied in cell culture active agent, culture process, tissue culture and other directions, can solve the problems of cell aging and accelerated differentiation, and achieve the effect of promoting stem cell aging, reducing cell viability and promoting proliferation.

Active Publication Date: 2020-01-31
华辰未来(北京)生物医学技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, although cell passage can increase the number of cells, the aging and differentiation of cells are also accelerated with cell proliferation

Method used

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  • Vitamin C-containing culture medium for promoting umbilical cord mesenchymal stem cell proliferation
  • Vitamin C-containing culture medium for promoting umbilical cord mesenchymal stem cell proliferation
  • Vitamin C-containing culture medium for promoting umbilical cord mesenchymal stem cell proliferation

Examples

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with embodiment. However, the scope of the present invention is not limited to the following examples. Those skilled in the art can understand that various changes and modifications can be made in the present invention without departing from the spirit and scope of the present invention.

[0026] The present invention provides general and / or specific descriptions of the materials and test methods used in the tests. While many of the materials and methods of manipulation which are employed for the purposes of the invention are well known in the art, the invention has been described here in as much detail as possible.

[0027] The instruments, reagents, materials, etc. involved in the following examples, unless otherwise specified, are conventional instruments, reagents, materials, etc. in the prior art, and can be obtained through formal commercial channels. The experimental methods, detection methods...

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Abstract

The invention discloses application of vitamin C in promoting umbilical cord mesenchymal stem cell proliferation without influencing stem cell activity, viability, differentiation and senescence, andapplication of vitamin C in preparing a culture medium for promoting umbilical cord mesenchymal stem cell proliferation without influencing stem cell activity, viability, differentiation and senescence. A phenomenon is found that after a current stem cell culture medium is adopted to achieve stem cell adherence, AA is added (without other complex components being added), and then proliferation ofstem cells can be promoted. Through data, a result is proven that although AA can significantly increase the quantity of the stem cells within a short period of time, the activity, viability and cellcycle of the stem cells are not different from those of stem cells cultured in a control culture medium without AA. Therefore, a fact is illustrated that AA supplementation can simply promote cell proliferation, not reduce the activity and viability of the stem cells, and not promote stem cell senescence.

Description

technical field [0001] The invention relates to the application of vitamin C in promoting the proliferation of umbilical cord mesenchymal stem cells without affecting the activity, viability and differentiation aging of stem cells. and a culture medium containing vitamin C for promoting proliferation of umbilical cord mesenchymal stem cells, belonging to the technical field of cell culture. Background technique [0002] At present, stem cells, as a new treatment method, are being widely used in clinical trials of various diseases, and the vast majority of clinical trials have proved that stem cells can better improve symptoms. However, in the future clinical application of stem cells, it is necessary to solve how to proliferate to the number of cells that meet the use requirements in a shorter period of time, while ensuring that the mesenchymal stem cells still maintain good cell activity after large-scale expansion, and the number of proliferated cells remains high. Potent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0668C12N2500/38C12N2500/84C12N2501/06
Inventor 李一佳杜书文李玉静王苗孟繁敏郑伟涛
Owner 华辰未来(北京)生物医学技术有限公司
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