38results about How to "Activity will not decrease" patented technology

The invention discloses a compound microbial agent as well as a preparation method and application thereof. The compound microbial agent comprises bacillus, an embedding carrier and a cross-linking agent, wherein bacillus is immobilized in the embedding carrier via the cross-linking agent. The preparation method comprises the following steps: inoculating bacillus into a culture medium containing phenanthrene to undergo aerobic culture, thus obtaining a bacterial liquid; concentrating the bacterial liquid, adding the concentrated bacterial liquid to the embedding carrier to obtain a mixed liquid containing the bacterial liquid, and then dropwise adding the mixed liquid containing the bacterial liquid in the cross-linking agent to undergo cross-linking hardening to form a bacillus gel pellet; then carrying out aerobic culture, thus obtaining the compound microbial agent. The compound microbial agent can quickly and efficiently degrade high-concentration phenanthrene in wastewater under the condition that the wastewater contains high-concentration Pb<2+>. The preparation method has the advantages of simple process, low production cost, and the like.

The invention relates to a novel biological enzyme sensor capable of being implanted into a human body and a production method thereof. The surface of a substrate is cleaned via polishing, degreasing, activation and other physical and chemical processes, and the metal substrate is then coated with a layer of platinum black, platinum or gold via electroplating or sputtering. The surface of an electrode is modified with a layer of enzyme, the biological enzyme is cured through polymer embedding or cross-linking, one or more biocompatible polymer material(s) is/are used as a carrier, and the polymer material(s) as the carrier at least comprise(s) a polyamino, polyhydroxy or polythiol group. Reaction of polyisocyanate with polydimethylsiloxane and polyether polyol or polyester polyol, a small number of chain extenders are added, polyurethane prepolymer is produced, cross-linking curing of polyurethane prepolymer and polymer material(s) in the biological enzyme layer is carried out, the adhesion of the film can be improved, and the bio-sensing layer cannot fall off in long-time testing of an animal body or a human body. The method is simple, and the produced biological enzyme sensor has excellent mechanical flexibility, biocompatibility, adhesion and resistance to protein adsorption.

The invention discloses an application of a dehydroabietylamine-chiral thiourea catalyst in both-hands synthesizing gamma-nitro-aromatic ketones and gamma-nitro-aromatic mixed ketones chiral compounds in a high enantiomer. The invention successfully utilizes the chiral source of the nature to synthesize a noveldehydroabietylamine-chiral thiourea catalyst with high catalytic activity. The catalyst has low price, is easy to decorate and prepare, is simple in treatment, can be separated or recycled from the products, can be reutilized and extends the limited range of known thiourea catalyst. The invention realizes that the same catalyst in the same reaction system can generate high optical pure target product with different configurations by high-efficiency and high-activity asymmetric catalysis. The method has simple operation, uses no metal reagent and has less environmental pollution. The reaction condition is mild and the reaction can be carried out in air rather than the strict anhydrous and oxygen-free conditions.

The present invention discloses the synthesis process of double indolylalkyl derivative. Aldehyde and indole are made to produce synthesis reaction, and the aldehyde is selected from C1-C10 aliphatic aldehyde and aromatic aldehyde. The present invention features that super solid acid SO42-/TiO2 is used as catalyst in the amount of 30-80 mg for each millimole of aldehyde, and that double indolylalkyl compound is prepared through grinding reaction for 0.5-5 hr and subsequent washing with solvent, filtering and filtrate concentration. The present invention adopts super solid acid to replace traditional protonic acid or Lewis acid, and no solvent in preparing double indolylalkyl compound, and has no environmental pollution caused by organic solvent, high reaction yield, short production period and simple operation.

The invention relates to a method for making steamed bread by utilizing a multifunctional wheaten food cooking machine, comprising the following steps of: a, adding water into an inner barrel, driving a stirring knife, mixing and stirring materials to be uniform, so as to obtain a steamed bread base; b, stopping stirring, and fermenting the steamed bread base; and c, opening a steam rack cover, communicating holes of a steam rack with a lower steam chamber, and steaming the steamed bread by the way that steam produced by a steam generating device enters into an upper steam chamber and the lower steam chamber. Thus, appropriate flour water ratio can be effectively guaranteed and manual complex labour can be avoided, and dough water absorption consistency is ensured; meanwhile, heat of the steam can permeate into the the steamed bread base from the upper surface and the lower surface of the steamed bread base, steamed bread steaming speed is improved, steaming time is shortened, and danger of unsteamed bread can be avoided.

The present invention relates to the field of membrane proteomics research, specifically to a treatment method of a sample for membrane protein analysis identification. According to the treatment method of the present invention, SL is adopted as a detergent, and is provided for membrane protein analysis identification, wherein SL molecules have a linear hydrophobic hydrocarbon chain tail similar to SDS molecules, and a carboxyl head similar to SDC molecules, and experiment results show that SL provides significant advantages in identifications of membrane proteins, especially in identifications of integral membrane proteins having high hydrophobicity and/or multiple transmembrane regions compared with ALS and SDC.

The invention discloses novel application of tea polyphenol for promoting corn seed germination under heavy metal stress, a seed soaking liquid and a germination method. The seed soaking method comprises the following steps of: (1) sterilizing corn seeds; (2) repeatedly washing the sterilized corn seeds in distilled water; (3) soaking the corn seeds in clean water for 11-12 hours at 26-28 DEG C; (4) watering the corn seeds by using the seed soaking liquid, sealing by using a preservative film, and culturing with sunshine, wherein the seed soaking liquid comprises tea polyphenol; the content of tea polyphenol is 5-80mu g/mL. By virtue of tea polyphenol, the toxicity of chromium ions to seeds can be relieved, a certain promoting effect on corn seeds can be achieved, the activity of the corn seeds can be remarkably improved since seeds treated with tea polyphenol can be germinated in an adverse situation, the toxicity of chromium ions can be inhibited by oxidation of tea polyphenol, the content of chlorophyll can be effectively increased, the activity of CAT can be activated by antioxidation of tea polyphenol, plant cytomembrane peroxidation can be prevented, and the activity of CAT and POD treated with tea polyphenol can be improved.

The invention relates to a molecular modification method for improving the activity and stability of lysine decarboxylase. One end of a lysine decarboxylase (LDC) molecule is connected with a polypeptide segment (EK). The method includes the steps of firstly, obtaining a gene segment of the lysine decarboxylase molecule; secondly, connecting a gene segment for encoding an EK polypeptide to one endof a lysine decarboxylase gene; thirdly, connecting the lysine decarboxylase gene connected with an EK polypeptide gene to an expression carrier, transferring the gene into a host, and expressing themodified lysine decarboxylase (LDC-EK). By rationally modifying the enzyme molecule, the random mutation uncertainty and the complicated screening process are avoided; the modified lysine decarboxylase has high stability and catalytic activity. The catalytic activity of the LDC-EK is tripled, the high activity can be kept within the wide temperature range, and various organic solvents can be tolerated. The method is easier to operate, and protein is easier to prepare after modification.

The invention relates to a pharmaceutical composition containing PEGylated asparaginase. The composition comprises PEGylated asparaginase, sorbitol, a protective agent, a buffering agent and a surfactant; the protective agent is selected from mannitol, sucrose or lactose; and the buffering agent is selected from phosphate or Tris-HCl. The pharmaceutical composition containing PEGylated L-asparaginase is advantageous in that: after refrigeration, samples are not easy to degrade.

The invention discloses application of vitamin C in promoting umbilical cord mesenchymal stem cell proliferation without influencing stem cell activity, viability, differentiation and senescence, andapplication of vitamin C in preparing a culture medium for promoting umbilical cord mesenchymal stem cell proliferation without influencing stem cell activity, viability, differentiation and senescence. A phenomenon is found that after a current stem cell culture medium is adopted to achieve stem cell adherence, AA is added (without other complex components being added), and then proliferation ofstem cells can be promoted. Through data, a result is proven that although AA can significantly increase the quantity of the stem cells within a short period of time, the activity, viability and cellcycle of the stem cells are not different from those of stem cells cultured in a control culture medium without AA. Therefore, a fact is illustrated that AA supplementation can simply promote cell proliferation, not reduce the activity and viability of the stem cells, and not promote stem cell senescence.

The invention discloses a preparation method of a transfer factor. The preparation method of the transfer factor is characterized by comprising the steps: pre-processing, homogenation, repeated freeze thawing, centrifugation, ethanol precipitation, primary ultra-filtration, virus inactivation and secondary ultra-filtration. The invention also discloses a preparation method of a transfer factor injection. According to an ethanol precipitation method and a virus deactivation method which are not adopted in a traditional process, various impurity proteins are removed, and various animal source viruses are effectively removed, so that the security of products can be effectively guaranteed, sensitive sources are reduced, and the security is improved; meanwhile, by adopting an ethanol precipitation method, the impurity proteins are removed, so that the ultra-filtration load of a subsequent ultrafiltration membrane can be reduced, and the service life of the ultrafiltration membrane is prolonged.

The invention discloses a method for preparing a bimodal polyethylene adjustable in molecular weight distribution by use of a metallocene system. The method comprises the following steps: introducing the ethylene gas into a reaction container containing a dry organic solvent without water and oxygen under an electromagnetic stirring condition; after the ethylene gas is in a dissolution saturation state in the organic solvent, firstly adding a cocatalyst to the reaction container, and then adding a metallocene catalyst to start an ethylene polymerization reaction and adding a chain transfer agent to continue the polymerization reaction, and adding enough an acidified ethanol after the total polymerization reaction to terminate the reaction. The method is characterized in that the composition of the product and the molecular weight of the low molecular weight polyethylene can be regulated and controlled by adjusting the addition concentration and the addition time of an alkyl aluminum, and therefore, the bimodal polyethylene different in molecular weight distribution can be prepared; the addition of the alkyl aluminum is capable of improving the catalytic activity of the metallocene.

The invention discloses novel tea polyphenol induction salt-tolerant application, seed soaking solution and a salt-tolerant method for wheat seedlings. The wheat seedlings are irrigated with the seed soaking solution in salination environments when the wheat seedlings grow and the heights of the wheat seedlings reach 10 cm. The salt-tolerant method includes 1), disinfecting wheat seeds; 2), repeatedly thoroughly washing the disinfected wheat seeds in distilled water; 3), accelerating germination of the wheat seeds; 4), irrigating the wheat seedlings with the seed soaking solution for continuous three days when the wheat seedlings grow and the heights of the wheat seedlings reach 10 cm. The seed soaking solution contains tea polyphenol, and the content of the tea polyphenol is 10-40 micrograms/mL. The novel tea polyphenol induction salt-tolerant application, the seed soaking solution and the salt-tolerant method have the advantages that degradation of chlorophyll in leaves of the wheat seedlings can be obviously deferred under salt-stress conditions, the activity of CAT (catalase) and POD (peroxidase) can be improved, the proline content can be increased, accumulation of membrane lipid peroxidation products MDA can be reduced, and the salt-stress resistance of the wheat seedlings can be ultimately improved.

The invention discloses a method for efficiently producing extracellular vesicles. The method for producing the extracellular vesicles provided by the invention comprises the following steps: mixing cells with a biocompatible material to obtain a mixed solution; dispersing the mixed solution in a biological inert solution to form a biocompatible three-dimensional body; and culturing the biocompatible three-dimensional body in a cell culture medium, and collecting the supernatant to obtain the extracellular vesicles. According to the method, the cells are losslessly loaded to form a three-dimensional body, a good cell communication microenvironment is constructed, the efficiency of secreting extracellular vesicles by the cells is effectively improved, and the maximum utility utilization of the cells is achieved. The method is high in universality, simple in process, short in time consumption and high in uniformity, and can be used as a technical platform for large-scale production of the extracellular vesicles. Original forms, structures and functions of cells can be reserved, cell viability cannot be reduced, cell apoptosis is caused, or phenotypes of the cells cannot be changed, and therefore functions of diagnosis, treatment and the like of extracellular vesicles cannot be weakened.

The invention discloses a method for preparing cyclohexanone from cyclohexanol. The method comprises the following steps: preparing a copper-based catalyst adopting alumina and graphene as a carrier,mixing cyclohexanol, hydrogen peroxide and water to prepare reaction raw materials, contacting the reaction raw materials with the catalyst, and carrying out a reaction at 30-110 DEG C under 0.1-1.5 MPa to prepare the cyclohexanone. The prepared catalyst has the advantages of effective improvement of the selectivity and the yield of the reaction, increase of the cyclohexanol utilization rate, small use amount and low preparation cost.

The invention relates to microsphere production of the field of pharmacy, and discloses pancreatin microspheres and a production method thereof. The microspheres are prepared from pancreatin, a medicinal auxiliary and one or more hydrophilic low-melting-point polymer, wherein the weight percentage of the pancreatin is 40%-85%, the weight percentage of the hydrophilic low-melting-point polymer is 15%-50%, and the rest is the medicinal auxiliary. The production method of the pancreatin microspheres comprises the step that without solvents, through high-energy granulation and a spheroidization technology, the pancreatin microspheres are produced; and no solvents are used in the production process, the prepared microspheres have high enzymatic activity, bioavailability and stability, and compared with existing production methods, the production method is more effective.

The invention discloses traditional Chinese medicine decoction for treating arthritis and a preparation method for the traditional Chinese medicine decoction. The traditional Chinese medicine decoction is prepared from the following raw materials in parts by weight: 30 to 35 parts of munronia henryi harms, 12 to 15 parts of fmoes officinalis ames, 20 to 25 parts of croton seed, 10 to 15 parts of rosamultiflora, 22 to 25 parts of semiliquidambar cathayensis, 20 to 25 parts of conespike flemingia root, 15 to 20 parts of wilson's passion flower, 10 to 15 parts of puberulent premna leaf or root, 8 to 10 parts of chinaroot greenbrier, 2 to 5 parts of kirilow groundsel herb, 2 to 3 parts of enemion raddeanum, 15 to 18 parts of buddleja asiatica, 8 to 12 parts of stachys sieboldii miq., 1 to 2 parts of veined inula root, 4 to 8 parts of litsea rotundifolia and 3 to 5 parts of luisia morsei. The traditional Chinese medicine decoction for treating arthritis has a good treatment effect and short treatment time, is capable of reducing pain of a patient, and has no side effect.