A dual-mode output optical tweezers based on hollow beam

Abstract

Provided in the invention is dual-mode output optical tweezers based on a hollow beam. The dual-mode output optical tweezers comprise a laser device, a coupling lens, a multi-mode fiber, a focusing lens, a laser cavity, a beam-splitting lens and a reflecting lens that are arranged successively. Laser emitted by the laser device is focused by the coupling lens and then the focused laser enters themulti-mode fiber in a manner of deviating from the central axis of the multi-mode fiber, wherein the distance between the incident position of the laser and the central axis of the multi-mode fiber is15 microns to 50 microns; the light beam emitted by the multi-mode fiber passes through the focusing lens and then enters a crystal in the laser cavity; two light beams that have different wavelengths and are generated by excitation of the crystal are outputted from the laser cavity and then the outputted light beams are split into two paths by the beam-splitting lens: one path of light beam is capturing light that passes through the beam-splitting lens and then irradiates a to-be-measured object in a sample region and the other path of light beam is detection light that is reflected by the beam-splitting lens and the reflecting lens successively and then irradiates a to-be-measured object in the sample region. According to the invention, the optical tweezers can output hollow beams in adual-mode manner as capturing light and detection light and the hollow beams do not affect the activity of the microscopic particle. Moreover, the dual-mode output optical tweezers have a simplified structure.

Description

technical field [0001] The invention belongs to the technical field of applied optics, in particular to an optical tweezers capable of outputting hollow beams in dual modes. Background technique [0002] Optical tweezers is a physical tool based on the mechanical effect of laser, which can use the radiation pressure of light to realize the operation of tiny particles. Most of the light beams generated by existing optical tweezers are solid beams, so microscopic particles will be trapped in the central area with strong light intensity. For active microscopic particles, since the central laser light intensity of the solid beam is strong, the activity of the particles will be reduced, and even the particles will be killed; and when detecting, the strong light of the solid beam will also interfere with the detection of fluorescence, which will affect the imaging band. to greater interference. Moreover, the existing optical tweezers systems generally use two sets of light sourc...

AI Technical Summary

Problems solved by technology

For active microscopic particles, since the central laser light intensity of the solid beam is strong, the activity of the particles will be reduced, and even the particles will be killed; and when detecting, the strong light of the solid beam will also interfere with the detection of fluorescence, which will affect the imaging band. more interference

Moreover, the existing optical tweezers system generally uses two sets of light sources to generate the capture light and the probe light respectively, and the optical path structure is complex.

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