Novel application of tea polyphenol for promoting corn seed germination under heavy metal stress, seed soaking liquid and germination method
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A technology of seed soaking solution and tea polyphenols, which is applied in the fields of seed and rhizome treatment, botanical equipment and methods, and application, etc., can solve the problems of poor mental development of the next generation.
Active Publication Date: 2016-09-28
CHANGSHU INSTITUTE OF TECHNOLOGY
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Parents who have been exposed to hexavalent chromium for a long time may also have adverse effects on the intellectual development of their offspring
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Embodiment 1
[0023] (1) putting corn seeds into a concentration of 1% potassium permanganate solution and soaking for half an hour, and stirring constantly, so as to sterilize the seeds;
[0024] (2) repeatedly rinsing the sterilized corn seeds in distilled water;
[0025] (3) Put the corn seeds into clear water and soak the seeds at 26-28°C for 12 hours;
[0026] (4) Water the corn seeds with the soaking solution, then seal them with plastic wrap, and cultivate them under the sun; the soaking solution contains 40 mg / L of K 2 Cr 2 o 7 .
Embodiment 2
[0028] (1) putting corn seeds into a concentration of 1% potassium permanganate solution and soaking for half an hour, and stirring constantly, so as to sterilize the seeds;
[0029] (2) repeatedly rinsing the sterilized corn seeds in distilled water;
[0030] (3) Put the corn seeds into clear water and soak the seeds at 26-28°C for 11 hours;
[0031] (4) Irrigate the corn seeds with the soaking solution, then seal them with plastic wrap, and cultivate them in the sun; the soaking solution contains tea polyphenols with a content of 5 μg / mL and K of 40 mg / L 2 Cr 2 o 7 .
Embodiment 3
[0033] (1) putting corn seeds into a concentration of 1% potassium permanganate solution and soaking for half an hour, and stirring constantly, so as to sterilize the seeds;
[0034] (2) repeatedly rinsing the sterilized corn seeds in distilled water;
[0035] (3) Put the corn seeds into clear water and soak the seeds at 26-28°C for 12 hours;
[0036] (4) Water the corn seeds with the soaking solution, then seal them with plastic wrap, and cultivate them in the sun; the soaking solution contains tea polyphenols with a content of 20 μg / mL and K of 40 mg / L. 2 Cr 2 o 7 .
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Abstract
The invention discloses novel application of tea polyphenol for promoting corn seed germination under heavy metal stress, a seed soaking liquid and a germination method. The seed soaking method comprises the following steps of: (1) sterilizing corn seeds; (2) repeatedly washing the sterilized corn seeds in distilled water; (3) soaking the corn seeds in clean water for 11-12 hours at 26-28 DEG C; (4) watering the corn seeds by using the seed soaking liquid, sealing by using a preservative film, and culturing with sunshine, wherein the seed soaking liquid comprises tea polyphenol; the content of tea polyphenol is 5-80mu g/mL. By virtue of tea polyphenol, the toxicity of chromium ions to seeds can be relieved, a certain promoting effect on corn seeds can be achieved, the activity of the corn seeds can be remarkably improved since seeds treated with tea polyphenol can be germinated in an adverse situation, the toxicity of chromium ions can be inhibited by oxidation of tea polyphenol, the content of chlorophyll can be effectively increased, the activity of CAT can be activated by antioxidation of tea polyphenol, plant cytomembrane peroxidation can be prevented, and the activity of CAT and POD treated with tea polyphenol can be improved.
Description
technical field [0001] The invention relates to a new application of tea polyphenols for promoting germination of corn seeds under heavy metal stress. The invention also relates to a seed soaking liquid containing tea polyphenols, and a germination method using the above seed soaking liquid. Background technique [0002] Chromium is an element widely found in nature. Mainly distributed in rocks, soil, air, water and organisms. Chromium is widely distributed in soil. The content range is very wide, and the chromium content in water and the atmosphere is relatively small. Animals and plants contain trace amounts of chromium. Chromium exists mainly in the form of divalent chromium and hexavalent chromium in nature. Divalent chromium participates in the metabolism of sugar and fat in humans and animals, and is an essential trace element for the human body; hexavalent chromium is a clear harmful element that can precipitate certain proteins in human blood, causing anemia, ne...
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