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A bioenzyme sensor implantable in human body and its manufacturing method

A production method and bio-enzyme technology, applied in sensors, medical science, diagnostic recording/measurement, etc., can solve the problems of outer membrane layer adhesion, biocompatibility and protein resistance, and achieve good protein adsorption resistance , excellent mechanical elasticity, easy preparation and operation

Active Publication Date: 2015-08-19
SINOCARE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Although these literatures have made some explorations on the outer membrane layer, the research on the adhesion, biocompatibility, and protein resistance of the outer membrane layer is not enough, especially in the specific application of the glucose sensor working electrode.

Method used

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  • A bioenzyme sensor implantable in human body and its manufacturing method
  • A bioenzyme sensor implantable in human body and its manufacturing method
  • A bioenzyme sensor implantable in human body and its manufacturing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1. Polish the stainless steel needle with 300-grit sandpaper, and decorate a layer of platinum black on the substrate after degreasing, activation and electroplating tools; 2. Use polyvinyl alcohol as a carrier to embed glucose oxidase and polythioalkyd resin In the electrode, dry at room temperature; 3, the excess (n NCO :n NH2 / OH / SH The value is controlled at 1.3) of 4,4'-dicyclohexylmethane diisocyanate (HMDI) reacts with polyester diol and polysiloxane, and adds a small amount of dibutylamine to generate polyurethane prepolymer, and the reaction time is After 10 hours, the reaction temperature was 45°C. After the reaction, it was purified by liquid phase preparative chromatography. The prepolymer was modified on the enzyme layer by dip coating, and then cured by heat to form a film. The prepared biosensing layer had good adhesion. It does not fall off in animal or human long-term tests, and has good biocompatibility and anti-protein adsorption performance.

[0045...

Embodiment 2

[0047] 1. Polish the titanium wire with 400-mesh sandpaper, and decorate a layer of gold on the substrate after degreasing, activation and electroplating tools; 2. Using hydroxycellulose as a carrier, embed glucose oxidase and agarose in the electrode, Dry at room temperature; 3, the excess (n NCO :n NH2 / OH / SH The value is controlled at 1.4) isophorone diisocyanate (IPDI) reacts with polyether diol and polysiloxane, and adds a small amount of 1,4-butanediol to generate polyurethane prepolymer, and the reaction time is 8 Hours, the reaction temperature is 55 ° C, after the reaction, it is purified by liquid phase preparative chromatography. The prepolymer is modified on the enzyme layer by spraying, and then cured by ultraviolet light to form a film. The prepared biosensing layer has good adhesion. It does not fall off in animal or human long-term tests, and has good biocompatibility and anti-protein adsorption performance.

[0048] According to the bioenzyme sensor that can ...

Embodiment 3

[0050] 1. Polish the aluminum wire with 300-mesh sandpaper, and decorate a layer of platinum black on the substrate after degreasing, activation and electroplating tools; 2. Using polyacrylamide as a carrier, embed glucose oxidase and heparin in the electrode, Dry in the oven; 3, the excess (n NCO :n NH2 / OH / SH The value is controlled at 1.5) of isophorone diisocyanate (IPDI) reacts with polyether diol and polysiloxane, and adds a small amount of 1,4-butanediol to generate polyurethane prepolymer, and the reaction time is 6 hours, the reaction temperature is 65°C, and after the reaction, it is purified by liquid phase preparative chromatography. The prepolymer is modified on the enzyme layer by brushing, and then cured by ultraviolet light to form a film. The prepared biosensing layer has good adhesion. It does not fall off in animal or human long-term tests, and has good biocompatibility and anti-protein adsorption performance.

[0051] According to the bioenzyme sensor that...

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Abstract

The invention relates to a novel biological enzyme sensor capable of being implanted into a human body and a production method thereof. The surface of a substrate is cleaned via polishing, degreasing, activation and other physical and chemical processes, and the metal substrate is then coated with a layer of platinum black, platinum or gold via electroplating or sputtering. The surface of an electrode is modified with a layer of enzyme, the biological enzyme is cured through polymer embedding or cross-linking, one or more biocompatible polymer material(s) is / are used as a carrier, and the polymer material(s) as the carrier at least comprise(s) a polyamino, polyhydroxy or polythiol group. Reaction of polyisocyanate with polydimethylsiloxane and polyether polyol or polyester polyol, a small number of chain extenders are added, polyurethane prepolymer is produced, cross-linking curing of polyurethane prepolymer and polymer material(s) in the biological enzyme layer is carried out, the adhesion of the film can be improved, and the bio-sensing layer cannot fall off in long-time testing of an animal body or a human body. The method is simple, and the produced biological enzyme sensor has excellent mechanical flexibility, biocompatibility, adhesion and resistance to protein adsorption.

Description

technical field [0001] The present invention relates to a new bioenzyme sensor that can be implanted into the human body and its manufacturing method. The sensor that can be implanted into the human body is suitable for continuous monitoring of various physiological indicators and drug metabolism of the human body, especially for the monitoring of dynamic blood sugar in the human body. . Background technique [0002] Biosensor is an analysis system that uses electrochemical principles to use biologically active substances such as enzymes, antibodies, antigens, microorganisms, cells, tissues, and nucleic acids as sensitive elements, and converts their concentrations into electrical signals to quickly, accurately, and conveniently measure the content of biological substances. , the constituent materials must have specific properties in order to function in vivo and provide appropriate signals. [0003] The bioenzyme sensor implanted in the human body is generally composed of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61B5/1486
Inventor 周建华车宏莉
Owner SINOCARE
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