New application of tea polyphenols inducing salt resistance of wheat seedlings, seed soaking solution, and salt resistance methods
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A technology of seed soaking liquid and tea polyphenols, which is applied in the field of inducing salt resistance of wheat seedlings, can solve the problem of few applications
Active Publication Date: 2018-10-09
苏州智品尚生物科技有限公司
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Problems solved by technology
The research of tea polyphenols on animals has become a hot spot in the scientific community, but its application on plants is seldom
Method used
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Embodiment 1
[0024] (1) Select plump, uniform seeds and sterilize them with 0.1% potassium permanganate for 20 minutes,
[0025] (2) repeatedly rinse the sterilized wheat seeds in distilled water;
[0026] (3) Wheat seeds are placed in a petri dish at a temperature of 25° C. under natural light to germinate the wheat seeds;
[0027] (4) When the wheat seedlings grew to a height of 10 cm, the wheat seedlings were watered with distilled water for three consecutive days.
Embodiment 2
[0029] (1) Select plump, uniform seeds and sterilize them with 0.1% potassium permanganate for 20 minutes,
[0030] (2) repeatedly rinse the sterilized wheat seeds in distilled water;
[0031] (3) Wheat seeds are placed in a petri dish at a temperature of 25° C. under natural light to germinate the wheat seeds;
[0032] (4) When the wheat seedlings grew to a height of 10 cm, the wheat seedlings were watered for three consecutive days with the soaking solution; the soaking solution contained 200 mmol / L NaCl.
Embodiment 3
[0034] (1) Select plump, uniform seeds and sterilize them with 0.1% potassium permanganate for 20 minutes,
[0035] (2) repeatedly rinse the sterilized wheat seeds in distilled water;
[0036] (3) Wheat seeds are placed in a petri dish at a temperature of 25° C. under natural light to germinate the wheat seeds;
[0037] (4) When the wheat seedlings grow to a height of 10cm, water the wheat seedlings with the soaking solution for three consecutive days; the soaking solution contains 200mmol / L NaCl, based on NaCl, the soaking solution contains 10 μg / ml tea polyphenols .
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Abstract
The invention discloses novel tea polyphenol induction salt-tolerant application, seed soaking solution and a salt-tolerant method for wheat seedlings. The wheat seedlings are irrigated with the seed soaking solution in salination environments when the wheat seedlings grow and the heights of the wheat seedlings reach 10 cm. The salt-tolerant method includes 1), disinfecting wheat seeds; 2), repeatedly thoroughly washing the disinfected wheat seeds in distilled water; 3), accelerating germination of the wheat seeds; 4), irrigating the wheat seedlings with the seed soaking solution for continuous three days when the wheat seedlings grow and the heights of the wheat seedlings reach 10 cm. The seed soaking solution contains tea polyphenol, and the content of the tea polyphenol is 10-40 micrograms / mL. The novel tea polyphenol induction salt-tolerant application, the seed soaking solution and the salt-tolerant method have the advantages that degradation of chlorophyll in leaves of the wheat seedlings can be obviously deferred under salt-stress conditions, the activity of CAT (catalase) and POD (peroxidase) can be improved, the proline content can be increased, accumulation of membrane lipid peroxidation products MDA can be reduced, and the salt-stress resistance of the wheat seedlings can be ultimately improved.
Description
technical field [0001] The invention relates to a new application of tea polyphenols inducing salt resistance of wheat seedlings. The invention also relates to a seed soaking solution containing tea polyphenols and a method for inducing salt resistance of wheat seedlings by using the above seed soaking solution. Background technique [0002] Tea polyphenols, also known as tea tannins and tea tannins, are a general term for a class of polyhydric phenolic compounds contained in tea, abbreviated as TP. Its main components include catechins, flavonoids, flavonols, anthocyanins, phenolic acids and depsipated acids, of which catechins account for about 65%-80% of the total tea polyphenols, including four Forms of catechins: Epigallocatechin gallate (EGCG), Epigallocatechin (EGC), Epigallocatechin gallate (ECG), Epigallocatechin (EC), Catechins The acidic phenolic hydroxyl groups on the B ring and C ring of the compound have strong hydrogen donating ability, which can interrupt t...
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Application Information
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