The invention provides a cerebrolysin
hydrolysate injection and a preparation method thereof. The preparation method comprises the following steps: taking
pig brain, removing impurities, and adding
purified water for homogenizing; hydrolyzing homogenate through
pepsin and pancreatin, and collecting a
clear liquid; regulating the pH of the
clear liquid to be 1.7-3, heating to 100-110 DEG C, insulating for 15-45min, then regulating pH to 8.0-9.0; conducting
ultrafiltration through a 8-10kd membrane, and collecting filtrate; conducting
column chromatography to the filtrate, collecting an eluent, regulating ph to 8.0-9.0, concentrating through an anion-exchange membrane, filtering through a 0.2mu m membrane to obtain a stock liquid; and freezing the stock liquid, then defreezing, adding an
antioxidant, regulating the pH to be 6.9-7.5, and filtering respectively through a 8-10kd membrane and a 0.2mu m membrane. The polypeptide in the obtained cerebrolysin
hydrolysate injection is similar to that of a control
drug being more than or equal to 0.90, and the obtained cerebrolysin
hydrolysate injection contains 16 amino acids, the
nitrogen amount of
total amino acids accounts for more than or equal to 85% of
total nitrogen content, additional amino acids are not required, and the amino acids can be prepared completely by means of
pig brain, thus being stable and safe.