Preparation method of rape bee pollen long chain fatty acids and derivatives thereof

Abstract

The present invention discloses a preparation method of rape bee pollen long chain fatty acids and derivatives thereof. The preparation method comprises the following steps: a) providing an extract of rape bee pollen after washing and centrifuging; b) using microwave to vacuum dry the extract; c) conducting supercritical fluid extraction to obtain a supercritical extract; d) extracting the supercritical extract with C1-C5 alcohols or aqueous solutions thereof to get rape bee pollen long chain fatty acids and the derivatives thereof. The preparation method of rape bee pollen long chain fatty acids and derivatives thereof is high in controllability, low in energy consumption, low in pollution and low in cost, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and in particular relates to a preparation method of rapeseed bee pollen long-chain fatty acid and derivatives thereof. Background technique [0002] Clinical practice at home and abroad has proved that pollen and its preparations are ideal drugs for the treatment of prostate diseases. Li Yonghui (Li Yonghui. Research on the pharmacodynamic substance basis of vegetable pollen against benign prostatic hyperplasia. Master's degree thesis of Shanghai Institute of Pharmaceutical Industry (D), 2009) reported in the literature that rape bee pollen, vegetable pollen, corn pollen, Pine pollen and sunflower pollen. The research results show that rape bee pollen has a strong anti-prostatic hyperplasia effect, and the best effective components of rape bee pollen anti-prostatic hyperplasia are fatty acids and flavonoids. In the process of drug efficacy screening research, it was found t...

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Problems solved by technology

[0004] According to the disclosure of patent CN 101933947 A, the paste after washing and centrifuging rapeseed bee pollen needs to be dried to a water content ≤ 10% by mass before supercritical extraction can be performed. The vacuum drying and spray drying methods disclosed in this application have been found to be low-dose after testing. The sample can be dried quickly, but after the test amount increases to the kilogram level, the vacuum-dried sample is fluid, and the test time is extended by more than 3 times. After drying, the sample is light yellow or black, and the color is uneven; the spray-dried sample is unevenly dried. After the pollen humidity is as high as 10%, repeated operations are required, and the drying time is greatly extended

Vacuum drying and spray drying are used to dry the pollen water washing extract. The experimental design is not careful, which increases the experimental error, and the operation is not controllable, so it is not suitable for industrial production.

[0005] On the other hand, the main components of the supercritical extract of rapeseed bee pollen are long-chain fatty acids and their derivatives, but also contain a large number of compounds with invalid components, which are not suitable for direct development as new drugs. According to the purification of long-chain fatty acids disclosed in patent CN 101347490 A The compounds obtained by the method of its derivatives and its derivatives have been proved by pharmacodynamic experiments to have a good curative effect on prostate diseases, which mainly include linolamide, glyceryl linolenate, ethyl linolenate, lauric acid, linolenic acid, 10-form Oxy-1-heptadecen-4,6-diynyl-3,9-diol, flounder acid, eicosadioic acid, 12-methyl-myristate methyl ester, linolenic acid, oil Acid, anacardic acid A, stigmasterol ferulate and other long-chain fatty acids and their derivatives, but because of the characteristics of long-chain fatty acids and their derivatives, the supercritical extract of rape bee pollen is extremely difficult to hang on the column, and it is effective After the components are eluted, because the less polar non-active components still remain on the column, they are not easy to elute, and the next batch of production cannot be directly carried out, so the enrichment of active components with macroporous resins is not suitable for industrial production

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