Culture solution, application of culture solution and method for culturing umbilical cord mesenchymal stem cells

Abstract

The invention relates to the technical field of culture of stem cells, in particular to a culture solution, an application of the culture solution and a method for culturing umbilical cord mesenchymal stem cells. The culture solution provided by the invention consists of a serum-free medium, albumin, stem cell factors and epidermal growth factors. The umbilical cord mesenchymal stem cells, which are cultured with the application of the culture solution provided by the invention, are high in quantity and living cell rate and are good in stem cell maintenance. Experiments prove that the umbilical cord mesenchymal stem cells cultured by virtue of the culture medium provided by the invention can reach a density to 1.2*10<7>cells / mL or above and a living cell rate to 95% or above; and by conducting surface marker flow cytometry, the umbilical cord mesenchymal stem cells completely conform to properties of umbilical cord mesenchymal stem cells.

Description

technical field [0001] The invention relates to the technical field of stem cell culture, in particular to a culture solution and its application and a method for culturing umbilical cord mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells are derived from the mesoderm and ectoderm in the early stages of development, and have the characteristics of self-replication, self-renewal, multi-directional differentiation potential, hematopoietic support, and immune regulation. At present, mesenchymal stem cells are mainly extracted from fat, bone marrow, umbilical cord, and placenta tissue. Mesenchymal stem cells still have multi-directional differentiation potential after continuous subculture and cryopreservation, which provides a new way for the clinical treatment of various diseases, such as nervous system diseases, kidney diseases, autoimmune diseases, and malignant tumors. In addition, mesenchymal stem cells can secrete a variety of stem cell factors ...

AI Technical Summary

Problems solved by technology

However, there are many unknown components in the serum, and may even include some unknown allergens and pathogens. On the one hand, it will interfere with the experimental results. On the other hand, the safety of the cultured cells cannot be guaranteed, and it cannot be used in clinical research.

However, the serum-free medium currently cultivated is not effective for the cultivation of umbilical cord mesenchymal stem cells, and the cells grow slowly and have low viability.