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Mahonia is a genus of approximately 70 species of evergreen shrubs and rarely small trees in the family Berberidaceae, native to eastern Asia, the Himalaya, North and Central America. They are closely related to the genus Berberis and botanists disagree on whether to recognize a separate Mahonia. Some authorities argue Mahonia should be included in Berberis because several species in both genera are able to hybridize, and because when the two genera are looked at as a whole, no consistent morphological separation exists except simple versus compound leaves. However, recent DNA-based phylogenetic studies support recognition of Mahonia, though after the removal of several species into the newly-described genera Alloberberis (formerly Mahonia section Horridae) and Moranothamnus (formerly Mahonia claireae). Mahonia species typically have large, pinnate leaves 10–50 cm (3.9–19.7 in) long with five to 15 leaflets, and flowers in racemes which are 5–20 cm (2.0–7.9 in) long.
Fingerprint detection method of Mahonia bealei and its identification and extraction method
The present application belongs to the technical field of traditional Chinese medicine modernization, and particularly relates to a fingerprint spectrum detection method of Mahonia leaf, and an identification and extraction method thereof. The fingerprint spectrum detection method of Mahonia leaf comprises the following steps: (1) preparation of a reference solution; (2) preparation of a sample solution: taking a Mahonia leaf sample and adding an extraction solvent B for extraction; (3) performing ultra-high performance liquid chromatography analysis on the reference solution and the sample solution; and (4) establishing a fingerprint spectrum of the Mahonia leaf medicinal material. The fingerprint spectrum detection method of Mahonia leaf can identify Mahonia leaf of different origins, and screen common characteristic peaks and / or potential differential components of Mahonia leaf of different origins. In addition, the extraction method can extract index components of Mahonia leaf through column layer analysis. The chemical fingerprint spectrum method of the present application is stable and reliable, can significantly distinguish Mahonia leaf of different origins, and can provide a reference for quality control, evaluation and origin identification of Mahonia leaf, and has important significance for ensuring clinical efficacy.
Owner:TONGJITANG CHINESE MEDICINES CO