Human mesenchymal stem cell proliferation promotion adjuvant, human mesenchymal stem cell amplification, pharmaceutical composition, growth factor and use

A technology of mesenchymal stem cells and growth factors, applied in the field of rapid proliferation of human mesenchymal stem cells in vitro, can solve the problems of slow proliferation and pollution of somatic cells, and achieve the effects of reducing cell aging, avoiding cross-contamination of pathogenic sources, and prolonging time

Active Publication Date: 2015-03-25
BUDDHIST TZU CHI GEN HOSPITAL
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Problems solved by technology

However, the culture method revealed by it is to put somatic cells into a basal medium DMEM (Dulbecco`s Modified Eagle Medium) containing 10% fetal bovine serum (10% FBS) for culture. The secreted growth factors are relatively s

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  • Human mesenchymal stem cell proliferation promotion adjuvant, human mesenchymal stem cell amplification, pharmaceutical composition, growth factor and use
  • Human mesenchymal stem cell proliferation promotion adjuvant, human mesenchymal stem cell amplification, pharmaceutical composition, growth factor and use
  • Human mesenchymal stem cell proliferation promotion adjuvant, human mesenchymal stem cell amplification, pharmaceutical composition, growth factor and use

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[0069]In order to make the above-mentioned and other objects, features, and advantages of the present invention more obvious and understandable, the preferred embodiments are specifically cited below, together with the accompanying drawings, and are described in detail as follows:

[0070] The present invention provides an adjuvant for the rapid proliferation of human mesenchymal stem cells in vitro, which includes at least one antioxidant and a second type of fibroblast growth factor (FGF-2); wherein the aforementioned antioxidant includes a long-acting ascorbic acid derivative and a combination of N-acetyl-L-cysteine ​​(NAC). Preferably, the long-acting ascorbic acid derivative is L-ascorbic acid-2-phosphate (Asc 2-P), and the concentration of type II fibroblast growth factor is 1 to 20 ng / ml.

[0071] Preferably, the aforementioned human mesenchymal stem cells are selected from one of the groups consisting of adipose-derived mesenchymal stem cells, bone marrow mesenchymal s...

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Abstract

The invention relates to a human mesenchymal stem cell in-vitro rapid proliferation adjuvant used to solve the problem of poor cell amplification efficiency of human mesenchymal stem cell culture. The human mesenchymal stem cell culture adjuvant comprises at least an antioxidant and a second type fibroblast growth factor (FGF-2). Thereby, after the adjuvant is added into a medium containing human mesenchymal stem cells for culture in normoxic environment (with the oxygen partial pressure being about 21%), phenomena such as rapid cell division, increase of cell cycle period synthesis phase proportion, reduction of aging, improvement of the differentiation potential, and the like, the human mesenchymal stem cell in-vitro rapid proliferation adjuvant can not only rapidly expand the human mesenchymal stem cells and obtain the growth factor, can still keep the more functional characteristic of the stem cells, and can be used for the purposes of human mesenchymal stem cell culture and amplification.

Description

technical field [0001] The present invention relates to an adjuvant for the rapid proliferation of human mesenchymal stem cells in vitro, especially an adjuvant containing antioxidants and growth factors to be added to the culture medium of human mesenchymal stem cells to make human mesenchymal stem cells grow in the first generation Or subculture can rapidly proliferate and obtain growth factors. Background technique [0002] Press, the current scientific community has given some basic definitions and characteristics of stem cells: it refers to a group of cells that have the ability of self-renewal and proliferation, and can maintain the state of undifferentiation (Undifferentiation) for a long time, and The multi-differentiation characteristics of cell groups that can differentiate into different lineages and tissues with specific functions after being properly induced and stimulated. [0003] Depending on the source of stem cells and their differentiation potential, they...

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Application Information

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IPC IPC(8): C12N5/0775C12P21/00A61K35/28A61K38/18A61K8/64A61P17/02A61Q19/00A61Q19/08C40B40/02
Inventor 孙立易冯清荣郑敬枫李典锟
Owner BUDDHIST TZU CHI GEN HOSPITAL
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