Human mesenchymal stem cell proliferation promotion adjuvant, human mesenchymal stem cell amplification, pharmaceutical composition, growth factor and use

Abstract

The invention relates to a human mesenchymal stem cell in-vitro rapid proliferation adjuvant used to solve the problem of poor cell amplification efficiency of human mesenchymal stem cell culture. The human mesenchymal stem cell culture adjuvant comprises at least an antioxidant and a second type fibroblast growth factor (FGF-2). Thereby, after the adjuvant is added into a medium containing human mesenchymal stem cells for culture in normoxic environment (with the oxygen partial pressure being about 21%), phenomena such as rapid cell division, increase of cell cycle period synthesis phase proportion, reduction of aging, improvement of the differentiation potential, and the like, the human mesenchymal stem cell in-vitro rapid proliferation adjuvant can not only rapidly expand the human mesenchymal stem cells and obtain the growth factor, can still keep the more functional characteristic of the stem cells, and can be used for the purposes of human mesenchymal stem cell culture and amplification.

Description

technical field [0001] The present invention relates to an adjuvant for the rapid proliferation of human mesenchymal stem cells in vitro, especially an adjuvant containing antioxidants and growth factors to be added to the culture medium of human mesenchymal stem cells to make human mesenchymal stem cells grow in the first generation Or subculture can rapidly proliferate and obtain growth factors. Background technique [0002] Press, the current scientific community has given some basic definitions and characteristics of stem cells: it refers to a group of cells that have the ability of self-renewal and proliferation, and can maintain the state of undifferentiation (Undifferentiation) for a long time, and The multi-differentiation characteristics of cell groups that can differentiate into different lineages and tissues with specific functions after being properly induced and stimulated. [0003] Depending on the source of stem cells and their differentiation potential, they...

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Problems solved by technology

However, the culture method revealed by it is to put somatic cells into a basal medium DMEM (Dulbecco`s Modified Eagle Medium) containing 10% fetal bovine serum (10% FBS) for culture. The secreted growth factors are relatively small. To obtain a large amount of growth factors, it is necessary to increase the number of days of culture and the number of generations of subculture. Therefore, there is also a risk of contamination due to multiple subcultures.

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