39results about How to "Avoid the risk of cross-contamination" patented technology

The invention provides a probe based on double-signal amplification triggered by a target and application of the probe. The probe comprises a first hairpin, a second hairpin and a third hairpin, wherein the first hairpin, the second hairpin and the third hairpin are formed by pairing hybridization of complementary bases in folding areas after single-chain linear molecules are folded back; the parts, forming double-chain structures in the partial areas, of the hairpins are stem areas, and the parts, not forming the double-chain structures and folded back, are ring-shaped areas. According to the probe provided by the invention, the self-assembly of hairpins is realized through target protein catalysis, signal amplification is achieved under the assistance of an excision enzyme III, obvious amplification of a detection signal is realized, and the detection sensitivity is greatly improved further; since the probe does not depend on template replication in the detection process, the problem that false positive occurs due to cross contamination in the detection process is avoided, generation of a false positive signal is effectively prevented and background noise is reduced greatly.

The invention relates to the technical field of in-vitro nucleic acid detection and particularly provides a primer group and kit for detecting polymorphic sites of aspirin-resistant related genes, andan application thereof. The primer group and the kit for detecting polymorphic sites of aspirin-resistant related genes and the kit can detect polymorphism of genes such as COX1, COX2, GPIIIa, PEAR1,P2Y1, GPIa and PAI-1; the sensitivity is high, so that genome DNA low to 0.1ng/muL can be accurately detected; the specificity is good, the genome DNA up to 300ng/muL can not generate non-specific amplification; the whole Fluorescence PCR detection process can be finished only within 90 minutes, and the detection result is intuitive and easy in interpretation.

The invention relates to the technical field of biological pharmacy, in particular to a culture assembly and a medicine screening biological reaction device. The culture assembly comprises a culture plate; a plurality of groups of micro-flow culture chambers are arranged on the culture plate; each group of micro-flow culture chambers comprises a plurality of pore chambers; a fluid communication channel is arranged between every two adjacent pore chambers; each fluid communication channel is provided with a circulation valve; each pore chamber is internally provided with a three-dimensional bracket matched with the pore chamber correspondingly; a fluid passing cavity is formed between the bottom of each three-dimensional bracket and the bottom of the corresponding pore chamber; a supporting bracket layer is arranged at the bottom of the three-dimensional bracket; the supporting bracket layer comprises a plurality of through holes, and an electrostatic spinning film is arranged on the supporting bracket layer; and a liquid inlet and a liquid outlet which are communicated with the fluid communication channel are formed in each group of micro-flow culture chambers correspondingly. According to the culture assembly, each micro-flow culture chamber channel is separated, the risk of cross contamination is avoided, and the form of the flow valve is adopted, so that liquid flows in an S-shaped wave form, and local stagnant water is avoided.

The invention is applicable to the technical field of lithium ion batteries, and provides a manufacturing method of a bipolar polymer lithium ion battery and a lithium ion battery. A bipolar pole piece, a single-side positive pole piece and a single-side negative pole piece are manufactured by utilizing a positive active material piece, a current collector and a negative active material piece through hot-pressing compounding and a rolling method, the obtained pole pieces adsorb a mixed solution containing an electrolyte solution, and the pole pieces are wrapped by a polymer film through ultraviolet irradiation; and rolling, punching and laminating are performed to obtain a pole group, and rolling, packaging and forming are carried out on the pole group to obtain the bipolar polymer lithiumion battery. The lithium ion battery manufactured by the manufacturing method provided by the invention has the advantages of high safety, small internal resistance, high charging and discharging power, wide working temperature range and long cycle life, and after the lithium ion battery is manufactured into a module and a system, a thermal management device can be simplified, and the overall system manufacturing cost is reduced.

The invention discloses a livestock and poultry farm vehicle intelligent decontamination system and an implementation method, and relates to the field of livestock and poultry breeding equipment. Thesystem comprises a cleaning unit, a draining and disinfecting unit, a drying unit, a personnel disinfecting unit, an article disinfecting unit, a wastewater collecting (treating) unit, a personnel resting unit, a vehicle transferring device, a license plate recognition camera, a livestock and poultry excrement recognition camera, a cleaning effect detecting device, a disinfecting effect detectingdevice and a control system. A vehicle passes through the cleaning unit, the draining and disinfecting unit and the drying unit in sequence to be decontaminated after being detected to be qualified, and the vehicle can enter the next step to be decontaminated after being detected to be qualified through cleaning and disinfecting effects. Personnel and articles are respectively decontaminated by the personnel and article disinfection unit, and the articles can be taken and used after the disinfection effect detection is qualified. Decontamination paths of vehicles, personnel and articles are independent of one another, and the decontamination operation can be synchronously carried out. The decontamination system is reasonable and compact in layout, standard in process, and efficient and intelligent in operation, and the decontamination effect is guaranteed.

The invention discloses a fully-closed pollution-free digital nucleic acid detection chip which comprises a chip body, a first puncture needle and a second puncture needle, the chip body is made of a transparent material, a plurality of oil inlet through holes are formed in the chip body, outlets of the oil inlet through holes are connected with an oil inlet groove, one end, connected with the first puncture needle, of the oil inlet groove is provided with an opening, an opening in the other end of the first puncture needle extends into a test tube, the test tube is used for receiving an oil phase conveyed by the oil inlet through holes, an opening in one end of the second puncture needle is connected into a liquid inlet groove formed in an inner cavity of the chip body, an outlet of the liquid inlet groove is connected with a tiling cavity, the bottom plane of the tiling cavity is in the horizontal direction, and an opening in the other end of the second puncture needle extends into the test tube; and the opening in the other end of the first puncture needle extends into the oil phase in the test tube all the time, and when the test tube is filled with the oil phase, the opening in the other end of the second puncture needle is in contact with the top liquid level of the oil phase. The risk of cross contamination is avoided, operation is easy, and consumed time is short.

The invention provides a method for extracting and purifying pravastatin sodium from a fermentation liquor, and belongs to the field of biological medicine preparation. According to the method for extracting and purifying pravastatin sodium from the fermentation liquor, a large amount of impurities and pigments are removed by alkalizing and acidifying the fermentation liquor, and the effective components in an acidizing liquid are enriched by an adsorbent, so that the purity of an extraction substrate is improved, the quantity of the extraction substrate is greatly reduced, and the quality ofan extraction liquid is further improved; in the whole production process, a single solvent system is adopted, the process is not complicated, the generated three wastes are low in amount, and the method is suitable for large-scale production. The content of pravastatin sodium produced by the method is more than 98.8 percent, the content of main impurity 6-epipravastatin is less than 0.15%, the content of total related substances is less than 0.4%, the product quality accords with the EP9.6 edition quality standard (the content of the pravastatin sodium is between 97.0% and 102.0%; a main impurity A, namely6-epipravastatin, is less than or equal to 0.3%; the content of the total related substances is less than or equal to 0.6%) of qualified pravastatin sodium, the total yield can reach more than 75%, and the large-scale production prospect is good.

The invention relates to the field of medical equipment and especially relates to a disposable anti-pollution and anti-leakage syringe, and the syringe comprises a needle cylinder, a piston matching with the needle cylinder, and a push rod connected with the piston, wherein the syringe further comprises a sealing sleeve which is sleeved on the push rod, one end of the sealing sleeve is in closed connection with that of the needle cylinder opening, and the other end of the sealing sleeve is in closed connection with the piston or the push rod. In comparison with the traditional syringe, the syringe can effectively avoid germs from outside air or hands of medical personnel to enter inside solution so as to further prevent from bacterial infection and cross infection.

The invention provides wafer sorting equipment and a wafer sorting method. The equipment comprises a sorting chamber, wherein the sorting chamber comprises a first layer chamber and a second layer chamber, a first discharging port is formed in the discharging end of the first layer chamber, and a first conveying belt is arranged in the first layer chamber, a second discharging port is formed in the discharging end of the second layer chamber, and a second conveying belt is arranged in the second layer chamber, a filtering fan unit is arranged above the sorting chamber; and a calibrator unit, wherein the calibrator unit is located at the feeding end of the sorting chamber, the calibrator unit is at least provided with a first feeding port and a second feeding port, at least two mechanical arms are arranged in the calibrator unit, a first mechanical arm is used for transferring OK wafers from the first feeding port to the first conveying belt of the first layer chamber, and a second mechanical arm is used for transferring NG wafers from the second feeding port to the second conveying belt of the second layer chamber. According to the wafer sorting equipment and the wafer sorting method, the environment cleanliness during wafer sorting can be improved, the pollution risk is reduced, and the efficiency is improved.

The invention relates to the field of laboratory containers, in particular to a test tube capable of transferring a liquid at the upper end and a test tube assembly. The test tube comprises a tube body, wherein the lower end of the tube body is a closed tube bottom, a tube opening is formed in the upper end of the tube body, a wall bulge protruding towards the radial outer side of the tube body isarranged below the tube opening of the upper part of the tube body, and the wall bulge is provided with a concave cavity for allowing the liquid in the test tube to flow into the test tube when beingoverturned to a low position. The test tube assembly comprises the test tube capable of transferring the liquid at the upper end and a flip type tube cap which covers the upper end including the wallbulge of the test tube. According to the scheme, the problems of inconvenience caused for the reason that when the existing test tube is used, a pipette or a suction head needs to be inserted into the tube bottom to transfer the liquid and cross contamination which is easily caused for the reason that a liquid-transferring machine rod is inserted into the tube body can be solved, and the flip type test tube assembly can avoid the risk of falling and mismatch of a split type tube cap, and is convenient to operate.

The invention provides an intelligent tableware disinfecting cabinet. The disinfecting cabinet consists of an upper cover, a cabinet body and an intelligent control system; the upper cover includes aautomatic human body inductor, a flip cover and a flip cover driving mechanism; the cabinet body includes an ozone generator, electric heating pipes and a tableware storage and elevating mechanism; and the intelligent control system includes temperature control, ozone concentration control, elevating mechanism start-stop control, elevating mechanism motion directional control and flip cover open-close control. When the automatic human body inductor inducts that someone takes tableware, the flip cover can automatically opened and closed after the tableware are taken away; when the tableware reserved at a tableware taking position is used up, the elevating mechanism can automatically push out the tableware from the cabinet body to supplement; and when all the tableware in the cabinet body are used up, the addition of the tableware can be prompted by a working status indicator, and a tableware position sensor can control the elevating mechanism to cooperate with an operator to add the tableware in a gradual declining manner. The disinfecting cabinet is mainly used for the sterilization, disinfection and storage of the tableware, is suitable for various tableware such as plates, bowlsand trays and has the advantages of being convenient in tableware taking and simple in equipment operation; and the problems of hand cross contamination during the opening of a door can be avoided when people take the disinfected tableware, so that the disinfecting cabinet is especially suitable for public dining places such as buffet restaurants, fast food chain stores, hotels and unit canteens.

A D-dimer tester comprises a rotary sample application arm, a reagent position, a test position, a double tube pump, a liquid collection tube, a sample position, a microinjection pump and a needle washing position, wherein the sample position, the reagent position, the test position and the needle washing position are in a same working platform. The D-dimer tester provided by the invention is easy to operate, avoids the risk of cross contamination, has high consistency of test results, stable test and high precision, and meets market demands; and the unique alarm function of the D-dimer tester can avoid errors in manual operation, so as to ensure the correctness of test results in a hospital.

The invention relates to the technical field of medical instruments, and particularly discloses a soaking and disinfection device for a rigid endoscope. The device comprises a shell, wherein a cleaning barrel is arranged in the shell, a liquid outlet and an inserting opening allowing the rigid endoscope to be inserted are formed in the cleaning barrel, a drainage pipe is connected to the liquid outlet, a drainage valve is arranged on the drainage pipe, and a heating piece is arranged on the cleaning barrel; and a clean water barrel, an enzyme solution barrel and a disinfectant barrel which allcommunicate with the cleaning barrel are arranged in the shell, a first pump body communicates between the clean water barrel and the cleaning barrel, a second pump body communicates between the enzyme solution barrel and the cleaning barrel, and a third pump body communicates between the disinfectant barrel and the cleaning barrel. According to the scheme, the problem that in the prior art, whenthe rigid endoscope is taken out of a rigid endoscope cleaning disinfection machine and stored after cleaning and disinfection, the rigid endoscope is likely to be polluted is solved.

The invention provides a filter device used for a micro device. The filter device is used for filtering a liquid flowing into the micro device. The filter device comprises a filter and a connecting part, wherein the filter is fixed onto the micro device, and comprises an input end and an output end; the connecting part is used for connecting the output end of the filter and the first end of the micro device; the liquid flows into the filter from the input end of the filter, flows out from the output end of the filter, and then flows into the micro device after flowing through the connecting part. After the technical scheme is adopted, integrated integration of the filter device and the micro device is realized, and the filter device is beneficial for volume production; the filter device isconvenient to install and maintain, the service life of the micro device is prolonged, and the cost is reduced; the filter device does not exceed the original size of the micro device, and is convenient to use in narrow working environments; the cross contamination risk of a large device in the prior art is avoided.

The invention belongs to the field of nuclear fuel after-treatment technology and particularly relates to a mechanical arm separation module for an automatic separation device of nuclear samples. Themechanical arm separation module comprises a reagent storage groove bracket, a separation column bracket, a waste liquid treatment assembly, a slide rail, a mechanical arm platform, a sample bottle bracket and a lifting plate. The mechanical arm separation module is provided with five moving shafts, including three shafts for helping a sampling probe to complete movements in X-axis, Y-axis and Z-axis directions, the lifting plate for enabling the sample bottle bracket to move in the Z-axis direction, and the slide rail for enabling the separation column bracket to move in the X-axis direction,and complicated separation processes can be completed by perfect cooperation of the five shafts, so that the used space of the inside of a glove box is reduced, repair and maintenance are facilitated, the flowing time of reagents and samples is shortened, the liquid sampling accuracy is improved, the separation time is shortened, the efficiency is improved, and the function of performing sample loading separation twice after dilution is performed on eluent once is realized.