Fully-closed pollution-free digital nucleic acid detection chip and detection method

A detection chip, non-polluting technology, applied in chemical instruments and methods, biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of complicated pipetting operations, time-consuming cross-contamination, etc., to avoid reaction throughput , to avoid droplet fusion and facilitate real-time observation

Active Publication Date: 2021-12-28
XI AN JIAOTONG UNIV
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Problems solved by technology

[0007] In order to solve the problems existing in the prior art, the present invention provides a fully enclosed and non-polluting digital nucleic acid detection chip and detection method, which solves the current problem of complicated liquid droplet pipetting operation, time-consuming and prone to cross-contamination

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  • Fully-closed pollution-free digital nucleic acid detection chip and detection method
  • Fully-closed pollution-free digital nucleic acid detection chip and detection method
  • Fully-closed pollution-free digital nucleic acid detection chip and detection method

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Embodiment Construction

[0055] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0056] Such as figure 2 and image 3 As shown, the present invention provides a fully enclosed and pollution-free digital nucleic acid detection chip, which separates PCR amplification from subsequent detection, reduces the complexity of the chip, avoids the restriction of the detection process on the reaction flux of PCR amplification relief, and can Quickly and effectively promote the flattening of droplets, no need for two oil phases, fewer chip consumables, and lower cost, especially the integrated chip avoids the risk of cross-contamination that may be caused by opening the cap during the pipetting process, and is relatively Specific flow detection can observe the state of micro-droplets, improve the detection success rate and timeliness, and can also be applied to proteins or nucleic acids, such as PCR\LAMP\RPA reactions and other techn...

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Abstract

The invention discloses a fully-closed pollution-free digital nucleic acid detection chip which comprises a chip body, a first puncture needle and a second puncture needle, the chip body is made of a transparent material, a plurality of oil inlet through holes are formed in the chip body, outlets of the oil inlet through holes are connected with an oil inlet groove, one end, connected with the first puncture needle, of the oil inlet groove is provided with an opening, an opening in the other end of the first puncture needle extends into a test tube, the test tube is used for receiving an oil phase conveyed by the oil inlet through holes, an opening in one end of the second puncture needle is connected into a liquid inlet groove formed in an inner cavity of the chip body, an outlet of the liquid inlet groove is connected with a tiling cavity, the bottom plane of the tiling cavity is in the horizontal direction, and an opening in the other end of the second puncture needle extends into the test tube; and the opening in the other end of the first puncture needle extends into the oil phase in the test tube all the time, and when the test tube is filled with the oil phase, the opening in the other end of the second puncture needle is in contact with the top liquid level of the oil phase. The risk of cross contamination is avoided, operation is easy, and consumed time is short.

Description

technical field [0001] The invention belongs to the technical field of microfluidic droplet digital polymerase chain reaction, in particular to a fully enclosed and pollution-free digital nucleic acid detection chip and a detection method. Background technique [0002] With the transformation of the medical model and the continuous development of individualized medicine, the medical laboratory urgently needs fast and accurate detection methods, and molecular detection has unique advantages. At present, molecular detection technologies mainly include nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochip technology. Centers, technical service centers, third-party testing agencies, and rapid microbial testing markets, etc., and play an important role in the detection of new coronaviruses. Digital PCR (Digital PCR, dPCR) technology is based on single-molecule PCR method to count the nucleic acid quantification, is a nucleic acid molecule amplificatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12M1/24C12M1/00B01L3/00C12Q1/6851
CPCB01L3/5027C12Q1/6851B01L2200/10B01L2200/027C12Q2531/113C12Q2563/159C12Q2563/107C12Q2565/629Y02A50/30
Inventor 彭年才梁焱赵书豪胡飞张增明姜颖涛刘振李希晨路朝昌郭晓牛
Owner XI AN JIAOTONG UNIV
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