The invention provides a probe based on double-signal amplification triggered by a target and application of the probe. The probe comprises a first hairpin, a second hairpin and a third hairpin, wherein the first hairpin, the second hairpin and the third hairpin are formed by pairing hybridization of complementary bases in folding areas after single-chain linear molecules are folded back; the parts, forming double-chain structures in the partial areas, of the hairpins are stem areas, and the parts, not forming the double-chain structures and folded back, are ring-shaped areas. According to the probe provided by the invention, the self-assembly of hairpins is realized through target protein catalysis, signal amplification is achieved under the assistance of an excision enzyme III, obvious amplification of a detection signal is realized, and the detection sensitivity is greatly improved further; since the probe does not depend on template replication in the detection process, the problem that false positive occurs due to cross contamination in the detection process is avoided, generation of a false positive signal is effectively prevented and background noise is reduced greatly.