The invention relates to a lansoprazole frozen powder injection agent and a preparation method thereof. The lansoprazole frozen powder injection agent prepared by the method can be taken as a medicine for treating gastric ulcer, duodenal ulcer, erosive gastroesophageal reflux disease, helicobacter pylori and Zollinger-Ellison Syndrome. The lansoprazole frozen powder injection agent prepared through taking a lansoprazole as an active ingredient, adopting crystallized filtration, freezing and drying. The preparation process comprises the following steps of: adding water for injection and alkali to the lansoprazole, stirring to dissolve and mix uniformly; performing the crystallized filtration; after measuring the content, adjusting pH value of filtrate; using the water for injection for fixing volume and a millipore filter with 0.22 mu m for filtration; filling, partially arranging corks, arranging in trays, freezing out, pressing the corks, taking out from boxes, rolling openings, checking quality and packaging. The prepared lansoprazole frozen powder injection agent has excellent re-dissolving performance, is more stable when used together with transfusion in clinical use, can not separate out, and has better inspection result for visible foreign matters and insoluble particles.