35results about How to "Optimize extraction conditions" patented technology

The invention discloses pumpkin polysaccharide and the preparation method thereof. The preparation method comprises the steps as follows: pre-treating pumpkin, extracting in water for 0.5-3h, filtering to obtain filtrate, centrifuging the filtrated supernatant, purifying the supernatant with a resin column chromatography, and colleting eluent; concentrating the eluent, and precipitating with ethanol; and dissolving the precipitate into water, adding trichloroacetic acid, centrifuging for deproteinization to obtain supernatant, concentrating the supernatant, drying and pulverizing. The washing number of times is reduced, the technological process is simplified, and the extraction condition and technological parameter are optimized to shorten the time of the extraction and purification processes by purifying the pumpkin polysaccharide with advanced resin chromatography technology. Therefore, the preparation method has the advantages of simple technological process, short production period, easily-controlled production, and low production cost. The purity of the obtained pumpkin polysaccharide is as high as more than 80%, and the extraction rate is as high as more than 25%.

The invention discloses a method for analyzing the content of adenosine and cordycepin in the sporocarp of cordyceps militaris through artificial culture by virtue of high performance liquid chromatography (HPLC). The method comprises the following specific steps: 1, weighing the dried sporocarp powder of cordyceps militaris in a 50ml centrifuge tube with a plug, adding water, ultrasonically extracting the centrifuge tube in water bath, then centrifuging and taking a supernate as a test solution; 2, weighing a certain amount of standard substances of adenosine and cordycepin, adding water and dissolving to prepare a mixed solution as a mixed standard solution; 3, performing content measuring, namely taking the mixed standard solution and test solution respectively, filtering the solutions through a 0.45um aqueous phase millipore filter and injecting the filtered solutions into a small bottle, and measuring the chromatographic peak of the cordyceps militaris sporocarp test solution and the adenosine cordycepin standard solution with the same chromatographic retention time. The invention provides a new, simple and convenient method for evaluating the quality of the cordyceps militaris sporocarp.

The invention discloses a method for rapidly measuring a content of bongkrekic acid in food by automatic solid phase extraction-ultra performance liquid chromatography. The method comprises steps of pretreatment of food samples, purification of extracted solution and HPLC analysis to obtain the content of bongkrekic acid in the food samples; and results are proved. The method provided by the invention replaces a traditional manual column passing method with an automatic solid phase extraction instrument to realize automation, reduce labor costs and personal errors, realize controllability of solid phase extraction velocity, improve repeatability and accuracy of experiments and shorten a sample treatment cycle; the method optimizes extraction conditions, chromatographic conditions and the like to obtain an excellent separation degree and chromatographic peak, has advantages of high sensitivity, high recovery rate and relatively short pretreatment cycle, meets requirements on detection of the content of bongkrekic acid in food, provides a reference for a laboratory to carry out relevant detections and plays an active role in ensuring food safety.

The invention relates to a method for extracting total saponins from bark of acanthopanax brachypus harms, and belongs to the field of extraction of active ingredients of natural products. The process flow is that the bark of acanthopanax brachypus harms is taken as raw materials, the mixed solvent of [Bmin]Br and water is taken as extraction solvent, continuous flowing extraction is performed through ultrasonic-microwave collaborative solvent, extraction process is detected through an IR detector, and filter liquor is deposited and centrifuged to obtain clear extracting solution; and the extracting solution is degreased through diethyl ether and is subjected to macroporous absorption resin column chromatography, so that 70% of ethanolic elution is collected, discolored through activated carbon, and impurities are removed through aluminum oxide so as to obtain total saponins. After analysis, the extraction rate achieves more than 8.56%, the content of the total saponins achieves more than 92.53%, and is higher than that of the total saponins obtained by other methods. The method has the advantages of normal pressure extraction, simple process, quickness and simplicity in operation, energy conservation and environmental friendliness and high selectivity, and can perform on-line detection to the extraction process; the saponin is not easy to denature, and does not need to be filtered and separated after extraction; and the extraction rate and purity of the total saponins are high, and the industrialized production can be facilitated.

The invention discloses a method for extracting mitochondrial deoxyribonucleic acid (DNA) of cotton. The method comprises the following steps of: (1) homogenizing cotton, and centrifuging and collecting precipitate; (2) removing nuclear DNA, adding ethylene diamine tetraacetic acid (EDTA) solution, and centrifuging to obtain the precipitate; (3) adding buffer solution, and centrifuging to obtain mitochondria; (4) adding lysis solution and acetate solution, centrifuging, collecting supernatant, and extracting by using chloroform-isoamylol; (5) adding acetate solution and absolute ethanol, centrifuging to obtain the precipitate, and washing by using ethanol; (6) dissolving in triisopropylphenylsulfonyl (Tris)-EDTA (TE); (7) removing ribonucleic acid (RNA) and protein, extracting by using Tris saturated phenol-chloroform-isoamylol, and centrifuging and collecting the supernatant; and (8) adding the acetate solution and the ethanol, and centrifuging to obtain the precipitate; and washing by using the ethanol to obtain the mitochondrial DNA. Aiming at defects of the conventional extraction of the mitochondrial DNA, the conventional cetyltrimethylammonium bromide (CTAB) method is improved, and the extraction conditions are optimized by adjusting the time and times of centrifugation, changing reagents, increasing extraction and washing times, and the like. On the basis of ensuring that the mechanical damage degree of the mitochondria is minimum, the nuclear DNA, phenols and polysaccharides outside the mitochondria are effectively removed, pollution rate is reduced, the purity and the quality of the mitochondrial DNA are improved, and the mitochondrial DNA can meet the requirement of subsequent experiments of the gene engineering of the cotton mitochondria.

The invention discloses a method for detecting n-propyl bromide in leather and textiles. The method is characterized in that the gas chromatography-mass spectrometry combination is used for detection. The detection method is simple, convenient and fast and has high sensitivity. A sample to be detected is in the linear relationship in the range of 0.1-50mg / L, the correlation coefficient is 0.9995, the method detection limit is 0.1mg / L, the standard recovery rate is 96%-112%, and the relative standard deviation (RSD) (n is equal to 7) is less than 2%. The method can be used for detecting the n-propyl bromide in the leather and textiles very well.

The invention relates to the field of nucleic acid extraction, in particular to a method for direct extraction of nucleic acid without tissue grinding. In order to solve the defects of burdensome operation, long extraction cycle, no realization of automation, contact of toxic reagents and the like, and the technical problems that in the extraction process of a novel nucleic acid extraction instrument, animal tissue samples need grinding, cracking and centrifugation in advance and cannot achieve direct extraction on a machine, the invention provides a method for direct extraction of nucleic acid without tissue grinding. The method adopts a unique cracking reagent and employs a 96-channel nucleic acid extraction instrument for support to realize integration of instrument and consumables, and achieves full-automatic extraction of animal tissue nucleic acid. The reagent is optimized cracking liquid for different samples, and the instrument is nucleic acid extraction instrument, and the extraction conditions can be optimized according to different tissue samples. The method provided by the invention has the advantages of simple steps, full automatic operation, high purity of nucleic acid, short extraction cycle, and no contact with toxic reagent, etc.

The invention relates to an efficient method for extracting lutein from Cordyceps militaris, which comprises the following steps: preparing raw materials, performing fermentation treatment, performing ionic liquid extraction and purifying. By performing optimal combination on the extraction process and creatively adopting an ionic liquid / assistant composite extraction manner, the method achieves the purpose of efficiently extracting lutein from Cordyceps militaris, obtains the technical effect that the extraction rate is far higher than that in the prior art, greatly increases the utilization ratio of the raw materials, and avoids the consumption of a large amount of organic solvent. Thus, the method has the advantages of being green, environment-friendly and suitable for large-scale production, and has an application prospect of being widely industrialized.

The invention discloses a curcumin extraction and purification method which comprises the following steps: weighing dry turmeric powder, adding an ethanol aqueous solution with the volume fraction ofgreater than or equal to 74%, and performing three times of extraction and three times of filtration, with a recycling extraction manner adopted; diluting the primary extraction filtrate until the ethanol content does not exceed 50%, and performing sample loading adsorption through a polyamide resin chromatographic column; washing the column with purified water of which the volume is three times that of the column to remove water-soluble impurities; washing the column with an ethanol aqueous solution with the volume fraction of 50%-70% to remove alcohol-soluble impurities; washing the column with an ethanol aqueous solution which is 5 times the volume of the column and which has a volume fraction of 60-75% to elute curcumin on the column, and collecting eluate; concentrating the collectedeluate to be dry, adding an ethanol aqueous solution to dissolve to obtain a saturated solution, crystallizing and separating out at 0 DEG C to room temperature, washing the crystals, and drying to obtain curcumin. The extraction and purification process is simple, the extraction and purification processes are easy to control, industrial application is easy, the turmeric extract with the total curcumin content larger than or equal to 95% can be prepared, and economic benefits are good.

The invention discloses an extraction method of polygonatum polysaccharide. The extraction method includes the steps that S1, dried polygonatum polysaccharide decoction pieces are weighed and crushed,and distilled water is added for soaking for 24 hours; S2, distilled water is added according to a certain material-to-liquid ratio, and centrifuging and concentrating are carried out after refluxingextraction; S3, 95% ethanol is added into a concentrate, putting overnight is carried out, and centrifugation and freeze-drying are carried out to obtain crude polysaccharide; S4, the polygonatum crude polysaccharide is taken, dissolved, mixed with chloroform-n-butanol, shaking vigorously is carried out for 30 minutes, protein is denatured to form a gel, and centrifuging is carried out to removethe denatured protein in a middle layer; and S5, activated carbon is added to a polygonatum polysaccharide solution, decolorizing is carried out, stirring is carried out once every 10 minutes, add absolute ethanol is added into an extract solution to enable the ethanol concentration in the mixed solution reach 80%, standing overnight is carried out, and centrifuging and freeze-dry are carried outto obtain the polygonatum polysaccharide. The extraction method is simple to operate, economical and feasible; and through a plurality of extractions, the extraction method has the advantages of higher extraction rate and less impurities.

The invention discloses a method for extracting and purifying physalis polysaccharides by using ultrasonic waves, and belongs to the field of preparation of extracted polysaccharides. The preparation method comprises the following steps: washing, beating, drying and pulverizing the physalis and passing through a 60-mesh sieve to obtain the physalis powder; after ultrasonic treatment under different conditions, centrifuging, and the supernatant is the polysaccharide extract. Through the single factor test, the optimum ratio of liquid to material, ultrasonic temperature, ultrasonic time, and ultrasonic power for extracting physalis polysaccharides were determined. Volume ratio of sample solution-sevage reagent, volume ratio of chloroform-n-butanol, shaking time, deproteinization times. Hydrogen peroxide decolorization, through a single factor test, finally determined the optimum decolorization temperature, solution pH value, decolorization time, hydrogen peroxide addition amount.

The invention aims to provide a preparation method of a cerebroside carnosine injection with good stability. The preparation method of a rabbit muscle extract for preparing the cerebroside carnosine injection comprises the following steps: mincing pretreated rabbit muscles into small blocks with the volume not higher than 1cm < 3 >; and uniformly mixing the obtained minced muscles with water for injection according to a ratio of 1: (1-3) (w / w), performing homogenizing, freezing the obtained muscle homogenate at a temperature not lower than-10 DEG C for 5-7 days, and performing melting at a temperature of 8-10 DEG C for 36-48 hours. According to the method, the extraction efficiency of the polypeptide in the rabbit muscle extract is remarkably improved, the risk that the rabbit muscle and the rabbit muscle extract are infected by microorganisms is effectively avoided, the freshness of the rabbit muscle extract is remarkably reduced, and the resource utilization rate of the rabbit muscle, the quality of drugs, the quality uniformity, the effectiveness and the safety are remarkably improved.

The invention discloses a method for extracting EGCG (epigallocatechin gallate) in tea leaves by water extraction; according to the method, the GCG in the tea leaves is extracted with de-ionized water, and on the basis of a single factor experiment, the extraction condition of the EGCG is optimized by using a response surface method, so as to achieve the optimal extraction condition that the pH (potential of hydrogen) of the de-ionized water is adjusted to be 6, the liquid-material ratio is 41, namely 41ml of de-ionized water is added in per gram of green tea, the mixture is extracted for 34min in a constant-temperature thermostat water bath of 85 DEG C and then is filtered, so as to obtain an EGCG extract, the extraction is repeated for once, and then the EGCG content is detected, wherein the yield of the EGCG can reach 3.40%.

The invention belongs to the technical field of deep processing of agricultural products, and discloses a method for simultaneously preparing shiny-leaved yellowhorn oil and shiny-leaved yellowhorn protein through an aqueous enzymatic method. The method comprises the steps: obtaining slurry; carrying out enzymolysis and centrifugation; carrying out ultrasonic demulsification treatment to obtain free oil; and sequentially carrying out hydration degumming, deacidification and decoloration treatment to obtain the high-purity shiny-leaved yellowhorn oil; adding alkaline protease into a primary crude protein liquid, and carrying out alkaline extraction two times; obtaining a secondary alkali extract solution, carrying out centrifugal separation on the obtained secondary alkali extract solutionto obtain a secondary protein liquid; and adjusting the pH value, carrying out acid precipitation centrifugation, and carrying out freeze drying to obtain the shiny-leaved yellowhorn protein. The process is simple and safe, and the extracted shiny-leaved yellowhorn oil is strong in fragrance, light in color and good in quality; the prepared shiny-leaved yellowhorn protein is higher in purity, theprotein content is higher than 50%, and the shiny-leaved yellowhorn protein is suitable for industrial production. According to the method, the oil yield is increased, meanwhile, no pollution is caused, the safety is high, and a combustible solvent is not used, so that the possibility of flammability and explosiveness is reduced, the pollution is controlled, and the safety is guaranteed; the costis low.

The invention belongs to the technical field of Mongolian medicine, and the method comprises the following steps of: preparing Sug Mel-4 soup; preparing a rutin reference solution; selecting the maximum wavelength; drawing a standard curve; single factor experiment: taking the flavone content as an indicator, and observing the influence of a liquid-to-liquid ratio, an extraction time and an extraction temperature on the flavone content by using a single factor method; response surface method optimization process; and orthogonal method optimization process verification: using time A, temperature B, and material-liquid ratio C as experimental factors to perform an orthogonal design experiment. The extraction conditions are optimized by using the response surface and orthogonal design experiments, the influence of the extraction time, the extraction temperature and the liquid-to-liquid ratio on the flavone content in the Sug Mel-4 soup is observed, the flavone yield in the Sug Mel-4 soupis used as the indicator, the optimal process conditions are that the material-liquid ratio is 1:40, the time is 120min, the temperature is 100 DEG C, it is simple, convenient, feasible, stable and reliable to increase the flavone content in the Sug Mel-4 soup by using a water bath heating reflux method, and the method is suitable for industrial production.

The invention relates to an efficient method for extracting lutein from Cordyceps militaris, which comprises the following steps: preparing raw materials, performing fermentation treatment, performing ionic liquid extraction and purifying. By performing optimal combination on the extraction process and creatively adopting an ionic liquid / assistant composite extraction manner, the method achieves the purpose of efficiently extracting lutein from Cordyceps militaris, obtains the technical effect that the extraction rate is far higher than that in the prior art, greatly increases the utilization ratio of the raw materials, and avoids the consumption of a large amount of organic solvent. Thus, the method has the advantages of being green, environment-friendly and suitable for large-scale production, and has an application prospect of being widely industrialized.

The invention discloses a method for separating and purifying T lymphocytes. The method comprises the following four steps: 1) separating plasma; 2) separating lymphocytes; 3) cleaning lymphocytes; 4)purifying lymphocytes. According to the technical scheme of the invention, extraction conditions of T lymphocytes are optimized and effective utilization of T lymphocytes is benefited.

The invention belongs to the field of medicines and in particular relates to a traditional Chinese medicine for treating Parkinson's disease aiming at syndromes of liver-kidney yin deficiency and phlegm stagnation derived from same fountainhead, and a preparation method thereof. The traditional Chinese medicine is prepared from the following raw materials: radix rehmanniae, prepared rehmannia root, fructus corni, ophiopogon root, schisandra, herba epimedii, curculigo rhizome, silkworm moth, herba cistanches, morinda root, radix adenophorae, tortoise plastron, phellodendron bark, dangshen, lycopodium clavatum, agrimony, oyster, pangolin, rhizoma pinelliae preparata, deer-horn glue, glue of tortoise plastron, pseudo-ginseng powder, licorice and honey. Researches on medicinal material extraction, preparation processes, quality standards and the like of the traditional Chinese medicine are carried out according to the application requirements of new medicines, thereby ensuring the medication safety and effectiveness of people.

The invention provides a method for extracting gastrodin by an enzyme method. The method is characterized by comprising the following steps: dissolving rhizoma gastrodiae powder in acetic acid-sodium acetate buffer solution and mixing the two substances evenly; adding complex enzyme into the solution for stirring evenly and enzymolysis; adding alcohol into the mixture to for sufficiently stirring, filtering and deslagging; concentrating extracting solution into extract by a rotary steaming method; and drying the extract to obtain the gastrodin extract. The method has the advantages that as high efficiency and specificity of biological enzyme and mild acting condition, compared with an ultrasonic extracting process, the extracting condition is more convenient, and the equipment is more simplified, so that energy consumption is reduced, and the cost is saved; compared with a hot alcohol refluence extracting process, the extracting condition is simple, the temperature of an extracting system is reduced, so energy consumption of heating is reduced, the pollution to the environment is reduced, a micromolecular drug effect ingredient with thermal volatilization can be remained, and the drug effect of the obtained gastrodin is more complete; and compared with the prior art, the extracting rate is improved by 0.05 to 0.13 percent.

The invention relates to a pollution-free integrated method and device for recycling extracellular polymers with high added value. Wherein, the method includes the following steps: step S1: transport the excess sludge to a cation exchange resin reactor, and extract extracellular polymers in the excess sludge; step S2: intercept the microbial cells and their components in the excess sludge through the microfiltration membrane module Residue, obtain the filtrate containing extracellular polymers; Step S3: Filtrate through multi-stage membrane modules, which can sequentially concentrate and purify high molecular weight extracellular polymers and low molecular weight extracellular polymers, and then separate different molecular weight range of extracellular polymers; step S4: the filtrate produced by the filtration of step S3 is transported to the forward osmosis membrane module using fertilizer as the driving agent for filtration to obtain a drawing solution containing fertilizer, which can be directly used for Water and fertilizer integrated irrigation or soilless cultivation. The invention has the characteristics of no damage to cells and no pollution.

The invention discloses a method for extracting mitochondrial deoxyribonucleic acid (DNA) of cotton. The method comprises the following steps of: (1) homogenizing cotton, and centrifuging and collecting precipitate; (2) removing nuclear DNA, adding ethylene diamine tetraacetic acid (EDTA) solution, and centrifuging to obtain the precipitate; (3) adding buffer solution, and centrifuging to obtain mitochondria; (4) adding lysis solution and acetate solution, centrifuging, collecting supernatant, and extracting by using chloroform-isoamylol; (5) adding acetate solution and absolute ethanol, centrifuging to obtain the precipitate, and washing by using ethanol; (6) dissolving in triisopropylphenylsulfonyl (Tris)-EDTA (TE); (7) removing ribonucleic acid (RNA) and protein, extracting by using Tris saturated phenol-chloroform-isoamylol, and centrifuging and collecting the supernatant; and (8) adding the acetate solution and the ethanol, and centrifuging to obtain the precipitate; and washing by using the ethanol to obtain the mitochondrial DNA. Aiming at defects of the conventional extraction of the mitochondrial DNA, the conventional cetyltrimethylammonium bromide (CTAB) method is improved, and the extraction conditions are optimized by adjusting the time and times of centrifugation, changing reagents, increasing extraction and washing times, and the like. On the basis of ensuring that the mechanical damage degree of the mitochondria is minimum, the nuclear DNA, phenols and polysaccharides outside the mitochondria are effectively removed, pollution rate is reduced, the purity andthe quality of the mitochondrial DNA are improved, and the mitochondrial DNA can meet the requirement of subsequent experiments of the gene engineering of the cotton mitochondria.

The invention discloses a method for detecting residues of eight organic matters in ion exchange resin by adopting a headspace gas phase method, which comprises the following steps of: performing ultrasonic extraction on a sample by using dimethyl sulfoxide by using a chemical analysis technical method, optimizing an extraction method and chromatographic conditions of the sample, and performing baseline separation and statistics on the eight residues; and enabling the linear correlation coefficients in the investigated concentration range to be all 0.999 or above, obtaining the lowest detection limit is obtained. The method is simple, accurate and reliable, and is suitable for determining the organic residual organic solvent in the adsorption resin.

The invention discloses a method for detecting n-propyl bromide in leather and textiles. The method is characterized in that the gas chromatography-mass spectrometry combination is used for detection. The detection method is simple, convenient and fast and has high sensitivity. A sample to be detected is in the linear relationship in the range of 0.1-50mg / L, the correlation coefficient is 0.9995, the method detection limit is 0.1mg / L, the standard recovery rate is 96%-112%, and the relative standard deviation (RSD) (n is equal to 7) is less than 2%. The method can be used for detecting the n-propyl bromide in the leather and textiles very well.

The invention relates to the technical field of preparation of beverages, in particular to a method for preparing a cucumber extract. The method comprises the following steps of (1) taking fresh cucumbers, thoroughly cleaning the taken fresh cucumbers, adding water which is 18-26 times of the cucumbers, and performing mashing so as to obtain paste; (2) putting the paste to a magnetic field of which the magnetic field intensity is 3000-3400Gs, and performing magnetisation under the temperature of 45-55 DEG C so as to obtain magnetised paste; (3) performing microwave extraction under the power of 600-700W on the magnetise paste for 30-50s so as to obtain a cucumber extracting solution; (4) uniformly mixing the cucumber extracting solution with honey and table salt in the weight ratio of the cucumber extracting solution to the honey to the table salt being (90-120) to (5-12) to (0.001-0.01); and (5) performing filtering, performing instantaneous sterilizing at ultrahigh temperature, and performing filling so as to obtain the cucumber extract. Through the adoption of the method disclosed by the invention, the efficacy of reducing weight of the cucumber extract can be improved.

The present invention provides process of utilizing kelp comprehensively and extracting fucoidin. The process has secondary soaking of kelp to raise the leaching rate of iodine, mannitol and fucoidin and to lower algin extracting cost. The water after enhanced stirring is flocculated to purify, and this can improve the iodine and mannitol extracting condition for high product yield and simplify post purification of fucoidin. The process of the present invention has improved product extracting condition, lowered production cost, obviously raised iodine, mannitol and fucoidin extracting rate and raised kelp resource utilizing value.

The invention discloses an extracting method of aluminum. Basic compounds and fly ash are adopted for forming, and sintering is performed; then a sintered sample block is subjected to acid leaching, and aluminum-containing crude liquid is obtained; the roasting temperature and the temperature and time of acid leaching are greatly lowered; the lowest temperature can reach 50-60 DEG C, and the efficiency of aluminum extraction reaches up to 88%; the SiO2 content in leached slag reaches 95%, and aluminum and silicon separation is achieved; due to agglomeration treatment, a porous structure of a mesoscale is molded for a reaction; the distance between two phases of a solid-liquid ratio is shortened, the mass transfer condition of a gas-liquid two phase is also effectively improved, and therefore extraction of aluminum is reinforced; and fly ash particles and capillary channels in the block sample play a framework role similar with coke in a blast furnace, and the reason that the aluminum oxide extracting rate is high due to an agglomeration means can be explained from the aspect of dynamics.

The present invention relates to the technical field of drink preparations and especially relates to a preparation method of cucumber dews. The preparation method comprises the following steps: 1) fresh cucumbers are taken, the taken cucumbers are washed clean, water at 20-30 times of the weight of the cucumbers is added, and the washed cucumbers are beat into slurry; 2) the slurry is put into a magnetic field at a magnetic field strength of 3000-3400 Gs to conduct magnetization for 10-20 min to obtain a magnetized slurry; 3) then the magnetized slurry is subjected to an ultrasonic extraction for 20-30 min at a power of 400-500 W to obtain an extract; 4) the cucumber extract is subjected to a double filtration, the filtered extract is clarified, the clarified extract is subjected to a centrifugal separation to obtain cucumber raw juice; and 5) the cucumber raw juice is subjected to an ultra-high temperature instantaneous sterilization, and the sterilized mixture is filled to obtain the cucumber dews. The prepared cucumber dews by the method can improve the anti-aging effects of the cucumber dews.

The invention relates to a pollution-free integrated method and device for high-added-value extracellular polymeric substance recovery. The method comprises the following steps: S1, conveying excess sludge into a cation exchange resin reactor, and extracting extracellular polymeric substances in the excess sludge, S2, intercepting microbial cells and residues thereof in the excess sludge through amicro-filtration membrane assembly to obtain filtrate containing extracellular polymeric substances, S3, filtering through a multistage membrane assembly, so that the high molecular weight extracellular polymeric substance and the low molecular weight extracellular polymeric substance can be concentrated and purified in sequence, and then the extracellular polymeric substances in different molecular weight ranges are separated out, and S4, conveying the filtrate generated in the step S3 to a forward osmosis membrane assembly taking a fertilizer as a driving agent for filtering to obtain a drawing solution containing the fertilizer, and the drawing solution can be directly used for water and fertilizer integrated irrigation or soilless culture. The method has the characteristics of no celldamage and no pollution.

The object of the present invention is to provide a kind of preparation method of cerebroside carnosine injection with good stability, wherein, the preparation of the rabbit muscle extract that is used to prepare cerebroside carnosine injection comprises the following steps, the rabbit muscle after pretreatment Minced until the volume is not higher than 1cm 3 Then mix the obtained minced muscle with water for injection at a ratio of 1:1-3 (w / w), homogenate, and freeze the obtained muscle homogenate at a temperature not lower than -10°C for 5- After 7 days, put it at 8-10°C to melt for 36-48h. The present invention significantly improves the extraction efficiency of polypeptides in rabbit muscle extracts, effectively avoids the risk of rabbit muscle and its extracts being infected by microorganisms, significantly reduces the freshness of rabbit muscle extracts, and significantly improves the resource utilization rate of rabbit muscles , Drug quality, quality uniformity, effectiveness and safety.