35results about How to "Optimize extraction conditions" patented technology

The invention discloses a method for rapidly measuring a content of bongkrekic acid in food by automatic solid phase extraction-ultra performance liquid chromatography. The method comprises steps of pretreatment of food samples, purification of extracted solution and HPLC analysis to obtain the content of bongkrekic acid in the food samples; and results are proved. The method provided by the invention replaces a traditional manual column passing method with an automatic solid phase extraction instrument to realize automation, reduce labor costs and personal errors, realize controllability of solid phase extraction velocity, improve repeatability and accuracy of experiments and shorten a sample treatment cycle; the method optimizes extraction conditions, chromatographic conditions and the like to obtain an excellent separation degree and chromatographic peak, has advantages of high sensitivity, high recovery rate and relatively short pretreatment cycle, meets requirements on detection of the content of bongkrekic acid in food, provides a reference for a laboratory to carry out relevant detections and plays an active role in ensuring food safety.

The invention relates to a method for extracting total saponins from bark of acanthopanax brachypus harms, and belongs to the field of extraction of active ingredients of natural products. The process flow is that the bark of acanthopanax brachypus harms is taken as raw materials, the mixed solvent of [Bmin]Br and water is taken as extraction solvent, continuous flowing extraction is performed through ultrasonic-microwave collaborative solvent, extraction process is detected through an IR detector, and filter liquor is deposited and centrifuged to obtain clear extracting solution; and the extracting solution is degreased through diethyl ether and is subjected to macroporous absorption resin column chromatography, so that 70% of ethanolic elution is collected, discolored through activated carbon, and impurities are removed through aluminum oxide so as to obtain total saponins. After analysis, the extraction rate achieves more than 8.56%, the content of the total saponins achieves more than 92.53%, and is higher than that of the total saponins obtained by other methods. The method has the advantages of normal pressure extraction, simple process, quickness and simplicity in operation, energy conservation and environmental friendliness and high selectivity, and can perform on-line detection to the extraction process; the saponin is not easy to denature, and does not need to be filtered and separated after extraction; and the extraction rate and purity of the total saponins are high, and the industrialized production can be facilitated.

The invention discloses a method for extracting mitochondrial deoxyribonucleic acid (DNA) of cotton. The method comprises the following steps of: (1) homogenizing cotton, and centrifuging and collecting precipitate; (2) removing nuclear DNA, adding ethylene diamine tetraacetic acid (EDTA) solution, and centrifuging to obtain the precipitate; (3) adding buffer solution, and centrifuging to obtain mitochondria; (4) adding lysis solution and acetate solution, centrifuging, collecting supernatant, and extracting by using chloroform-isoamylol; (5) adding acetate solution and absolute ethanol, centrifuging to obtain the precipitate, and washing by using ethanol; (6) dissolving in triisopropylphenylsulfonyl (Tris)-EDTA (TE); (7) removing ribonucleic acid (RNA) and protein, extracting by using Tris saturated phenol-chloroform-isoamylol, and centrifuging and collecting the supernatant; and (8) adding the acetate solution and the ethanol, and centrifuging to obtain the precipitate; and washing by using the ethanol to obtain the mitochondrial DNA. Aiming at defects of the conventional extraction of the mitochondrial DNA, the conventional cetyltrimethylammonium bromide (CTAB) method is improved, and the extraction conditions are optimized by adjusting the time and times of centrifugation, changing reagents, increasing extraction and washing times, and the like. On the basis of ensuring that the mechanical damage degree of the mitochondria is minimum, the nuclear DNA, phenols and polysaccharides outside the mitochondria are effectively removed, pollution rate is reduced, the purity and the quality of the mitochondrial DNA are improved, and the mitochondrial DNA can meet the requirement of subsequent experiments of the gene engineering of the cotton mitochondria.

The invention relates to an efficient method for extracting lutein from Cordyceps militaris, which comprises the following steps: preparing raw materials, performing fermentation treatment, performing ionic liquid extraction and purifying. By performing optimal combination on the extraction process and creatively adopting an ionic liquid/assistant composite extraction manner, the method achieves the purpose of efficiently extracting lutein from Cordyceps militaris, obtains the technical effect that the extraction rate is far higher than that in the prior art, greatly increases the utilization ratio of the raw materials, and avoids the consumption of a large amount of organic solvent. Thus, the method has the advantages of being green, environment-friendly and suitable for large-scale production, and has an application prospect of being widely industrialized.

The invention discloses a method for extracting and purifying physalis polysaccharides by using ultrasonic waves, and belongs to the field of preparation of extracted polysaccharides. The preparation method comprises the following steps: washing, beating, drying and pulverizing the physalis and passing through a 60-mesh sieve to obtain the physalis powder; after ultrasonic treatment under different conditions, centrifuging, and the supernatant is the polysaccharide extract. Through the single factor test, the optimum ratio of liquid to material, ultrasonic temperature, ultrasonic time, and ultrasonic power for extracting physalis polysaccharides were determined. Volume ratio of sample solution-sevage reagent, volume ratio of chloroform-n-butanol, shaking time, deproteinization times. Hydrogen peroxide decolorization, through a single factor test, finally determined the optimum decolorization temperature, solution pH value, decolorization time, hydrogen peroxide addition amount.

The invention belongs to the technical field of Mongolian medicine, and the method comprises the following steps of: preparing Sug Mel-4 soup; preparing a rutin reference solution; selecting the maximum wavelength; drawing a standard curve; single factor experiment: taking the flavone content as an indicator, and observing the influence of a liquid-to-liquid ratio, an extraction time and an extraction temperature on the flavone content by using a single factor method; response surface method optimization process; and orthogonal method optimization process verification: using time A, temperature B, and material-liquid ratio C as experimental factors to perform an orthogonal design experiment. The extraction conditions are optimized by using the response surface and orthogonal design experiments, the influence of the extraction time, the extraction temperature and the liquid-to-liquid ratio on the flavone content in the Sug Mel-4 soup is observed, the flavone yield in the Sug Mel-4 soupis used as the indicator, the optimal process conditions are that the material-liquid ratio is 1:40, the time is 120min, the temperature is 100 DEG C, it is simple, convenient, feasible, stable and reliable to increase the flavone content in the Sug Mel-4 soup by using a water bath heating reflux method, and the method is suitable for industrial production.

The invention discloses a method for separating and purifying T lymphocytes. The method comprises the following four steps: 1) separating plasma; 2) separating lymphocytes; 3) cleaning lymphocytes; 4)purifying lymphocytes. According to the technical scheme of the invention, extraction conditions of T lymphocytes are optimized and effective utilization of T lymphocytes is benefited.

The invention discloses a method for detecting residues of eight organic matters in ion exchange resin by adopting a headspace gas phase method, which comprises the following steps of: performing ultrasonic extraction on a sample by using dimethyl sulfoxide by using a chemical analysis technical method, optimizing an extraction method and chromatographic conditions of the sample, and performing baseline separation and statistics on the eight residues; and enabling the linear correlation coefficients in the investigated concentration range to be all 0.999 or above, obtaining the lowest detection limit is obtained. The method is simple, accurate and reliable, and is suitable for determining the organic residual organic solvent in the adsorption resin.

The invention relates to the technical field of preparation of beverages, in particular to a method for preparing a cucumber extract. The method comprises the following steps of (1) taking fresh cucumbers, thoroughly cleaning the taken fresh cucumbers, adding water which is 18-26 times of the cucumbers, and performing mashing so as to obtain paste; (2) putting the paste to a magnetic field of which the magnetic field intensity is 3000-3400Gs, and performing magnetisation under the temperature of 45-55 DEG C so as to obtain magnetised paste; (3) performing microwave extraction under the power of 600-700W on the magnetise paste for 30-50s so as to obtain a cucumber extracting solution; (4) uniformly mixing the cucumber extracting solution with honey and table salt in the weight ratio of the cucumber extracting solution to the honey to the table salt being (90-120) to (5-12) to (0.001-0.01); and (5) performing filtering, performing instantaneous sterilizing at ultrahigh temperature, and performing filling so as to obtain the cucumber extract. Through the adoption of the method disclosed by the invention, the efficacy of reducing weight of the cucumber extract can be improved.

The invention discloses an extracting method of aluminum. Basic compounds and fly ash are adopted for forming, and sintering is performed; then a sintered sample block is subjected to acid leaching, and aluminum-containing crude liquid is obtained; the roasting temperature and the temperature and time of acid leaching are greatly lowered; the lowest temperature can reach 50-60 DEG C, and the efficiency of aluminum extraction reaches up to 88%; the SiO2 content in leached slag reaches 95%, and aluminum and silicon separation is achieved; due to agglomeration treatment, a porous structure of a mesoscale is molded for a reaction; the distance between two phases of a solid-liquid ratio is shortened, the mass transfer condition of a gas-liquid two phase is also effectively improved, and therefore extraction of aluminum is reinforced; and fly ash particles and capillary channels in the block sample play a framework role similar with coke in a blast furnace, and the reason that the aluminum oxide extracting rate is high due to an agglomeration means can be explained from the aspect of dynamics.

The present invention relates to the technical field of drink preparations and especially relates to a preparation method of cucumber dews. The preparation method comprises the following steps: 1) fresh cucumbers are taken, the taken cucumbers are washed clean, water at 20-30 times of the weight of the cucumbers is added, and the washed cucumbers are beat into slurry; 2) the slurry is put into a magnetic field at a magnetic field strength of 3000-3400 Gs to conduct magnetization for 10-20 min to obtain a magnetized slurry; 3) then the magnetized slurry is subjected to an ultrasonic extraction for 20-30 min at a power of 400-500 W to obtain an extract; 4) the cucumber extract is subjected to a double filtration, the filtered extract is clarified, the clarified extract is subjected to a centrifugal separation to obtain cucumber raw juice; and 5) the cucumber raw juice is subjected to an ultra-high temperature instantaneous sterilization, and the sterilized mixture is filled to obtain the cucumber dews. The prepared cucumber dews by the method can improve the anti-aging effects of the cucumber dews.

The invention relates to a pollution-free integrated method and device for high-added-value extracellular polymeric substance recovery. The method comprises the following steps: S1, conveying excess sludge into a cation exchange resin reactor, and extracting extracellular polymeric substances in the excess sludge, S2, intercepting microbial cells and residues thereof in the excess sludge through amicro-filtration membrane assembly to obtain filtrate containing extracellular polymeric substances, S3, filtering through a multistage membrane assembly, so that the high molecular weight extracellular polymeric substance and the low molecular weight extracellular polymeric substance can be concentrated and purified in sequence, and then the extracellular polymeric substances in different molecular weight ranges are separated out, and S4, conveying the filtrate generated in the step S3 to a forward osmosis membrane assembly taking a fertilizer as a driving agent for filtering to obtain a drawing solution containing the fertilizer, and the drawing solution can be directly used for water and fertilizer integrated irrigation or soilless culture. The method has the characteristics of no celldamage and no pollution.