35results about How to "Optimize extraction conditions" patented technology

The invention discloses a method for analyzing the content of adenosine and cordycepin in the sporocarp of cordyceps militaris through artificial culture by virtue of high performance liquid chromatography (HPLC). The method comprises the following specific steps: 1, weighing the dried sporocarp powder of cordyceps militaris in a 50ml centrifuge tube with a plug, adding water, ultrasonically extracting the centrifuge tube in water bath, then centrifuging and taking a supernate as a test solution; 2, weighing a certain amount of standard substances of adenosine and cordycepin, adding water and dissolving to prepare a mixed solution as a mixed standard solution; 3, performing content measuring, namely taking the mixed standard solution and test solution respectively, filtering the solutions through a 0.45um aqueous phase millipore filter and injecting the filtered solutions into a small bottle, and measuring the chromatographic peak of the cordyceps militaris sporocarp test solution and the adenosine cordycepin standard solution with the same chromatographic retention time. The invention provides a new, simple and convenient method for evaluating the quality of the cordyceps militaris sporocarp.

The invention relates to an efficient method for extracting lutein from Cordyceps militaris, which comprises the following steps: preparing raw materials, performing fermentation treatment, performing ionic liquid extraction and purifying. By performing optimal combination on the extraction process and creatively adopting an ionic liquid / assistant composite extraction manner, the method achieves the purpose of efficiently extracting lutein from Cordyceps militaris, obtains the technical effect that the extraction rate is far higher than that in the prior art, greatly increases the utilization ratio of the raw materials, and avoids the consumption of a large amount of organic solvent. Thus, the method has the advantages of being green, environment-friendly and suitable for large-scale production, and has an application prospect of being widely industrialized.

The invention provides a method for extracting gastrodin by an enzyme method. The method is characterized by comprising the following steps: dissolving rhizoma gastrodiae powder in acetic acid-sodium acetate buffer solution and mixing the two substances evenly; adding complex enzyme into the solution for stirring evenly and enzymolysis; adding alcohol into the mixture to for sufficiently stirring, filtering and deslagging; concentrating extracting solution into extract by a rotary steaming method; and drying the extract to obtain the gastrodin extract. The method has the advantages that as high efficiency and specificity of biological enzyme and mild acting condition, compared with an ultrasonic extracting process, the extracting condition is more convenient, and the equipment is more simplified, so that energy consumption is reduced, and the cost is saved; compared with a hot alcohol refluence extracting process, the extracting condition is simple, the temperature of an extracting system is reduced, so energy consumption of heating is reduced, the pollution to the environment is reduced, a micromolecular drug effect ingredient with thermal volatilization can be remained, and the drug effect of the obtained gastrodin is more complete; and compared with the prior art, the extracting rate is improved by 0.05 to 0.13 percent.

The invention relates to a pollution-free integrated method and device for recycling extracellular polymers with high added value. Wherein, the method includes the following steps: step S1: transport the excess sludge to a cation exchange resin reactor, and extract extracellular polymers in the excess sludge; step S2: intercept the microbial cells and their components in the excess sludge through the microfiltration membrane module Residue, obtain the filtrate containing extracellular polymers; Step S3: Filtrate through multi-stage membrane modules, which can sequentially concentrate and purify high molecular weight extracellular polymers and low molecular weight extracellular polymers, and then separate different molecular weight range of extracellular polymers; step S4: the filtrate produced by the filtration of step S3 is transported to the forward osmosis membrane module using fertilizer as the driving agent for filtration to obtain a drawing solution containing fertilizer, which can be directly used for Water and fertilizer integrated irrigation or soilless cultivation. The invention has the characteristics of no damage to cells and no pollution.

The invention discloses a method for extracting mitochondrial deoxyribonucleic acid (DNA) of cotton. The method comprises the following steps of: (1) homogenizing cotton, and centrifuging and collecting precipitate; (2) removing nuclear DNA, adding ethylene diamine tetraacetic acid (EDTA) solution, and centrifuging to obtain the precipitate; (3) adding buffer solution, and centrifuging to obtain mitochondria; (4) adding lysis solution and acetate solution, centrifuging, collecting supernatant, and extracting by using chloroform-isoamylol; (5) adding acetate solution and absolute ethanol, centrifuging to obtain the precipitate, and washing by using ethanol; (6) dissolving in triisopropylphenylsulfonyl (Tris)-EDTA (TE); (7) removing ribonucleic acid (RNA) and protein, extracting by using Tris saturated phenol-chloroform-isoamylol, and centrifuging and collecting the supernatant; and (8) adding the acetate solution and the ethanol, and centrifuging to obtain the precipitate; and washing by using the ethanol to obtain the mitochondrial DNA. Aiming at defects of the conventional extraction of the mitochondrial DNA, the conventional cetyltrimethylammonium bromide (CTAB) method is improved, and the extraction conditions are optimized by adjusting the time and times of centrifugation, changing reagents, increasing extraction and washing times, and the like. On the basis of ensuring that the mechanical damage degree of the mitochondria is minimum, the nuclear DNA, phenols and polysaccharides outside the mitochondria are effectively removed, pollution rate is reduced, the purity andthe quality of the mitochondrial DNA are improved, and the mitochondrial DNA can meet the requirement of subsequent experiments of the gene engineering of the cotton mitochondria.

The invention discloses a method for detecting n-propyl bromide in leather and textiles. The method is characterized in that the gas chromatography-mass spectrometry combination is used for detection. The detection method is simple, convenient and fast and has high sensitivity. A sample to be detected is in the linear relationship in the range of 0.1-50mg / L, the correlation coefficient is 0.9995, the method detection limit is 0.1mg / L, the standard recovery rate is 96%-112%, and the relative standard deviation (RSD) (n is equal to 7) is less than 2%. The method can be used for detecting the n-propyl bromide in the leather and textiles very well.

The object of the present invention is to provide a kind of preparation method of cerebroside carnosine injection with good stability, wherein, the preparation of the rabbit muscle extract that is used to prepare cerebroside carnosine injection comprises the following steps, the rabbit muscle after pretreatment Minced until the volume is not higher than 1cm 3 Then mix the obtained minced muscle with water for injection at a ratio of 1:1-3 (w / w), homogenate, and freeze the obtained muscle homogenate at a temperature not lower than -10°C for 5- After 7 days, put it at 8-10°C to melt for 36-48h. The present invention significantly improves the extraction efficiency of polypeptides in rabbit muscle extracts, effectively avoids the risk of rabbit muscle and its extracts being infected by microorganisms, significantly reduces the freshness of rabbit muscle extracts, and significantly improves the resource utilization rate of rabbit muscles , Drug quality, quality uniformity, effectiveness and safety.

The invention discloses a solution and an ultrasonic method for extracting earthworm coelomocytes, belonging to the technical field of bioengineering. Each 3 mL of the solution for extracting the earthworm coelomocytes contains 60-70 mM of NaCl, 5-6% by weight of ethanol, 50-60 mM of guaicol glyceryl ether and 5-6mM of EGTA PBS buffer solution. The operation steps of the ultrasonic method are as follows: step 1, clearing the bowel of an earthworm, namely, using the PBS buffer solution for repeated cleaning; step 2, putting the earthworm into the cold solution for extracting the coelomocytes, and carrying out fast ultrasonic extraction; step 3, removing the earthworm, adding LBBS buffer solution for cleaning, centrifuging and removing supernate, wherein obtained precipitates are the coelomocytes; step 4, adding cell sap for incubation; and step 5, using trypan blue for coloring and microscopic examination, and calculating cell viability. The solution and the ultrasonic method extract a great many coelomocytes, realizes high cell viability, can satisfy the needs of apoptosis and a single cell gel electrophoresis experiment, and can be used for early diagnosis of soil pollution and function for early warning.