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Method for optimizing flavone content in Sug Mel-4 soup

A technology for content and flavonoids, applied in the field of optimizing the content of flavonoids in Sugermule-4 soup, can solve problems such as inaccuracy, and achieve the effect of optimizing extraction conditions and flavonoid content easily and easily.

Inactive Publication Date: 2019-08-13
鄂尔多斯市疾病预防控制中心
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Problems solved by technology

Although one of the material bases of the curative effect of the Mongolian medicine compound is volatile oil, in the traditional decoction process, the volatile oil is not extracted separately for consumption, so it is inaccurate to optimize the extraction process with volatile oil as an index

Method used

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  • Method for optimizing flavone content in Sug Mel-4 soup
  • Method for optimizing flavone content in Sug Mel-4 soup
  • Method for optimizing flavone content in Sug Mel-4 soup

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Embodiment Construction

[0045] The present invention will be further described with reference to the drawings and embodiments.

[0046] In one embodiment, as Figure 1-6 As shown, a method for optimizing flavonoid content in Sugemule-4 soup comprises the steps:

[0047] ⑴ Preparation of Sugemule-4 Soup

[0048] Firstly, the jujube seed, white cardamom, Baijusheng and Pichu medicinal materials were crushed into powder respectively, and 1.5g, 1.5g, 1.0g and 0.5g of the Chinese jujube kernel, white cardamom, Baijusheng and Pichu medicinal powder were weighed and placed in the In the same round bottom flask, add 10, 20, 30, 40 or 50 times of water, shake, mix and soak, put the round bottom flask into the water bath of the water bath heating and reflux device, heat to 100°C, and keep it within 120min At a temperature of 100°C, receive sample solution: Sugemule-4 soup, set aside;

[0049] (2) Preparation of reference solution

[0050]Accurately weigh 10 mg of rutin standard substance with an analytical...

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Abstract

The invention belongs to the technical field of Mongolian medicine, and the method comprises the following steps of: preparing Sug Mel-4 soup; preparing a rutin reference solution; selecting the maximum wavelength; drawing a standard curve; single factor experiment: taking the flavone content as an indicator, and observing the influence of a liquid-to-liquid ratio, an extraction time and an extraction temperature on the flavone content by using a single factor method; response surface method optimization process; and orthogonal method optimization process verification: using time A, temperature B, and material-liquid ratio C as experimental factors to perform an orthogonal design experiment. The extraction conditions are optimized by using the response surface and orthogonal design experiments, the influence of the extraction time, the extraction temperature and the liquid-to-liquid ratio on the flavone content in the Sug Mel-4 soup is observed, the flavone yield in the Sug Mel-4 soupis used as the indicator, the optimal process conditions are that the material-liquid ratio is 1:40, the time is 120min, the temperature is 100 DEG C, it is simple, convenient, feasible, stable and reliable to increase the flavone content in the Sug Mel-4 soup by using a water bath heating reflux method, and the method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of Mongolian medicine, in particular to a method for optimizing the content of flavonoids in Sugemule-4 soup. Background technique [0002] Sugemul-4 Soup is an improved formula based on the traditional recipe Sugemul-3. The traditional formula Sugemule-3 is a commonly used clinical prescription in Mongolian medicine. It is composed of 15g of white cardamom, 10g of Baijusheng and 5g of Pipera three-flavored Chinese herbal medicine. It has been reported in the literature that the extraction process of white cardamom-bachou volatile oil in Sugemule-3 recipe was optimized by using the traditional formula Sugemule-3 as an index to optimize the extraction process of white cardamom-bachovy volatile oil. The optimization of the extraction process can maximize the extraction of active ingredients, which improves the internal quality and clinical treatment effect of the preparation, and maximizes the effect of the M...

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 靳尚武孙利东白东浩查干夫孟根花果佳瀮
Owner 鄂尔多斯市疾病预防控制中心
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