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A kind of cerebroside carnosine injection with good stability and preparation method and application thereof

A technology of cerebroside muscle and injection, which is applied in the field of medicine, can solve the problem of affecting the quality of drugs, quality uniformity and quality control effectiveness and safety, and increase the risk of microbial infection of rabbit muscle and its extract Rabbit muscle extract Freshness, affecting the extraction efficiency of peptide products and other issues, to achieve the effect of improving peptide extraction efficiency, improving stability and safety, and improving resource utilization

Active Publication Date: 2022-02-11
BEIJING SIHUAN PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing rabbit muscle extract preparation method has the following defects. One is that the rabbit muscle needs to be repeatedly frozen and thawed to destroy the cell membrane and effectively extract the protein polypeptide product in the cell membrane. The freezing and thawing temperature and time of the rabbit muscle affect the polypeptide product. The second is to repeatedly thaw rabbit muscle at room temperature or higher than room temperature, which increases the risk of microbial infection of rabbit muscle and its extract and the freshness of rabbit muscle extract, which affects the quality and quality uniformity of the drug and quality control, efficacy and safety

Method used

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  • A kind of cerebroside carnosine injection with good stability and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 The present invention is used to prepare the rabbit muscle extract of cerebroside and carnosine injection

[0055] The present invention is used for the preparation of the rabbit muscle extract of cerebroside carnosine injection and comprises the following steps:

[0056] (1) Take 10kg of fresh rabbit muscle, remove the fat and connective tissue, wash it, and mince it until the volume is not higher than 1cm 3 Then add the obtained minced muscle into 10L of water for injection, mix evenly, homogenate, and then freeze the obtained muscle homogenate at not lower than -10°C for 7 days, then place it in 8 Thaw at ℃ for 36 hours, repeat the freezing and thawing treatment of the rabbit muscle homogenate twice according to the aforementioned freezing and thawing conditions, heat the obtained rabbit muscle homogenate to 60°C and keep it warm for 20 minutes, then cool it down to room temperature, Centrifuge at 4000 rpm for 20 minutes to obtain a filtrate;

[0057] (2...

Embodiment 2

[0060] Example 2 The present invention is used to prepare the rabbit muscle extract of cerebroside and carnosine injection

[0061] The present invention is used for the preparation of the rabbit muscle extract of cerebroside carnosine injection and comprises the following steps:

[0062] (1) Take 10kg of fresh rabbit muscle, grind it after pretreatment, and grind it to a volume no higher than 1cm 3 Then add 10L of water for injection to the obtained minced muscle, mix evenly, homogenate, place the obtained muscle homogenate at no lower than -10°C for 7 days, and then place it at 10°C After thawing at low temperature for 48 hours, the rabbit muscle homogenate was repeatedly frozen and thawed twice according to the aforementioned freezing and thawing conditions, and the obtained rabbit muscle homogenate was heated to 60°C, kept for 20 minutes, and then cooled to room temperature. Centrifuge at 4000 rpm for 20 minutes to obtain a primary filtrate;

[0063] (2) Adjust the pH ...

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Abstract

The object of the present invention is to provide a kind of preparation method of cerebroside carnosine injection with good stability, wherein, the preparation of the rabbit muscle extract that is used to prepare cerebroside carnosine injection comprises the following steps, the rabbit muscle after pretreatment Minced until the volume is not higher than 1cm 3 Then mix the obtained minced muscle with water for injection at a ratio of 1:1-3 (w / w), homogenate, and freeze the obtained muscle homogenate at a temperature not lower than -10°C for 5- After 7 days, put it at 8-10°C to melt for 36-48h. The present invention significantly improves the extraction efficiency of polypeptides in rabbit muscle extracts, effectively avoids the risk of rabbit muscle and its extracts being infected by microorganisms, significantly reduces the freshness of rabbit muscle extracts, and significantly improves the resource utilization rate of rabbit muscles , Drug quality, quality uniformity, effectiveness and safety.

Description

technical field [0001] The invention relates to the field of medicine, and relates to a stable cerebroside and carnosine injection, a preparation method thereof and an application thereof. Background technique [0002] Cerebroside carnosine injection can penetrate the blood-brain barrier, and has the functions of repairing, regenerating, nourishing and protecting nerve tissue and peripheral nerves. It is clinically used in the treatment of stroke, senile dementia, neonatal hypoxic-ischemic encephalopathy, cerebral Brain injury, spinal cord injury, and central nervous system injury caused by other reasons, as well as peripheral nerve injury such as traumatic peripheral nerve injury, diabetic peripheral neuropathy, and compressive neuropathy. The active ingredients of cerebroside and carnosine injection are composed of rabbit muscle extract and bovine brain ganglioside extract. The main active ingredients include polypeptides, various gangliosides, free amino acids, nucleic ac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/01A61K35/34A61K9/08C07K1/34C12P21/06A61P25/28A61P9/10A61P25/00A61P25/02A61P3/10
CPCA61K38/012A61K35/34A61K9/0019A61K9/08C12P21/06C07K1/34A61P25/28A61P9/10A61P25/00A61P25/02A61P3/10
Inventor 王建辉王洪敏宋春风王丽媛杨玉辉赵月赵友明宋春蕾
Owner BEIJING SIHUAN PHARMA
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