63 results about "Cerebroside" patented technology

A recombinant fungal host cell producing recombinant glucocerebrosidase is provided. A functional recombinant glucocerebrosidase produced in recombinant fungal host cells is also provided. Methods for producing and isolating functional recombinant glucocerebrosidase from fungal hosts are also provided.

The invention relates to a medicament for nerve regeneration promotion, and particularly to a nerve regeneration promotion injection and a preparation method thereof. The nerve regeneration promotion injection is composed of the following ingredients in proportion: 280-320 micrograms of mouse nerve growth factor for injection, 19-21ml of single sialic acid four hexose ganglioside sodium injecta, 18-22ml of cerebroside-kinin injecta, 9-11ml of mecobalamine injecta and 9-11ml of dexamethasone acetate injecta. The five effective ingredients of the medicament provided by the invention are combined together and cooperative actions of multiple nutritive factors are utilized, so that the medicament has a significant curative effect on repairing of injured nerve, especially the repairing of spinal nerve injury. The medicament provided by the invention also has a protection effect on spinal cord transected injury at the early stage.

The invention discloses a method for separating cerebroside from sea cucumber by a high-speed countercurrent chromatography, which is characterized by comprising the following steps of: adding mixed solution of chloroform and methanol into sea cucumber dry powder, soaking at room temperature, and concentrating filter liquor to obtain a cerebroside crude extract; mixing petroleum ether, methanol and water, and respectively performing ultrasonic degasification on the formed stationary phase and mobile phase; dissolving the crude extract by using the mixed solvent, and filtering to obtain samplesolution; and pumping the stationary phase into a chromatographic column of the high-speed countercurrent chromatography, adjusting the rotating speed of a main machine and the flow rate of the mobile phase, pumping in the mobile phase, after two phase equilibria, pumping the sample solution into the chromatographic column through an introduction valve, continuously pumping in the mobile phase, collecting by steps, detecting by using a thin layer chromatography, merging the target object, concentrating, and freeze-drying to obtain the cerebroside. The preparation process has the advantages ofsimpleness, simple and convenient operation, high purification efficiency, short separation time, high stability, and suitability for separation of the cerebroside from various sea cucumber samples and industrial production.

The invention provides a moisturizing mask and a preparation method thereof. The moisturizing mask comprises the following components, by weight: 0.01 to 1% of saccharide isomeride, 0.01%-1% of rhizobium gum, 0.01%-1% of tremella extract, 0.01%-0.2% of sodium hyaluronate, 0.01%-1% of codium tomentosum extract, 0.04%-1.5% of a skin conditioner, 0.1%-0.3% of a pH regulator, 0.06%-0.45% of a thickener and the balance being water. According to the moisturizing mask, various moisturizing active matters are mixed based on a scientific proportion; gene expression of filaggrin, loricrin, hyaluronan synthase-3 and acid sphingomyelinase in the skin can be up-regulated and the water locking capacity of the skin can be improved; meanwhile, synthesis of ceramide and cerebroside is promoted, absorptionof the skin to surrounding moisture is improved, so that the purpose of efficient moisturizing is achieved.

The invention discloses a separation and purification method for cerebroside and a ceramide type compound. The method comprises the following steps of: extracting soya bean lecithin serving as a raw material with petroleum ether with stirring and centrifuging the mixture; extracting lower-layer rough cerebroside solid with a low-polarity organic solvent; extracting the extracted insoluble substance with hot ethanol; combining the extract and concentrating and evaporating the combined extract to dryness to obtain purified rough cerebroside; separating the rough cerebroside by silica gel columnchromatography to obtain a dry substance I; and separating the dry substance by secondary silica gel column chromatography to obtain pure cerebroside and pure ceramide. The separation and purification method has the advantages of high raw material utilization ratio, simple process, low production cost, high purity of the obtained product, high yield and good industrial prospect.

The invention discloses a processing method of a salmon and relates to the field of food processing. The processing method comprises the steps of: killing a salmon, cleaning, and standing in an environment at 30-40 DEG C; then placing the salmon on cotton yarns, and processing for 80 seconds in the environment with the vacuum degree of 5MPa; then preserving for 24 hours by using salt; cleaning, and then baking to complete the processing of the salmon. According to the processing method, the salmon is processed at low temperature, a large amount of salmon cerebroside is extracted by virtue of a vacuum technology, and the salmon is finally processed through preserving and baking methods, so that the processed salmon can be cooked, air-dried and processed while keeping the integrity and quality of the salmon, and the related technological gap in the field is filled.

The invention provides application of cerebrosides in preparation of analgesic drugs. An animal experiment on pharmacology verifies that the cerebrosides are administrated through the ways such as intraperitoneal injection, gavage and skin smearing; all the three ways can improve the threshold values of a mouse to pains caused by heat and mechanical stimulation in a dose-dependent manner; the cerebrosides can remarkably relieve a neuropathic pain, an inflammatory pain and an acetic-acid-induced visceral pain. A cerebroside A or a cerebroside B can inhibit the activity of a vanilloid receptor TRPV1, so that the cerebrosides can increase an algesia threshold value and achieve the purpose of abirritation by lowering the activity of the vanilloid receptor TRPV1. According to the invention, the application of the cerebrosides serving as internal and external analgesic drugs is developed.

The invention discloses the application of cerebroside B which is extracted and prepared from termitomyces in preparing a medicine for treating ischemic brain injury. The application proves that a cerebroside compound has obvious treatment function on ischemic brain injury through pharmacology experiments, can reduce the cerebral infarction area which is caused by cerebral ischemia and relieve the cerebral edema through a blood-brain barrier, reduce the death of nerve cells, and simultaneously promote recovery of movement and cognitive functions after cerebral apoplexy, so the death rate of the cerebral apoplexy is reduced. The application develops the new medicine application of the cerebroside compound, and new therapeutic drugs are provided for treating diseases of the cerebral apoplexy.

Provided, among other things, is a delivery composition comprising: an aqueous carrier; a lipid component suspended in the carrier comprising significant amounts of type A lipid, fatty acid, and bilayer-stabilizing steroid(s), wherein type A lipid is one or more of any of phospholipid, ceramide(s) sphingomyelin(s) and glucocerebroside(s); and a bioactive agent, wherein (a) the delivery composition is packaged with a label with directions for mucosal, pulmonary or oral administration, and/or (b)(i) the viscosity of the composition is adjusted to a viscosity appropriate for spraying and/or (ii) the type A lipid comprises conjugate(s) of lipid-phase anchoring hydrophobic moieties and flexible, soluble polymers, and/or (iii) comprises a stabilizing effective amount of soluble polymers.

The present invention relates to a process for purifying recombinant human Galactocerebroside β-Galactosidase dase (rhGALC) from a cell culture, wherein a fraction of said cell culture comprising rhGALC is subjected to chromatography on three distinct resins.

A method of treating Gaucher's disease in a subject in need thereof is provided. The method comprising orally administering to the subject a therapeutically effective amount of recombinant glucocerecbrosidase (GCD) comprised in plant cells, wherein said therapeutically effective amount of GCD corresponds to 1-1920 units/Kg/14 days, thereby treating Gaucher's disease. Also provide unit dosage forms which comprise the glucocerecbrosidase (GCD) comprised in plant cells.

Disclosed are LIMP-2 peptides, LIMP-2 polypeptides, variants thereof, and pharmaceutical compositions comprising the LIMP-2 peptides, LIMP-2 polypeptides, or variants thereof. The disclosed peptides and polypeptides preferably comprise an amino acid sequence that is sufficient for providing a biological activity associated with LIMP-2, which may include binding and/or activating biological molecules such as β-glucocerebrosidase and binding viral protein 1 (VP1) of enterovirus 71 (E71) or coxsackievirus A16 (CA16). Also disclosed are methods of using the LIMP-2 peptides, LIMP-2 polypeptides, and variants thereof as therapeutics for treating diseases and disorders associated with β-glucocerebrosidase activity in subjects in need thereof.

The invention belongs to the technical fields of separation and purification of natural products, and discloses a method for extracting cerebroside from gynura procumbens. According to the method provided by the invention, the gynura procumbens is used as a raw material, and through the steps of two-phase solvent mixed extraction, column chromatography, crystallization and the like, the novel cerebroside can be quickly separated from the gynura procumbens; the product obtained by the method has high purity, natural molecules are not destroyed, and the product is suitable for large-scale production; and the obtained cerebroside has excellent anti-inflammatory activity and can be used for developing related anti-inflammatory drugs.

Disclosed are new small molecules having a pyrrolopyrimidine core structure and the uses thereof for modulating glucocerebrosidase activity. Also disclosed are pharmaceutical compositions comprising the small molecules which may be administered in methods of treating diseases or disorders associated with glucocerebrosidase activity, includin neurological diseases and disorders such as Gaucher's disease and Parkinson's disease. The small molecules may be utilized to generate activated glucocerebrosidase. The activated glucocerebrosidase thusly generated can be administered in enzyme replacement therapy and/or utilized in screening assays for new small molecules that bind to the activated glucocerebrosidase and/or modulate the activity of the activated glucocerebrosidase.

The invention relates to a new application of holothurian cerebroside and a derivatives thereof, in particular to an application of holothurian cerebroside and derivatives thereof in products for improving blood-brain barrier injury. Experiments prove that the holothurian cerebroside remarkably reduces blood-brain barrier injury caused by ischemia reperfusion and blood-brain barrier injury causedby lead acetate, the effect of the holothurian cerebroside and derivatives thereof in improvement of the blood-brain barrier is verified for the first time, and a new way is provided for treatment ofthe blood-brain barrier.

The present invention discloses a dry pretreatment and preparation method for improving extraction rate of cerebroside in lentinus edodes stems, and belongs to the field of food by-product processing.The method comprises the following steps: S1, pre-cooling fresh lentinus edodes stems; S2, drying the lentinus edodes stems using microwave/hot air until moisture content of the lentinus edodes stemsis less than 10%; S3, pulverizing and sieving the dried lentinus edodes stems to obtain lentinus edodes stem powder; S4, adding an extraction solution A into the lentinus edodes stem powder accordingto a material-liquid ratio A, using microwave/ultrasonic cooperative treatment, and then performing a centrifugation treatment A, pouring a supernatant A after the treatment and taking a precipitatefor a standby application; and S5, adding an extraction solution B to the precipitate according to a material-liquid ratio B, using an ultrasound treatment, then conducting a centrifugation treatmentB, taking out an obtained supernatant B after the treatment, and preparing a cerebroside-enriched sample. The established drying and extracting method for highly efficiently extracting cerebroside from lentinus edodes stems is short in time-consuming and high in extraction rate.

The invention discloses a method for determining faeces brain thioglycoside based on flight mass spectrometry, and relates to the technical field of mass spectrometry detection. The method comprises the following steps: S1, taking 1g of excrement, adding the excrement into a mixed solution composed of normal hexane and isopropanol, and separating and extracting thiocerebroside; s2, evaporating the purified sulfur cerebroside to dryness, adding 300 microliters of 0.1 N sodium hydroxide, and saponifying in a constant temperature box for 10 minutes to obtain hydrogenated sulfur cerebroside; s3, eluting other lipids by using a high-precision single-head filtering device, and purifying hydrogenated sulfur cerebroside; s4, dissolving the hydrogenated sulfur cerebroside purified in the high-precision single-head filtering device by using methanol, and adding a fixed amount of hydroxylated sulfur cerebroside; s5, completing the analysis of the lipid and the quantity of the hydrogenated sulfur cerebroside by using a matrix-assisted laser desorption ionization time-of-flight mass spectrometer; and S6, completing. According to the method disclosed by the invention, non-invasive determination can be realized in a mode of changing sampling into blood collection into excrement for determination, and the safety and the adaptability are enhanced.