250 results about "Phosphatidylserines" patented technology

A lipid preparation including a glycerophospholipid or salt, conjugate and derivatives thereof, particularly phosphatidylserine (PS), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidyl-inositol (PI), phosphatidylglycerol (PG) and phosphatidic acid (PA), and poly-unsaturated fatty acid (PUFA) acyl groups, particularly long-chain poly-unsaturated fatty acid (LC-PUFA) acyl groups such as omega-3 and / or omega-6 acyl groups, wherein said PUFA is covalently bound to said glycerophospholipid. The preparation possesses an improved bioactivity, and is useful in the treatment of various cognitive and mental conditions and disorders and for maintenance of normal functions of brain-related systems and processes.

Disclosed are new phosphatidylserine binding constructs with surprising combinations of properties, and a range of diagnostic and therapeutic conjugates thereof. The new constructs effectively bind phosphatidylserine targets in disease and enhance their destruction, and can also specifically deliver attached imaging or therapeutic agents to the disease site. Also disclosed are methods of using the new construct compositions, therapeutic conjugates and combinations thereof in tumor vasculature targeting, cancer diagnosis and treatment, and for treating viral infections and other diseases.

Compositions and methods for treating subjects with disorders characterized by hyper-proliferating cells such as tumors and cancers are provided. The compositions comprise agents that are combinations of saposin C (or prosaposin-related polypeptides) and dioleoylphosphatidylserine (or inner leaflet components). This anti-tumor agent is administered in the methods of the invention according to a dosing regimen. Administering an agent of the invention results in a positive therapeutic response in a subject with a tumor.

The invention discloses child development formula milk powder. DHA, phosphatidylserine and choline are combined to facilitate child brain development, lutein is added for protecting child eyesight, the components are reasonably combined with substances such as raw milk, various vitamins, mineral substances and dietary fiber (fructo-oligose and galactooligosaccharide) to give play to the synergistic effect, the effect of promoting comprehensive development of the brain, eyesight and the whole body of children of 3-7 years old is achieved, and the milk powder is good in taste and comprehensive in nutrient. The invention further provides a preparation method of the child development formula milk powder. The child development formula milk powder is obtained through the steps of raw milk inspection and acceptance, milk purification, pre-pasteurization, dosing, preheating, homogenizing, sterilization, condensation, spray drying, cooling and the like in sequence, the steps are simple, the process is easy to control, the nutritional ingredient losses are small, the method is applicable to preparing child formula milk powder, and the obtained milk powder is applicable to children of 3-7 years old.

Modified annexin proteins, including a homodimer of human annexin V, are provided. Methods for their use, such as to prevent thrombosis without increasing hemorrhage and to attenuate ischemia-reperfusion injury (IPI), are also provided. The modified annexins bind phosphatidylserine (PS) on cell surfaces, thereby preventing the assembly of the prothromkinase complex. The modified annexin decreases the binding of leukocytes and platelets during post-ischemic reperfusion, thereby restoring microvascular blood flow and decreasing organ damage.

The invention relates to a stable preparation of phosphatidylserine and a preparation method, application as well as an application product of the stable preparation. The preparation method comprises the following step of processing the phosphatidylserine through microencapsulation, micro-pelletization or liposome encapsulation. The stable preparation of the phosphatidylserine can be independently applied as a medicine or can be added into daily foods, drinks and health-care products, also has an important effect on health care of brains while guaranteeing the color and the taste of the daily foods and the drinks of people and is of great benefit to brain disorder such as nutritional supplement of brain development of students, overstrain of nerves of the adults, and memory loss, brain atrophy and senile dementia of the middle aged and elderly people.

The invention relates to a method for preparing phosphatidylserine and phosphatidylcholine, and belongs to the field of food health-care products. The method comprises the following steps of: extracting soybean meal phospholipid which is used as a raw material by an organic solvent, and then distilling and drying to respectively obtain a phosphatidylcholine crude product and a phosphatidylserine crude product; and separating and purifying the phosphatidylcholine crude product and the phosphatidylserine crude product through a silica gel column of 200-300 meshes, eluting with a mixed organic solvent, concentrating the eluant, and drying to obtain phosphatidylserine and phosphatidylcholine. According to the method, the purities of the phosphatidylserine and the phosphatidylcholine meet requirements of new resource foods, so that the phosphatidylserine and the phosphatidylcholine can be used as new resource dietary supplements and have high economic added values; and the phosphatidylserine and the phosphatidylcholine are prepared at the same time by effectively combining simple extraction of the phosphatidylserine and the simple extraction of the phosphatidylcholine. By the method, the production time is shortened, the production cost is reduced, the production rate is improved, the operation is simple, and the products have safety.

Mixtures of natural phosphatidylcholine species, natural lysophosphatidylcholine species, phosphatidylserine species, phosphatidylethanolamine species, 1-hydroxy-2-acyl-phosphatidylcholine species, 1-hydroxy-2-acyl-phosphatidylserine molecular species, 1-hydroxy-2-acyl-phosphatidylethanolamine molecular species, 1-O-alkyl-2-hydroxy phosphatidylcholine species, 1-O-alkyl-2-docosaheaxnoyl phosphatidylcholine species 1-O-alkyl-2-docosahexaenoyl phosphatidylserine species, and 1-O-alkyl-2-docosahexaenoyl phosphatidylethanolamine species, Methods using the above disclosed mixtures in mammals to treat various conditions.

The bionic composite 3D bone tissue engineering rack material has pores of 200-500 microns and porosity over 80 %, and contains bioactive nanometer CaO-P2O5-SiO2 glass powder 50-80 wt%, type-I collagen 10-35 wt%, hyaluronic acid 2-8 wt% and phosphatidylserine 2-8 wt%. Its preparation process includes mixing the above said components homogeneously, adding buffering agent and cross-linking agent for cross-linking treatment, freezing, freeze drying to dewater completely, soaking in Na2HPO4 solution, washing, dewatering and vacuum drying. The material is used as bone substituting material for repairing damaged bone tissue in medical field.

The present invention relates to a preparation suitable for the prevention and / or treatment of vascular disorders, comprising the following fractions:fraction a) consisting of long chain polyunsaturated fatty acids;fraction b) consisting of phospholipids, which fraction contains at least two different phospholipids selected from the group consisting of phosphatidylserine, phosphatidylinositol, phosphatidylcholine and phosphatidylethanolamine.fraction c) consisting of compounds which are a factor in methionine metabolism, which fraction contains at least one member selected from the group consisting of folic acid, vitamin B12, vitamin B6, magnesium and zinc.

The invention discloses a health care food used for improving memory and a preparation method therefor. The health care food comprises a first component and a second component. The first component is one or more selected from phosphatidylserine PS, ginkgo leaves, cistanche, docosahexaenoic acid powder and acer truncatum seed oil powder. The second component is auxiliary materials. The components are weighed and sieved with an eighty-mesh sieve and are mixed uniformly, the mixture is subjected to tabletting or loading, and a health care food is obtained. Through the above mode, a new resource food and traditional Chinese medicines are combined, the effects of improving memory in synergism are achieved, and the health care food is a safe and effective health care food with a reasonable formula, with a good taste and improving memory.

The invention discloses a recombinant escherichia coli for producing phospholipase D and an application thereof, which belongs to the technical field of bioengineering. A phospholipase D gene is obtained through clone in Streptomyces ambofaciens by means of molecular biology measures, constructed expression plasmid is guided into E. coli BL21(DE3), and phosphatase-containing engineering bacteria formed by highly copying and recombining an expression carrier are obtained through kanamycin resistant plate screening. The recombinant phospholipase D is used for converting phosphatidylcholine and serine to generate phosphatidylserine, and efficient production of vitamin C-2-phosphate is achieved. By carrying out reaction for 2 h at the temperature of 40 DEG C with the pH value of 4.5, the yield of phosphatidylserine can reach 15.8 g / L, the conversion rate is 63.9%, and the space time yield is 7.9 g / L / h.

The invention aims at providing a health product for preventing and treating senile dementia. The health product comprises the following components in parts by weight: 3-7 parts of fructus alpiniae oxyphyllae powder, 8-12 parts of lumbricus protein, 6-14 parts of ginkgo biloba extract, 8-12 parts of lucid ganoderma extract, 3-7 parts of cortex cinnamomi extract, 3-7 parts of phosphatidylserine, 12-18 parts of gamma-aminobutyric acid, 3-7 parts of L-tryptophan, 1-3 parts of niacin, 3-7 parts of vitamin C, 2-6 parts of vitamin E, 0.3-0.7 part of vitamin B6, 0.1-0.4 part of vitamin B12 and 0.005-0.015 part of folic acid. The dosage form of the finally formed drug can be powder, tablet or capsule. The health product for preventing and treating senile dementia is prepared from pure plants or extracts, so that no toxic and side effects are caused; and the health product for preventing and treating senile dementia is simple in prescription, low in cost and good in effect.

The invention discloses a gene sequence for coding phospholipase D, which belongs to the fields of biotechnology and preparation of phospholipids products. The gene sequence of the phospholipase D is sourced from Streptomyces sp.(strain PMF), and the gene sequence capable of being expressed in escherichia coli is obtained by virtue of the optimization of a codon. The invention also constructs a genetically engineered bacterium for producing phospholipase D, the phospholipase D produced by utilizing the recombinant Escherichia coli is used as a catalyst to produce phosphatidylserine, the enzyme is convenient in source, the yield is high, the fermentation process is simple, the cost is low, and the industrialization prospect is good.

Beneficial topical compositions for treating scar or aging skin are provided. In one embodiment, the compositions include Bulbine frutescens, Centella asiatica and a phenol derived from olives, such as oleuropein. The composition may be used in the treatment of scars formed during surgery. The composition may also be used cosmeceutically in the treatment of aged skin, and may include phosphatidylserine, vitamins, and other beneficial anti-aging ingredients.

The invention discloses a method for preparing phosphatidylserine. According to the method, phospholipid and L-serine are subjected to phospholipid translating acylation reaction under the action of phospholipase D to generate the phosphatidylserine, wherein the phospholipid translating acylation reaction is carried out in a homogeneous medium system and the yield of the phosphatidylserine reaches 80-98 percent. The method disclosed by the invention has the advantages that due to the adoption of a green reaction medium, a reaction system is a homogeneous medium system. No phosphatidic acid as a byproduct is generated, high catalytic efficiency of enzyme and favorable quality of products are obtained and the process is safe and environment-friendly.

The invention relates to a composition with an auxiliary memory improvement function. The composition is prepared from the following components in parts by weight: 40-60 parts of DHA spirulina oil, 30-50 parts of phosphatidylserine, 10-20 parts of safflower oil and 5-8 parts of astaxanthin oil. The composition has the technical advantage that the compatibility theory of the compound reflects the latest research results of active components and brain nutrients of the modern research; various unsaturated fatty acids of the brain nutrients and antioxidant ingredients of bioactive factors are reasonably combined; the defects of unilateral memory improvement products are overcome; overall regulation is strengthened from the basis conservation of brain cells; and a healthy brain is rebuilt.

A medicament for treating skin is described. It comprises essentially of bulbine frutescens, centella asiatica and a phenol derived from olive oil preferably oleuropein. The medicament may be used in the treatment of scars formed during surgery. It may also be used cosmeceutically in the treatment of aged skin where it may be used with phosphatidylserine. The medicament can also act in conjunction with anti-acne treatment to diminish scarring from the acne condition.

The invention relates to a composition having effects of relieving anxiety and improving sleep. The composition comprises the following components in parts by weight: 100-200 parts of gamma-aminobutyric acid, 50-100 parts of phosphotidyl serene, 100-200 parts of a lily bulb extract, 100-200 parts of a spina date seed extract and 0.01-0.1 part of vitamin B. Experiment proves that when volunteers taking the composition provided by the invention, the sleep quality is obviously improved (situations of being impatient with anxiety, insomnia, dreaminess, and sleep disorders are obviously alleviated), and the effective rate can achieve 80% or above.

The invention provides an emulsion composition containing lecithin, DHA, other fat-soluble substances and purified water. The content of the purified water in the emulsion composition is more than 70% by weight, the weight ratio of the lecithin to the DHA is 1: (0.005-1), the particle size of fat balls in the emulsion is less than 5 mu m, and the other fat-soluble substances comprise one or more of the following components: arachidonic acid, linolenic acid, alpha-linolenic acid, gamma-linolenic acid, linoleic acid, conjugated linoleic acid, conjugated linoleic acid glyceride, phosphatidylserine, polyene phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl inositol, phosphatidyl glycerol, phytosterol, plant sterol ester, plant stanol ester and medium and long-chain fatty acid. The invention further provides a preparation method of the emulsion composition and application of the emulsion composition in the functional aspects of supplementing phospholipids and unsaturated fatty acids by oral administration, protecting heart and brain blood vessels, reducing blood lipids, reducing blood pressure, treating fatty liver and the like.

This invention provides a method for inducing immune tolerance toward an antigen comprising the antigen in lipidic particles or lipidic compositions. The lipidic particles are made up of phosphatidylserine and phosphatidylcholine, or phosphatidylinositol and phosphatidylcholine. The lipidic compositions comprise the antigen and O-phospho-L-serine. Administration of these composition results in inducing immune tolerance to the antigen.

The invention discloses DHA phosphatidylserine tablets. The DHA phosphatidylserine tablets comprise sorbitol, microcrystalline cellulose, phosphatidylserine, DHA algae oil powder, taurine, spina dateseed powder, grape powder, acerola cherry fruit powder, lily root flour and magnesium stearate. According to the tablets disclosed by the invention, the value of DHA algae oil can be exerted to the largest extent, and the effects of beneficial components in other raw materials can be fully exerted. According to the tablets disclosed by the invention, the phosphatidylserine and DHA algae oil complement each other, so that the tablets can achieve the effect of retarding intelligence weariness and asthenopia and are particularly suitable for modern brainworkers. In addition, the taurine can improve nerve conduction functions and vision functions, and has important effects on maintaining the normal structure and the functions of the eye tissue.

The invention relates to phospholipase D having an amino acid sequence shown as SEQ ID NO.1. The invention further relates to a gene sequence coding the phospholipase D. The nucleotide sequence of thegene is shown as SEQ ID NO.2. The invention provides a method for improving the expression level of the phospholipase D by virtue of a system modification expression component. The method comprises the following steps: screening and replacing signal peptides, ribosome binding sites and promoters, and transforming the constructed recombinant plasmid into host bacteria, wherein the recombination strain is capable of successfully expressing the phospholipase D. The phospholipase D disclosed by the invention has excellent phosphatidyl transformation ability, and the product phosphatidylserine canbe synthesized by taking lecithin and L-serine as the substrate. The recombinant bacteria have excellent enzyme activity stability, the fermentation cycle is short, and a foundation is laid for large-scale industrial production.

The invention discloses child formula milk powder capable of prompting calcium absorption and growth, and belongs to the technical field of dairy products. The formula milk powder contains skimmed milk powder, desalted whey powder, vegetable oil, 1, 3-dioleoyl-2-palmitic acid triglyceride, high fructose corn syrup, lactose, compound vitamins, compound minerals, choline chloride, taurine, fructo-oligosaccharide, docosahexaenoic acid powder, bovine colostrum powder, milk mineral salt, spina date seed powder, hydrolyzed yolk powder, phosphatidylserine, lactoferrin powder, bifidobacterium animalisBb-12 freeze-dried powder and xanthophyll. The formula milk powder for children has the effects of promoting calcium absorption of children, promoting growth of children and benefiting brain and vision development. The formula milk powder is simple in preparation process and suitable for large-scale production.

A phospholipid derivative useful for the preparation of liposomes for efficient uptake of an antitumor agent or a gene intracellularly by a target tumor cell, which comprises a residue of an alcohol compound and a residue of a phospholipid, and comprising a peptide between the residue of an alcohol compound and the residue of a phospholipid, and wherein (a) the alcohol compound is an alcohol compound selected from poly(alkylene glycols) and the like, (b) the phospholipid is a phospholipid selected from phosphatidylethanolamines, phosphatidylcholines, phosphatidylserines and the like, and (c) the peptide is a peptide comprising a substrate peptide that can serve as a substrate of a matrix metalloproteinase, provided that one amino acid residue or an oligopeptide containing 2 to 8 amino acid residues may bind to one or both ends of the substrate peptide.

The invention relates to the technical field of health food, and in particular to an algae oil soft capsule and a preparation method thereof. The algae oil soft capsule is prepared from a content and a capsule wall material, and the content is prepared from the following raw materials in parts by weight: 30 to 50 parts of algae oil, 6 to 20 parts of linolenic acid, 10 to 20 parts of amino acid, 2 to 10 parts of lecithin, 2 to 10 parts of cephalin and 2 to 8 parts of vitamin E, wherein the amino acid is at least one of phosphatidylserine, asparaginic acid, lysine, tyrosine and glutamic acid; main additives of the capsule wall material are prepared from gelatin, glycerinum and water. The algae oil soft capsule provided by the invention is rich in various beneficial ingredients for the growth and development of the brain, capable of remarkably enhancing the memory ability and improving intelligence, simple in preparation technology, convenient to carry, accurate in dosage, and safe and reliable.

The invention relates to a high-performance liquid chromatography of phosphatidylserine. The High-performance liquid chromatography comprises the steps of (1) preparing a phosphatidylserine standard solution, and using a chloroform-methanol solution to dissolve and fix the constant volume to be 80 to 400ug / ml; (2) preparing a phosphatidylserine sample solution, using the chloroform-methanol solution to dissolve and fix the volume, and diluting and preparing the sample solution of which the phosphatidylserine content is within the concentration range of standard serial solutions; (3) measuring the standard solution and the sample solution under the condition that the high-performance liquid chromatography cooperates with an evaporative light-scattering detector, performing logarithmetics on the peak area of the standard solution as a y-coordinate, performing logarithmetics on the concentration of the standard solution as an x-coordinate, drawing a double logarithmic standard curve, and finding the concentration of the sample solution from the double logarithmic standard curve according to the peak area. The high-performance liquid chromatography has the following beneficial effects: due to the fact that the high-performance liquid chromatography cooperates with the evaporative light-scattering detector, a simple and effective method is provided for the quality control during a phosphatidylserine production process through the entering of different mobile phase volume ratios.

The invention provides novel methods for isolating, characterizing, comparing, and using biological components that are present in the cerebrospinal fluid. Such biological structures, called CS-MPs, can be used for identifying biomarkers that reflect the status (or anticipate the development) of disorders of the Central nervous System (CNS). The novel methods, biological products, and related kits make possible the use of CS-MPs and of their components as biomarkers for the diagnosis, prognosis, or monitoring of CNS disorders. The CS-MPs have a diameter comprised between 100 and 1000 nm and contain phosphatidylserine (PS).

The invention relates to actinomycete for production of phospholipase D and application thereof and belongs to the field of biotechnology. The phospholipase D produced by the actinomycetes after fermentation has catalytic action on soybean phosphatidylcholine and L-serine to efficiently produce phosphatidylserine through transesterification. The actinomycete is collected from a wellhead NO.1066 in Dongfeng town, Dawa County, Panjin city, Liaoning province, and is identified as Streptomycesbottropensis, preserved in the key laboratory for screening of new drugs in Liaoning province under the number of SYP-A-6632, and is preserved in CGMCC under the number of CGMCC No.6993. A screening method of the Streptomycesbottropensis includes: 1, activating strains to be activated; 2, preparing a screening plate; 3, screening and decomposing lecithin strains by a plate method; 4, detecting transesterification activity. The Streptomycesbottropensis has the advantages that the operation process is simple, implementation conditions are mild, phosphatidylserine can be bio-converted efficiently and quickly, and basis of industrial production of phosphatidylserine in future is laid.