Method for extracting and purifying polysaccharide of winter cherry by ultrasonic waves

A technology of ultrasonic wave and physalis, applied in the field of preparation of extracting polysaccharides, can solve the problems of long time, low polysaccharide extraction rate and low efficiency, etc.

Inactive Publication Date: 2018-01-09
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
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Problems solved by technology

The extraction method is a traditional method for extracting polysaccharides from Physalis pulp, but it takes a long time, has low efficiency, and consumes a lot of energy. In addition, under the conditions of dilute acid and dilute

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  • Method for extracting and purifying polysaccharide of winter cherry by ultrasonic waves
  • Method for extracting and purifying polysaccharide of winter cherry by ultrasonic waves
  • Method for extracting and purifying polysaccharide of winter cherry by ultrasonic waves

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Embodiment Construction

[0027] The technical solution of the present invention will be further described below in conjunction with the accompanying drawings, but it is not limited thereto. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the present invention. within the scope of protection.

[0028] 1. Preparation of physalis powder

[0029] The wild Physalis is washed, beaten, dried, pulverized by a pulverizer and passed through a 60-mesh sieve to obtain Physalis powder, which is set aside.

[0030] 1. Single factor experimental design

[0031] The ratio of liquid to material (20:1, 25:1, 30:1, 35:1, 40:1), ultrasonic temperature (35, 40, 45, 50, 55°C), ultrasonic power (160, 200, 240 , 280, 320W) and ultrasonic time (10, 20, 30, 40, 50min) and other four factors on the extraction rate of Physalis polysaccharide.

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Abstract

The invention discloses a method for extracting and purifying physalis polysaccharides by using ultrasonic waves, and belongs to the field of preparation of extracted polysaccharides. The preparation method comprises the following steps: washing, beating, drying and pulverizing the physalis and passing through a 60-mesh sieve to obtain the physalis powder; after ultrasonic treatment under different conditions, centrifuging, and the supernatant is the polysaccharide extract. Through the single factor test, the optimum ratio of liquid to material, ultrasonic temperature, ultrasonic time, and ultrasonic power for extracting physalis polysaccharides were determined. Volume ratio of sample solution-sevage reagent, volume ratio of chloroform-n-butanol, shaking time, deproteinization times. Hydrogen peroxide decolorization, through a single factor test, finally determined the optimum decolorization temperature, solution pH value, decolorization time, hydrogen peroxide addition amount.

Description

technical field [0001] The invention relates to a method for extracting and purifying physalis polysaccharides by using ultrasonic waves, belonging to the field of preparation of polysaccharides extracted, and purifying the polysaccharides by using a sevage method for deproteinization and a hydrogen peroxide decolorization method. Background technique [0002] Physalis belongs to the Solanaceae family and is divided into two types: red Physalis and yellow Physalis. Red Physalis is also known as brocade lantern, hanging golden lanterns, the fruit is spherical, the berries are orange-red when ripe, and there are enlarged calyx outside the berries like a lantern. Traditional Chinese medicine believes that its nature and flavor are slightly bitter, and it belongs to the lung meridian. In our country, it is also popular among the people to use physalis to treat diabetes, which has a good effect of lowering blood sugar. At present, the research, development and application of Ph...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 于国萍孙琪刘鹏王世让刘艳秋董良伟
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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