Method for extracting lutein from Cordyceps militaris
A technology of Cordyceps militaris and lutein, applied in the direction of organic chemistry, can solve the problems of low utilization rate of artificial Cordyceps militaris and low extraction rate of lutein, and achieve suitable large-scale production, optimization of process parameters and extraction conditions, high efficiency The effect of extraction
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Embodiment 1
[0033] Embodiment 1: investigate the kind of ionic liquid
[0034] A. Prepare materials
[0035] Take 1 kg of artificial Cordyceps militaris, wash with water, dry naturally, then pulverize, grind, mix well, pass through a 20-mesh sieve, and collect artificial Cordyceps militaris powder.
[0036] B. Fermentation treatment
[0037] Weighing step A to obtain 100 g of artificial Cordyceps militaris powder and 0.5 g of Angel yeast powder, wherein Angel yeast powder is 0.5% of the weight of artificial Cordyceps militaris powder, dissolved in warm water at 35°C, and fermented for 20 hours at a constant temperature and isolated from the air. Obtain a fermented broth.
[0038] C. Ionic liquid extraction and purification
[0039] Filter the fermentation broth in step B using a filter with a mesh size of 150 mesh, add 800 g of ionic liquid to the filtrate, mix and mix, then add 40 g of extraction aid 18-crown-6, place in ultrasonic Extract in an extraction kettle at 300 W ultrasonic...
Embodiment 2
[0046] Embodiment 2: investigate the kind of extraction aid
[0047] A. Prepare materials
[0048] Take 1 kg of artificial Cordyceps militaris, wash with water, dry naturally, then pulverize, grind, mix well, pass through a 30-mesh sieve, and collect artificial Cordyceps militaris powder.
[0049] 、 Fermentation
[0050] Weighing step A to obtain 100 g of artificial Cordyceps militaris powder and 1 g of Angel yeast powder, wherein the Angel yeast powder is 1% of the weight of the artificial Cordyceps militaris powder, dissolved in warm water at 40°C, and fermented at a constant temperature and isolated from air for 18 hours. Obtain a fermented broth.
[0051] C. Ionic liquid extraction and purification
[0052] The fermented liquid of step B is filtered with a filter screen, the mesh of the filter screen used is 100 mesh, 1000 g of 1-butyl-3-methylimidazolium tetrafluoroborate is added to the filtrate, after mixing and mixing, add 40 g extraction aid, placed in an ultra...
Embodiment 3
[0059] Embodiment 3: investigate the consumption of ionic liquid
[0060] A. Prepare materials
[0061] Take 1 kg of artificial Cordyceps militaris, wash with water, dry naturally, then pulverize, grind, mix well, pass through a 40-mesh sieve, and collect artificial Cordyceps militaris powder.
[0062] B. Fermentation treatment
[0063] Weighing step A to obtain 100 g of artificial Cordyceps militaris powder and 1.5 g of Angel yeast powder, wherein Angel yeast powder is 1.5% of the weight of artificial Cordyceps militaris powder, dissolved in warm water at 40°C, and fermented for 22 hours at a constant temperature and isolated from the air. Obtain a fermented broth.
[0064] C. Ionic liquid extraction and purification
[0065] Filter the fermentation broth in step B using a filter with a mesh size of 150 mesh, add ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate to the filtrate, mix well, and then add extraction Auxiliary agent 18-crown-6, in which the amount o...
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