Curcumin extraction and purification method

Abstract

The invention discloses a curcumin extraction and purification method which comprises the following steps: weighing dry turmeric powder, adding an ethanol aqueous solution with the volume fraction ofgreater than or equal to 74%, and performing three times of extraction and three times of filtration, with a recycling extraction manner adopted; diluting the primary extraction filtrate until the ethanol content does not exceed 50%, and performing sample loading adsorption through a polyamide resin chromatographic column; washing the column with purified water of which the volume is three times that of the column to remove water-soluble impurities; washing the column with an ethanol aqueous solution with the volume fraction of 50%-70% to remove alcohol-soluble impurities; washing the column with an ethanol aqueous solution which is 5 times the volume of the column and which has a volume fraction of 60-75% to elute curcumin on the column, and collecting eluate; concentrating the collectedeluate to be dry, adding an ethanol aqueous solution to dissolve to obtain a saturated solution, crystallizing and separating out at 0 DEG C to room temperature, washing the crystals, and drying to obtain curcumin. The extraction and purification process is simple, the extraction and purification processes are easy to control, industrial application is easy, the turmeric extract with the total curcumin content larger than or equal to 95% can be prepared, and economic benefits are good.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of plant active ingredients, and in particular relates to an extraction and purification method of curcumin. Background technique [0002] Turmeric (Latin scientific name: Curcum a longa L.) is a commonly used traditional Chinese medicine. Curcumin (Curcumin) is an active ingredient extracted from turmeric. Wide range of pharmacological effects, and very low toxicity, LD in mice 50 >2g / kg. [0003] The existing extraction methods of curcumin include alkaline water extraction and enzyme extraction. However, the enzymatic extraction method has high requirements for extraction temperature and pH value, is difficult to control, and has a complicated process. At the same time, the damage to curcumin is greater under the alkaline adjustment of the alkaline water extraction method. In addition, in the existing separation and purification methods of curcumin, most of them need to ...

AI Technical Summary

Problems solved by technology

However, the enzymatic extraction method has high requirements for extraction temperature and pH value, is not easy to control, and the process is complicated

At the same time, under the alkaline adjustment of the alkaline water extraction method, the damage to curcumin is greater

In addition, in the existing separation and purification methods of curcumin, most of them need to use organic solvents with high toxic and side effects such as methanol, n-hexane, petroleum ether or ethyl acetate, resulting in high residual organic solvents in the separated products, which affect the production of curcumin. Applications in fields such as food and medicine

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