30results about How to "Simple extraction and purification process" patented technology

The invention relates to an extraction technology of natural pigment, in particular to a method for extracting purple sweet potato anthocyanin. The method comprises the following steps of: grinding purple sweet potatoes into powder and adding cellulase to carry out enzymolysis on the purple sweet potato powder; adding citric acid to substances subjected to the enzymolysis to carry out extraction;filtering the extract obtained in the extraction step through macroporous absorbing resin, eluting the macroporous absorbing resin which adsorbs purple sweet potato anthocyanin by using eluent and condensing the purple sweet potato anthocyanin subjected to the eluting; and carrying out spray drying on the condensed purple sweet potato anthocyanin pigment obtained in the purification step to obtain final products and detecting the quality of the final products. The purple sweet potato anthocyanin extracted by the method has the advantages of excellent quality, less impurity content, stable property and long shelf life, the number of recycling times of the macroporous absorbing resin is increased, and the production efficiency is improved.

Production of raphsulfane with brassin vegetables as raw materials is carried out by crushing for seeds, flowers, stems and leaves, hydrolyzing at specific pH value by myrosase, extracting by acetic ether, adsorbing by silica gel, removing impurities by acetic ether and eluting by mixed solution of isopropyl alcohol, alcohol and petroleum ether. It's cheap and simple and can be used for large-scale industrial production.

The invention provides a method for extracting epsilon-polylysine and salt thereof from fermentation liquid, which comprises the following steps: in an acidification tank, the fermentation liquid is acidized and then filtrated to obtain clear filtrate; and then the clean filtrate is alkalized, made into alkalified solution and then pressed into a resin adsorption column to be absorbed; firstly saturated resin is washed with purified water and then analyzed with hydrochloric acid to obtain analytic liquid which is decolorized and filtrated by activated carbon to obtain destaining solution; the destaining solution is pressed into a membrane filtration device to be circularly concentrated so as to obtain concentrated solution; the concentrated solution is sprayed or decompressed, frozen and dried to obtain the epsilon-polylysine pure product. The raw material and the instrument adopted in the method are easily obtained and have low cost; the extraction and purification technique is simple and practicable; and the final product has high yield and high purity. The prepared epsilon-polylysine can be used as preservative, biological high polymer material, micro-capsule technique material, food therapy agent, drug carriers, biological chips, high polymer nonabsorbent material and the like.

The invention relates to a preparation method and technology of alliin reference substance, which comprises the following steps of: first extracting alliin from fresh garlic; then preparing the alliin reference substance by using two methods: the first method is to prepare the alliin reference substance by ethanol recrystallization; and the second method is to prepare the alliin reference substance by acetone recrystallization; and finally determining the alliin physical constant, analyzing four spectrum, and determining the purity; if all the indicators thereof are eligible, the product complies with the 'alliin reference substance' required by the 'plant extract reference substance' regulated by the State Food and Drug Administration.

The invention belongs to the technical field of fermentation, and discloses a lysine production, extraction and purification process which includes the steps: 1) preparing seed solution; 2) preparinga fermentation medium; 3) producing lysine by fermentation; 4) performing extraction and purification. The process is low in cost and high in lysine yield and purity.

The invention relates to a method for preparing an antimalarial active compound artemisinin through direct column chromatography and belongs to the field of drug preparation techniques. The method comprises the steps of: weighing 1 part (by weight) of artemisia annua leaf powder which is sieved by a sieve with the particle size of 100 meshes, weighing 0.7-1.4 parts (by weight) of silica gel or alumina which is sieved by a sieve with the particle size of 80-100 meshes, mixing the artemisia annua leaf powder and the silica gel or alumina, and grinding uniformly; coating the substance, obtained in the last step, in a glass chromatography column containing 0.8-5.0 parts (by weight) of silica gel or alumina which is sieved by the sieve with the particle size of 80-100 meshes, eluting in a manner that petroleum ether-ethyl acetate, or petroleum ether-acetone, or chloroform-acetone, or chloroform-ethyl acetate or the like, which are in the volume ratio of 85: (10-20), serve as a moving phase, and collecting fractions; and carrying out depressurized concentration on a fraction containing artemisinin, and crystallizing by using petroleum ether, thereby obtaining white needle-shaped artemisinin crystals. Compared with the prior art, the method has the advantages that the extraction and purification process for artemisinin is optimized, the solvent extraction operating steps and the types of used solvents are reduced, and the consumption of the solvents is reduced; and the obtained product has the advantages of stable quality, high yield, low cost and short consumed time.

The invention provides malania oleifera lectin and a method for preparing the same. The method comprises the following steps: grinding malania oleifera, stirring and socking the ground malania oleifera into cold water, grading and precipitating by using ammonium sulfate, ultrafiltering to remove salt, concentrating, carrying out ion exchange chromatography, purifying, ultrafiltering to remove salt, concentrating, freezing and drying to obtain the refined malania oleifera lectin. The prepared malania oleifera lectin has the molecular weight of about 60kd, the electrophoresis purity of above 90% and the potency of 1:10<5> or more, namely the HIV infected cell (inhibition concentration 50) IC50 of 1:10<5>, can agglutinate the red blood cells of a rabbit, a pig, a chicken, a duck and a mouse and can be used for preventing HIV infection.

The invention relates to a method for extracting omega-6 polyunsaturated fatty acid from plant seeds, eliminating impurities from the plant seeds, properly preprocessing and then roughly crushing, making microwave vibration to break their walls and rapidly drying and finely crushing to make them into powder of a certain size, making supercritical CO2 fluid extraction on the made powder, selectively extracting, separating and purifying omega-6 polyunsaturated fatty acid in the raw material on proper conditions. The invention fully uses the raw material, and does not produce waste residues and waste steam and pollute the environment. The products have high physiological activity and excellent taste.

The invention relates to an extraction technique of a natural pigment, particularly an extraction process of a purple sweet potato anthocyanin pigment, which comprises the following steps: enzymolysis: pulverizing purple sweet potatoes into powder, and adding cellulase to perform enzymolysis on the purple sweet potato powder; leaching: adding citric acid into the enzymolysis product to perform leaching; purification: filtering the leaching solution obtained in the leaching step through a macroporous adsorbent resin so that the purple sweet potato anthocyanin pigment is adsorbed to the macroporous adsorbent resin, eluting the macroporous adsorbent resin with an eluting agent, and concentrating the purple sweet potato anthocyanin pigment obtained by elution; and drying and detection: carrying out spray drying on the concentrated purple sweet potato anthocyanin pigment in the purification step to obtain a finished product, and carrying out quality detection on the finished product. The purple sweet potato anthocyanin pigment extracted by the process has the advantages of good quality, greatly lower impurity content, higher property stability and long shelf life; and the process increases the number of times of reuse of the macroporous adsorbent resin and enhances the production efficiency.

The invention discloses a curcumin extraction and purification method which comprises the following steps: weighing dry turmeric powder, adding an ethanol aqueous solution with the volume fraction ofgreater than or equal to 74%, and performing three times of extraction and three times of filtration, with a recycling extraction manner adopted; diluting the primary extraction filtrate until the ethanol content does not exceed 50%, and performing sample loading adsorption through a polyamide resin chromatographic column; washing the column with purified water of which the volume is three times that of the column to remove water-soluble impurities; washing the column with an ethanol aqueous solution with the volume fraction of 50%-70% to remove alcohol-soluble impurities; washing the column with an ethanol aqueous solution which is 5 times the volume of the column and which has a volume fraction of 60-75% to elute curcumin on the column, and collecting eluate; concentrating the collectedeluate to be dry, adding an ethanol aqueous solution to dissolve to obtain a saturated solution, crystallizing and separating out at 0 DEG C to room temperature, washing the crystals, and drying to obtain curcumin. The extraction and purification process is simple, the extraction and purification processes are easy to control, industrial application is easy, the turmeric extract with the total curcumin content larger than or equal to 95% can be prepared, and economic benefits are good.

The invention relates to a method for preparing gamma-aminobutyric acid by using continuous circulation of 732 resin to assist the immobilized cells, and belongs to the technical field of green bio-manufacturing. The method is characterized in that 732 cation exchange resin columns I and II arranged in parallel are respectively and alternatively used as auxiliary reaction columns by valve control to be coupled with immobilized enterococcus faecium cell columns, and are connected with other auxiliary facilities or equipment to form a circular reaction system; high-efficiency catalytic reaction is carried out by circulation of primers between the 732 resin auxiliary reaction columns and the immobilized enterococcus faecium cell columns, so that the yield of gamma-aminobutyric acid is increased; and while in conversion reaction assisted by one 732 resin column, the other 732 resin column is diluted, regenerated and balanced, so that continuous production of the immobilized enterococcus faecium cell columns under assistance of the 732 resin columns is realized. Compared with a single-column reaction method only adopting the immobilized cells, the method provided by the invention has the advantage that under the conditions of same reaction time and equivalent immobilized enterococcus faecium, the GABA yield in single-batch circulation is increased by 178.83%.

The invention relates to a traditional Chinese medicine for treating metrorrhagia and metrostaxis, gastrointestinal hemorrhage and traumatic hemorrhage and a preparation method thereof. The traditional Chinese medicine comprises the following raw material components in part by weight: 24 to 48 parts of the extract of rhubarb and India madder root in the weight ratio of 1:1, 10 to 20 parts of notoginseng root extract, 4 to 12 parts of hide glue, 4 to 12 parts of myrrh volatile oil inclusion compound, 10 to 20 parts of common bletilla pseudobulb extract and 2 to 5 parts of dragon's blood. The preparation method comprises the following steps of: extracting all the extracts; mixing the extracts; adding lactose and aerosol; and mixing and filling the mixture to obtain capsules, or adding talcpowder, magnesium stearate, carboxymethyl sodium starch and the aerosol, uniformly mixing and directly tabletting. The traditional Chinese medicine has the advantages of simple and controllable process, short production cycle, low cost, exact curative effect of products, safety, no toxic or side effect, stable quality, convenient storage, contribution to clinical popularization and application of medical products, and obvious clinical therapeutic economic significance.

The invention belongs to the technical field of sugar separation and purification, and discloses a method for separating and purifying lactose from whey. The method comprises the steps that the lactose is placed into a vessel, heating is carried out to 70-75 DEG C, heat preservation is conducted, then centrifugation is carried out, a supernatant liquid is collected, then adsorbent is added into the supernatant liquid, stirring is conducted, then standing and filtering are conducted, and a filter liquid is collected; the filter liquid is filtered through nanofiltration membrane, and a filteredliquid is collected; the filtered liquid is concentrated, crystallized and dried to obtain the lactose. The method for separating and purifying the lactose from the whey has the advantages that the extraction and purification method is simple, the purity degree of the lactose is high, and the operability is high.

The invention relates to a trimethylol ethane three-effect evaporation concentration crystallization system and application thereof, and belongs to the technical field of concentration crystallization, wherein a one-effect evaporator forms a circulation path with a one-effect crystallizer and a one-effect circulation pump through pipelines; a two-effect evaporator forms a circulation path with a two-effect crystallizer and a two-effect circulation pump through pipelines; a three-effect evaporator forms a circulation path with a three-effect crystallizer and a three-effect circulation pump through pipelines; a heat exchanger feeding opening is communicated with a feeding pipeline; a discharging opening is communicated with a feeding opening of the three-effect evaporator through a pipeline.The design and the invention are performed by aiming at the production process and features of trimethylol ethane; a set of trimethylol ethane three-effect evaporation concentration crystallization system and a method for performing trimethylol ethane three-effect evaporation concentration crystallization by using the system are developed. An evaporation concentration technology, a multi-effect evaporation technology and an evaporation crystallization technology are effectively combined; the purification process route of the trimethylol ethane is greatly shortened.

The invention discloses a method for obtaining piperine via direct column chromatography, which belongs to the technical field of medicine preparation. The method comprises the following steps of: taking 1 part by weight of pepper powder passing through a 100-mesh sieve, and 0.8-1.2 parts by weight of silica gel or aluminium oxide with a particle size of 80-100 meshes, uniformly mixing the two raw materials and grinding; spreading the obtained product in a glass chromatography column filled with 0.8-5.0 parts by weight of silica gel or aluminium oxide with a particle size of 80-100 meshes, eluting by taking petroleum ether-acetone or petroleum ether-ethyl acetate or chloroform-acetone or chloroform-ethyl acetate in a volume ratio of 5: (0.5 to 2.0) as a mobile phase, and collecting fractions; and performing reduced pressure concentration on the fractions containing piperine, and then crystallizing by methanol-water in a volume ratio of (7.8 to 8.2): 2 to obtain faint yellow piperine massive crystals. Compared with the prior art, the method disclosed by the invention has the advantages that extraction and purification processes of piperine are optimized, the operation steps of solvent extraction and the types of the used solvents are reduced, and the consumption of the solvents is decreased; and moreover, the obtained product is stable in quality, high in productivity, low in cost, and low in time consumption.

The invention relates to a method for extracting omega-6 polyunsaturated fatty acid from plant seeds, eliminating impurities from the plant seeds, properly preprocessing and then roughly crushing, making microwave vibration to break their walls and rapidly drying and finely crushing to make them into powder of a certain size, making supercritical CO2 fluid extraction on the made powder, selectively extracting, separating and purifying omega-6 polyunsaturated fatty acid in the raw material on proper conditions. The invention fully uses the raw material, and does not produce waste residues and waste steam and pollute the environment. The products have high physiological activity and excellent taste.

The invention belongs to the technical field of sugar separation and purification, and discloses a technology for preparing isomerizated lactose fluid with whey. The technology comprises the followingsteps of (1) preparing an adsorbing agent; (2) performing heating treatment, centrifuging treatment and adsorption treatment; (3) performing nanofiltration; and (4) performing concentration, crystallization and isomerization. The technology is simple to operate, low in cost and high in purity of prepared products.

The invention discloses a method for preparing L-2,4-diaminobutyric acid. L-2,4-diaminobutyric acid is prepared by hydrolyzing, separating and drying γ-polydiaminobutyric acid; wherein, The above-mentioned hydrolysis method is pressure hydrolysis, or organic acid hydrolysis, or acidic resin combined with sodium nitrite hydrolysis, or protease hydrolysis. The method of the present invention has simple process, convenient operation, high efficiency and no need for decolorization. It is the first domestic and foreign summary of the production process of L-2,4-diaminobutyric acid, a product with huge application prospects.

The invention belongs to the technical field of plant extraction and discloses a method for extracting anthocyanin by utilizing a deep eutectic solvent (DES). The method comprises the following steps: uniformly mixing raw material powder containing anthocyanin with the DES, stirring the materials at 30-60 DEG C for 20-60 minutes to enable the materials to react fully, then taking supernatant through centrifugation, and carrying out elution and drying after the supernatant is adsorbed by a macroporous absorption resin column, thus obtaining anthocyanin powder, wherein the DES is a eutectic mixture of quaternary ammonium salts and hydrogen bond donors in a mole ratio of 1 to (10-19). The method has the beneficial effects that the DES can dissolve anthocyanin more effectively as the solubility parameters of the hydrogen bond donors in the DES are very close to the solubility parameters of the basic structure of anthocyanin, while the phase transfer catalysis effects of the hydrogen bond donors (quaternary ammonium salts) have better promoting effects on dissolution of anthocyanin; and therefore, high-purity anthocyanin can be efficiently purified by utilizing the DES.

The invention provides a method for extracting ε-polylysine and its salt from fermentation broth. In the acidification tank, the fermentation broth is acidified and then filtered to obtain a clear filtrate, and then the clear filtrate is alkalized to make an alkalized solution Then press it into the resin adsorption column for adsorption, first wash the saturated resin with purified water, and then analyze it with hydrochloric acid to obtain the analysis solution, decolorize the analysis solution with activated carbon, filter to obtain the decolorization solution, and put the decolorization fluid into the membrane filter device for circulation and concentration. The concentrated solution is obtained, and the concentrated solution is sprayed or freeze-dried under reduced pressure to obtain the pure product of ε-polylysine. The raw materials and instruments used in the invention are easy to obtain and low in cost; the extraction and purification process is simple and feasible; and the final product has high yield and high purity. The prepared ε-polylysine can be used as preservative, biopolymer material, microcapsule technology material, drug carrier, food therapy agent, biochip and polymer water-absorbing material, etc.

The present invention relates to the active polysaccharide extracted and purified from Agaricus bisporus canned wastewater and its extraction and purification process and application. The active polysaccharide Abap1001 extracted and purified from Agaricus bisporus processed wastewater is composed of arabinose and xylose as monosaccharides , mannose and glucose, the active polysaccharide Abap1002 extracted and purified from the canned processing wastewater of Agaricus bisporus, its monosaccharide composition is arabinose, xylose, mannose and glucose, an extraction and purification process of active polysaccharide, which includes the following Process steps: material pretreatment, ion exchange column chromatography, Sephadex G-200 gel column chromatography, the active polysaccharide Abap1001 and the active polysaccharide Abap1002 of the present invention have good biological activity, and the purification process of the present invention is simple and easy.

The invention discloses linseed oil capable of improving hyperlipidemia, hypertension and hyperglycemia and a preparation method of the linseed oil. The preparation method comprises the following steps: (1) degumming of linseeds, (2) preparation of linseed pulp, (3) preparation of linseed oil coarse purification substances by enzymolysis, (4) acid removal, and (5) sterilizing. The linseed oil capable of improving hyperlipidemia, hypertension and hyperglycemia has good storage stability and taste; a processing technology is simple and is safe to operate; the yield of the linseed oil is high; and the raw materials are saved effectively. By operation on the technology, the content of VE with effect of improving hyperlipidemia, hypertension and hyperglycemia in the linseed oil is increased, and the health-care effect of the linseed oil is enhanced.

The invention provides malania oleifera lectin and a method for preparing the same. The method comprises the following steps: grinding malania oleifera, stirring and socking the ground malania oleifera into cold water, grading and precipitating by using ammonium sulfate, ultrafiltering to remove salt, concentrating, carrying out ion exchange chromatography, purifying, ultrafiltering to remove salt, concentrating, freezing and drying to obtain the refined malania oleifera lectin. The prepared malania oleifera lectin has the molecular weight of about 60kd, the electrophoresis purity of above 90% and the potency of 1:10<5> or more, namely the HIV infected cell (inhibition concentration 50) IC50 of 1:10<5>, can agglutinate the red blood cells of a rabbit, a pig, a chicken, a duck and a mouse and can be used for preventing HIV infection.

The invention discloses a method for extracting gamma-polydiaminobutyric acid and polylysine from fermentation liquor, which comprises the following steps of: pretreating the fermentation liquor, adsorbing and desorbing by using resin, decolorizing, concentrating, and drying; and performing drying treatment respectively to obtain the finished products of the polylysine and the polydiaminobutyric acid respectively. Raw materials and instruments are readily available and low in cost; an extraction and purification process is simple and feasible; the finished products have high yield and purity;and the method is environment-friendly, and suitable for industrial mass production.

The invention relates to a guanine peptide nucleic acid monomer. A structural formula of the guanine peptide nucleic acid monomer is as shown in the specification. A preparation method of the guanine peptide nucleic acid monomer comprises the following steps: (1), under a condition that potassium carbonate is taken as alkaline, reacting a compound G0 with benzyl bromoacetate to produce a compound Ga; 2, reacting the compound Ga with triphosgene and reacting the obtained product with diphenyl carbinol to produce Gb; and (3), reacting Gb with cyanosodium ethoxide to produce Gc; and (4), reacting Gc with Fmoc-aminoethyl glycine to produce a target product. According to the synthetic method, the used raw materials are very low in price, and very low in cost, the steps are few, the operation is very simple, the extracting and purifying process is relatively convenient, and the yield and the purity, which respectively reach 98% and over 50%, of the obtained guanine peptide nucleic acid monomer are relatively high. And moreover, the defects in the existing synthetic process are overcome.

Production of raphsulfane with brassin vegetables as raw materials is carried out by crushing for seeds, flowers, stems and leaves, hydrolyzing at specific pH value by myrosase, extracting by acetic ether, adsorbing by silica gel, removing impurities by acetic ether and eluting by mixed solution of isopropyl alcohol, alcohol and petroleum ether. It's cheap and simple and can be used for large-scale industrial production.