Preparation method for L-2,4-diaminobutyric acid

A technology of diaminobutyric acid and polydiaminobutyric acid, which is applied in the field of bioengineering, can solve problems such as the production of γ-polydiaminobutyric acid, and achieve the effects of low cost, easy equipment acquisition, and simple extraction and purification process

Inactive Publication Date: 2013-11-20
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report on the production of L-2,4-diaminobutyric acid directly from γ-polydiaminobutyric acid at home and abroad

Method used

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  • Preparation method for L-2,4-diaminobutyric acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Free fermentation of S.albulus PD-1 in a 5L tank to produce polylysine and polydiaminobutyric acid

[0045] Incline medium: glucose 10g / L, beef extract 5g / L, K 2 HPO 4 1g / L, MgSO 4 ·7H 2 O 0.5g / L, agar 20g / L, pH 7.0.

[0046] Seed medium: glucose 10g / L, beef extract 5g / L, yeast extract 5g / L, K 2 HPO 4 1g / L, MgSO 4 ·7H 2 O0.5g / L, utilize 6mol / LNaOH solution to adjust pH 7.0

[0047] Fermentation medium: glucose 50g / L, yeast extract 5g / L, (NH 4 ) 2 SO 4 5g / L, Na 2 HPO 4 12H 2 O 1.58g / L, K 2 HPO 4 ·7H 2 O 0.82g / L, MgSO 4 ·7H 2 O 0.1g / L, use 6mol / L NaOH solution to adjust the pH to 7.0.

[0048] Cultivate Streptomyces albus S. albulus PD-1 (CCTCC NO: M2011043) in the seed medium, 28°C, 200r / min shaker conditions for 24h, and 300mL of seed liquid was mixed with 10% (v / v) seed The amount of inoculation was carried out in a fermenter pre-installed with 2.7L sterilized fermentation medium for free culture. The culture conditions were: 28°C, 400...

Embodiment 2

[0049] Embodiment 2: Separation and extraction of polydiaminobutyric acid.

[0050] In the acidification tank, use 3mol / L hydrochloric acid to adjust the pH value of the fermented liquid prepared in Example 1 to 2.0, then heat it to 60°C, keep it warm for 20 minutes, cool the acidified liquid to room temperature, filter it through a plate and frame, and re-filter it to obtain Clear the filtrate. Use 6mol / L sodium hydroxide solution to adjust the pH value of the filtrate to 9.0 to obtain a pretreatment solution.

[0051] Pump the pretreatment liquid into the adsorption column of weakly acidic cation exchange resin (110) with an aspect ratio of 20:1 for adsorption. During the adsorption process, the flow rate is controlled at 4mL / min (about 2BV / h) until the resin adsorption reaches saturation. Wash the saturated resin with deionized water, control the flow rate to 6mL / min (about 3BV / h), and wash the volume to 3 column volumes. Analyze with pre-prepared 3mol / L hydrochloric acid...

Embodiment 3

[0055] Put γ-polydiaminobutyric acid into a 200g / L aqueous solution and put it into the reactor, seal the equipment, heat and pressurize for 6 hours under the conditions of temperature 180°C, pressure 0.8Mpa, stirring speed 120 rpm , reduce the pressure to normal pressure. After the hydrolyzate evaporates, it is spray-dried under the conditions of an inlet air temperature of 160° C. and an outlet air temperature of 70° C. to obtain a solid powder of L-2,4-diaminobutyric acid. The purity and yield of the obtained L-2,4-diaminobutyric acid were 96% and 98%, respectively.

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Abstract

The invention discloses a preparation method for L-2,4-diaminobutyric acid. The method is performed through hydrolyzing gamma-poly(diaminobutyric acid), separating and drying, wherein the hydrolyzing process comprises pressure hydrolysis, or organic acid hydrolysis, or acidic resin combined sodium nitrite hydrolysis, or protease hydrolysis. The preparation method provided by the invention has the advantages of simple process, high efficiency and no need for decoloring treatment, and is convenient to operate, is the first summary of the production process for L-2,4-diaminobutyric acid with excellent application prospect at home and abroad.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for preparing L-2,4-diaminobutyric acid. Background technique [0002] L-2,4-diaminobutyric acid (2,4-diaminobutyric acid, English abbreviation DABA), also known as 2,4-butyric acid, is a secondary non-protein amino acid, which belongs to the four-carbon compound, and its structural formula (NH 2 )CH 2 CH 2 CH(NH 2 )COOH is a basic amino acid. It widely exists in animals, plants and microorganisms, and has unique physiological functions. L-2,4-diaminobutyric acid has good application prospects in the pharmaceutical industry and agriculture. In terms of medicine, an appropriate amount of L-2,4-diaminobutyric acid is a kind of drug. Studies have found that L-2,4-diaminobutyric acid can cause hyperosmotic pressure and is toxic to mouse fibroma cells. Human malignant glioma cells have a lytic effect, therefore, L-2,4-diaminobutyric acid can be used ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C229/26C07C227/20C12P13/04
Inventor 徐虹冯小海张扬夏军孙芸李莎欧阳平凯
Owner NANJING TECH UNIV
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