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Method for extracting epsilon-polylysine

A polylysine and concentration technology, applied in animal feed, animal feed, animal husbandry, etc., can solve the problems of low yield, cumbersome extraction process, and low content, and achieve low cost, easy equipment and simple process Effect

Inactive Publication Date: 2013-06-19
SHANDONG SINDER TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for extracting ε-polylysine, so as to solve the problems of cumbersome extraction process, low yield, low content and high cost.

Method used

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  • Method for extracting epsilon-polylysine
  • Method for extracting epsilon-polylysine

Examples

Experimental program
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Effect test

Embodiment 1

[0032] The fermented liquid just out of the tank has a pH of 3.0-5.0. First heat the fermented liquid to 80°C, keep it warm for 15 minutes, then cool it to 30°C, add diatomaceous earth for adsorption, first use a decanter centrifuge to centrifuge for the first time, and then use The tube centrifuge is used for second centrifugation, and after the bacterial cells are removed by centrifugation, a clear filtrate is obtained. Add a sodium hydroxide solution with a concentration of 5 mol / L to adjust the pH of the filtrate to 8.5 to obtain an alkalized solution. After cooling, put the alkalized hydraulic pressure into the weakly acidic cation exchange resin adsorption column for adsorption. In this embodiment, 152 resin is preferred. The height to diameter ratio of the adsorption column is 5:1. Sodium, to ensure that the pH value of the adsorption process remains at 8.5 until the resin adsorption reaches saturation. Wash with purified water and 0.2mol / L acetic acid solution twice r...

Embodiment 2

[0035] The fermented liquid just out of the tank has a pH of 3.0-5.0. First heat the fermented liquid to 60°C, keep it warm for 30 minutes, cool to 10°C, add diatomaceous earth for adsorption, first use a decanter centrifuge to centrifuge for the first time, and then use a tube Type centrifuge carries out the second centrifugation, and after centrifugation removes bacterium, obtains clear filtrate. Add a sodium hydroxide solution with a concentration of 5 mol / L to adjust the pH of the filtrate to 10.0 to obtain an alkalized solution. After cooling, put the alkalized hydraulic pressure into the weakly acidic cation exchange resin adsorption column for adsorption. In this embodiment, HD-2 resin is preferred. The height to diameter ratio of the adsorption column is 10:1. The adsorption process controls the flow rate to 4BV / h while continuously adding hydrogen Sodium oxide, to ensure that the pH value of the adsorption process remains at 10.0 until the resin adsorption reaches sat...

Embodiment 3

[0038] The fermented liquid just out of the tank has a pH of 3.0-5.0. First heat the fermented liquid to 100°C, keep it warm for 5 minutes, cool to 60°C, add diatomaceous earth for adsorption, first use a decanter centrifuge to centrifuge for the first time, and then use a tube Type centrifuge carries out the second centrifugation, and after centrifugation removes bacterium, obtains clear filtrate. Add a sodium hydroxide solution with a concentration of 5 mol / L to adjust the pH of the filtrate to 6.0 to obtain an alkalized solution. After cooling, put the alkalized hydraulic pressure into the weakly acidic cation exchange resin adsorption column for adsorption. In this embodiment, HD-2 resin is preferred. The height and diameter ratio of the adsorption column is 2:1. Sodium hydroxide is used to ensure that the pH value of the adsorption process remains at 6.0 until the resin adsorption reaches saturation. Wash with purified water and 0.5mol / L acetic acid solution twice respec...

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Abstract

The invention relates to a method for extracting epsilon-polylysine. The method comprises the following steps of: adsorbing a fermentation broth with diatomite, and centrifuging to obtain a clear filtrate; alkalizing the clear filtrate; adding the alkalized clear filtrate into a resin adsorption column for adsorbing; washing a saturated resin, and resolving to obtain a solution of resolving; decoloring the solution of resolving with activated charcoal, filtering, concentrating, and drying by spraying or drying by crystallizing to obtain the pure epsilon-polylysine. The raw materials and the instruments adopted by the method are easily available, the process is simple, the cost is low, and the obtained product has high yield and high purity.

Description

technical field [0001] The invention relates to the extraction technology of preservatives, in particular to a method for extracting ε-polylysine (ε-poly-L-lysine, ε-PL) from fermentation broth. Background technique [0002] Corruption and deterioration of feed will cause huge economic losses, how to prevent corruption is a problem that scientists are most concerned about. At present, the commonly used antiseptic measure is to add feed preservatives. Feed preservatives are divided into chemical preservatives, natural preservatives and compound preservatives, among which chemical preservatives are the most widely used. With the gradual improvement of people's awareness and requirements for food safety, chemical preservatives are faced with severe challenges, and the naturalization of feed anti-mold preservatives has become a development trend in the future. [0003] ε-polylysine is a new type of natural anti-mildew preservative, which is not only harmless to human health, bu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G69/08A23K1/16
Inventor 李朝阳李常青乔彦良
Owner SHANDONG SINDER TECH
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