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Method for extracting gamma-polydiaminobutyric acid and polylysine from fermentation liquor

A technology of polydiaminobutyric acid and polylysine, which is applied in the field of separation engineering, can solve the problems that plague the industrial production of ε-PL, low product concentration, and low production efficiency, and achieve low cost, high purity, and product yield high effect

Inactive Publication Date: 2012-09-26
NANJING TECH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the production process of ε-PL, problems such as low production efficiency, low product concentration, and difficult product separation have plagued the industrial production of ε-PL.

Method used

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  • Method for extracting gamma-polydiaminobutyric acid and polylysine from fermentation liquor
  • Method for extracting gamma-polydiaminobutyric acid and polylysine from fermentation liquor
  • Method for extracting gamma-polydiaminobutyric acid and polylysine from fermentation liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Free fermentation of S.albulus PD-1 in a 5L tank to produce polylysine and polydiaminobutyric acid

[0042] Incline medium: glucose 10g / L, beef extract 5g / L, K 2 HPO 4 1g / L, MgSO 4 ·7H 2 O 0.5g / L, agar 20g / L, pH 7.0.

[0043] Seed medium: glucose 10g / L, beef extract 5g / L, yeast extract 5g / L, K 2 HPO 4 1g / L, MgSO 4 ·7H 2 O0.5g / L, utilize 6mol / LNaOH solution to adjust pH 7.0

[0044] Fermentation medium: glucose 50g / L, yeast extract 5g / L, (NH 4 ) 2 SO 4 5g / L, Na 2 HPO 4 12H 2 O 1.58g / L, K 2 HPO 4 ·7H 2 O 0.82g / L, MgSO 4 ·7H 2 O 0.1g / L, use 6mol / L NaOH solution to adjust the pH to 7.0.

[0045] Cultivate Streptomyces albus S. albulus PD-1 (CCTCC NO: M2011043) in the seed medium, 28°C, 200r / min shaker conditions for 24h, and 300mL of seed liquid was mixed with 10% (v / v) seed The amount of inoculation was carried out in a fermenter pre-installed with 2.7L sterilized fermentation medium for free culture. The culture conditions were: 28°C, 400...

Embodiment 2

[0048] In the acidification tank, use 3mol / L hydrochloric acid to adjust the pH value of the fermentation broth to 2.0, then heat it to 60°C, keep it warm for 20 minutes, cool the acidification solution to room temperature, filter it through a plate and frame, and obtain a clear filtrate after repeated filtration. Use 6mol / L sodium hydroxide solution to adjust the pH value of the filtrate to 9.0 to obtain a pretreatment solution.

[0049] Pump the pretreatment liquid into the adsorption column of weakly acidic cation exchange resin (110) with an aspect ratio of 20:1 for adsorption. During the adsorption process, the flow rate is controlled at 4mL / min (about 2BV / h) until the resin adsorption reaches saturation. Wash the saturated resin with deionized water, control the flow rate to 6mL / min (about 3BV / h), and wash the volume to 3 column volumes. Analyze with pre-prepared 3mol / L hydrochloric acid, control the flow rate at 1mL / min (about 0.5BV / h), and collect polydiaminobutyric ac...

Embodiment 3

[0053] In the acidification tank, use 2mol / L hydrochloric acid to adjust the pH value of the fermentation broth to 5.0, then heat the acidification solution to 100°C, keep it warm for 10 minutes, cool the acidification solution to room temperature, filter it through a plate frame, and obtain a clear filtrate after repeated filtration. Add 6mol / L sodium hydroxide to the clear filtrate, adjust the pH of the clear filtrate to 7.2 to obtain a pretreatment solution.

[0054] Pump the pretreatment solution into the macroporous strong acid ion exchange resin (D001) adsorption column (height-to-diameter ratio: 10:1) for adsorption. During the adsorption process, the flow rate should be controlled at 4mL / min (about 4BV / h) until the resin adsorbs reach saturation. Wash the saturated resin with 5 times the column volume of deionized water at a flow rate of 5 mL / min (about 5 BV / h). Use pre-prepared 1mol / L acetic acid aqueous solution for analysis, control the analysis rate of 1mL / min (ab...

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Abstract

The invention discloses a method for extracting gamma-polydiaminobutyric acid and polylysine from fermentation liquor, which comprises the following steps of: pretreating the fermentation liquor, adsorbing and desorbing by using resin, decolorizing, concentrating, and drying; and performing drying treatment respectively to obtain the finished products of the polylysine and the polydiaminobutyric acid respectively. Raw materials and instruments are readily available and low in cost; an extraction and purification process is simple and feasible; the finished products have high yield and purity;and the method is environment-friendly, and suitable for industrial mass production.

Description

technical field [0001] The invention belongs to the technical field of separation engineering, and in particular relates to a method for extracting gamma-polydiaminobutyric acid and polylysine from fermentation broth. Background technique [0002] γ-polydiaminobutyric acid is a homopolymer formed by polymerizing the γ-amino and α-carboxyl groups of L-2,4-diaminobutyric acid. The current research results show that γ-polydiaminobutyric acid has similar properties to polylysine in terms of thermal stability and water solubility. It is worth noting that γ-polydiaminobutyric acid has a strong yeast inhibitory ability , and has some inhibitory G + Bacteria, G - Bacteria and mold activity, is a potential excellent biological preservative. Its monomer diaminobutyric acid (2,4-diaminobutyric acid, English abbreviation DABA), also known as 2,4-butyric acid, is a secondary non-protein amino acid, which belongs to the four-carbon compound, and its structural formula is (NH 2 )CH 2 ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G69/10
Inventor 徐虹张扬冯小海夏军倪芳李莎欧阳平凯
Owner NANJING TECH UNIV
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