Process method for preparing gardenia iridoid glycosides from leaves of gardenia plants

Abstract

The invention discloses a process for preparing gardenia iridoid glycosides from the leaves of gardenia plants. The specific process steps are as follows: (1) add beating agent to fresh gardenia leaves, or add extractant to dried gardenia leaf powder to make gardenia leaf slurry; (2) wash gardenia leaf slurry Stir and wash with the agent for more than one time, then filter or centrifuge, combine and retain the supernatant or filtrate to obtain the active ingredient extract of gardenia iridoid glycosides; (3) Concentrate the extract and elute it on a chromatographic column to obtain Gardenia iridoid glycoside compound, off-white or white powder, of which geniposide is 20-99.9%. The method of the invention can prepare a large amount of gardenia iridoid glycoside compounds mainly composed of geniposide at low cost. The present invention utilizes gardenia leaves to produce gardenia iridoid glycoside active ingredients, which can greatly reduce the production cost compared with the traditional production method using gardenia fruit as raw material, and the raw material cost is only 1 / 50 of the traditional cost.

Description

technical field [0001] The invention belongs to the technical field of extraction and preparation of biological products, and relates to a preparation process for active ingredients of gardenia iridoid glycosides. Background technique [0002] Gardenia iridoid glycosides are the main active ingredients of the internationally recognized traditional Chinese medicine Gardenia. Such ingredients have always been extracted from the dried and ripe fruits of Rubiaceae gardenia. Gardenia fruit is a traditional Chinese medicine, and it is also a Chinese characteristic product that China has vigorously developed in recent years and intends to enter the international market. In addition to iridoid glycosides such as geniposide or geniposide, gardenia fruit also contains a large amount of gardenia pigments. Among them, gardenia iridoid glycosides have multiple uses: they can be made into natural food coloring gardenia blue, and they are also raw materials for treating diseases such as ...

AI Technical Summary

Problems solved by technology

[0003] Gardenia iridoid glycosides have broad application fields, but the traditional preparation method is to extract from Gardenia fruit, and Gardenia fruit contains a large amount of interfering substances, resulting in complex extraction process, excessive material consumption, and high yield. The yield is low, the cost of raw materials is also high, and the product is extremely expensive, which limits the large-scale application of this type of composition and the development of related industries in my country in recent years