Process method for preparing gardenia iridoid glycosides from leaves of gardenia plants

A technology of iridoid glycosides and process methods, which is applied in the field of extraction and preparation of biological products, can solve the problems of restricting a large number of applications, complex extraction processes, and high raw material costs, and achieve improved chromatographic separation efficiency, reduced process costs, and simplified extraction and purification processes Effect

Active Publication Date: 2017-08-25
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Gardenia iridoid glycosides have broad application fields, but the traditional preparation method is to extract from Gardenia fruit, and Gardenia fruit contains a large amount of interfering substances, resulting in complex extraction process, excessive material consumption, and high yield. The yield is low, the cost of raw materials is also high, and the product is extremely expensive, which limits the large-scale application of this type of composition and the development of related industries in my country in recent years

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The specific operation steps of preparing gardenia iridoid glycosides from gardenia leaves are as follows:

[0032] 1. Preparation of Gardenia Leaf Pulp

[0033] 100 kg fresh gardenia ( Gardenia jasminoides Ellis) leaves and 20 kg of water (solid-to-liquid ratio 1:0.2 (w / w)) were mixed and beaten at a temperature of 10°C for 600 minutes to obtain 120 kg of gardenia leaf pulp;

[0034] (2) Preparation of Gardenia iridoid glycosides extract

[0035] Centrifuge 120 kg of gardenia leaf pulp at a speed of 3000 rpm for 30 minutes to obtain 60 kg of centrifugal sediment, add 12 kg of water (solid-liquid ratio 1:0.2 (w / w)) to the 60 kg of centrifugal sediment, stir and wash for 1 time, then centrifuged again under the condition of rotating speed 3000 revs / min for 5 minutes, and a total of 80 kilograms of centrifuged supernatants were combined twice to be the extract of gardenia iridoid glycosides;

[0036] (3) Preparation of iridoid glycosides

[0037] Concentrate the extr...

Embodiment 2

[0039] The specific operation steps of preparing gardenia iridoid glycosides from gardenia leaves are as follows:

[0040] 1. Preparation of Gardenia Leaf Pulp

[0041] 100 kg of fresh water gardenia ( Gardenia jasminoides var. radicans Makino ) leaves and 300 kg of 5% ethanol (solid-to-liquid ratio 1:3) were mixed and beaten at a temperature of 98°C for 10 minutes to obtain 400 kg of gardenia leaf pulp;

[0042] (2) Preparation of the extract of gardenia iridoid glycosides

[0043] Centrifuge 400 kg of gardenia leaf pulp at 10,000 rpm for 5 minutes to obtain 60 kg of centrifugal sediment, add 120 kg of 5% ethanol (solid-liquid ratio 1:2) to the 60 kg of centrifugal sediment, stir and wash twice , and were centrifuged at 10,000 rpm for 30 minutes respectively, and a total of 460 kilograms of centrifuged supernatants combined three times was the extract of gardenia iridoid glycosides;

[0044] (3) Preparation of iridoid glycosides

[0045] Concentrate the extract of 460 ki...

Embodiment 3

[0047] The specific operation steps of preparing gardenia iridoid glycosides from gardenia leaves are as follows:

[0048] 1 Preparation of gardenia leaf slurry

[0049] 100 kg of dried gardenia ( Gardenia jasminoides Ellis) leaves were beaten into leaf powder, mixed with 100 kg of 95% methanol (solid-to-liquid ratio 1:1), and extracted at a temperature of 10°C for 600 minutes to obtain 200 kg of gardenia leaf pulp;

[0050] (2) Preparation of the extract of gardenia iridoid glycosides

[0051] Centrifuge 200 kg of gardenia leaf pulp at a speed of 5000 rpm for 15 minutes to obtain 150 kg of centrifugal sediment, add 30 kg of 95% methanol (solid-liquid ratio 1:0.2) to the 150 kg of centrifugal sediment, stir and wash, pass through 20 mesh Sieve, the centrifugation supernatant that merges secondary is the extract of Gardenia iridoid glycosides compound, totally 80 kilograms;

[0052] (3) Preparation of iridoid glycosides

[0053] Concentrate the extract of 80 kilograms of gar...

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Abstract

The invention discloses a process for preparing gardenia iridoid glycosides from the leaves of gardenia plants. The specific process steps are as follows: (1) add beating agent to fresh gardenia leaves, or add extractant to dried gardenia leaf powder to make gardenia leaf slurry; (2) wash gardenia leaf slurry Stir and wash with the agent for more than one time, then filter or centrifuge, combine and retain the supernatant or filtrate to obtain the active ingredient extract of gardenia iridoid glycosides; (3) Concentrate the extract and elute it on a chromatographic column to obtain Gardenia iridoid glycoside compound, off-white or white powder, of which geniposide is 20-99.9%. The method of the invention can prepare a large amount of gardenia iridoid glycoside compounds mainly composed of geniposide at low cost. The present invention utilizes gardenia leaves to produce gardenia iridoid glycoside active ingredients, which can greatly reduce the production cost compared with the traditional production method using gardenia fruit as raw material, and the raw material cost is only 1 / 50 of the traditional cost.

Description

technical field [0001] The invention belongs to the technical field of extraction and preparation of biological products, and relates to a preparation process for active ingredients of gardenia iridoid glycosides. Background technique [0002] Gardenia iridoid glycosides are the main active ingredients of the internationally recognized traditional Chinese medicine Gardenia. Such ingredients have always been extracted from the dried and ripe fruits of Rubiaceae gardenia. Gardenia fruit is a traditional Chinese medicine, and it is also a Chinese characteristic product that China has vigorously developed in recent years and intends to enter the international market. In addition to iridoid glycosides such as geniposide or geniposide, gardenia fruit also contains a large amount of gardenia pigments. Among them, gardenia iridoid glycosides have multiple uses: they can be made into natural food coloring gardenia blue, and they are also raw materials for treating diseases such as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/04C07H1/08A61K36/744A61K127/00
Inventor 胡丰林
Owner ANHUI AGRICULTURAL UNIVERSITY
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