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Extraction process of purple sweet potato anthocyanin pigment

A purple sweet potato anthocyanin and pigment technology, which is applied in azo dyes, organic dyes, chemical instruments and methods, etc., can solve the problem of low extraction rate of purple sweet potato anthocyanin pigment, low quality of purple sweet potato anthocyanin pigment, and poor quality of purple sweet potato anthocyanin pigment. It is easy to cause problems such as pollution of purification equipment, so as to achieve the effect of optimizing the extraction and purification process, high quality and reduced impurity content.

Inactive Publication Date: 2015-03-25
QINGDAO ZHONGREN ZHIYE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the direct extraction of fresh potatoes, this method has relatively many impurities, which is easy to cause pollution and damage to the purification equipment, which will have a negative impact on subsequent purification, and the extraction rate of purple sweet potato anthocyanin pigments in the prior art is relatively low. , resulting in greater waste; prior art
[0005] The quality of the purple sweet potato anthocyanin pigment extracted in the operation is not high, so the purple sweet potato anthocyanin pigment market has been in a state of high price and low quality

Method used

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  • Extraction process of purple sweet potato anthocyanin pigment
  • Extraction process of purple sweet potato anthocyanin pigment
  • Extraction process of purple sweet potato anthocyanin pigment

Examples

Experimental program
Comparison scheme
Effect test

experiment Embodiment 1

[0036] Experimental example 1: the pH value of anthocyanin pigment solution is set to 4.0, 4.5, 5.0, 5.5, 6.0 respectively, other conditions are temperature 25 ℃, enzyme amount 1.5%, substrate concentration 1: 5, enzymolysis time 30min, detect the absorbance of anthocyanin pigment solution after enzymatic hydrolysis, and calculate the color value of anthocyanin pigment solution, draw figure 2 The curve shown. Depend on figure 2 It can be seen that acidic conditions are conducive to the enzymatic hydrolysis of purple sweet potato cell membrane by cellulase, thereby releasing more anthocyanin pigments.

experiment Embodiment 2

[0037] Experimental Example 2: Set the enzymolysis extraction reaction time to 15min, 30min, 45min, 60min, 75min respectively, other conditions are temperature 25°C, enzyme addition 1.5%, substrate concentration 1:5, pH value 4.0, enzymolysis Detect the absorbance of anthocyanin pigment solution after finishing, and calculate the color value of anthocyanin pigment solution, draw image 3 The curve shown.

[0038] Depend on image 3 It can be seen that the color value decreases after 30 minutes of reaction. This is because the cellulase acts for a long time and destroys the anthocyanin pigment, so the most suitable reaction time for enzymatic hydrolysis is determined to be 30 minutes.

experiment Embodiment 3

[0039] Experimental example 3: the enzymolysis extraction reaction temperature is respectively set to 25 ℃, 30 ℃, 35 ℃, 40 ℃, 45 ℃, other conditions are reaction time 30min, enzyme amount 1.5%, substrate concentration 1: 5, pH Value 4.0, detect the absorbance of anthocyanin pigment solution after enzymolysis, and calculate the color value of anthocyanin pigment solution, draw Figure 4 The curve shown. Depend on Figure 4 It can be seen that the color value is the lowest when the temperature is 30°C, indicating that the cellulose destroys anthocyanin pigments at this temperature. After that, the extraction rate increases with the increase of temperature, but the increase is slow after 40°C. In order to ensure a high extraction rate and make the present invention more energy-saving, 40° C. is the optimum temperature.

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Abstract

The invention relates to an extraction technique of a natural pigment, particularly an extraction process of a purple sweet potato anthocyanin pigment, which comprises the following steps: enzymolysis: pulverizing purple sweet potatoes into powder, and adding cellulase to perform enzymolysis on the purple sweet potato powder; leaching: adding citric acid into the enzymolysis product to perform leaching; purification: filtering the leaching solution obtained in the leaching step through a macroporous adsorbent resin so that the purple sweet potato anthocyanin pigment is adsorbed to the macroporous adsorbent resin, eluting the macroporous adsorbent resin with an eluting agent, and concentrating the purple sweet potato anthocyanin pigment obtained by elution; and drying and detection: carrying out spray drying on the concentrated purple sweet potato anthocyanin pigment in the purification step to obtain a finished product, and carrying out quality detection on the finished product. The purple sweet potato anthocyanin pigment extracted by the process has the advantages of good quality, greatly lower impurity content, higher property stability and long shelf life; and the process increases the number of times of reuse of the macroporous adsorbent resin and enhances the production efficiency.

Description

technical field [0001] The invention relates to a technology for extracting natural pigments, in particular to a method for extracting purple sweet potato anthocyanin pigments. Background technique [0002] As people pay more attention to food safety, the state has stepped up efforts to crack down on the illegal use of synthetic pigments in food and cosmetics, which has given people a new understanding of the irreplaceable superiority of natural pigments. Purple sweet potato anthocyanin is an important natural pigment discovered at the end of the last century and widely used in food, cosmetics and health products in developed countries. Purple sweet potato anthocyanin pigment is a mixed pigment of various anthocyanins, which has better [0003] In addition to photothermal stability, it also has strong anti-liposome peroxidation ability and the ability to scavenge hydroxyl free radicals in the human body. Natural purple sweet potato anthocyanin has many advantages: non-toxi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00
CPCC09B61/00
Inventor 谭超智
Owner QINGDAO ZHONGREN ZHIYE BIOTECH
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