Method for obtaining piperine via direct column chromatography

Abstract

The invention discloses a method for obtaining piperine via direct column chromatography, which belongs to the technical field of medicine preparation. The method comprises the following steps of: taking 1 part by weight of pepper powder passing through a 100-mesh sieve, and 0.8-1.2 parts by weight of silica gel or aluminium oxide with a particle size of 80-100 meshes, uniformly mixing the two raw materials and grinding; spreading the obtained product in a glass chromatography column filled with 0.8-5.0 parts by weight of silica gel or aluminium oxide with a particle size of 80-100 meshes, eluting by taking petroleum ether-acetone or petroleum ether-ethyl acetate or chloroform-acetone or chloroform-ethyl acetate in a volume ratio of 5: (0.5 to 2.0) as a mobile phase, and collecting fractions; and performing reduced pressure concentration on the fractions containing piperine, and then crystallizing by methanol-water in a volume ratio of (7.8 to 8.2): 2 to obtain faint yellow piperine massive crystals. Compared with the prior art, the method disclosed by the invention has the advantages that extraction and purification processes of piperine are optimized, the operation steps of solvent extraction and the types of the used solvents are reduced, and the consumption of the solvents is decreased; and moreover, the obtained product is stable in quality, high in productivity, low in cost, and low in time consumption.

Description

technical field

[0001] The invention belongs to the technical field of medicine preparation, and relates to a method for extracting biologically active compounds from plants, in particular to extracting piperine (piperine) from Piper nigrum L., a plant of the family Piperaceae. Background technique:

[0002] Pepper (Piper nigrum L.) is a plant belonging to the family Piperaceae. It is mainly produced in tropical regions such as Hainan, Yunnan, and Vietnam in my country. It is the most widely used seasoning in the food industry. Piperine is the main active substance in pepper, which belongs to amide alkaloids, and has a wide range of pharmacological effects such as sedation, liver protection, anti-tumor, immune regulation, anti-oxidation, anti-ulcer, anti-depression, anti-convulsion, and improving biological drug utilization rate, stimulating melanocyte proliferation, etc. (Liu Ping, Suo Jingxia, Yu Tengfei, Chinese Drug Application and Monitoring, 2007, (3): 7-9).

[0003] ...

Examples

Embodiment

[0040] Embodiment: active compound adopts following method and operating condition to prepare. Specifically: dried pepper powder, passed through a 100-mesh sieve. Take 1 g of pepper powder (100 mesh), mix it with 1 g of silica gel (80-100 mesh), grind it evenly, and cover it in a glass chromatography column (30×1 cm) filled with 3.0 g of silica gel (80-100 mesh) , eluting with petroleum ether: acetone (5: 1) as the mobile phase, and collecting fractions (4 ml as a fraction). Fractions 6-11 were concentrated under reduced pressure and crystallized with methanol-water (8:2) to obtain 8 mg of piperine in pale yellow blocky crystals. The yield was 0.8 percent.

technical field

[0001] The invention belongs to the technical field of medicine preparation, and relates to a method for extracting biologically active compounds from plants, in particular to extracting piperine (piperine) from Piper nigrum L., a plant of the family Piperaceae. Background technique:

[0002] Pepper (Piper nigrum L.) is a plant belonging to the family Piperaceae. It is mainly produced in tropical regions such as Hainan, Yunnan, and Vietnam in my country. It is the most widely used seasoning in the food industry. Piperine is the main active substance in pepper, which belongs to amide alkaloids, and has a wide range of pharmacological effects such as sedation, liver protection, anti-tumor, immune regulation, anti-oxidation, anti-ulcer, anti-depression, anti-convulsion, and improving biological drug utilization rate, stimulating melanocyte proliferation, etc. (Liu Ping, Suo Jingxia, Yu Tengfei, Chinese Drug Application and Monitoring, 2007, (3): 7-9).

[0003] ...

Examples

Embodiment

[0040] Embodiment: active compound adopts following method and operating condition to prepare. Specifically: dried pepper powder, passed through a 100-mesh sieve. Take 1 g of pepper powder (100 mesh), mix it with 1 g of silica gel (80-100 mesh), grind it evenly, and cover it in a glass chromatography column (30×1 cm) filled with 3.0 g of silica gel (80-100 mesh) , eluting with petroleum ether: acetone (5: 1) as the mobile phase, and collecting fractions (4 ml as a fraction). Fractions 6-11 were concentrated under reduced pressure and crystallized with methanol-water (8:2) to obtain 8 mg of piperine in pale yellow blocky crystals. The yield was 0.8 percent.

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