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Lysine production, extraction and purification process

A lysine and process technology, applied in the field of amino acid fermentation, can solve the problems of long process route, large consumption of sulfuric acid, large consumption of raw and auxiliary materials, etc., and achieves the effects of simple purification process, improved fermentation efficiency and low waste water production.

Pending Publication Date: 2018-11-13
齐齐哈尔龙江阜丰生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the existing process for extracting and purifying lysine, a large amount of sulfuric acid needs to be consumed when adjusting the pH of the lysine clear liquid, a large amount of liquid ammonia will be consumed when eluting with ammonia water, and a large amount of liquid ammonia will be consumed when the resin is washed with water. A large amount of waste water will be generated, which will increase the burden on environmental protection and cause a waste of resources. In addition, the resin will be broken and lost during the separation process, which requires high performance of the separation equipment.
This process is long, the consumption of raw and auxiliary materials is large, and the cost is high, which restricts the development of the lysine industry

Method used

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  • Lysine production, extraction and purification process

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Experimental program
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Effect test

Embodiment 1

[0035] The production, extraction and purification process of lysine, it comprises the following steps:

[0036] Corynebacterium glutamicum ATCC14997 was selected as the fermentation strain; Corynebacterium glutamicum was inserted into the seed medium and cultivated to a density of 3×10 8 The seed liquid of cfu / mL; The component of described seed culture medium is: glucose 80g / L, yeast extract 30g / L, corn steep liquor 10g / L, potassium dihydrogen phosphate 2g / L, dipotassium hydrogen phosphate 2g / L, Ferrous sulfate heptahydrate 0.2g / L, magnesium sulfate heptahydrate 0.2g / L, biotin 10mg / L, vitamin B1 2mg / L;

[0037] Inoculate the seed liquid into the fermenter containing the fermentation medium according to the inoculum amount of 10%, and carry out aerobic fermentation. The fermentation temperature is 32°C, the tank pressure is 0.05MPa, and the fermentation liquid is obtained by fermentation for 48 hours. During the fermentation process, chlorine is continuously added Ammonium ...

Embodiment 2

[0046] The production, extraction and purification process of lysine, it comprises the following steps:

[0047] Corynebacterium glutamicum ATCC14997 was selected as the fermentation strain; Corynebacterium glutamicum was inserted into the seed medium and cultivated to a density of 5×10 8 The seed liquid of cfu / mL; The component of described seed culture medium is: glucose 80g / L, yeast extract 30g / L, corn steep liquor 10g / L, potassium dihydrogen phosphate 2g / L, dipotassium hydrogen phosphate 2g / L, Ferrous sulfate heptahydrate 0.2g / L, magnesium sulfate heptahydrate 0.2g / L, biotin 10mg / L, vitamin B 1 2mg / L;

[0048] Inoculate the seed liquid into the fermenter containing the fermentation medium according to the inoculation amount of 10%, and carry out aerobic fermentation. The fermentation temperature is 35°C, the tank pressure is 0.05MPa, and the fermentation liquid is obtained by fermentation for 60 hours. During the fermentation process, chlorine is continuously added Ammo...

Embodiment 3

[0057] Contents of each main component in the fermentation medium of the present invention:

[0058] A control group was set up to test the influence of strains on the content of the main components of the culture medium. Control group 1: only Trichoderma viride was used; Control group 2: only Bacillus licheniformis was used. The specific results are shown in Table 1:

[0059] Table 1

[0060] Composition index

[0061] As shown in Table 1, compared with the control group 1-2 using a single strain, the medium components obtained by the combined treatment of Trichoderma viride and Bacillus licheniformis are richer and more comprehensive, which is in line with the use of Corynebacterium glutamicum for acid production. standard.

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Abstract

The invention belongs to the technical field of fermentation, and discloses a lysine production, extraction and purification process which includes the steps: 1) preparing seed solution; 2) preparinga fermentation medium; 3) producing lysine by fermentation; 4) performing extraction and purification. The process is low in cost and high in lysine yield and purity.

Description

technical field [0001] The invention belongs to the technical field of amino acid fermentation, and in particular relates to the production, extraction and purification process of lysine. Background technique [0002] Lysine is an amino acid needed by the human body, an indispensable nutrient. Protein is the main component of human cells. After entering the human body, protein in food is first decomposed into amino acids after digestion, and then the human body uses these amino acids to synthesize new human proteins, such as immune antibodies, digestive enzymes, plasma proteins, growth hormones, etc. It is a synthetic human protein. Among the various amino acids that synthesize proteins, L-lysine is the most important one. Without lysine, other amino acids are limited or cannot be used. Scientists call it the first essential amino acid for the human body. Scientists have also discovered that L-lysine is the most important and essential component of somatostatin, an impor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P13/08C12N1/14C12N1/20C12R1/885C12R1/10
CPCC12N1/14C12N1/20C12P13/08C12P39/00
Inventor 王峰唐永强汲广习聂庆胜董力青邱庆志李航王铮张丽微姚志强
Owner 齐齐哈尔龙江阜丰生物科技有限公司
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