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A method for extracting anthocyanins using a deep eutectic solvent

A deep eutectic solvent, anthocyanin technology, applied in chemical instruments and methods, organic chemistry, organic dyes, etc., to achieve high-efficiency extraction, reduce pollution, and reduce volatilization.

Active Publication Date: 2020-11-13
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research on the use of deep eutectic solvents to extract anthocyanins has not yet appeared at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Put the alder leaf Tangdi rich in anthocyanins into the container, then add DES (butyltrimethylammonium chloride: lactic acid=1:15, mol / mol) and mix well, the raw material rich in anthocyanins and DES The mass-to-liquid ratio is 1:20g / mL, stirred at 45°C and 120r / min for 20min to make the reaction fully, then centrifuged at 12000r / min for 5min to take the supernatant, adsorbed by AB-8 macroporous adsorption resin column, and then washed with water Add 5 times 0.1% HCl acidified 80% ethanol aqueous solution to the resin column to elute the active ingredients, collect the eluate, concentrate and freeze-dry to obtain total anthocyanins with a content of 72.2%.

Embodiment 2

[0024] Put the alder leaf Tangdi rich in anthocyanins into the container, then add DES (tetrabutylammonium chloride: lactic acid = 1:15, mol / mol) and mix evenly, the raw material rich in anthocyanins and the mass liquid of DES The ratio is 1:20g / mL, stir at 45°C and 120r / min for 20min to make the reaction fully, then centrifuge at 12000r / min for 5min to take the supernatant, absorb it through the AB-8 macroporous adsorption resin column, and then wash the resin column with water , and then add 5 times of 0.1% HCl acidified 80% ethanol aqueous solution to elute the active ingredients, collect the eluate, concentrate and freeze-dry to obtain the total anthocyanins with a content of 78.9%.

Embodiment 3

[0026] The alder leaf Tangdi that is rich in anthocyanins is put into container, then adds DES (choline chloride: lactic acid=1:10, mol / mol) and mixes evenly, the mass-to-liquid ratio of the raw material that is rich in anthocyanins and DES is 1:15g / mL, stir at 45°C and 120r / min for 30min to make the reaction fully, then centrifuge at 12000r / min for 5min to take the supernatant, absorb it through the AB-8 macroporous adsorption resin column, then wash the resin column with water, and then Add 3 times of 0.1% HCl acidified 80% ethanol aqueous solution to elute the active ingredients, collect the eluate, concentrate and freeze-dry to obtain total anthocyanins with a content of 80.7%.

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Abstract

The invention belongs to the technical field of plant extraction and discloses a method for extracting anthocyanin by utilizing a deep eutectic solvent (DES). The method comprises the following steps: uniformly mixing raw material powder containing anthocyanin with the DES, stirring the materials at 30-60 DEG C for 20-60 minutes to enable the materials to react fully, then taking supernatant through centrifugation, and carrying out elution and drying after the supernatant is adsorbed by a macroporous absorption resin column, thus obtaining anthocyanin powder, wherein the DES is a eutectic mixture of quaternary ammonium salts and hydrogen bond donors in a mole ratio of 1 to (10-19). The method has the beneficial effects that the DES can dissolve anthocyanin more effectively as the solubility parameters of the hydrogen bond donors in the DES are very close to the solubility parameters of the basic structure of anthocyanin, while the phase transfer catalysis effects of the hydrogen bond donors (quaternary ammonium salts) have better promoting effects on dissolution of anthocyanin; and therefore, high-purity anthocyanin can be efficiently purified by utilizing the DES.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a method for extracting anthocyanins by using a deep eutectic solvent. Background technique [0002] With the development of science and technology and the improvement of living standards, the demand for high-quality natural products has been increasing day by day in recent years. Anthocyanin is a water-soluble pigment that can make flowers and fruits appear in various colors. Anthocyanin is a powerful antioxidant, which can protect the human body from the damage of a harmful substance called free radicals. Modern medicine has proved that anthocyanin has stronger scavenging freedom than vitamins and catechins. It has attracted much attention because of its ability to enhance vision, eliminate eye fatigue, delay brain aging, and enhance cardiopulmonary function (J. Agric. Food Chem. 2007, 87, 2665). Therefore, the commercial value of fruits rich in anthocyan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62C09B61/00
CPCC07D311/62C09B61/00
Inventor 娄文勇杨馥溪宗敏华华洋林梁静
Owner SOUTH CHINA UNIV OF TECH
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