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Extraction method of polygonatum polysaccharide

A Polygonatum polysaccharide and extraction method technology, applied in the field of extraction, can solve the problems of cumbersome operation, heavy pollution, high energy consumption, etc., and achieve the effects of high extraction rate, high extraction purity, and optimized extraction conditions

Inactive Publication Date: 2020-11-24
上海铮信生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are currently many methods for polysaccharide extraction, but compared with the traditional "water extraction and alcohol precipitation" method of polysaccharides, there are a series of problems such as high energy consumption, large pollution, cumbersome operations, and expensive instruments.

Method used

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  • Extraction method of polygonatum polysaccharide
  • Extraction method of polygonatum polysaccharide
  • Extraction method of polygonatum polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for extracting Polygonatum Polysaccharides, comprising the following steps:

[0031] 1. Weigh 2 kg of dried sealwort decoction pieces, soak for 24 hours, add distilled water according to the ratio of material to liquid 1:10, select the extraction temperature as 70°C, heat and reflux for 1 hour, under the condition of extraction once, concentrate the filtrate and use 95% ethanol Precipitate, freeze-dry after standing overnight, and calculate the extraction rate of crude polysaccharide to be 18.5%.

[0032] 2. Protein removal

[0033] Take Polygonatum polysaccharide 1g, dissolve it to 20mg / mL, mix it with chloroform-n-butanol (5 / 1) solution at a ratio of 5:1, shake vigorously for 30min, the protein denatures to form a gel, and centrifuge to remove the denatured protein in the middle layer .

[0034] 3. Decolorization

[0035] Add 1% activated carbon to Polygonatum polysaccharide solution, decolorize it in a water bath at 100°C for 80 minutes, stir once every 1...

Embodiment 2

[0037] A method for extracting Polygonatum Polysaccharides, comprising the following steps:

[0038] 1. Weigh 2 kg of dried sealwort decoction pieces, soak for 24 hours, add distilled water according to the ratio of material to liquid 1:10, select the extraction temperature to be 80°C, heat and reflux for 2 hours, under the condition of extraction twice, concentrate the filtrate and use 95% ethanol Precipitate, freeze-dry after standing overnight, and calculate the extraction rate of crude polysaccharide to be 19.6%.

[0039] 2. Protein removal

[0040] Take Polygonatum polysaccharide 1g, dissolve it to 20mg / mL, mix it with chloroform-n-butanol (5 / 1) solution at a ratio of 5:1, shake vigorously for 30min, the protein denatures to form a gel, and centrifuge to remove the denatured protein in the middle layer .

[0041] 3. Decolorization

[0042] Add 2% activated carbon to Polygonatum polysaccharide solution, decolorize it in a water bath at 100°C for 80 minutes, stir once ev...

Embodiment 3

[0044] A method for extracting Polygonatum Polysaccharides, comprising the following steps:

[0045] 1. Weigh 2 kg of dried sealwort decoction pieces, soak for 24 hours, add distilled water according to the ratio of material to liquid 1:20, select the extraction temperature to be 80°C, heat and reflux for 3 hours, under the condition of extracting once, concentrate the filtrate and use 95% ethanol Precipitate, freeze-dry after standing overnight, and calculate the extraction rate of crude polysaccharide to be 27.8%.

[0046] 2. Protein removal

[0047] Take Polygonatum polysaccharide 1g, dissolve it to 20mg / mL, mix it with chloroform-n-butanol (5 / 1) solution at a ratio of 5:1, shake vigorously for 30min, the protein denatures to form a gel, and centrifuge to remove the denatured protein in the middle layer .

[0048] 3. Decolorization

[0049] Add 2% activated carbon to Polygonatum polysaccharide solution, decolorize it in a water bath at 100°C for 70 minutes, stir once eve...

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Abstract

The invention discloses an extraction method of polygonatum polysaccharide. The extraction method includes the steps that S1, dried polygonatum polysaccharide decoction pieces are weighed and crushed,and distilled water is added for soaking for 24 hours; S2, distilled water is added according to a certain material-to-liquid ratio, and centrifuging and concentrating are carried out after refluxingextraction; S3, 95% ethanol is added into a concentrate, putting overnight is carried out, and centrifugation and freeze-drying are carried out to obtain crude polysaccharide; S4, the polygonatum crude polysaccharide is taken, dissolved, mixed with chloroform-n-butanol, shaking vigorously is carried out for 30 minutes, protein is denatured to form a gel, and centrifuging is carried out to removethe denatured protein in a middle layer; and S5, activated carbon is added to a polygonatum polysaccharide solution, decolorizing is carried out, stirring is carried out once every 10 minutes, add absolute ethanol is added into an extract solution to enable the ethanol concentration in the mixed solution reach 80%, standing overnight is carried out, and centrifuging and freeze-dry are carried outto obtain the polygonatum polysaccharide. The extraction method is simple to operate, economical and feasible; and through a plurality of extractions, the extraction method has the advantages of higher extraction rate and less impurities.

Description

technical field [0001] The invention relates to an extraction method, in particular to an extraction method of polygonatum polysaccharide. Background technique [0002] With the continuous progress of society and the continuous improvement of living standards, people's pursuit of beauty is becoming more and more stringent. At present, natural, green and additive-free cosmetics are becoming more and more popular. It is a general trend to apply the active ingredients of plants in nature to cosmetics. With the development of the times, traditional Chinese medicine has received people's attention again with its long history. If the active ingredients in traditional Chinese medicine can be combined with modern cosmetics, it will not only greatly meet people's needs for natural and green cosmetics, but also give Chinese medicine new connotations. [0003] Polygonatum Polygonatum, also known as Chicken Head Polygonatum, Yellow Chicken Vegetable, Pen Tube Vegetable, Claw Ginseng,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 熊中立梁锋
Owner 上海铮信生物科技股份有限公司
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